The Effectiveness of Nigella sativa and Ginger as Appetite Suppressants: An Experimental Study on Healthy Wistar Rats.

Background: Obesity is a global pandemic that is associated with high morbidity and mortality. Natural herbs are commonly used for weight reduction and appetite suppression. Therefore, we aim to investigate the role and mechanism of Nigella sativa (NS) and ginger on weight reduction and appetite regulation. Methods: This experimental study was performed at Imam Abdulrahman Bin Faisal University. Twenty-five female rats were distributed into 5 groups: NS (oral 1000mg/kg), Ginger (500 mg/kg), NS-ginger (both interventions), a positive control (intraperitoneal 50 µg/kg Liraglutide), and a negative control. Each intervention was given for 9 weeks. Food intake and body weight were assessed weekly. Serum lipid profile and peptides involved in appetite control (cholecystokinin (CCK), glucagon-like peptide 1(GLP-1), gastric inhibitory polypeptide (GIP), ghrelin, peptide YY, and orexin) were assayed at the end of the experiment. Results: None of the interventions showed a statistically significant difference regarding food consumption or weight gain (p > 0.05). However, the three interventions significantly reduced total cholesterol (TC), NS and NS-ginger significantly increased HDL, NS increased ghrelin and ginger increased orexin. Conclusion: The present dose and duration of NS, ginger, or in combination did not demonstrate a significant change in body weight or food consumption in comparison to the negative or positive controls. However, NS or ginger has improved the lipid profile by reducing TC and increasing HDL. In addition, NS or ginger can influence some of the peptides involved in appetite regulation such as the increase in ghrelin induced by NS and the reduction of orexin induced by ginger. We believe that these latter effects are novel and might indicate a promising effect of these natural products on appetite regulation.

Assessing the Impact of Alopecia on Quality of Life, Depression, and Self-Esteem in Saudi Arabia.

Objectives The objective of this study is to assess the effects of alopecia on quality of life, depression, and self-esteem in the Eastern Province of Saudi Arabia. Methods We made a questionnaire that included sociodemographic data, type of alopecia, medical aid sought, and whether they benefited from it or not. The study uses the Dermatology Life Quality Index, Patient Health Questionnaire-9, and a single-item self-esteem scale to assess the quality of life, depression, and self-esteem, respectively. Results The questionnaire was completed by 403 individuals in total, and after applying the exclusion criteria (only Saudis, from the Eastern Province), 231 participants were included in this study. Of the participants, 49.4% had hereditary baldness and only 9.5% benefited from treatment. Of the patients, 52.4% had higher levels of depression, and 18.2% had an effect on their life. Patients with a moderate or greater effect on their lives had a significant relationship with hereditary baldness. Younger age, being female, being married, having lower income, and having hereditary baldness were significantly associated with higher depression levels (p = <0.05). The study found that as age increased, depression levels decreased and self-esteem scores increased. Depression was linked to lower quality of life, while self-esteem was linked to both lower quality of life and higher depression levels. These factors are interrelated, with age influencing their relationship. Conclusions The results of the study highlight the significant occurrence of depression and decreased quality of life among patients who have alopecia, particularly those with hereditary baldness. It is crucial to provide psychological assistance and counseling to enhance their mental health and overall wellness.

Potential Antigenic Candidates for the Development of Peptide-Based Vaccines to Induce Immunization against Helicobacter pylori Infection in BALB/c Mice.

Helicobacter pylori (H. pylori) has been identified as a group-1 definite carcinogen. As of yet, there is no available vaccine for this microorganism. Our study aimed to identify antigenic peptides in H. pylori using an in silico proteomic approach, and to evaluate their effectiveness as potential vaccine candidates. Four different peptide sequences were prioritized using the reverse vaccinology, namely, CagA1, CagA2, VacA, and SabA. Peptides emulsified with Freunde's adjuvant were used to immunize BALB/C mice. Subcutaneously immunized mice were challenged by oral administration of H. pylori. IgG, IgA, IL4, and IL17 were detected in mice sera. Histopathology of the dissected stomach of vaccinated and control mice were assessed using H&E stain. IgG was significantly higher in mice vaccinated with SabA. IL-4 was significantly increased in CagA1, CagA2, VacA, and SabA vaccinated mice compared to the adjuvant group. Additionally, histopathological examination of gastric tissue showed a protective effect in the vaccinated groups compared to adjuvant and PBS groups. Our findings indicate a promising effect of the tested epitopes, particularly the SabA antigen, to induce an immune response against H. pylori.