Replacing alfalfa-based total mixed ration with Moringa leaves for improving carcass and meat quality characteristics in lambs.

The expected increase in the population will put more pressure on resources, which means there will be a greater demand for food and the need for different ingredients to feed animals. This study aimed to explore the potential benefits of replacing alfalfa-based total mixed ration (TMR) with Moringa spp. On carcass characteristics and meat quality traits in lamb. The experiment involved 60 intact male lambs of the Harri breed, averaging 24 ± 1 kg in weight and approximately 5 months old. Lambs were randomly divided into five feeding groups: T1 (40 % alfalfa-based TMR), T2 (40 % M. oleifera), T3 (40 % M. peregrina), T4 (20 % alfalfa-based TMR + 20 % M. oleifera), and T5 (20 % alfalfa-based TMR + 20 % M. peregrina. The results confirmed that incorporating Moringa spp. Into lamb diets significantly reduced intramuscular fat content. Supplementation also improved tenderness, texture properties, and water-holding capacity. Additionally, the treatment groups exhibited a significant decrease in total non-carcass components compared to the control group. Overall, these findings suggest positive changes and enhancements in lamb carcass traits and meat quality attributes with Moringa spp. Supplementation, without observed adverse effects.

Assessing the Influence of Guanidinoacetic Acid on Growth Performance, Body Temperature, Blood Metabolites, and Intestinal Morphometry in Broilers: A Comparative Sex-Based Experiment.

It is well known that female and male broilers showcase variations in their growth performance, influenced by various physiological factors. This experiment aims to explore potential differences between female and male broilers concerning growth performance, body temperature, blood metabolites, carcass traits, and intestinal architecture in response to guanidinoacetic acid (GAA) supplementation. A total of 240 Ross 308 broiler chickens were arranged in a 3 × 2 factorial design and randomly allocated into 48 boxes, each containing 5 birds. The experiment comprised six treatments, with eight replicates per treatment. The main factors investigated were dietary GAA levels (0%, 0.06%, and 0.12%) and sex (male and female). Male broilers demonstrated superior body weight gain (BWG) and feed intake (FI) compared to females (p< 0.05). GAA supplementation at 0.12% concentration notably improved BWG and reduced FI and feed conversion ratio (FCR) across experimental phases (p < 0.05). However, interactions between sex and GAA were minimal except for reduced FI and FCR (p < 0.05) in both sexes during early growth stages. Regardless of GAA treatment, the male birds exhibited more elevated shank and head temperatures than the females. Carcass traits were largely unaffected by GAA supplementation or sex, except for higher heart yield in the males. Serum metabolite levels were not different between treatment groups at 10 and 24 days of age, except for a higher level of serum creatinine at 10 days in the female birds with 0.06% GA supplementation (p < 0.05). Intestinal morphology was significantly affected by GAA and sex, depending on the segment of intestine, in which GAA supplementation significantly increased villus height, crypt depth, villus width, surface area, and goblet cell count, while the males consistently exhibited higher values of these parameters than the females, and differences were observed between intestinal segments, especially in the ileum and duodenum, at different ages. In conclusion, the interactions between GAA and sex had minimal influences on growth performance indices. However, male broilers demonstrated a more pronounced response to GAA concerning ileal architecture. This study highlights the importance of supplementing broiler chicken diets with GAA for optimizing male broiler performance and intestinal function. The inclusion of GAA into broiler diets needs further study to reveal the underlying mechanisms driving these sex-specific responses and assess the long-term impacts of GAA supplementation on broiler health and productivity.

Proposing a strategy based on body-thermal status to improve the welfare of heat-stressed and water-deprived goats (Capra hircus).

Objective: Despite the considerable body of research on the effects of heat stress coupled with water scarcity (either through restriction or deprivation) on goats, aimed at enhancing their welfare, there remains a notable gap in the literature regarding the subsequent period following water restoration, during which the cumulative impact is fully alleviated. In response to this gap, we propose a strategy grounded in the assessment of body-thermal status to improve the welfare of heat-stressed and water-deprived goats. Specifically, our strategy seeks to determine the minimally required recovery interval necessary to completely mitigate the residual effects of water deprivation endured for a duration of 72 hours. Methods: Eight healthy Aardi bucks, aged 10 months and weighing 30 kg, were subjected to three distinct stages: euhydration, dehydration, and rehydration. Each stage spanned for 72 hours except for the rehydration stage, which was left unrestricted. Various meteorological, biophysiological, and thermophysiological measurements were subsequently recorded. Results: Exposure of heat-stressed goats, as indicated by the temperature-humidity index values, to a 72 hours deprivation period resulted in noticeable (p<0.05) alterations in their biophysiological (daily feed intake, body weight, and feces water content) and thermophysiological responses (core, rectal, skin, and surface temperatures, respiratory and heart rates, internal, external, and total body-thermal gradients, heat tolerance and adaptability coefficients, heterothermial total body-heat storage, and total water conservation). Remarkably, our findings demonstrate that all assessed variables, whether measured or estimated, returned to their baseline euhydration levels within 10 days of commencing the rehydration phase. Conclusion: In order to improve the welfare of heat-stressed and 72 hours water-deprived goats, it is imperative to allow a recovery period of no less than 10 days following the restoration of water access prior to initiating any subsequent experiments involving these animals. Such experiments, addressing these critical aspects, serve to advance our understanding of goat welfare and obviously hold promise for contributing to future food security and economic viability.

Investigating the impact of preslaughter handling intensity on goats: a study on behavior, physiology, blood enzymes, and hormonal responses.

Introduction: The present study evaluated the effect of preslaughter stress intensities on the behavioral, physiological, blood biochemicals, and hormonal responses in goats. Methods: Twenty-seven intact male goats (Ardi breed, 10 months of age, 27 kg liveweight) were divided into three treatment groups viz., the control (C) group, ear pulling (EP) group, and leg pulling (LP) group. Various behavioral, physiological, blood biochemical and hormonal responses were recorded before and after handling. Results and Discussion: The EP and LP goats had a higher frequency and intensity of vocalization as compared to control goats. The preslaughter handling stress intensities had a significant effect on the before and after handling values of heart rate, respiration rate, rectal temperature, and ear temperature. Further, among groups, the glucose value increased significantly upon preslaughter handling as compared to the baseline value. The LP goats had significantly higher after-handling value for lactate dehydrogenase (LDH) as compared to the before-handling value. The catecholamines (adrenaline and noradrenaline) and ß-endorphin concentrations increased significantly upon preslaughter handling. The higher physiological, behavioral, blood biochemical, and hormonal response indicated higher preslaughter stress in EP and LP goats. Both levels of intensity revealed unfavorable responses in goats that may adversely affect animal welfare and meat quality. Thus, to ensure minimal adverse effects on behavior, physiology, blood enzymes, and hormonal responses, it is recommended to follow animal welfare principles when implementing preslaughter handling practices.

Applicability of thermography as a potential non-invasive technique to assess the body-thermal status of heat-stressed and water-deprived goats (Capra hircus).

To evaluate the use of infrared thermography to assess the thermal status of heat-stressed and water-deprived Capra hircus, full-body surface temperature (TS) and six other body-thermal variables [core, rectal (TR), and skin (TSK) temperatures, respiratory and heart rates, and total body-thermal gradient (core-to-ambient, BTG)] were measured after three days of euhydration (EU), dehydration (DE), and rehydration (RE). Results revealed that the combined effect of heat stress and water deprivation had affected all tested variables including the TS, and once these animals gained access to water in the RE stage variables returned to their EU levels. Moreover, there were positive correlations between TS and all variables with the exception of BTG. From these six variables, only three variables (i.e. the TR, TSK, and BTG) during the DE stage and two variables (i.e. the TSK and BTG) throughout the experimental stages showed higher constancy (R2 ≥ 0 75, P < 0 001; agreement intervals ±1 96 95 % CI) with TS. However, BTG appeared more closely correlated with TS, representing the body-thermal status more realistically than other variables. In effect, the mean and thresholds of the BTG were predicted using the recorded TS and were within 0.02 °C of original estimates. Collectively, these findings show that infrared thermography is appropriate for assessing body-thermal status, and thus the welfare, of these animals under the three conditions studied, and conclude that full-body TS can be a surrogate proxy for BTG in these animals. Further experiments are needed to adequately examine the reproducibility of these results under biometeorologically-simulated environments and natural habitats.

Investigating the Chemical Composition of Lepidium sativum Seeds and Their Ability to Safeguard against Monosodium Glutamate-Induced Hepatic Dysfunction.

Monosodium glutamate (MSG) is one of the most frequently used food additives that endanger public health. The antioxidant, hyperlipidemic, and cytoprotective properties of Lepidium sativum seeds (LSS) as a natural remedy can minimize the harmful effects of MSG. This study investigated the potential protective effect of LSS against MSG-induced hepatotoxicity in rats. Male albino Sprague Dawley rats (n = 24) were equally divided into four groups for 30 days: the control group (G1) received a basal diet without supplement, group (G2) was fed a basal diet + MSG (30 g/kg b.w.) as a model group, group (G3) was fed a basal diet + MSG (30 g/kg b.w.) + LSS (30 g/kg b.w.), and group (G4) was fed a basal diet + MSG (30 g/kg b.w.) + LSS (60 g/kg b.w.). LSS enhanced serum alkaline phosphatase activity as well as total cholesterol, triglyceride, and glucose levels. It can decrease peroxide content in serum lipids and inhibit glutathione reductase and superoxide dismutase in hepatic cells. The dietary supplementation with LSS provided cytoprotection by enhancing the histoarchitecture of the liver and decreasing the number of apoptotic cells. Due to their antioxidant and anti-apoptotic properties, LSS effectively protect against the hepatotoxicity of MSG. These findings are of the highest significance for drawing attention to incorporating LSS in our food industry and as a health treatment in traditional medicine to combat MSG-induced hepatic abnormalities.

Effect of dietary supplementation of betaine and organic minerals on growth performance, serum biochemical parameters, nutrients digestibility, and growth-related genes in broilers under heat stress.

Global warming and climate changes have a detrimental impact on poultry production, causing substantial economic losses. This study investigated the effects of incorporating dietary betaine (BT) and organic minerals (OMs) on broilers' performance as well as their potential to mitigate the negative impacts of heat stress (HS). Six hundred 1-day-old Ross 308 chicks were randomly allocated to 12 experimental treatments with 5 replicates of 10 birds each (5 male + 5 female). The birds were provided with diets containing BT (0 and 2,000 ppm) and OMs (0, 250, and 500 ppm), either individually or in combination, under both thermoneutral and HS-inducing temperatures. The HS conditions involved exposing the birds to cyclic periods of elevated temperature (35°C ± 2°C) for 6 h daily, from 10:00 am to 4:00 pm, starting from d 10 and continuing until d 35. The exposure to HS deteriorated birds' growth performance; however, dietary BT and OMs inclusion improved the growth performance parameters bringing them close to normal levels. Carcass traits were not affected by dietary supplementation of BT, OMs, HS, or their interaction. Interestingly, while HS led to increased (P < 0.05) levels of total cholesterol, LDL-cholesterol, and hepatic malondialdehyde (MDA), these adverse effects were mitigated (P < 0.05) by the addition of BT and OMs. Moreover, dietary BT supplementation led to elevated serum total protein and globulin concentrations. Cyclic HS did not alter Mn, Zn, and Cu contents in the pectoral muscle. However, the incorporation of OMs at both levels increased concentrations of these minerals. Notably, the combination of 500 ppm OMs and 2,000 ppm BT improved Mn, Zn, Cu, and Fe digestibility, which has been compromised under HS conditions. Cyclic HS upregulated gene expression of interleukin-1ß, heat shock protein 70, and Toll-like receptor-4 while downregulated the expression of claudin-1, uncoupling protein, growth hormone receptor, superoxide dismutase 1, glutathione peroxidase 1 and insulin-like growth factor 1. The aforementioned gene expressions were reversed by the combination of higher dietary levels of BT and OMs. In conclusion, the dietary supplementation of 500 ppm OMs along with 2,000 ppm BT yielded significant improvements in growth performance and mineral digestibility among broiler chickens, regardless of thermal conditions. Moreover, this combination effectively restored the expression of growth-related genes even under heat-stress conditions.

Effects of increasing levels of lasalocid supplementation on growth performance, serum biochemistry, ruminal fermentation profile, in vitro nutrient digestibility, and gas production of growing goats.

Introduction: Lasalocid is a feed additive widely used in ruminant nutrition and plays a crucial role in improving livestock productivity, digestibility, immunity, and overall wellbeing. The current study was conducted to investigate the effect of different levels of lasalocid (LAS) supplementation on growth performance, serum biochemistry, ruminal fermentation profile, in vitro nutrient digestibility, and gas production of growing goats. Methods: A total of 60 growing Aardi male goats with an average body weight of ~17.12 kg (3-month-old) were used for an 84-day trial. Animals were randomly divided into four treatment groups with 5 replicates of 3 goats each. All four groups were provided with a basal diet supplemented with lasalocid (LAS) at 0 (without supplementation; LAS0), 10 (LAS10), 20 (LAS20), or 30 (LAS30) ppm LAS/kg dry matter (DM). Feed intake was measured weekly, and goats were weighed every 2 weeks for an evaluation of the performance parameters. Blood samples were collected for the measurement of biochemical variables. In vitro nutrient digestibility and gas production were evaluated. Results and discussion: The supplementation of LAS at level 30 ppm/kg DM increased (P < 0.05) the body weight gain and average daily gain without linear or quadratic effect. The serum concentrations of high-density lipoprotein were significantly (P < 0.05) higher in the LAS20 group than in other groups with linear and quadratic effects, while low-density lipoprotein concentration was significantly lower in the LAS20 group than in LAS0 and LAS30 with a linear effect. Different levels of lasalocid supplementation had no effect on the ruminal fermentation profile, in vitro gas production, and nutrient digestibility. In conclusion, the addition of LAS (20-30 ppm/kg DM) to the goat's diet can improve the growth performance and lipoprotein profile.

Evaluation of the Nutritive Value and Digestibility of Sprouted Barley as Feed for Growing Lambs: In Vivo and In Vitro Studies.

The main objective of this study was to investigate the effects of freshly sprouted barley on the growth of lambs, in addition to its nutritional value and digestibility. In addition, sprouted barley digestibility and rumen fermentation were studied in vitro on a dry matter (DM) basis. A total of 45 three-month-old Awassi lambs were randomly assigned to five treatments of sprouted barley (0, 25, 50, 75, 100%) diets. Bodyweight, weight gain, feed intake and feed efficiency were recorded every two weeks. Nutrient analyses were performed on feed, faecal, and urine samples. DM and non-fibrous carbohydrates were measured. Digestibility of DM, organic matter (OM), and neutral detergent fiber (NDF), as well as gas production, pH value, ammonia-N, and volatile fatty acids (VFAs), were determined in vitro using continuous culture. The results showed that final bodyweight was lower (p < 0.05), while feed intake and the feed-to-gain ratio were increased (p < 0.05) in sprouted barley treatments. Nutrient analysis indicators of sprouted barley treatments (25 to100%) were lower (p < 0.05) for DM, crude protein, acid detergent fiber, lignin and ash, and higher for total digestible nutrients, NDF, fat, phosphorus, zinc, copper, and net energy than the traditional diet. In the in vivo study, the digestibility of nutrients in sprouted barley treatments was improved (p < 0.05), while the diet (sprouted barley 100%) had the lowest digestibility of DM, OM, and NDF compared with the other treatments in the in vitro study. In conclusion, the addition of sprouted barley improved digestibility, and fermentation characteristics, while having a negative effect on growth. Further studies are recommended for optimal growth performance.

Effects of different levels of spirulina (Arthrospira platensis) supplementation on productive performance, nutrient digestibility, blood metabolites, and meat quality of growing Najdi lambs.

In a 90-day study, 32 growing lambs aged 3 months were utilized to evaluate the effects of various levels of spirulina dietary supplement on productive performance, nutritional digestibility, and meat quality in growing Najdi lambs. The lambs were put into 4 groups of 8 lambs each at random. The diet consisted of a total mixed ration (TMR) without spirulina (CONT), and the TMR diet supplemented with spirulina at the levels of 2 ppm (SPP2), 4 ppm (SPP4), and 8 ppm (SPP8). The treatment groups, especially SPP8, showed a significant (p < 0.05) increase in body weight and average daily gain (p < 0.05) compared to the CONT group. Dry matter intake and acid detergent fiber were also significantly (p ≤ 0.05) higher in SPP8 compared to other treated groups and CONT. The N absorption, N retention, and percentage of N digestibility coefficient were greater (P < 0.05) in lambs in the treatment groups than in the CONT. Blood biochemistry variables were not significantly (p ≤ 0.05) affected by the treatments, with the exception of the serum concentration of triglyceride and bilirubin. Carcass profile and meat quality, including back fat, body wall fat, and cooking loss, were increased significantly (p ≤ 0.05) with dietary spirulina. From the results of the present study, it was concluded that spirulina dietary supplementation at the level of 8 ppm increased weight gain, nutritional digestibility, nitrogen utilization, and meat composition in growing Najdi lambs.

Using chrono-physiological management in form of shifting the feeding time has no advantage in goat kids exposed to experimentally induced heat stress.

Despite the proven outcomes of implementing chrono-physiological management (CPM) in several animals to promote their production performance, studies on its usefulness in goats are lacking. Twelve healthy Aardi male goat kids (22.60±1.10kg body weight; 6 months of age) were randomly allocated into two groups (morning-fed at 09:00 h and evening-fed at 21:00 h). The physiological and productive advantage of CPM (in form of shifting feeding time) was investigated in both groups while exposed to experimentally induced heat stress for 42 days (daily ambient temperature was cycling from 25 to 45°C). Thermophysiological (rectal, skin, and coat temperatures as well as respiratory rate), blood biochemical (plasma level of albumin, glucose, triacylglycerol, and urea), and production performance (daily feed intake, average daily gain, and feed conversion ratio) measurements were all obtained throughout this period. Current findings collectively revealed that shifting feeding time from 09:00 to 21:00 h had no (P > 0.05) impact on the thermophysiological status and blood metabolites of heat stressed kids. Above all, evidences have indicated that shifting feeding time had failed (P > 0.05) to demonstrate any consequence on promoting growth in these kids, thereby suggesting that shifting feeding time under hot climatic conditions has no advantage in goats. In fact, implementation of such an approach might not to be encouraged for heat-adapted animals. Experiments using other CPM methods (such as simultaneous shift of both feeding time and lighting program) can be however of further interest.

Identifying potential thermal drivers of sudomotor in camels (Camelus dromedarius).

The mechanism of sudomotor regulation in the family Camelidae, as in other mammals, is poorly understood. Five healthy dromedary bulls (400 kg and 4 years-old) were used to examine the interrelationship of sweating rate (SR) with ten thermal parameters measured (and/or estimated) every 3-hr for a 24-hr time period under natural and shaded environmental conditions, in order to subsequently identify the potential thermal drivers of sudomotor in this species. Results revealed that all parameters, including SR, had clearly (P < 0·001) exhibited monophasic circadian rhythms. Moreover, the obtained findings pointed out that strong/moderate positive correlations were existed between SR and eight parameters [i.e. ambient (Ta), rectal (Tr), body (Tb), skin (Tsk), coat (Tct) temperatures as well as total (ambient-to-body, BTGt), external (ambient-to-skin, BTGex), and internal (skin-to-body, BTGin) body thermal gradients] suggesting that they may all be good indicators of sweating activity. Nonetheless, out of those highly-correlated parameters, merely six (i.e. Ta, Tb, Tsk, Tct, BTGt, and BTGex) showed superior coefficients of determination (R2 ≥ 0·90; P < 0·000) when interrelated with SR; thereby, implying that they have the potential to drive sudomotor. Notably, however, results were more probably allude that sudomotor is regulated through BTGt. Accordingly, the onset of sweating (i.e. threshold) and its effective level was determined using BTGt. A method of how SR can be regulated through BTGt was proposed according to Webb's theory of controlling body-heat content. Some shortcomings prevent confirming that BTGt is the best thermal driver of sudomotor in this species were noted. Research dealing with this interesting physiological process requires further experimentation to fully elucidate the basic functional mechanisms of Camelidae's thermoregulatory system .