Evaluating the potential for utilising migratory locust powder (Locusta migratoria) as an alternative protein source in peanut-based ready-to-use therapeutic foods.

This study aimed at exploring the possibility of substituting powdered milk (PM) with migratory locust powder (MLP) as a cheap and sustainable alternative protein source in peanut-based ready-to-use therapeutic foods used in treatment of malnutrition. The migratory locust is a wide spread and underutilised high protein edible insect. Peanut-based ready-to-use therapeutic foods (RUTFs) were formulated according to the MSF/UNICEF 2013 nutritional standards. Milk powder was substituted with MLP at 5-30% levels. RUTF containing 30% milk powder was used as the control. Nutritional, antioxidant and antinutrients composition of the insect-enriched RUTFs were compared with RUTF containing milk powder only and a standard. The protein content of the insect-enriched RUTFs ranged from 19.58-26.38% exceeding the MSF/UNICEF minimum standard for protein. RUTF with 10% PM and 20% MLP had the highest (p < 0.05) mineral contents. All formulations had more thiamine (vitamin B1) than the recommended level of 0.5 mg/100 g. Niacin (Vitamin B3) was the most abundant vitamin in the formulations with considerable amounts of vitamin D and E. All MLP-enriched RUTFs met the FAO 2011 standard requirements for amino acids for children aged six months to three years. Therapeutic food with 30% MLP had the highest radical scavenging ability against DPPH, and iron chelating activity. Levels of phytate and tannin were below the tolerable limits. Therefore, MLP can be used as a low-cost substitute for milk powder in producing ready-to-use therapeutic foods.

Assessment of Mulberry Silkworm Pupae and African Palm Weevil larvae as alternative protein sources in snack fillings.

The search for alternative food sources especially protein to meet the nutritional demand of the projected 9 billion world population by 2050 is now critical. Edible insect is an alternative source of protein in many African and Asian cuisines where beef, pork and chicken are perceived to be relatively expensive. The current study evaluates Mulberry Silkworm Pupae (MSP) and African Palm Weevil larvae (APW) as substitute to the mainstream proteins in snacks fillings, and also assessing the consumer acceptability of the new products. The chemical composition showed that MSP is higher in protein and soluble fibre contents while APW is higher in crude fat, crude fibre, zinc, manganese and calcium contents. The cooked edible insects were rich in both essential and non-essential amino acids. When used as fillings for snacks, the protein content of the snacks produced with APW and MSP compared favourably well with the snacks produced with beef fillings. The fat contents of the snacks were 18 % lower than those of snacks made with beef fillings. The mineral contents of the snack with APW were significantly higher than the other samples. There was no significant difference in the taste and overall acceptability of samosa snack produced with beef, APW and MSP. African palm weevil larvae and Mulberry silkworm pupae could serve as alternative sources of protein in the production of snacks and cuisines, and a viable source of income generation.

Inclusion of Baobab (Adansonia digitata L.) Fruit Powder Enhances the Mineral Composition and Antioxidative Potential of Processed Tigernut (Cyperus esculentus) Beverages.

The functional beverage market has recently increased due to the health benefits in addition to their nutritional and thirst-quenching functions. Tigernut is an economic crop with reported health benefits. This study evaluates the antioxidative potential of processed tigernut extracts fortified with baobab fruit pulp powder. The ferric reducing antioxidant potential, 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid), and 1,1-diphenyl-2-picrylhydrazyl were used to determine the antioxidant capacity, while the Folin-Ciocalteu method was used to measure the total phenolic content of the beverages. The inclusion of baobab fruit significantly (P<0.05) increased the total phenolic content by 18% (from 31.06 to 36.83 mg gallic acid equivalent/100 mL) and the flavonoid content by 15∼20%, while the vitamin C content peaked at 39.6 mg/100 mL. There was a significant correlation between the phenolic and vitamin C contents. Overall, the antioxidant potentials were elevated with the inclusion of baobab powder. All the beverages included in this study are good sources of Ca, P, and K; the contents of Ca, P, and K in the roasted tuber extracts with baobab peaked at 210.91, 8.70, and 93.35 µg/mL, respectively. However, the K:Na ratio was greater than 5:1. Although, baobab increased the acidity of the beverages, it did not significantly diminish the consumer acceptability, with the values ranging from 7.62 to 8.40 on a 9-point Hedonic scale. The beverages have potential for use as natural antioxidants and could be recommended for consumers with diets deficient in Ca and K, particularly in food insecure communities. They could also be used as a replacement for sugar-sweetened carbonated beverages.

Occurrence of bacteria and endotoxins in fermented foods and beverages from Nigeria and South Africa.

In Africa, fermented foods and beverages play significant roles in contributing to food security. Endotoxins are ubiquitous heat stable lipopolysaccharide (LPS) complexes situated in the outer cell membranes of Gram-negative bacteria. This study evaluated the microbiological quality of fermented foods (ogiri, ugba, iru, ogi and ogi baba) and beverages (mahewu and umqombothi) from selected Nigerian and South African markets. The bacterial diversity of the fermented foods was also investigated and the identity of the isolates confirmed by biochemical and molecular methods. Isolate grouping was established through hierarchal clustering and the samples were further investigated for endotoxin production with the chromogenic Limulus Amoebocyte Lysate assay. The total aerobic count of the samples ranged from 5.7 to 10.8 Log CFU/g. Fourteen bacteria genera were detected with most of the isolates being members of the Enterobacteriaceae family. Sphingomonas paucimobilis and Escherichia coli were the dominant Gram-negative bacterial species detected. There were considerable variations in the concentrations of endotoxins produced and the lowest endotoxin concentration was found in ogi (4.3 × 10 EU/g) and the highest in iru (5.5 × 104 EU/g) while, 44% of umqombothi samples had endotoxins. Ogi baba samples had better microbial quality than other samples due to its reduced bacterial load and endotoxin levels. There was a strong positive (r = 0.714, r = 0.996) and significant (p < 0.01) correlation between the endotoxin levels and bacterial loads of the samples. Some previously unreported species of bacteria found in the fermented foods included Aeromonas haemolyticus and Rhizobium radiobacter. This is the first comprehensive report on endotoxins in fermented foods and beverages in Africa. Furthermore, the occurrence of pathogenic bacteria and toxins in the foods and beverages is of serious concern that calls for immediate action.

Optimization of extrusion conditions for the production of instant grain amaranth-based porridge flour.

Malnutrition is one of the foremost causes of death among children below 5 years in developing countries. Development of nutrient-dense food formulations using locally available crops has been proposed as a means to combat this menace. This study optimized the extrusion process for the production of a nutritious amaranth-based porridge flour. Least cost formulations containing grain amaranth, groundnut, iron-rich beans, pumpkin, orange-fleshed sweet potato, carrot, and maize were developed and evaluated by a sensory panel (n = 30) for acceptability using the 9-point hedonic scale. Extrusion process of the most acceptable porridge flour was optimized by response surface methodology (RSM). Barrel temperature (130-170°C) and feed moisture content (14%-20%) were the independent variables which significantly (p < .05) affected in vitro protein digestibility, vitamin A retention, total polyphenol, phytic content, and iron and zinc extractabilities. Optimization of the extrusion process improved the nutritional quality of the instant flour.