Application of CAD/CAM technologies and materials for prosthetic restoration of severely damaged teeth-clinical cases.

In cases of severely damaged teeth with limited coronal tooth structure and remaining hard dental tissues subgingivally, a custom-made post-and-core restoration is required. Teeth with non-circular canal space also require this type of restoration because the build-up with pre-fabricated posts could lead to thick cement layer. The development of CAD/CAM technologies widens the range of the materials that can be used for prosthetic restorations. Along with base dental alloys, newly developed materials may be applied. The aim of the article is to present four clinical cases of severely damaged teeth which utilize different materials and different production techniques for custom post-and-core fabrication. In the first clinical case, a metal post-and-core restoration was fabricated by direct metal laser sintering. In the second clinical case, digital technologies were used to produce a 3D-printed resin prototype for further investing and casting from base metal dental alloy. In the third clinical case, fibre-reinforced composite was used for fabrication of the custom post-and-core by milling. In the fourth clinical case, the restoration is produced by milling of lithium disilicate ceramics IPS emax CAD (Ivoclar Vivadent, Lichtenstein). The bond between the fibre-reinforced composite post-and-core and the hard dental tissues offered possibility to compensate-to some extent-the shape of the preparation which was not optimal. CAD/CAM technologies applied in these clinical cases provided combination of high accuracy of fitting with good stability and individual shape of the restorations. © 2023 Australian Dental Association.

A New Approach To the Diagnosis of Point Mutations in Native DNA Using Graphene Oxide.

Development of new methods for the diagnosis of point mutations is a pressing issue. We have developed a new approach to the design of graphene oxide-based test systems for the diagnosis of point mutations in native DNA. This new approach is based on the use of graphene oxide for the adsorption and quenching of fluorescently labeled primers in a post-amplification PCR mixture followed by detection of fluorescently labeled PCR products. It is possible to detect fluorescently labelled amplicons in the presence of an excess of primers in a PCR product solution due to the different affinities of single-stranded and double-stranded DNA molecules to graphene oxide, as well as the ability of graphene oxide to act as a quencher of the fluorophores adsorbed on its surface. The new approach was tested by designing a graphene oxide-based test system for the DNA diagnosis of the point mutation associated with the development of the 3M syndrome in Yakuts. The developed approach enables one to design graphene oxide-based test systems suitable for the diagnosis of any point mutations in native DNA.

The influence of climate on peatland extent in Western Siberia since the Last Glacial Maximum.

Boreal and subarctic peatlands are an important dynamical component of the earth system. They are sensitive to climate change, and could either continue to serve as a carbon sink or become a carbon source. Climatic thresholds for switching peatlands from sink to source are not well defined, and therefore, incorporating peatlands into Earth system models is a challenging task. Here we introduce a climatic index, warm precipitation excess, to delineate the potential geographic distribution of boreal peatlands for a given climate and landscape morphology. This allows us to explain the present-day distribution of peatlands in Western Siberia, their absence during the Last Glacial Maximum, their expansion during the mid-Holocene, and to form a working hypothesis about the trend to peatland degradation in the southern taiga belt of Western Siberia under an RCP 8.5 scenario for the projected climate in year 2100.