Assuntos
Adjuvantes Imunológicos/uso terapêutico , Antioxidantes/uso terapêutico , Ácido Aspártico/uso terapêutico , Pneumoconiose/tratamento farmacológico , Compostos de Zinco/uso terapêutico , Adjuvantes Imunológicos/administração & dosagem , Antioxidantes/administração & dosagem , Asbestose/tratamento farmacológico , Ácido Aspártico/administração & dosagem , Humanos , Pneumoconiose/complicações , Silicose/tratamento farmacológico , Fatores de Tempo , Compostos de Zinco/administração & dosagemRESUMO
Platelet concentrates amounting to 142 X 10(9) +/- 11 X 10(9) cells are prepared by cytopheresis from one liter of blood. Ten of the concentrates are labelled with 51Cr and reinfused autologously. The other ten concentrates are frozen with a controlled rate freezer in a medium containing 10% DMSO and 5% glucose for 7 days at -196 degrees C. The fresh platelets have a 51Cr 24 hrs. recovery of 68 +/- 7% and a survival rate (T 1/2) of 10 +/- 1 days. The frozen preserved platelets have a 51Cr 24 hrs. recovery of 54 +/- 6.1%, a T 1/2 of 8.1 days, and significantly increased liver sequestration. There is a correlation between the reversal reaction in vitro after hypotonic shock and the 51Cr 24 hrs. in vivo recovery of fresh platelets and the 51Cr 1st hr. recovery of frozen platelets.
Assuntos
Plaquetas/fisiologia , Preservação de Sangue/métodos , Plaquetas/citologia , Transfusão de Sangue Autóloga , Sobrevivência Celular , Radioisótopos de Cromo , Congelamento , Humanos , Técnicas In Vitro , Fígado/citologia , Miocárdio/citologia , Transfusão de Plaquetas , Choque/sangue , Baço/citologiaRESUMO
Simultaneous vaccination was carried out of day-old chicks against Marek's disease (strain C3--1) and Newcastle disease (strains B1 and La Sota) which did not prove to be effective in conferring a lasting immunity to Newcastle disease though it was tolerated without any disturbances whatever. One could resort to it by way of an exception only, with an obligatory revaccination 15 days later, when the unfavourable epizootic status made it necessary. The immunity built up against Marek's disease did not seem to be affected by the simultaneous or succeeding vaccination against Newcastle disease with live lentogenic strains. Effective in conferring solid immunity against Newcastle disease proved the combined application of the two vaccines in the following order: treatment of day-old chicks with a vaccine against Marek's disease and vaccination of the same chicks at the age of 8 days with a vaccine against Newcastle disease (strain B1) at a half rate, employing the spray method, followed by revaccination 15 days later with the La Sota vaccine at a full rate, employing the aerosol method.