RESUMO
Giardia lamblia, a protozoan parasite, infects a wide variety of vertebrates, including humans. Studies indicate that this anaerobic protist possesses a limited ability to synthesize lipid molecules de novo and depends on supplies from its environment for growth and differentiation. It has been suggested that most lipids and fatty acids are taken up by endocytic and non-endocytic pathways and are used by Giardia for energy production and membrane/organelle biosynthesis. The purpose of this article is to provide an update on recent progress in the field of lipid research of this parasite and the validation of lipid metabolic pathways through recent genomic information. Based on current cellular, biochemical and genomic data, a comprehensive pathway has been proposed to facilitate our understanding of lipid and fatty acid metabolism/syntheses in this waterborne pathogen. We envision that the current review will be helpful in identifying targets from the pathways that could be used to design novel therapies to control giardiasis and related diseases.
Assuntos
Bases de Dados de Ácidos Nucleicos , Ácidos Graxos/metabolismo , Giardia lamblia/metabolismo , Metabolismo dos Lipídeos/genética , Proteínas de Protozoários/genética , Animais , Genoma/genética , Giardia lamblia/genética , Giardíase/parasitologia , Humanos , Proteínas de Protozoários/metabolismoRESUMO
The Giardia genome project database provides an online resource for Giardia lamblia (WB strain, clone C6) genome sequence information. The database includes edited single-pass reads, the results of BLASTX searches, and details of progress towards sequencing the entire 12 million-bp Giardia genome. Pre-sorted BLASTX results can be retrieved based on keyword searches and BLAST searches of the high throughput Giardia data can be initiated from the web site or through NCBI. Descriptions of the genomic DNA libraries, project protocols and summary statistics are also available. Although the Giardia genome project is ongoing, new sequences are made available on a bi-monthly basis to ensure that researchers have access to information that may assist them in the search for genes and their biological function. The current URL of the Giardia genome project database is www.mbl.edu/Giardia.
Assuntos
Bases de Dados Factuais , Genoma de Protozoário , Giardia/genética , AnimaisRESUMO
Protein-disulfide isomerase is essential for formation and reshuffling of disulfide bonds during nascent protein folding in the endoplasmic reticulum. The two thioredoxin-like active sites catalyze a variety of thiol-disulfide exchange reactions. We have characterized three novel protein-disulfide isomerases from the primitive eukaryote Giardia lamblia. Unlike other protein-disulfide isomerases, the giardial enzymes have only one active site. The active-site sequence motif in the giardial proteins (CGHC) is characteristic of eukaryotic protein-disulfide isomerases, and not other members of the thioredoxin superfamily that have one active site, such as thioredoxin and Dsb proteins from Gram-negative bacteria. The three giardial proteins have very different amino acid sequences and molecular masses (26, 50, and 13 kDa). All three enzymes were capable of rearranging disulfide bonds, and giardial protein-disulfide isomerase-2 also displayed oxidant and reductant activities. Surprisingly, the three giardial proteins also had Ca(2+)-dependent transglutaminase activity. This is the first report of protein-disulfide isomerases with a single active site that have diverse roles in protein cross-linking. This study may provide clues to the evolution of key functions of the endoplasmic reticulum in eukaryotic cells, protein disulfide formation, and isomerization.
Assuntos
Giardia lamblia/enzimologia , Isomerases de Dissulfetos de Proteínas/genética , Sequência de Aminoácidos , Animais , Sequência de Bases , DNA de Protozoário , Retículo Endoplasmático/enzimologia , Retículo Endoplasmático/ultraestrutura , Microscopia Eletrônica , Dados de Sequência Molecular , Isomerases de Dissulfetos de Proteínas/metabolismo , Homologia de Sequência de Aminoácidos , Transglutaminases/metabolismoRESUMO
The parasitic protozoan Giardia lamblia represents one of the earliest diverging lineages in the evolutionary history of eukaryotic organisms as well as an important human pathogen. A representative sampling of gene sequences from this early diverging protozoan could provide insights into genotypic and phenotypic innovations associated with the origin of eukaryotes. Currently, known giardial gene sequences are heavily biased toward a few gene families, including variant surface proteins (VSPs), structural proteins, and ribosomal RNA genes. One-pass sequences of Giardia genomic DNA were obtained using vector flanking priming sequences on the ends of cosmids in two independent libraries. Comparisons of 2304 of these sequences against the GenBank database identified 205 potential giardial genes with BLAST scores P(n) < 10(9). These coding regions encompass a wide range of metabolic, repair, and signaling enzymes, and include some genes not predicted by our current understanding of Giardia biochemistry. The efficiency of identification of putative genes is consistent with earlier findings that coding regions in the Giardia genome are densely packed and do not appear to contain introns. Our current results suggest that direct genome sequencing is an efficient method for identifying giardial genes for evolutionary and biochemical studies.
Assuntos
Genoma de Protozoário , Giardia lamblia/genética , Análise de Sequência de DNA , Animais , Cosmídeos/genética , Evolução Molecular , Biblioteca Gênica , Genes de Protozoários , Dados de Sequência Molecular , Monoéster Fosfórico Hidrolases/genética , Fosfotransferases/genética , Processamento de Proteína Pós-Traducional , Proteínas de Protozoários/genética , Proteínas de Protozoários/metabolismoRESUMO
Giardia lamblia trophozoites colonize the human small intestine, where they are exposed to high concentrations of conjugated bile acids. Previous work has shown that bile acids enhance trophozoite survival, multiplication, and differentiation into the cyst stage. Therefore, experiments were performed to test whether carrier-mediated uptake of conjugated bile acids is present in this primitive parasite. Uptake of both cholyltaurine (C-tau) and cholylglycine (C-gly) was increased manyfold after culturing trophozoites in medium lacking bile acids. Absence of uptake at 4 degrees C and inhibition by other conjugated bile acids provided additional evidence for carrier-mediated uptake. Uptake of C-tau was greater than that of C-gly under all experimental conditions and appeared to be mediated by a different carrier. The major evidence for different carriers is that C-tau uptake was Na(+)-dependent, while C-gly uptake was not. In addition, C-tau uptake was more strongly inhibited by DTNB and several organic anions than C-gly uptake. Radiolabeled C-tau and C-gly were each released rapidly from trophozoites at 37 degrees C but not at 4 degrees C, suggesting that release of conjugated bile acids was also carrier-mediated. These findings are consistent with the notion that multiple transporters for conjugated bile acids are present in a lower eukaryote. We speculate that intracellular bile acids may facilitate lipid trafficking and membrane biosynthesis.
Assuntos
Ácidos e Sais Biliares/metabolismo , Giardia lamblia/metabolismo , Animais , Transporte Biológico , Bovinos , Ácido Ditionitrobenzoico/farmacologia , Ditiotreitol/farmacologia , Giardia lamblia/efeitos dos fármacos , Ácido Glicocólico/metabolismo , Humanos , Reagentes de Sulfidrila/farmacologia , Ácido Taurocólico/metabolismoRESUMO
Lactoferrin and its derived N-terminal peptide may be important host defenses against Giardia lamblia. We showed earlier that lactoferrin and the derived peptides have potent giardicidal activity in vitro. Using indirect immunofluorescence, we now demonstrate binding of lactoferrin and the peptides to the live trophozoite surface. Iron strongly inhibited binding of lactoferrin, and decreased binding of the peptides, while certain divalent metal ions decreased binding of all forms by about half. Lactoferrin and the peptides caused striking and complex morphologic changes in the trophozoite plasmalemma, endomembranes and cytoskeleton, and increased the electron density of the lysosome-like peripheral vacuoles.
Assuntos
Giardia lamblia/metabolismo , Lactoferrina/metabolismo , Animais , Giardia lamblia/ultraestrutura , Fragmentos de Peptídeos/metabolismoRESUMO
Although encystation and excystation are crucial to transmission of Giardia lamblia, little is known about the regulation of these very distinct differentiation processes. Fingerprinting of giardial mRNA populations throughout the time course of differentiation demonstrated complex patterns in mRNA differential display. Certain transcripts appeared or increased, while others decreased or disappeared at specific times, in response to physiologic stimuli that mimic key stages in parasite descent through the host gastrointestinal tract. This approach has allowed the direct identification of critical stages in differentiation, as well as isolation of genes which may be crucial to the development of G. lamblia. One stage-specific single copy gene (ENC6) whose transcript is greatly upregulated during encystation was analyzed further. Partial sequence analysis revealed no correspondence with known genes. 3'-rapid amplification of cDNA ends (3'-RACE) analysis of ENC6 transcripts at various times of encystation revealed two polyadenylation sites. The more proximal site, 10 nucleotides past the single classic AGTAAA sequence, was utilized only during encystation and its transcript increased approximately 16-fold during the first 24 h of encystation. In contrast, a slightly divergent polyadenylation site 288 nucleotides downstream from the open reading frame (ORF) was used during both vegetative growth and encystation, although its transcript was present at low levels. These studies are the first evidence of differential mRNA processing in G. lamblia and suggest a potential role of the 3'-untranslated region (3'-UTR) in modulating gene expression during differentiation of this primitive eukaryote.
Assuntos
Giardia lamblia/genética , Giardia lamblia/metabolismo , RNA Mensageiro/genética , RNA Mensageiro/metabolismo , RNA de Protozoário/genética , RNA de Protozoário/metabolismo , Sequência de Aminoácidos , Animais , Sequência de Bases , Primers do DNA/genética , DNA de Protozoário/genética , Regulação da Expressão Gênica no Desenvolvimento , Genes de Protozoários , Giardia lamblia/crescimento & desenvolvimento , Dados de Sequência Molecular , Conformação de Ácido Nucleico , Processamento Pós-Transcricional do RNA , RNA Mensageiro/química , RNA de Protozoário/químicaAssuntos
Proteínas de Ligação ao Cálcio/genética , Proteínas Cromossômicas não Histona , Genes de Protozoários , Giardia lamblia/genética , Proteínas de Protozoários/genética , Sequência de Aminoácidos , Animais , Evolução Biológica , Proteínas de Ligação ao Cálcio/isolamento & purificação , Células Eucarióticas , Flagelos/ultraestrutura , Imunofluorescência , Giardia lamblia/ultraestrutura , Dados de Sequência Molecular , Proteínas de Protozoários/isolamento & purificação , Homologia de Sequência de AminoácidosRESUMO
Human and bovine lactoferrins and their derived N-terminal peptides were giardicidal in vitro. Fe3+, but not Fe2+, protected trophozoites from both native lactoferrin and peptides, although the latter lack iron-binding sites. Other divalent metal ions protected only against native lactoferrin. Log-phase cells were more resistant to killing than stationary-phase cells. These studies suggest that lactoferrin, especially in the form of the N-terminal peptides, may be an important nonimmune component of host mucosal defenses against Giardia lamblia.
Assuntos
Antiprotozoários/farmacologia , Giardia lamblia/efeitos dos fármacos , Giardíase/imunologia , Lactoferrina/farmacologia , Animais , Cátions Bivalentes , Bovinos , Giardia lamblia/crescimento & desenvolvimento , Humanos , Concentração de Íons de Hidrogênio , Metais/farmacologia , Fragmentos de Peptídeos/farmacologiaRESUMO
Although Giardia lamblia trophozoites were first described by Von Leeuwenhoek in his own diarrheic stool, relatively little is known of the basic biology of this common parasite or the pathophysiology of giardiasis. In particular, there is little specific information about trophozoite properties that cause diarrhea, as neither toxins nor conventional virulence factors have been identified. Therefore, parasite adaptations that promote cyst survival in the external environment and infection and trophozoite persistence in the small intestine, may be viewed as key virulence properties. This review focuses on unusual surface structures of the trophozoite and cyst forms that enable Giardia to be such a successful parasite.
Assuntos
Adaptação Fisiológica , Antígenos de Protozoários/análise , Antígenos de Superfície/análise , Giardia lamblia/fisiologia , Animais , Giardia lamblia/química , Giardia lamblia/ultraestrutura , Giardíase/fisiopatologia , Humanos , Proteínas de Protozoários/análiseRESUMO
Antimicrobial polypeptides such as the defensins kill a wide range of organisms, including bacteria, fungi, viruses, and tumor cells. Because of the recent finding that intestinal defensins, also known as cryptdins, are synthesized by the Paneth cells of the small intestinal crypts and released into the lumen, we asked whether defensins and other small cationic antimicrobial peptides could kill the trophozoites of Giardia lamblia, which colonize the small intestine. Four mouse cryptdins, two neutrophil defensins (HNP-1 [human] and NP-2 [rabbit]), and the unique tryptophan-rich bovine neutrophil polypeptide indolicidin each had some antigiardial activity against trophozoites in vitro. Cryptdins 2 and 3, indolicidin, and NP-2 each reduced viability by more than 3 log units in 2 h, and killing by all peptides was dose and time dependent. Exposure of trophozoites to peptides frequently resulted in cell aggregation and dramatic changes in morphology. The mechanism of binding and lysis appeared to involve charge interactions, since 150 mM NaCl as well as millimolar levels of Ca2+ and Mg2+ inhibited killing by most of the peptides. Our studies show that G. lamblia is sensitive to defensins and indolicidin and suggest that these small polypeptides could play a role in nonimmune host defenses.
Assuntos
Antibacterianos/farmacologia , Peptídeos Catiônicos Antimicrobianos , Proteínas Sanguíneas/farmacologia , Giardia lamblia/efeitos dos fármacos , Peptídeos/farmacologia , Precursores de Proteínas/farmacologia , Proteínas/farmacologia , alfa-Defensinas , beta-Defensinas , Sequência de Aminoácidos , Animais , Cátions/farmacologia , Defensinas , Relação Dose-Resposta a Droga , Giardia lamblia/citologia , Giardia lamblia/crescimento & desenvolvimento , Giardíase/imunologia , Imunidade Inata , Dados de Sequência MolecularRESUMO
Giardia lamblia is a primitive protozoan and a major cause of waterborne enteric disease throughout tropical and temperate zones. The ability to grow the infective trophozoites in culture as well as the discovery of the method of in vitro encystation made it possible to study the biology of this primitive protozoan and to characterize the surface antigens. Giardia trophozoites are exposed to high concentrations of fatty acids in the human small intestine. This raises the possibility that intestinal fatty acids may become incorporated into Giardia. Therefore, we determined the pattern of fatty acylation of Giardia surface molecules. By metabolic labeling with radiolabeled fatty acids we identified a single glycosylphosphatidylinositol (GPI)-anchored surface protein in Giardia. GP49 differs from the cysteine-rich variable surface antigens described previously. The presence of a GPI anchor in GP49 was supported by the metabolic incorporation of [14C]-ethanolamine, [3H]-myoinositol and fatty acids into the protein. This was confirmed by chemical and enzymatic cleavage experiments. Most interestingly, GP49 was found to be present in different isolates of Giardia and thus can be considered as an invariant surface antigen. Although the biological function of GP49 is not known, recently we have found that intact and soluble GP49 altered the electrolyte fluxes which regulate fluid secretion in the cultured human intestinal epithelial cell line, T84. These studies indicate that the GPI-anchored invariant antigen of Giardia may play an important role in the pathophysiology of giardiasis.
Assuntos
Antígenos de Protozoários/análise , Antígenos de Superfície/análise , Giardia lamblia/imunologia , Glicosilfosfatidilinositóis/química , Animais , Antígenos de Protozoários/química , Antígenos de Superfície/química , Células Cultivadas , Ácidos Graxos/metabolismo , Giardia lamblia/crescimento & desenvolvimento , Giardia lamblia/metabolismo , Humanos , Técnicas In Vitro , Fosfolipases/metabolismo , Glicoproteínas Variantes de Superfície de Trypanosoma/imunologiaRESUMO
Giardia lamblia develops and thrives within the harsh and variable environment of the human small intestine. To survive this environment, Giardia has evolved a unique family of antigenically variable, extremely cysteine-rich surface proteins. We have characterized the expression of one of these antigens, TSA 417, at the gene and protein levels. TSA 417 mRNA and protein were expressed at constant levels during both logarithmic growth and encystation. On the trophozoite surface, TSA 417 exists in two forms, an 85-kDa protein and a derived 66-kDa form. Both species have identical amino-terminal sequences that match the translated sequence of TSA 417 after removal of a predicted 17 amino acid signal peptide. Since TSA 417 is highly cysteine rich (ca. 12 mole%), we asked whether it was complexed with itself or with other molecules. Although we found no interchain disulfide bonds, there was substantial intrachain bonding that helped retain the gross structure of isolated TSA 417 after partial trypsin digestion. Because the hydropathy profile of TSA 417 suggested that most of the cysteine and all 29 of its conserved CXXC motifs are on the cell surface, we investigated whether TSA 417 might contain some of the free thiols previously shown to be on the trophozoite surface (Gillin et al., Molecular and Biochemical Parasitology, 13, 1-12, 1984). However, no free thiols were detected, either exposed on native TSA 417 or in the unfolded protein. The absence of free thiols and resistance to proteolytic digestion suggest that most of the cysteine residues are in intrachain bridges, probably either disulfide bonds or cysteine-metal complexes. This internal crosslinking may help explain the resistance of Giardia to proteases and other degradative enzymes in the intestinal fluid.
Assuntos
Antígenos de Protozoários/metabolismo , Antígenos de Superfície/metabolismo , Cisteína/química , Giardia lamblia/imunologia , Processamento de Proteína Pós-Traducional , Proteínas de Protozoários , Sequência de Aminoácidos , Animais , Antígenos de Protozoários/química , Antígenos de Protozoários/genética , Antígenos de Superfície/química , Antígenos de Superfície/genética , Northern Blotting , Southern Blotting , Cisteína/metabolismo , DNA de Protozoário/análise , Expressão Gênica , Giardia lamblia/genética , Dados de Sequência Molecular , Peso Molecular , RNA Mensageiro/biossíntese , RNA de Protozoário/biossíntese , Tripsina/metabolismoRESUMO
The abundant, highly variable surface proteins (VSPs) which cover the surface of Giardia lamblia trophozoites compose a group of extremely cysteine (C)-rich proteins in which more than half of the cysteines are in the motif CXXC. Because of the constancy of these features among the known VSPs and the prominence of cysteine and particularly CXXC in proteins that bind zinc and other metals, we asked whether G. lamblia VSPs bind zinc in vitro. VSPs are the major protein component of Triton X-114 detergent-phase extracts of G. lamblia trophozoites and can be readily identified by surface iodination of intact cells. The partitioning of 65Zn binding into the Triton X-114 detergent phase and the correspondence between surface iodination and zinc binding patterns of four G. lamblia strains or sublines with different VSPs support the idea that VSPs bind zinc. The requirement for renaturation of blots with a reducing agent indicates that Zn2+ is coordinated by cysteine residues, rather than by other amino acids. Binding did not appear to be specific to zinc since it was inhibited by competition with other divalent metal ions. The abundance of the VSPs and the prevalence of metal binding motifs among all known variants suggest that they may play an important role in trophozoite survival and colonization in the host.
Assuntos
Antígenos de Protozoários/metabolismo , Antígenos de Superfície/metabolismo , Cisteína/fisiologia , Giardia lamblia/metabolismo , Proteínas de Protozoários , Zinco/metabolismo , Animais , Proteínas de Transporte/análiseRESUMO
Sporozoan parasites of the phylum Apicomplexa all possess common apical structures. The current study used a monoclonal antibody (mAb-E12) to identify a conserved antigen in the apical region of merozoites of seven species of Plasmodium (including rodent, primate and human pathogens), tachyzoites of Toxoplasma gondii, bradyzoites of Sarcocystis bovis, and sporozoites and merozoites of Eimeria tenella and E. acervulina. The antigen was also present in sporozoites of haemosporinid parasites. Immunofluorescence studies showed that the antigen was restricted to the apical 3rd of these invasive stages. Using immunoelectron microscopy, labeling was demonstrated in the region of the polar ring, below the paired inner membranes of the parasite pellicle, and near the subpellicular microtubules radiating from the polar ring of merozoites and sporozoites of E. tenella. The majority of the antigen could be extracted with 1% Triton-X 100, but a portion remained associated with the cytoskeletal elements. The molecule has a relative rate of migration (Mr) of 47,000 in Plasmodium spp. and 43-46,000 in coccidian species. Since the epitope recognized by mAb-E12 is highly conserved, restricted to motile stages, and appears to be associated with microtubules, this antigen could be involved in cellular motility and cellular invasion.
Assuntos
Antígenos de Protozoários/análise , Apicomplexa/imunologia , Animais , Anticorpos Monoclonais , Antígenos de Protozoários/imunologia , Apicomplexa/ultraestrutura , Western Blotting , Eletroforese em Gel de Poliacrilamida , Imunofluorescência , Microscopia ImunoeletrônicaRESUMO
To study the interactions between the parasitic protozoan Giardia lamblia and its environment, we have cloned the gene that encodes the two major surface-labeled trophozoite protein species. Sequence analysis of this gene reveals a single open reading frame specifying a hydrophilic, cysteine-rich (11.8%) protein of 72.5-kDa molecular mass with an amino-terminal signal peptide and a postulated hydrophobic membrane-spanning anchor region near the carboxyl terminus. Most of the cysteine residues (58 of 84) are in the motif Cys-Xaa-Xaa-Cys, which is dispersed 29 times throughout the sequence. Antibodies against the recombinant protein react with the entire surface of live trophozoites, including flagella and adhesive disc. These antibodies inhibit trophozoite attachment, prevent growth, and immunoprecipitate the major approximately 66- and 85-kDa proteins from surface-labeled live trophozoites. The recombinant Escherichia coli also expresses polypeptides of approximately 66- and 85-kDa molecular mass, which are not fusion proteins. This suggests that the processing and/or conformational changes that lead to production of these two peptide species in E. coli reflect those that occur in Giardia. The abundance of cysteine residues suggests that the native proteins on the parasite surface may contain numerous disulfide bonds, which would promote resistance to intestinal fluid proteases and to the detergent activity of bile salts and would help to explain the survival of Giardia in the human small intestine.
Assuntos
Antígenos de Protozoários/genética , Genes , Giardia/genética , Proteínas de Membrana/genética , Sequência de Aminoácidos , Animais , Anticorpos Antiprotozoários , Complexo Antígeno-Anticorpo , Sequência de Bases , DNA/genética , Biblioteca Genômica , Giardia/imunologia , Dados de Sequência MolecularRESUMO
The intracellular distribution of circumsporozoite (CS) antigen was traced by immunoelectron microscopy in cultures of Plasmodium berghei exoerythrocytic (EE) schizonts with monoclonal antibody (Mab) 3D11 to the immunodominant repeat region of the P. berghei CS protein. CS antigen was localized on the parasitophorous vacuole (PV) membrane and pellicular complex of recently invaded sporozoites and on electron-dense masses of sloughed CS antigen in the host cell cytoplasm. CS antigen persisted throughout the complete EE cycle of P. berghei on the surface of EE schizonts and was incorporated into the plasma membrane of budding EE merozoites. Erythrocytic merozoites were not labeled by Mab 3D11, indicating that these 2 populations of merozoites differ in antigenic composition. Significant internal labeling occurred in 50 hr EE schizonts in association with the limiting membranes of peripheral vesicles and short, tube-like structures attached to their outer surfaces. These vesicles contained an electron-dense flocculent material also present in the PV space. Association of CS antigen with the limiting membranes of these vesicles suggests that they either develop as endocytotic invaginations of the schizont plasma membrane or transport newly synthesized CS antigen from the endoplasmic reticulum and Golgi of developing EE schizonts to the parasite surface.
Assuntos
Antígenos de Protozoários/fisiologia , Antígenos de Superfície/análise , Plasmodium berghei/imunologia , Proteínas de Protozoários , Animais , Antígenos de Protozoários/análise , Antígenos de Superfície/fisiologia , Diferenciação Celular , Células Cultivadas , Microscopia EletrônicaRESUMO
The fate and disposition of the circumsporozoite (CS) protein of Plasmodium falciparum was investigated during hepatoma cell invasion with several sera raised against defined CS peptides, including both repeat and nonrepeat regions spanning approximately 60% of the P. falciparum CS gene product. Distribution of the protein, as revealed by immunoelectron microscopy, was limited to the surface of the sporozoite both before and after invasion. In particular, no CS protein antigen was detected in association with either the parasitophorous vacuole membrane or the host cell surface.
Assuntos
Antígenos de Protozoários/análise , Antígenos de Superfície/análise , Fígado/parasitologia , Plasmodium falciparum/ultraestrutura , Proteínas de Protozoários , Animais , Carcinoma Hepatocelular , Humanos , Soros Imunes/imunologia , Imunoensaio , Imuno-Histoquímica , Neoplasias Hepáticas , Microscopia Eletrônica , Plasmodium falciparum/imunologia , Células Tumorais CultivadasAssuntos
Antígenos de Protozoários/imunologia , Antígenos de Superfície/metabolismo , Interações Hospedeiro-Parasita , Peptídeos/metabolismo , Plasmodium/metabolismo , Proteínas de Protozoários , Sequência de Aminoácidos , Animais , Anticorpos Monoclonais/imunologia , Antígenos de Superfície/imunologia , Epitopos/imunologia , Humanos , Camundongos , Camundongos Endogâmicos C57BL , Peptídeos/imunologia , Plasmodium/imunologia , Vacinas Sintéticas/imunologiaRESUMO
Exoerythrocytic parasites of Plasmodium vivax grown in human hepatoma cells in vitro were probed with monoclonal antibodies raised against other stages of P. vivax. Monoclonal antibodies specific for four independent antigens on blood-stage merozoites all reacted with exoerythrocytic schizonts and merozoites by immunostaining. The characteristic staining pattern of each monoclonal antibody was similar on both blood- and exoerythrocytic-stage parasites and appeared only in mature schizont segmenters. In contrast, a monoclonal antibody specific for the caveolar-vesicle complex of the infected host cell membrane and a second monoclonal antibody reacting with an unknown internal antigen did not appear to react with exoerythrocytic parasites. We confirm prior reports that monoclonal antibodies against the sporozoite immunodominant repeat antigen react with all exoerythrocytic-stage parasites, but note that as the exoerythrocytic parasite matures the immunostaining is concentrated in plaques reminiscent of germinal centers and apparently distinct from mature merozoites. These results indicate that mature merozoites from either exoerythrocytic or blood-stage parasites are antigenically very similar, but that stage-specific antigens may be found in specialized structures present only in a specific host cell type.