Gall induction may benefit host plant: a case of a gall wasp and eucalyptus tree.

Gall-inducing insects display intimate interactions with their host plants, usually described as parasitic relationships; the galls seem to favor the galler alone. We report on a case in which the presence of the galls induced by Leptocybe invasa Fisher & LaSalle (Hymenoptera; Eulophidae) benefit its host plant, the river red gum Eucalyptus camaldulensis Dehnh. Field observations showed that E. camaldulensis plants infected by this gall wasp were less susceptible to cold injury than neighboring conspecific plants without galls. In the laboratory, frost resistance was compared between galled and non-galled plants which were both divided into two subgroups: cold-acclimated plants and plants that were non-acclimated. Galled plants displayed higher frost resistance than the non-galled ones, and the differences were higher in non-acclimated plants compared with acclimated ones. Physiological changes in host plant were determined by chemical analyses of chlorophylls, proteins, soluble sugars and anthocyanin contents. The results showed higher values of all physiological parameters in the galled plants, supporting the hypothesis that the presence of the gall wasp induces physiological changes on the plant foliage, which may in turn increase plant defense mechanisms against cold. Therefore, the toll of galling by the herbivore may pay off by the host plant acquiring increased frost resistance. This work provides evidence for physiological changes induced by a herbivore which might have a positive indirect effect on the host plant, promoting frost resistance such as cold acclimation.

Pancreatic autoantibodies, HLA DR and PTPN22 polymorphisms in first degree relatives of patients with type 1 diabetes and multiethnic background.

AIM: To evaluate the prevalence of pancreatic auto-antibodies (PAb) as well as its relationship with HLA DR B1 and PTPN22 polymorphisms in first degree relatives (FDR) of Brazilian patients with Type 1 diabetes (T1D) and multiethnic background. METHODS: FDR of patients with T1D were interviewed and blood was sampled for PAb measurement, HLA DRB1 and PTPN22 genotyping. Genotyping was also performed in index cases. RESULTS: In FDR (n=78), 16.7% presented at least one PAb. These individuals had a higher prevalence of HLA DRB1* 03 than others (p=0.03), without differences in PTPN22 genotyping. While the genetic profile was similar in FDR with PAb and their index cases, those without PAb had a lower frequency of HLA DR B1 * 03 than their correspondent patients (p=0.009). CONCLUSION: In this multiethnic population, a significant proportion of FDR of T1D patients had PAb, which was associated with HLA DR B1 * 03 but not with the PTPN22 polymorphism.

Acclimation to short-term low temperatures in two Eucalyptus globulus clones with contrasting drought resistance.

We tested the hypothesis that Eucalyptus globulus Labill. genotypes that are more resistant to dry environments might also exhibit higher cold tolerances than drought-sensitive plants. The effect of low temperatures was evaluated in acclimated and unacclimated ramets of a drought-resistant clone (CN5) and a drought-sensitive clone (ST51) of E. globulus. We studied the plants' response via leaf gas exchanges, leaf water and osmotic potentials, concentrations of soluble sugars, several antioxidant enzymes and leaf electrolyte leakage. Progressively lowering air temperatures (from 24/16 to 10/-2 degrees C, day/night) led to acclimation of both clones. Acclimated ramets exhibited higher photosynthetic rates, stomatal conductances and lower membrane relative injuries when compared to unacclimated ramets. Moreover, low temperatures led to significant increases of soluble sugars and antioxidant enzymes activity (glutathione reductase, ascorbate peroxidase and superoxide dismutases) of both clones in comparison to plants grown at control temperature (24/16 degrees C). On the other hand, none of the clones, either acclimated or not, exhibited signs of photoinhibition under low temperatures and moderate light. The main differences in the responses to low temperatures between the two clones resulted mainly from differences in carbon metabolism, including a higher accumulation of soluble sugars in the drought-resistant clone CN5 as well as a higher capacity for osmotic regulation, as compared to the drought-sensitive clone ST51. Although membrane injury data suggested that both clones had the same inherent freezing tolerance before and after cold acclimation, the results also support the hypothesis that the drought-resistant clone had a greater cold tolerance at intermediate levels of acclimation than the drought-sensitive clone. A higher capacity to acclimate in a short period can allow a clone to maintain an undamaged leaf surface area along sudden frost events, increasing growth capacity. Moreover, it can enhance survival chances in frost-prone sites expanding the plantation range with more adaptive clones.

Family history of type 2 diabetes is increased in patients with type 1 diabetes.

It has been suggested that type 1 (T1D) and type 2 diabetes (T2D) might share some susceptibility risk factors. A higher prevalence of T2D has been reported in families of Caucasian T1D children than in the general population, although data in adults and multiethnic groups is still lacking. Our goal was to compare the prevalence of T2D family history between adults with T1D from a multiethnic population and a non-diabetic control group. We performed a cross-sectional analysis of 145 adults with T1D and 141 healthy adults (control group) that included an interview and a review of the medical charts. Groups were matched for age, sex, ethnicity and body mass index (BMI). We found a higher prevalence of not only T1D but also T2D in first-degree relatives of patients than in controls (p<0.001 and p=0.042, respectively). These differences were not observed for second/third-degree relatives. When subjects were stratified according to their ethnicity, the higher frequency of T2D in FDR of patients than controls became more striking in non-white (p=0.002) and disappeared in white individuals (p=0.85). To conclude, the prevalence of T1D and T2D was higher in first-degree relatives of patients with T1D than of controls. The difference in T2D family history between patients and controls was specially striking among non-whites, which may represent a peculiarity of T1D in this group.

Acute thyroiditis due to septic emboli derived from infective endocarditis.

Acute infectious thyroiditis is a rare condition of the thyroid gland, most often arising in children with congenital conditions connecting the thyroid directly to the oropharynx, such as a piriform fistula or thyroglossal duct. We report a case of acute thyroiditis due to septic emboli derived from infective endocarditis.

[Pulmonary function in Portuguese firefighters]. / Função respiratória em bombeiros portugueses.

INTRODUCTION: Portugal has had a high rate of forest fires in recent years. Inhaled wood smoke can have short- and long-term effects on the lung function of people exposed to it. STUDY OBJECTIVES: To assess the lung function of active wildland (forest) firefighters. METHODS: Cross-sectional study. A self-questionnaire on personal and work habits was used and spirometry values were obtained using Piko-6 for a 209 people sample. RESULTS: We found a high rate of smoking (42.9%) and an 11.8% prevalence of obstruction. 41.7% of the obstructed individuals were non-smokers, did not state a knowledge of any respiratory disease, engaged in no other activity that could be related to lung function decrease and did not wear airway protection apparatus. 85.7% did not use any airway protection apparatus when firefighting due to lack of such equipment in their brigades. CONCLUSIONS: Data showed that there is a high pre- valence of smoking habits in this sample of Portuguese firefighters; there is an unsatisfactory usage of airway protection apparatus and the prevalence of airway obstruction is higher than the COPD prevalence in the Portuguese population. We recommend stopping smoking and use of equipment for respiratory protection.

Metabolic responses to water deficit in two Eucalyptus globulus clones with contrasting drought sensitivity.

We compared the metabolic responses of leaves and roots of two Eucalyptus globulus Labill. clones differing in drought sensitivity to a slowly imposed water deficit. Responses measured included changes in concentrations of soluble and insoluble sugars, proline, total protein and several antioxidant enzymes. In addition to the general decrease in growth caused by water deficit, we observed a decrease in osmotic potential when drought stress became severe. In both clones, the decrease was greater in roots than in leaves, consistent with the observed increases in concentrations of soluble sugars and proline in these organs. In roots of both clones, glutathione reductase activity increased significantly in response to water deficit, suggesting that this enzyme plays a protective role in roots during drought stress by catalyzing the catabolism of reactive oxygen species. Clone CN5 has stress avoidance mechanisms that account for its lower sensitivity to drought compared with Clone ST51.

Responses to water stress in two Eucalyptus globulus clones differing in drought tolerance.

We evaluated drought resistance mechanisms in a drought-tolerant clone (CN5) and a drought-sensitive clone (ST51) of Eucalyptus globulus Labill. based on the responses to drought of some physiological, biophysical and morphological characteristics of container-grown plants, with particular emphasis on root growth and hydraulic properties. Water loss in excess of that supplied to the containers led to a general decrease in growth and significant reductions in leaf area ratio, specific leaf area and leaf-to-root area ratio. Root hydraulic conductance and leaf-specific hydraulic conductance decreased as water stress became more severe. During the experiment, the drought-resistant CN5 clone maintained higher leaf water status (higher predawn and midday leaf water potentials), sustained a higher growth rate (new leaf area expansion and root growth) and displayed greater carbon allocation to the root system and lower leaf-to-root area ratio than the drought-sensitive ST51 clone. Clone CN5 possessed higher stomatal conductances at moderate stress as well as higher hydraulic conductances than Clone ST51. Differences in the response to drought in root biomass, coupled with changes in hydraulic properties, accounted for the clonal differences in drought tolerance, allowing Clone CN5 to balance transpiration and water absorption during drought treatment and thereby prolong the period of active carbon assimilation.

Cold acclimation in eucalypt hybrids.

We evaluated cold resistance and the capacity for cold acclimation of different Eucalyptus genotypes. Seedlings of half-sib families of E. globulus and hybrids E. gunnii x globulus, E. viminalis x globulus and E. cypellocarpa x globulus were exposed daily for 56 days to a 9-h photoperiod at 14.7 degrees C, followed by 15 h in a dark cold room maintained at 2.5 degrees C with the root system maintained at 8 degrees C to cold harden the seedlings. Unhardened seedlings were maintained at about 16 degrees C during the dark period. Cold acclimation occurred in all families with decreases in the temperature causing 50% mortality (LT(50)) of between 1.5 and 3 degrees C. Both hardened and unhardened plants of hybrid families were more cold tolerant than E. globulus. A significant correlation between LT(50) and leaf osmotic pressure was observed; the increase in osmotic pressure in hardened plants was predominantly a result of an increase in the concentration of soluble sugars. Exotherm peaks were similar in hardened and unhardened plants. These results indicate that cold hardening increased the ability of eucalypts to endure extracellular ice formation. The maintenance of photosynthetic capacity in cold-hardened plants may also play a role in their response to freezing.