Novel Optical Biosensor Based on a Nano-Gold Coated by Schiff Base Doped in Sol/Gel Matrix for Sensitive Screening of Oncomarker CA-125.

The urge for sensitive, facile, minimally invasive, and fast detection method of CA-125, a significant and crucial biomarker in ovarian malignancy, is currently substantial. This paper describes the detailed construction and characterization of a newly designed optical nano-biosensor to detect CA-125 accurately and sensitively. The fabricated sensor consists of a nano-gold thin film doped into a matrix of sol-gel, exhibiting a centered fluorescence band at 423 nm when excited at 340 nm. The quantification of CA-125 relies on its quenching ability of this fluorescence signal. The sensor was challenged to evaluate its sensitivity and specificity in detecting CA-125 present in samples collected from ovarian cancer diagnosed patients and compared to samples from healthy women as a control. Our findings revealed that the developed biosensor had a sensitivity of 97.35% and a specificity of 94.29%. Additionally, a wide linearity range over 2.0-127.0 U mL-1 for CA-125 was achieved with a detection limit of 1.45 U mL-1. Furthermore, the sensor could successfully discriminate samples between healthy and diseased people, which demonstrates its suitability in CA-125 assessment.

Using a single cone-beam computed tomography scan to obtain full occlusal details, with the mandible in centric relation and maximum intercuspation.

INTRODUCTION: One obstacle with the implementation of digital technology in orthodontics is the acquisition of occlusal details while maintaining the mandible in the full interdigitation position. With 1 cone-beam computed tomography (CBCT) scan of the patient and 1 digital scan of the patient's dental models in occlusion, it might be possible to obtain full occlusal details and generate 2 volumes, with the mandible in centric relation and maximum intercuspation positions. The purpose of this article was to describe the technique. METHODS: Orthodontic models of enrolled subjects were digitized in maximum intercuspation. They wore splints that were initially 2 mm thick before CBCT imaging. On the CBCT volume, the mandible is separated from the rest of the skull, and the digitized dental model in maximum intercuspation is registered on the skull volume. The separated mandibular volume is then registered on the mandibular dentition using the tooth surfaces as a guide. RESULTS: Two CBCT volumes are rendered: original scan with teeth in minimal disclusion and the constructed one with teeth in maximum interdigitation. CONCLUSIONS: Mobilizing digital technology in orthodontics allows the acquisition of crucial occlusal details while sparing patients an extra radiation dose from the CBCT.