A novel electrochemical biosensor based on palladium nanoparticles decorated on reduced graphene oxide-polyaminophenol matrix for the detection and discrimination of mitomycin C-DNA and acyclovir-DNA interaction.

Both the design of molecules that will interact specifically with DNA and the determination of the mechanism of action of this drug on DNA are important as they allow the control of gene expression. In particular, rapid and precise analysis of this type of interaction is a vital element for pharmaceutical studies. In the present study, a novel reduced graphene oxide/ palladium nanoparticles/ poly(2-amino-4-chlorophenol) (rGO/Pd@PACP) nanocomposite was synthesized by chemical process to modify pencil graphite electrode (PGE) surface. Here, the performance of the newly developed nanomaterial-based biosensor for drug-DNA interaction analysis has been demonstrated. For this purpose, it was determined whether this system, which was developed by selecting a drug molecule (Mitomycin C; MC) known to interact with DNA and a drug molecule (Acyclovir; ACY) that does not interact with DNA, performs a reliable/accurate analysis. Here, ACY was used as a negative control. Compared to bare PGE, the rGO/Pd@PACP nanomaterial modified sensor exhibited 17 times higher sensitivity performance in terms of guanine oxidation signal measured by differential pulse voltammetry (DPV). Moreover, the developed nanobiosensor system provided a highly specific determination between the anticancer drug MC and ACY by discrimination the interactions of these drugs with double-stranded DNA (dsDNA). ACY was also preferred in studies for the optimization of the new nanobiosensor developed. ACY was detected in a concentration as low as 0.0513 µM (51.3 nM) (LOD), and limit of quantification (LOQ) was 0.1711 µM with a linear range from 0.1 to 0.5 µM.

Tb(III)-DO3A and BODIPY dyad as multimode responsive hypochlorite probe.

A multimode responsive hypochlorite probe 1, based on Terbium (III)-1,4,7,10-tetraazacyclododecane-1,4,7-triacetic acid (Tb(III)-DO3A) and 4,4-difluoro-4-bora-3a,4a-diaza-s-indacene (BODIPY), is described. We have shown that probe 1 can detect ClO- by absorption, fluorescence, and phosphorescence simultaneously. The multimode response makes probe 1 a versatile ClO- probe for practical applications. We have found that probe 1 can be used in naked-eye colorimetric and fluorogenic detection of NaOCl in solution. Also, we have constructed a colorimetric test paper for visual sensing of NaOCl. Furthermore, fluorescence imaging studies indicated that probe 1 was a versatile tool for in vitro imaging of NaOCl in living cells. Thus, to the best of our knowledge, probe 1 represents one of the rare examples of multimode responsive ClO- probes.

Eu(III)-DO3A and BODIPY dyad as a chemosensor for anthrax biomarker.

The sensitive and selective determination of Bacillus anthracis spores before the infection is vital for human health and safety. Dipicolinic acid (DPA) is an excellent biomarker due to its presence in the nucleus of bacterial spores at high concentrations (up to 1 M, about 15% dry weight). In the present work, a new molecular chemosensor 1, based on europium(III)-DO3A and BODIPY dyad, is developed to detect DPA in phosphate-buffered saline (PBS) buffered solution and tap water samples. Also, 1 can be used as a ratiometric optical chemosensor to track DPA.