Análise comparativa da técnica de baciloscopia no diagnósticoda tuberculose pulmonar frente ao GeneXpert em amostras depacientes da cidade de Recife, Pernambuco / Comparative analysis of the bacilloscopy technique in the diagnosis of pulmonary tuberculosis against GeneXpert in samples of patients in the city of Recife, Pernambuco

Objetivo: Considerando que atualmente ainda existem algumas limitações quanto à escolha do teste ideal para diagnóstico da TB, ligadas principalmente aos custos e realização adequada das técnicas, o presente estudo teve por objetivo verificar a eficácia do teste de baciloscopia no diagnóstico da tuberculose pulmonar, a partir da comparação dos resultados obtidos através dos testes de baciloscopia e GeneXpert de pacientes da cidade de Recife-PE. Métodos: Foram analisadas neste estudo amostras de escarro com os resultados já positivos para TB através da cultura microbiológica. As espécimes foram submetidas ainda aos testes de baciloscopia e GeneXpert para uma análise comparativa dos resultados. Resultados: Foi analisado um total de 100 amostras, com positividade para todos os testes realizados no estudo (cultura microbiológica, GeneXpert e baciloscopia). Conclusão: O diagnóstico através do GeneXpert representa um ganho expressivo quando levado em conta sua sensibilidade, rapidez e confiabilidade dos resultados, em contrapartida, os custos para realização deste teste ainda são elevados. Este fato reforça a importância das metodologias convencionais de baciloscopia e cultura, quando utilizadas dentro dos parâmetros de controle de qualidade adequados.

Objective: Considering that currently there are still some limitations regarding the choice of the ideal test for diagnosis of TB, related mainly to costs and adequate performance of the techniques, the present study aimed to verify the efficacy of the bacilloscopy test in the diagnosis of pulmonary tuberculosis, based on the comparison of the results obtained through the bacilloscopy and GeneXpert tests of patients from the city of Recife-PE. Methods: Sputum samples with already positive results for TB were analyzed in this study through the microbiological culture. The specimens were also submitted to bacilloscopy and GeneXpert tests for a comparative analysis of the results. Results: A total of 100 samples were analyzed, with positivity for all tests performed in the study (microbiological culture, GeneXpert and smear microscopy). Conclusion: The diagnosis through GeneXpert represents an expressive gain when taking into account its sensitivity, speed and reliability of results, in contrast, the costs to perform this test are still high. This fact reinforces the importance of conventional bacilloscopy and culture methodologies when used within the appropriate quality control parameters

Multidrug-resistant Acinetobacter baumannii clones persist on hospital inanimate surfaces

ABSTRACT Acinetobacter baumannii is one of the most frequent Gram-negative opportunistic pathogens associated with hospital-acquired infection worldwide. We briefly describe A. baumannii isolates that were recovered from surrounding ICU bed surfaces, exhibiting multidrug resistance phenotype and belonging to some widely spread clonal complexes of clinical A. baumannii isolates.

Multidrug-resistant Acinetobacter baumannii clones persist on hospital inanimate surfaces.

Acinetobacter baumannii is one of the most frequent Gram-negative opportunistic pathogens associated with hospital-acquired infection worldwide. We briefly describe A. baumannii isolates that were recovered from surrounding ICU bed surfaces, exhibiting multidrug resistance phenotype and belonging to some widely spread clonal complexes of clinical A. baumannii isolates.