RESUMO
BACKGROUND: Influenza attacks the epithelium of the lung, causing cell death and disruption of the epithelial barrier leading to fluid buildup in the lung and impairment of gas exchange. Limited treatment options for severe influenza pneumonia prioritize the need for the discovery of effective therapies. IL-22 is a cytokine that promotes tissue integrity and has strong promise as a treatment option. While research has been focused on the cytokine itself, there is limited understanding of the regulation of the IL-22 receptor (IL-22Ra1) at the epithelial surface during infection. METHODS: IL-22Ra1 levels were measured by qRT-PCR, western blot and immunofluorescence following H1N1 influenza infection (A/PR/8/34 H1N1) or synthetic TLR3 mimetic, Poly (I:C). Regulation of the receptor was determined using STAT inhibitors (STAT1, STAT3 and PanSTAT inhibitors), TLR3 inhibition, and neutralization of interferon alpha receptor 2 (IFNAR2). Significance was determined by a p-value of greater than 0.05. Significance between two groups was measured using unpaired t-test and significance between more than two groups was measured using one-way ANOVA with Tukey Multiple Comparison Test. RESULTS: Here we show both in vivo and in vitro that IL-22Ra1 was induced as early as 24 h after influenza (H1N1 PR8) infection. This induction was triggered by toll-like receptor 3 (TLR3) as a TLR3 mimetic [Poly (I:C)] also induced IL-22Ra1 and inhibition of endosomal formation required for TLR3 function inhibited this process. This upregulation was dependent upon IFNß signaling through STAT1. Importantly, induction of IL-22Ra1 significantly increased IL-22 signaling as evidenced by pSTAT3 levels following IL-22 treatment. CONCLUSION: Collectively, these data suggest epithelial cells may optimize the beneficial effects of IL-22 through the induction of the IL-22 receptor during viral infection in the lung.
Assuntos
Influenza Humana/metabolismo , Receptores de Interleucina/biossíntese , Fator de Transcrição STAT1/biossíntese , Receptor 3 Toll-Like/biossíntese , Células A549 , Animais , Cloroquina/farmacologia , Humanos , Influenza Humana/patologia , Interferons/farmacologia , Masculino , Camundongos , Camundongos Endogâmicos C57BL , Poli I-C/farmacologiaRESUMO
Influenza A is a highly contagious respiratory virus that causes seasonal epidemics and occasional worldwide pandemics. The primary cause of influenza-related mortality is bacterial superinfection. There are numerous mechanisms by which preceding influenza infection attenuates host defense, allowing for increased susceptibility to bacterial pneumonia. Herein, we demonstrate that influenza inhibits Staphylococcus aureus-induced production of interleukin-33 (IL-33). Restoration of IL-33 during influenza A and methicillin-resistant S. aureus superinfection enhanced bacterial clearance and improved mortality. Innate lymphoid Type 2 cells and alternatively activated macrophages are not required for IL-33-mediated protection during superinfection. We show that IL-33 treatment resulted in neutrophil recruitment to the lung, associated with improved bacterial clearance. These findings identify a novel role for IL-33 in antibacterial host defense at the mucosal barrier.
Assuntos
Vírus da Influenza A/imunologia , Interleucina-33/metabolismo , Linfócitos/imunologia , Infecções por Orthomyxoviridae/imunologia , Mucosa Respiratória/imunologia , Infecções Estafilocócicas/imunologia , Staphylococcus aureus/imunologia , Animais , Carga Bacteriana , Imunidade Inata , Interleucina-33/genética , Masculino , Camundongos , Camundongos Endogâmicos C57BL , Camundongos Knockout , Infiltração de Neutrófilos , Mucosa Respiratória/microbiologia , Mucosa Respiratória/virologia , Superinfecção , Células Th2/imunologiaRESUMO
Approximately 2 billion people are infected with Mycobacterium tuberculosis (Mtb), resulting in 1.4 million deaths every year. Among Mtb-infected individuals, clinical isolates belonging to the W-Beijing lineage are increasingly prevalent, associated with drug resistance, and cause severe disease immunopathology in animal models. Therefore, it is exceedingly important to identify the immune mechanisms that mediate protection against rapidly emerging Mtb strains, such as W-Beijing lineage. IL-22 is a member of the IL-10 family of cytokines with both protective and pathological functions at mucosal surfaces. Thus far, collective data show that IL-22 deficient mice are not more susceptible to aerosolized infection with less virulent Mtb strains. Thus, in this study we addressed the functional role for the IL-22 pathway in immunity to emerging Mtb isolates, using W-Beijing lineage member, Mtb HN878 as a prototype. We show that Mtb HN878 stimulates IL-22 production in TLR2 dependent manner and IL-22 mediates protective immunity during chronic stages of Mtb HN878 infection in mice. Interestingly, IL-22-dependent pathways in both epithelial cells and macrophages mediate protective mechanisms for Mtb HN878 control. Thus, our results project a new protective role for IL-22 in emerging Mtb infections.
Assuntos
Células Epiteliais/imunologia , Interleucinas/metabolismo , Pulmão/imunologia , Macrófagos/imunologia , Mycobacterium tuberculosis/imunologia , Tuberculose/imunologia , Animais , Células Cultivadas , Doença Crônica , Resistência a Medicamentos , Humanos , Imunidade nas Mucosas , Interleucinas/genética , Pulmão/microbiologia , Pulmão/patologia , Macaca mulatta , Camundongos , Camundongos Endogâmicos C57BL , Camundongos Knockout , Receptor 2 Toll-Like/genética , Receptor 2 Toll-Like/metabolismo , Interleucina 22RESUMO
Bacterial pneumonia and tracheobronchitis are diagnosed frequently following lung transplantation. The diseases share clinical signs of inflammation and are often difficult to differentiate based on culture results. Microbiome and host immune-response signatures that distinguish between pneumonia and tracheobronchitis are undefined. Using a retrospective study design, we selected 49 bronchoalveolar lavage fluid samples from 16 lung transplant recipients associated with pneumonia (n = 8), tracheobronchitis (n = 12) or colonization without respiratory infection (n = 29). We ensured an even distribution of Pseudomonas aeruginosa or Staphylococcus aureus culture-positive samples across the groups. Bayesian regression analysis identified non-culture-based signatures comprising 16S ribosomal RNA microbiome profiles, cytokine levels and clinical variables that characterized the three diagnoses. Relative to samples associated with colonization, those from pneumonia had significantly lower microbial diversity, decreased levels of several bacterial genera and prominent multifunctional cytokine responses. In contrast, tracheobronchitis was characterized by high microbial diversity and multifunctional cytokine responses that differed from those of pneumonia-colonization comparisons. The dissimilar microbiomes and cytokine responses underlying bacterial pneumonia and tracheobronchitis following lung transplantation suggest that the diseases result from different pathogenic processes. Microbiomes and cytokine responses had complementary features, suggesting that they are closely interconnected in the pathogenesis of both diseases.
Assuntos
Bronquite/diagnóstico , Líquido da Lavagem Broncoalveolar/microbiologia , Citocinas/metabolismo , Transplante de Pulmão/efeitos adversos , Microbiota , Pneumonia Bacteriana/diagnóstico , Traqueíte/diagnóstico , Adulto , Idoso , Teorema de Bayes , Bronquite/etiologia , Bronquite/metabolismo , Diagnóstico Diferencial , Feminino , Humanos , Masculino , Pessoa de Meia-Idade , Pneumonia Bacteriana/etiologia , Pneumonia Bacteriana/metabolismo , Estudos Retrospectivos , Traqueíte/etiologia , Traqueíte/metabolismo , TransplantadosRESUMO
Asthma is a common respiratory disease affecting â¼300 million people worldwide. Airway inflammation is thought to contribute to asthma pathogenesis, but the direct relationship between inflammation and airway hyperresponsiveness (AHR) remains unclear. This study investigates the role of inflammation in a steroid-insensitive, severe allergic airway disease model and in severe asthmatics stratified by inflammatory profile. First, we used the T-helper (T(H))-17 cells adoptive transfer mouse model of asthma to induce pulmonary inflammation, which was lessened by tumor necrosis factor (TNF)-α neutralization or neutrophil depletion. Although decreased airspace inflammation following TNFα neutralization and neutrophil depletion rescued lung compliance, neither intervention improved AHR to methacholine, and tissue inflammation remained elevated when compared with control. Further, sputum samples were collected and analyzed from 41 severe asthmatics. In severe asthmatics with elevated levels of sputum neutrophils, but low levels of eosinophils, increased inflammatory markers did not correlate with worsened lung function. This subset of asthmatics also had significantly higher levels of T(H)17-related cytokines in their sputum compared with severe asthmatics with other inflammatory phenotypes. Overall, this work suggests that lung compliance may be linked with cellular inflammation in the airspace, whereas T-cell-driven AHR may be associated with tissue inflammation and other pulmonary factors.
Assuntos
Asma/complicações , Inflamação/complicações , Pulmão/fisiologia , Adolescente , Adulto , Idade de Início , Idoso , Idoso de 80 Anos ou mais , Animais , Asma/imunologia , Asma/fisiopatologia , Hiper-Reatividade Brônquica/imunologia , Broncoconstritores/farmacologia , Criança , Citocinas/imunologia , Feminino , Humanos , Pulmão/efeitos dos fármacos , Pulmão/patologia , Masculino , Cloreto de Metacolina/farmacologia , Camundongos , Camundongos Transgênicos , Pessoa de Meia-Idade , Índice de Gravidade de Doença , Escarro/imunologiaRESUMO
PURPOSE: This study was conducted to investigate the role of testosterone (T) in regulating the relaxation of isolated rat corpus cavernosum strips in vitro. MATERIALS AND METHODS: Male rats were divided into treatment groups of intact, castrate, and castrate with T replacement. Norepinephrine (NE) was added to contract each of the tissue strips. Next, sodium nitroprusside (SNP), experiment I, or 8-Br-cGMP, experiment II, was added to relax the cavernosum tissue. Percent relaxations were recorded for each treatment group at each dose level. RESULTS: SNP was added to the NE-contracted tissues in doses of 10(-4) and 10(-3) M. In this experiment, castration significantly reduced tissue responsiveness to SNP and T replacement restored the response to intact levels. In the second experiment 8-Br-cGMP was added to the NE-contracted tissues in doses of 10(-5) and 10(-4) M. 8-Br-cGMP 10(-4) M was significantly less effective in relaxing tissue from castrate animals as compared with intact controls. Again, T treatment restored the response to intact levels. CONCLUSIONS: Our results show a clear role of T in regulating the ability of corpus cavernosum tissue to relax when treated with SNP or 8-Br-cGMP in vitro. In addition, the data suggests that T regulates sites distal to the formation of cGMP in the relaxation pathway.