A2/B1 Promotes NRF2 mRNA Stability and Inhibits Ferroptosis and Cell Proliferation in Breast Cancer Cells.

Breast cancer is a highly harmful malignant tumor, which poses a great threat to women's body and mind, and the mortality rate ranks second among all women's diseases. The incidence rate accounts for 7-10% of various malignant tumors in the whole body, second only to uterine cancer in women, and has become the main cause of threatening women's health. Advanced breast cancer is often considered an incurable disease. The family of heterogeneous nuclear ribonucleoprotein complexes is composed of about 20 hnRNP proteins with molecular weights ranging from 32 to 120 kDa, and they are named according to their molecular weights. Among them, hnRNPA2 and hnRNPB1 are the two most important members of the hnRNP family, both derived from the same gene on chromosome 7p15. Therefore, research to understand the molecular mechanism and process of breast cancer progression has an important role in promoting the current medical research on breast cancer treatment methods. Therefore, studying the mechanism of tumorigenesis is the key to tumor prevention and treatment. Therefore, this paper proposes that A2/B1 promotes the stability of NRF2 mRNA and inhibits ferroptosis and cell proliferation in breast cancer cells. The article mainly introduces the disease diagnosis method based on artificial neural network and its neural network algorithm. In the experimental part, the activity of hnRNP A2/B1 on cancer cells is deeply studied. The results show that the absorbance of the MTT method increases continuously with the extension of the culture time, and the maximum reaches 1.2. This fully shows that its absorption capacity is very strong, especially after 24 hours, the absorption rate rises from 0.6 to 0.9, which shows that 24 hours is the best absorption time. And it can also be found that hnRNPA2/B1 has a significant inhibitory effect on breast cancer cells; it can reduce the effect on breast cancer cell cycle and apoptosis.

A randomised controlled trial: effect of the meticulous nursing model on the treatment compliance and quality of life of patients with upper gastrointestinal bleeding.

BACKGROUND: The purpose of this study was to analyze the effect of the meticulous nursing model on the treatment compliance and quality of life of patients with upper gastrointestinal bleeding (UGIB). METHODS: A total of 108 UGIB patients treated in Linyi Central Hospital from October 2018 to October 2019 were selected as the study subjects, and were randomly divided into a research group and reference group, with 54 cases in each group. The reference group received conventional nursing while the research group received meticulous nursing on this basis to compare the clinical intervention effect and the impact on quality of life in the 2 groups of patients. RESULTS: The Generic Quality of Life Inventory-74 (GQOLI-74) scores in the 2 groups of patients after intervention were significantly higher than those before intervention (P<0.001), and the score of the research group after intervention was significantly higher than that of the reference group (P<0.001). The Stanford Acute Stress Reaction Questionnaire (SASRQ) scores of the patients presented a trend opposite to GQOLI-74 (P<0.001). The number of fully satisfied cases in the research group was significantly higher than that in the reference group (P<0.05), while the number of dissatisfied cases was significantly lower than that in the reference group (P<0.05). The self-rating anxiety scale (SAS) scores in the 2 groups of patients after intervention were significantly lower than those before intervention (P<0.001), and the score of the research group after intervention was significantly lower than that of the reference group (P<0.001). The total clinical effective rate and treatment compliance of the research group were significantly higher than those of the reference group (P<0.05). CONCLUSIONS: The meticulous nursing model can effectively improve the quality of life of UGIB patients, reduce the psychological stress response, and improve clinical treatment compliance and nursing satisfaction with a definite effect, making it worthy of promotion and application. TRIAL REGISTRATION: Chinese Clinical Trial Registry ChiCTR2100048735.

Effect and Nursing Satisfaction of Bedside Nursing Combined with Detail Nursing in Clinical Nursing of Gastroenterology Department.

OBJECTIVE: The purpose of the study was to investigate the therapeutic effect and nursing satisfaction of bedside nursing combined with detail nursing in the gastroenterology department. METHODS: 112 patients with gastrointestinal diseases admitted to our hospital from November 2018 to November 2019 were selected as the study subjects and randomly divided into a research group (n = 56) and reference group (n = 56). The reference group received routine clinical nursing, while on this basis, the research group received bedside nursing combined with detail nursing. After that, the clinical nursing effects of the two groups were compared. RESULTS: There were no significant differences in sex ratio, age, BMI, smoking history, drinking history, marital status, and disease types between the two groups (P > 0.05). The VAS scores in the two groups after intervention were significantly lower than those before intervention (P < 0.01), and the VAS scores in the research group after intervention were significantly lower than those in the reference group (P < 0.01). The nursing ability, nursing skills, and nursing responsibility in the research group were significantly higher than those in the reference group (P < 0.01). There were no significant differences between the two groups in the number of patients who were satisfied and needed improvement (P > 0.05). Besides, the number of very satisfied cases in the research group was significantly higher than that in the reference group (P < 0.05), and the number of unsatisfied cases was significantly lower than that in the reference group (P < 0.05). The total incidence of clinical adverse events in the research group was significantly lower than that in the reference group (P < 0.01). The gastrointestinal diseases related knowledge scores after intervention were significantly higher than those before intervention (P < 0.01), and the gastrointestinal diseases related knowledge scores after intervention in the research group were significantly higher than those in the reference group (P < 0.01). The GQOLI-74 scores after intervention in the two groups were significantly higher than those before intervention (P < 0.01), and the GQOLI-74 scores after intervention in the research group were significantly higher than those in the reference group (P < 0.01). CONCLUSION: The application of bedside nursing mode combined with detail nursing in gastrointestinal diseases can effectively reduce patients' pains, as well as the incidence of clinical adverse events, and improve patients' life quality, with definite curative effect, which is worthy of promotion and application.

Comparison of Immediate and Sequential Withdrawal of a Systemic Glucocorticoid in the Treatment of Acute Exacerbations of Chronic Obstructive Pulmonary Disease: A Multicenter, Randomized, Double-Blind, Parallel-Controlled, Open-Label Study.

Patients with acute exacerbations of chronic obstructive pulmonary disease (AECOPD) were treated with immediate or sequential withdrawal after 5 days of systemic glucocorticoids. The effects of the two withdrawal methods on the prognosis of patients were compared at 30, 90, 180, and 360 days after discharge. A multicenter, randomized, double-blind, parallel-controlled, open-label study was conducted in the respiratory department of tertiary hospitals in Central China. Patients met inclusion criteria for AECOPD and needed to use systemic glucocorticoids. They were randomly assigned to immediate and sequential withdrawal groups at a 1:1 ratio. The study was completed in August 2020 and is registered at the China Clinical Trials Registry (Chictr.org) (ChiCTR1800018894). According to general data and clinical characteristics, there were no statistically significant differences between the 329 patients in the immediate withdrawal group and the 310 patients in the sequential withdrawal group (P > 0.05). At the 30, 90, 180, and 360-days follow-up, the acute exacerbation frequency, rehospitalization rate, mortality, and intensive care unit (ICU) treatment rate were not significantly different between the immediate withdrawal group and sequential withdrawal group (P > 0.05). The modified Medical Research Council (mMRC) and COPD assessment test (CAT) scores were also not significantly different between the two groups. At the 180- and 360-day follow-up, forced expiratory volume in 1 s (FEV1%) and peak expiratory flow (PEF) were not significantly different between the two groups (P > 0.05). The time from discharge to first acute exacerbation was significantly lower in the immediate withdrawal group (46.12 days) than in sequential withdrawal group (49.02 days) (P < 0.05). The time of stay in the hospital for the first time after discharge was not significantly different between the two groups (P > 0.05). Adverse events were not significantly different between the immediate withdrawal group and sequential withdrawal group (P < 0.05). Subgroup analysis was performed according to age, degree of disease, and relevant indicators. At the 30-day follow-up, the acute exacerbation frequency of patients with advanced age, high global strategy for chronic obstructive lung disease (GOLD), and high fractional exhaled nitric oxide was significantly higher in the immediate withdrawal group than in the sequential withdrawal group (P < 0.05). In addition, according to receiver operating characteristic (ROC) curve analysis, the frequency of acute exacerbations at the 30-day follow-up was significantly higher in patients with age > 63.5 years or GOLD > 3 in the immediate withdrawal group than in the sequential withdrawal group, suggesting that the short-term efficacy was poor.

microRNAs-107 inhibited autophagy, proliferation, and migration of breast cancer cells by targeting HMGB1.

PURPOSE: To investigate the effects of microRNAs-107 (miR-107) on autophagy, proliferation, and migration of breast cancer cells and its mechanism by targeting high mobility group protein B1 (HMGB1). METHODS: Real-time polymerase chain reaction assay was used to detect the expression of miR-107 in breast cancer and its cell lines. In MDA-MB-231 and MDA-MB-453 breast cancer cells, the expression of p62, Beclin1 protein, and the changes of cell proliferation and migration after overexpression of m miR-107 were detected by Western blotting, 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide, and transwell assays. Target Scan online prediction, dual luciferase reporter gene, and Western blot were used to verify the targeting relationship between miR-107 and HMGB1. The effects of silencing HMGB1 expression on p62, Beclin1 protein expression, cell proliferation, and migration ability were detected. The transfected MDA-MB-453 cells were inoculated into the right axilla of the nude mice, the tumor volume and weight were weighed, and the expression of miR-107, HMGB1, p62, and Beclin1 in the tumor were detected. RESULTS: The expression of miR-107 was downregulated in breast cancer tissues and cell lines (P < 0.01). The expression of p62 protein was upregulated (P < 0.01), while Beclin1 protein was downregulated (P < 0.01) and cell proliferation and migration ability were decreased (P < 0.01) after overexpressing miR-107 in MDA-MB-231 and MDA-MB-453 cells. The results of TargetScan online prediction, dual luciferase reporter gene, and Western blot showed that miR-107 could regulate HMGB1 expression. The expression of p62 protein was upregulated (P < 0.01), while Beclin1 protein was downregulated (P < 0.01) and cell proliferation and migration ability were decreased (P < 0.01) after silencing HMGB1 in MDA-MB-231 and MDA-MB-453 cells. The results of xenograft experiments showed that miR-107 could delay tumor growth and inhibit autophagy. CONCLUSION: miR-107 could inhibit cell autophagy, proliferation, and migration of breast cancer cells by targeting HMGB1.

Anti-tumoral activity of native compound morelloflavone in glioma.

The aim of the study was to investigate the anti-tumoral activity of morelloflavone substances with different structures. We also studied the possible link between morelloflavone structure and its function. Various types of chromatographic techniques were used to isolate and screen morelloflavone substances from the extracts of gambogic tree trunk and the morelloflavone structures were identified by analytical techniques such as high resolution mass spectrometry and nuclear magnetism. Anti-tumoral activities of different compounds were investigated and the link between the antitumor activity and the structure of compound was exaimed. Our results showed that the isolated morelloflavone substances demonstrated a certain level of antitumor activity. The compound no. 1 had the strongest effect to inhibit glioma U87 and C6 cells followed by compound no. 2 while compound no. 5 was the weakest among them. We conducted a preliminary analysis on the structure-function relationship through the structure comparison and we concluded that the antitumor effects of morelloflavone substances with different structures were significantly different from each other. Thus, the glucose chain in C4 position of biflavone structure can enhance the antitumor activity of the compound in glioma cells. Additionally, the formation of intramolecular hydrogen bonds in biflavone compounds may also play a role in enhancing the antitumor activity and inhibition rate.