Co-application of copper oxide nanoparticles and Trichoderma harzianum with physiological, enzymatic and ultrastructural responses for the mitigation of salt stress.

Chemical contamination or nutrient pollution is concerning for health, environmental, and economic reasons. Ecofriendly surface modification of nanoparticles is a consistent challenge for agricultural purposes. In response to this environmental concern, CuO-NPs synthesized through biological method using green source and characterized for morphological and structural features through SEM (scanning electron microscope) and TEM (transmission electron microscope) spectroscopy. Our research findings illustrate that the presence of salt stress induces a notable decline in both physiological and biochemical parameters within plants. Nevertheless, the utilization of T. harzianum and CuO-NPs exhibited a mitigating effect on the detrimental consequences induced by salt stress in plants. The application of T. harzianum and the simultaneous co-inoculation with CuO-NPs notably enhanced fresh biomass and facilitated vegetative growth in comparison to the control group. Furthermore, the exposure of both T. harzianum inoculum and Copper oxide nanoparticles resulted in a significant reduction of oxidative stresses, including reactive oxygen species (ROS) levels, H2O2, and lipid peroxidation (MDA) levels in the above-ground parts of the plant, while also minimizing electrolyte leakage (EL) by reducing root growth. Additionally, the co-inoculation of the endophyte and CuO-NPs led to a significant enhancement in antioxidant enzymatic activities, such as superoxide dismutase (SOD) and chitinase (CAT) activity in the above-ground parts, under salt stress conditions. The inoculum, along with its combination with CuO-NPs, decreased electrolyte conductivity and improved total chlorophyll contents as compared to the control. The combined application of T. harzianum and CuO-NPs improved salt tolerance in A. thaliana plants by triggering salt-associated gene expression. These findings suggest that the application of T. harzianum and CuO-NPs can considerably promote leaf anatomical changes in A. thaliana and have ability to enhance salt tolerance, particularly in saline areas.

Groundwater contamination with the threat of COVID-19: Insights into CSR theory of Carroll's pyramid.

In this study, we elucidated the effect of sewage drain on groundwater contamination as including different contaminants, microbes, and pathogens, which deteriorating the groundwater by poor infiltration and seepage. This is getting severer in developing countries like India, Bangladesh, and Pakistan, where unprocessed effluent is discharged into the water bodies. This study was planned to elucidate the effect of sewage drain (based on distance 0-5, 5-10, 15-20, 20-25 m) from two different sewage drains to explain the different physiochemical, and biological parameters including total soluble solids (TSS), chloride, total dissolved solids (TDS), calcium, total hardness, magnesium, nitrate, chemical oxygen demand (COD), dissolved oxygen (D.O.), and biological oxygen demand (BOD). Drainage channel number-1 results showed that E. coli (positive), coliform count (22.75-48.66 /100 mL), and BOD (8-25.75 mgL-1) remained above the permissible limit of the World Health Organization (WHO). Besides, drainage channel number 2 results exposed that E. coli (positive), coliform count (17.7-47 /100 mL), and BOD (6.25-21.5 mg/ L) was not within the permissible limit of WHO. The presence of COVID-19 in the stool has been significantly reported in the literature. The presence of stool in sewage drain leading to groundwater contamination can be an emerging threat to water pollution and could lead to the spread of COVID-19. This study helps to minimize this threat with the help of corporate social responsibility (CSR). Because organizational responsibility towards its society is one of the critical factors to contain numerous issues related to the society.