RESUMO
Neonicotinoid insecticides specifically target insect subtypes of nicotinic acetylcholine receptors. Acetamiprid (ACE: C10H11ClN4), the neonicotinoid insecticide, is used to control crop insect pests worldwide. It is a nitrile, monochloropyridine, and carboxamidine that is highly soluble and accessible to waterways. There, it causes neurotoxic and oxidative perturbance to non-target organisms. The unionid mussel Chamabradia rubens is a common Northern River Nile suspension feeder. The current study aimed to assess ACE filtration from waters by C. rubens, and whether this biological power can reduce ACE effects on fish. Removal of ACE by C. rubens was assessed using LC-MS/MS. Zebrafish Danio rerio adults were exposed to different sublethal doses of ACE in the presence or absence of C. rubens in their aquaria. The results showed that mussels could remove significant ACE amounts from water, where it accumulated mostly in the digestive gland. The presence of C.rubens in zebrafish aquaria having ACE was accompanied by significant upregulation of antioxidant enzyme gene transcripts and total H2O2 scavenging, in contrast to mussel-free ACE-exposed groups. Meanwhile, liver triglycerides rose 5-6-fold in response to ACE in the "Fish-Only" groups, indicating an ACE-induced hepatotoxicity. Also, Insulin-like growth factor 1 (igf1) and fish body mass increased more in "Fish + Mussel" groups than in the "Fish-Only" ones. In aggregate, these findings suggest that the Nile mussel could reduce the oxidative stress and metabolic changes induced in fish by ACE. This can contribute valuable environmental and economic benefits upon the use of this mussel as a biofilter.
Assuntos
Inseticidas , Neonicotinoides , Poluentes Químicos da Água , Peixe-Zebra , Animais , Peixe-Zebra/fisiologia , Neonicotinoides/toxicidade , Inseticidas/toxicidade , Poluentes Químicos da Água/toxicidade , FiltraçãoRESUMO
The competition impact and feedstock type on the removal of water pesticides using biochar have not yet been sufficiently investigated. Therefore, here we investigated the potentiality of three different biochars (BCs) derived from rice husk (RHB), date pit (DPB), and sugarcane bagasse (SBB) biowastes for the simultaneous removal of ten pesticides from water in a competitive adsorption system. The BCs structural characterization and morphology were investigated by XRD, FTIR spectroscopy and SEM analysis. The potential adsorption mechanisms have been investigated using various isothermal and kinetic models. RHB showed the highest removal percentages (61% for atrazine/dimethoate and 97.6% for diuron/chlorfenvinphos) followed by DPB (56% for atrazine/dimethoate and 95.4% for diuron/chlorpyrifos) and then SBB (60.8% for atrazine/dimethoate and 90.8% for chlorpyrifos/malathion). The higher adsorption capacity of RHB and DPB than SBB can be due to their high total pore volume and specific surface area (SSA). Langmuir model described well the sorption data (R2 = 0.99). Adsorption equilibrium was achieved after 60 min for RHB, and 120 min for both DPB and SBB. The optimum adsorbent dose (g/L) was 10 for RHB and 4 for DPB and SBB. The removal efficiency of pesticides was enhanced by decreasing pH from 9 to 5 by RHB and to 3 by DPB and SBB. XRD and FTIR spectroscopy confirmed that BCs contain some active adsorption groups and metal oxides such as MgO, SiO, Al2O3, CaO, and TiO2 that can play an effective role in the pesticides sorption. BET-N2 adsorption analysis demonstrated that the BC pore size contributes significantly to pesticide adsorption. These findings indicate that RHB, DPB, and SBB have ability for adsorption of water pesticides even under acidic conditions. Therefore, the rice husk, date pit, and sugarcane bagasse biowastes could be pyrolyzed and reused as effective and low-cost sorbents for elimination of hazardous substances such as pesticides in the aqueous environments.