Percepción y prioridades respecto al proceso de consentimiento informado quirúrgico; un análisis mediante métodos mixtos

Objetivo: Identificar las principales preocupaciones antes y después de realizarse un procedimiento quirúrgico, e identificar diferencias basadas en género, edad y nivel de escolaridad. Material y Método: Mediante un muestreo no probabilístico incidental se aplicaron 201 encuestas semiestructuradas a personas en la sala de espera del HSJD. Las respuestas fueron procesadas mediante un análisis mixto donde se organizaron categorías por unidades de significancia y se ordenaron de forma decreciente para luego comparar según edad, género y escolaridad. Resultados: Respecto a los aspectos que las personas consideran relevante conocer previo a la cirugía, 4 categorías concentran el 71 % del total: 1. Riesgos, complicaciones y secuelas, 2. Explicación del procedimiento, 3. Beneficios y pronóstico, 4. Cuidados y evaluaciones previas. En relación con lo que le gustaría que le explicaran después, se prioriza: 1. Conocer los resultados del procedimiento (19,3%), 2. Control, derivaciones y cuidados paliativos (15%), 3. Tiempo de recuperación (13,7%). Diferencias significativas: una mayor necesidad de acompañamiento y explicación de la patología expresada por hombres, el interés por conocer y los aspectos relacionados con funcionalidad postoperatoria en personas entre 18-65 años, el interés de conocer los cuidados y evaluaciones previas, conocer los resultados del procedimiento, los cuidados de la herida y la funcionalidad posoperatoria según nivel educacional. Discusión y conclusión: Existen variaciones significativas en contenido prioritario según edad, género y nivel educacional. Finalmente, el presente artículo proporciona evidencia en cuanto a las principales prioridades de pacientes en el sistema público chileno para el consentimiento informado.

Aim: To identify the main concerns before and after undergoing a surgical procedure, and to identify differences based on gender, age and level of education. Material and Method: 201 semi-structured surveys were administered to people in the waiting room of the HSJD by means of incidental non-probabilistic sampling. The responses were processed by means of a mixed analysis in which categories were organized by units of significance and ordered in decreasing order to then compare according to age, gender and schooling. Results: Regarding the aspects that people considered relevant to know before surgery, 4 categories accounted for 71% of the total: 1. Risks, complications and sequels, 2. Explanation of the procedure, 3. Benefits and prognosis, 4. Prior care and evaluations. In relation to what you would like to have explained to you afterwards, priority is given to: 1. knowing the results of the procedure (19.3%), 2. Monitoring, referrals and palliative care (15%), 3. Recovery time (13.7%). Significant differences: a greater need for accompaniment and explanation of the pathology expressed by men, interest in knowing and aspects related to postoperative functionality in persons between 18-65 years of age, interest in knowing the previous care and evaluations, knowing the results of the procedure, wound care and postoperative functionality according to educational level. Discussion and conclusion: There are significant variations in priority content according to age, gender and educational level. Finally, the present article provides evidence regarding the main priorities of patients in the chilean public system for informed consent.

Surface modification of TiO2/ZnO nanoparticles by organic acids with enhanced methylene blue and rhodamine B dye adsorption properties.

The United Nations Organization (UNO) has revealed that approximately 2.1 billion people do not have access to treated water. Methylene blue (MB) and rhodamine B are produced as water pollutants in textile, plastic, and dye industries. In this study, oxalic acid or lactic acid surface-modification were applied to TiO2/ZnO nanoparticles aiming to improve antibacterial and adsorption properties. The mixtures containing the corresponding acid and nanoparticles in 0.25 : 1/0.5 : 1 ratios of ZnO and TiO2 correspondingly were subjected to ultrasonic treatment with a catenoidal ultrasonic probe coupled to a homemade ultrasonic generator with an output power of 750 W, wave amplitude of 50% and variable frequency in the range of 15-50 kHz. To verify the influence of the ultrasonic treatment, different treatment times of 30, 45, 60, and 90 min were applied. Unmodified and modified TiO2/ZnO nanoparticles were characterized by FTIR, TGA, XRD, SEM, and XPS. From the results, obtained from the physicochemical characterization, in the ZTO90 and ZTL90 samples a greater modification was shown. The SEM images showed that a coating was present on the surface of the ceramic particles of the ZTL90 sample. The O 1s deconvolution in the XPS spectra indicates a greater presence of C[double bond, length as m-dash]O bonds in the ZTL90 sample. In parallel, the sample ZTL90 presented 85 and 89% adsorption efficiency for MB and rhodamine B dyes in a time of 12 min, and important antibacterial activity against E. coli and S. epidermis could be evidenced.

Agromorphological characterization of cacao (Theobroma cacao L.) accessions from the germplasm bank of the National Institute of Agrarian Innovation, Peru.

Agromorphological characterization of cacao accessions in Peru is currently an important tool in the conservation and genetic improvement of cacao germplasm. The objective of this study was to carry out the morphological and agronomic characterization of 113 cacao accessions from the Huarangopampa germplasm bank. Tree, leaf, flower, fruit and seed descriptors were used. The data collected were processed by descriptive statistics using multivariate techniques. Five groups were formed according to similar characteristics. The accessions of group 1 are vigorous trees with an pod index of 19.27 pods/kg of seeds; the groups that presented better differential characteristics were group 2 with erect tree architecture, intermediate vigorousness, purple seed color and pod index of 20.07 pods/kg of seeds and group 3, which had the highest number of accessions with the lowest pod index of 18.77 pods/kg of seeds, besides being vigorous trees and having purple seeds. On the other hand, group 4 presented a particular characteristic of white seed color and high pod indexes with 22.11 pods/kg of seeds. Finally, group 5 accessions were characterized by intermediate tree architecture and vigor with an pod index of 21.3 pods/kg of seeds. The morphoagronomic characterization constitutes a first advance in the identification of cacaos with potential for genetic improvement and advances in the Peruvian chocolate industry.

Characterization of Coffea arabica L. parent plants and physicochemical properties of associated soils, Peru.

It is important to carry out the morphological characterization of coffee parent plants and the physicochemical properties of the associated soils in the Amazon region, Peru, in order to achieve germplasm conservation. One hundred coffee mother plants were identified and located in five provinces of the region and evaluated according to morphological descriptors such as stipula shape, young leaf color, leaf shape, leaf apex shape, young shoot color, leaf color, fruit color, fruit shape, mature leaf color, and rust incidence percentage. In the plots where the parent plants were located, soil sampling was carried out to determine the physical and chemical properties. The varieties with the greatest presence in the five provinces were Típica and caturra roja, with the greatest number of specimens reported for the province of Bagua. The predominant stipule shape was triangular (91%), lanceolate leaf shape (60%) and red fruit color (90%). Bongará reported the lowest incidence of yellow rust, as well as the Mundo Novo Rojo variety. Soil pH ranged from acidic to neutral values, low electrical conductivity, high organic matter content, low phosphorus content, high potassium levels and medium cation exchange capacity. The predominant textural class was sandy loam. The physical and chemical characterization of the soils under study show favorable ranges to encourage the best development of coffee cultivation.

Complex interactions between nicotine and resveratrol in the Drosophila melanogaster wing spot test.

Nicotine (NIC) and resveratrol (RES) are chemicals in tobacco and wine, respectively, that are widely consumed concurrently worldwide. NIC is an alkaloid known to be toxic, addictive and to produce oxidative stress, while RES is thought of as an antioxidant with putative health benefits. Oxidative stress can induce genotoxic damage, yet few studies have examined whether NIC is genotoxic in vivo. In vitro studies have shown that RES can ameliorate deleterious effects of NIC. However, RES has been reported to have both antioxidant and pro-oxidant effects, and an in vivo study reported that 0.011 mM RES was genotoxic. We used the Drosophila melanogaster wing spot test to determine whether NIC and RES, first individually and then in combination, were genotoxic and/or altered the cell division. We hypothesized that RES would modulate NIC's effects. NIC was genotoxic in the standard (ST) cross in a concentration-independent manner, but not genotoxic in the high bioactivation (HB) cross. RES was not genotoxic in either the ST or HB cross at the concentrations tested. We discovered a complex interaction between NIC and RES. Depending on concentration, RES was protective of NIC's genotoxic damage, RES had no interaction with NIC, or RES had an additive or synergistic effect, increasing NIC's genotoxic damage. Most NIC, RES, and NIC/RES combinations tested altered the cell division in the ST and HB crosses. Because we used the ST and HB crosses, we demonstrated that genotoxicity and cell division alterations were modulated by the xenobiotic metabolism. These results provide evidence of NIC's genotoxicity in vivo at specific concentrations. Moreover, NIC's genotoxicity can be modulated by its interaction with RES in a complex manner, in which their interaction can lead to either increasing NIC's damage or protecting against it.

Nanocomposite PLA/C20A Nanoclay by Ultrasound-Assisted Melt Extrusion for Adsorption of Uremic Toxins and Methylene Blue Dye.

Design of functional materials it is of great importance to address important problems in the areas of health and environment. In the present work, the synthesis and application of poly-meric nanocomposite materials with poly (lactic acid) (PLA) and modified nanoclay (cloisite 20A) with 1,4-diaminobutane dihydrochloride at different reaction times were studied. The concentra-tions of the nanoclays in the PLA matrix were 0.5, 1 and, 5% by weight (wt%). TGA showed that sample C20AM 120 (120 min of treatment) obtained the highest degree of modification considering the weight losses of the analyzed samples. An FT-IR signal at 1443 cm-1 suggests that the organic modifier is intercalated between the galleries of the clay. XRD, SEM and XPS suggest good disper-sion at low concentrations of the nanoclay. Adsorption tests revealed that the highest percentage of removal of uremic toxins and methylene blue was the sample with 5% wt/wt chemically modified nanoclay, suggesting good affinity between the modified nanoclays in the PLA matrix with the nitrogenous compounds.

Non-Woven Fabrics Based on Nanocomposite Nylon 6/ZnO Obtained by Ultrasound-Assisted Extrusion for Improved Antimicrobial and Adsorption Methylene Blue Dye Properties.

Approximately 200,000 tons of water contaminated with dyes are discharged into effluents annually, which in addition to infectious diseases constitute problems that afflict the population worldwide. This study evaluated the mechanical properties, surface structure, antimicrobial performance, and methylene blue dye-contaminant adsorption using the non-woven fabrics manufactured by melt-blowing. The non-woven fabrics are composed of nylon 6 (Ny 6) and zinc oxide nanoparticles (ZnO NPs). The polymer nanocomposites were previously fabricated using variable frequency ultrasound assisted-melt-extrusion to be used in melt-blowing. Energy dispersion spectroscopy (SEM-EDS) images showed a homogeneous dispersion of the ZnO nanoparticles in nylon 6. The mechanical properties of the composites increased by adding ZnO compared to the nylon 6 matrix, and sample Ny/ZnO 0.5 showed the best mechanical performance. All fabric samples exhibited antimicrobial activity against S. aureus and fungus C. albicans, and the incorporation of ZnO nanoparticles significantly improved this property compared to pure nylon 6. The absorption efficiency of methylene blue (MB), during 60 min, for the samples Ny/ZnO 0.05 and Ny/ZnO 0.25 wt%, were 93% and 65%, respectively. The adsorption equilibrium data obeyed the Langmuir isotherm.

Male urethritis. A review of the ideal diagnostic method. / Uretritis masculina. Una revisión del método ideal de diagnóstico.

Male urethritis is an inflammation of the urethra and the periurethral glands; it is widely classified as gonococcal or non-gonococcal. The most frequent microorganisms responsible are Chlamydia trachomatis, Neisseria gonorrhoeae, Mycoplasma genitalium, Mycoplasma hominis, Ureaplasma urealyticum, and Ureaplasma parvum. In the last three decades, the diagnosis of sexually transmitted diseases depended almost exclusively on traditional methods, such as culture, enzyme immunoassay, fluorescent antibody staining, and hybridization, until the appearance of molecular techniques. Clinical syndromes such as urethritis are rarely specific for a single microorganism, so screening strategies should allow multiple agents to be considered. Multiplex PCR is the fastest and most sensitive technique for the diagnosis of gonococcal and non-gonococcal urethritis. Male urethritis without treatment is one of the main health problems related to reproductive and sexual function, constituting one of the main causes of infertility. The objective of this mini-review was to analyze the epidemiology, causes, diagnosis, and complications of male urethritis.

Effectiveness of a multimodal training programme to improve general practitioners' burnout, job satisfaction and psychological well-being.

BACKGROUND: The changes in the models of care for mental disorders towards a community focus and deinstitutionalisation might have risen General practitioners' (GPs) workload, increasing their mental health concerns and the need for solutions. Pragmatic research into improving GPs' work-related health and psychological well-being is limited by focusing mainly on stressors and through not providing systematic attention to the development of positive mental health via interventions that develop psychological resources and capacities. The aim of this study was twofold: a) to determine the effectiveness of an intensive multimodal training programme for GPs designed to improve their management of mental-health patients; and b) to ascertain if the program could be also useful to improve the GPs management of their own burnout, job satisfaction and psychological well-being. METHOD: Eighteen GPs constituted a control group that underwent the routine clinical Mental health support programme for primary care. An experimental group (N = 20) additionally received a Multimodal training programme (MTP) with an Integrated Brief Systemic Therapy (IBST) approach. Through questionnaires and a clinical interview, level of burnout, professional satisfaction, psychopathological state and various indicators of the quality of administrative and healthcare management were analysed at baseline and 10 months after the programme. RESULTS: In relation to government of mental-health patients indicators, on the one hand MTP group showed statistically significant improvements in certain administrative health parameters, but on the other it did not improve opinions and attitudes towards mental illness. Regarding GPs management of their own burnout, job satisfaction and psychological well-being assessments, the MTP presented better scores on global psychopathological state and better evolution of satisfaction at work; psychopharmacology use dropped in both groups; in contrast, the MTP did not improve burnout levels. CONCLUSIONS: Findings of this preliminary study are promising for the MTP (with an IBST approach) practice in primary care. More research evidence is required from larger samples and randomized controlled trials to support both the hypothetical adoption of MTP (with an IBST approach) as a part of a continuing professional-training programme for GPs' management of mental-health patients and its positive effects on work-related health factors.

Sanitization of Chicken Frames by a Combination of Hydrogen Peroxide and UV Light To Reduce Contamination of Derived Edible Products.

Chicken carcass frames are used to obtain mechanically separated chicken (MSC) for use in other further processed food products. Previous foodborne disease outbreaks involving Salmonella-contaminated MSC have demonstrated the potential for the human pathogen to be transmitted to consumers via MSC. The current study evaluated the efficacy of multiple treatments applied to the surfaces of chicken carcass frames to reduce microbial loads on noninoculated frames and frames inoculated with a cocktail of Salmonella enterica serovar Enteritidis and Salmonella enterica serovar Typhimurium. Inoculated or noninoculated frames were left untreated (control) or were subjected to treatment using a prototype sanitization apparatus. Treatments consisted of (i) a sterile water rinse, (ii) a water rinse followed by 5 s of UV-C light application, or (iii) an advanced oxidation process (AOP) combining 5 or 7% (v/v) hydrogen peroxide (H2O2) with UV-C light. Treatment with 7% H2O2 and UV-C light reduced numbers of aerobic bacteria by up to 1.5 log CFU per frame (P < 0.05); reductions in aerobic bacteria subjected to other treatments did not statistically differ from one another (initial mean load on nontreated frames: 3.6 ± 0.1 log CFU per frame). Salmonella numbers (mean load on inoculated, nontreated control was 5.6 ± 0.2 log CFU per frame) were maximally reduced by AOP application in comparison with other treatments. No difference in Salmonella reductions obtained by 5% H2O2 (1.1 log CFU per frame) was detected compared with that obtained following 7% H2O2 use (1.0 log CFU per frame). The AOP treatment for sanitization of chicken carcass frames reduces microbial contamination on chicken carcass frames that are subsequently used for manufacture of MSC.

Graphene Nanoplatelets Modified with Amino-Groups by Ultrasonic Radiation of Variable Frequency for Potential Adsorption of Uremic Toxins.

Chronic kidney disease (CKD) is a worldwide public health problem. In stages III and IV of CKD, uremic toxins must be removed from the patient by absorption, through a treatment commonly called hemodialysis. Aiming to improve the absorption of uremic toxins, we have studied its absorption in chemically modified graphene nanoplatelets (GNPs). This study involved the reaction between GNPs and diamines with reaction times of 30, 45 and 60 min using ultrasound waves of different amplitudes and frequencies. Functionalized GNPs were analyzed by Fourier Fourier-transform infrared spectroscopy (FTIR), X-ray photoelectron spectroscopy (XPS), Scanning electron microscopy and energy dispersitive spectroscopy (SEM-EDS), and Thermogravimetric analysis (TGA). The analysis of the functional groups confirmed the presence of amide and hydroxyl groups on the surface of the GNPs by reactions of diamines with carboxylic acids and epoxides. Adsorption of uremic toxins was determined using equilibrium isotherms, where the maximum percentage of removal of uremic toxins was 97%. Dispersion of modified graphene nanoplatelets was evaluated in water, ethanol and hexane, as a result of this treatment was achieved a good and effective dispersion of diamines-modified graphene nanoplatelets in ethanol and hexane. Finally, the results of hemolysis assays of the modified graphene with amine demonstrated that it was not cytotoxic when using 500 mg/mL. The samples of modified graphene demonstrated low degree of hemolysis (<2%), so this material can be used for in vivo applications such as hemodialysis.

Melt-Mixed Thermoplastic Nanocomposite Containing Carbon Nanotubes and Titanium Dioxide for Flame Retardancy Applications.

The study of polymeric nanocomposites is a possible alternative to conventional flame retardants. The aim of the present work is to investigate the effects of carbon-nanotubes (CNT) and TiO2 nanoparticles (NPs) on the thermo-mechanical, flammability, and electrical properties of polypropylene (PP). In this work, PP-TiO2/CNT nanocomposites were obtained with TiO2/CNT mixtures (ratio 1:2) through the melt extrusion process, with different weight percentage of nanoparticles (1, 5, and 10 wt %). The PP-TiO2/CNT nanocomposites were characterized by DSC, TGA, MFI, FTIR, XRD, and SEM. It was possible to determine that the thermal stability of the PP increases when increasing the content of NPs. A contrary situation is observed in the degree of crystallinity and thermo-oxidative degradation, which decreased with respect to pure PP. The TiO2 NPs undergo coalition and increase their size at a lower viscosity of the nanocomposite (1 and 5 wt %). The mechanical properties decreased slightly, however, the Young's modulus presented an improvement of 10% as well as electrical conductivity, this behavior was noted in nanocomposites of 10 wt % of NPs. Flammability properties were measured with a cone calorimeter, and a reduction in the peak heat release rate was observed in nanocomposites with contents of nanoparticles of 5 and 10 wt.

Biofilm microbial composition changes due to different surface chemical modifications of activated carbon cloths in the biotransformation of 4-nitrophenol.

Activated carbon cloths (ACCs) were used as biofilms supports in the anaerobic biotransformation of 4-nitrophenol (4NP). As received ACC material (AW) was oxidized with HNO3 (OX) and then functionalized with anthraquinone-2,6-disulfonate (AQ). The three ACCs were packed in hybrid UASB reactors and seeded with anaerobic granular sludge for biotransformation experiments. The results indicated that ACC-packed bioreactors improved the biotransformation of 4NP by twofold as compared to the control reactor without support materials. However, the biotransformation effciency of AW, OX and AQ was very similar (59%), indicating the role of ACC as biofilm support and not as redox mediator. After 4NP biotransformation several physicochemical and biological changes were observed like (1) the point of zero charge (pHPZC) shift from acidic values (AW = 5.0, OX = 3.4, AQ = 3.1) to neutral values (pHPZC = 7.6 on average), (2) increase in the concentration of acidic and basic surface functional groups over ACC materials and the amount of supported biomass on ACCs due to biofilm formation, and (3) enrichment of exoelectrogenic microorganisms belonging to the genera Geobacter over carbonyl-rich ACC surface as revealed by 16S rRNA amplicon sequencing. Overall, the results suggest that chemical modifications of ACCs changed the microbial composition of the biofilm, but the higher concentration of carbonyl groups on ACC did not affect the biotransformation of 4NP.

Surface Modification of Graphene Nanoplatelets by Organic Acids and Ultrasonic Radiation for Enhance Uremic Toxins Adsorption.

Ultrasound energy is a green and economically viable alternative to conventional techniques for surface modification of materials. The main benefits of this technique are the decrease of processing time and the amount of energy used. In this work, graphene nanoplatelets were treated with organic acids under ultrasonic radiation of 350 W at different times (30 and 60 min) aiming to modify their surface with functional acid groups and to improve the adsorption of uremic toxins. The modified graphene nanoplatelets were characterized by Fourier transform infrared spectroscopy (FT⁻IR), thermogravimetric analysis (TGA), and X-ray photoelectron spectroscopy (XPS). The optimum time for modification with organic acids was 30 min. The modified nanoplatelets were tested as adsorbent material for uremic toxins using the equilibrium isotherms where the adsorption isotherm of urea was adjusted for the Langmuir model. From the solution, 75% of uremic toxins were removed and absorbed by the modified nanoplatelets.

Establishing a model for childhood obesity in adolescent pigs.

OBJECTIVE: Rising worldwide prevalence of obesity and metabolic diseases in children has accentuated the importance of developing prevention and management strategies. The objective of this study was to establish a model for childhood obesity using high-fat feeding of adolescent pigs, as pigs have a longer developmental period and are physiologically more similar to humans than rodents. METHODS: Crossbred pigs were fed a high-fat diet (HFD) or low-fat diet (n = 6/treatment) from postnatal day 49 to 84. On postnatal day 84, an oral glucose tolerance test was performed, jugular blood sampled to determine lipopolysaccharide levels and plasma lipids, intestinal digesta collected to characterize microbial and metabolite composition and back fat and intestinal tissue assayed for gene expression. RESULTS: Five-week HFD increased weight gain and back fat thickness, caused dyslipidaemia and impaired glucose tolerance and increased expression of genes in back fat suggesting inflammation. HFD pigs had distinct proximal colon microbiota with 48% reduction (P < 0.05) in Bacteroidetes and increased expression of pro-inflammatory genes interleukin-18 and tumour necrosis factor in ileum (P < 0.05). CONCLUSIONS: These findings indicate that adolescent pigs should be considered a suitable model for childhood obesity, because short-term HFD feeding is sufficient to induce obesity and glucose intolerance, recapitulating disease characteristics in adolescent pigs.

Evaluation of different dietary alterations in their ability to mitigate the incidence and severity of woody breast and white striping in commercial male broilers.

The following study was conducted to define how multiple nutritional strategies affect broiler performance, meat yield, and the presence and severity of white striping (WS) and woody breast (WB) in high-yielding broilers. Relative to a commercial set of reference broiler diets (Commercial reference diet; Trt 1) that were fed in a 4-phase program, the following nutritional strategies were investigated: increasing the ratio of digestible arginine: digestible lysine (dArg: dLys ranged from 113 to 126; Trt 2), supplementing Trt 1 with 94.4 mg vitamin C/kg feed (Trt 3), doubling the vitamin pack inclusion rate (Trt 4), reducing the digestible amino acid density (dAA) of only the grower phase by 15% and feeding the same Trt 1 starter, finisher, and withdraw diets (Trt 5), and combining the 4 strategies just mentioned (Trt 6). There was no difference in performance at the end of the starter phase (P = 0.066); however, at the end of the grower and finisher phases, feeding lower dAA grower diets suppressed BW (Trts 5 and 6; P < 0.001) and increased FCR. Differences in performance amongst all treatments disappeared at day 49 (P = 0.220). No differences were observed in average breast weight (P = 0.188); however, breast yield (as a % of live weight) was greatest for Trt 1 and least for Trt 6 (P = 0.041). The WB score dropped from 1.83 in Trt 1 to 1.49, 1.27, 1.74, 1.53, and 1.43 in treatments 2 to 6, respectively (P = 0.018). These changes were the result of a shift in WB score, where the WB class that contained scores of 2 and 3 shifted from 61.3% in Trt 1 to 49.3, 35.9, 60.0, 50.8, and 38.7 in treatments 2 to 6, respectively. Given the FCR, breast weight data and the fact that high WB scores result in a devaluation of breast meat, feeding a higher ratio of dArg: dLys, higher vitamin C, or lower dAA in the grower phase results in better breast meat quality and value.

Buckling down on torus fractures: has evolving evidence affected practice?

PURPOSE: The purpose of this study was to evaluate the management of paediatric torus fractures of the distal forearm in current practice in light of growing evidence supporting a 'minimalist' approach with splint immobilization and limited follow-up. We hypothesized that 'traditional' cast-based management has persisted despite alternative evidence. METHODS: A retrospective review was performed of a consecutive series of paediatric patients diagnosed with torus fractures of the distal forearm between 2011 and 2014. Records were reviewed to abstract the type of immobilization (splint versus cast) prescribed at each visit, number of radiographic exams, duration of immobilization, number of clinical visits and complications. The primary outcome was the proportion of patients exclusively managed in splints. Injuries were grouped based on treatment into a cast group (CG) and a splint group (SG) for statistical analyses. Additionally, injuries were grouped by epoch of time to determine if immobilization usage patterns evolved. RESULTS: A total of 240 forty injuries met criteria for inclusion. Of these, 16 (6.7%) were exclusively splinted (SG). Relative to the CG, the SG had fewer clinical visits (p < 0.001), fewer radiographic exams (p < 0.001) and a shorter total encounter time (p = 0.015). No change in immobilization use occurred over the study period. In all, 21 (9.4%) of the CG experienced complications. No clinically significant displacements occurred in either group. CONCLUSION: Cast utilization and frequent radiographic follow-up remain prevalent at our institution in the management of paediatric torus fractures. Splint-only management was associated with fewer clinical visits, fewer radiographic exams and a shorter total encounter time. LEVEL OF EVIDENCE LEVEL III: Therapeutic retrospective cohort study.

Descriptive sensory and instrumental texture profile analysis of woody breast in marinated chicken.

The broiler industry is currently experiencing a muscle anomaly referred to as "woody breast," and the effect of different cooking methods on the marination properties of severe woody breast (SWB) has not yet been reported. This study compared the texture attributes of marinated (injected) normal (NOR) and SWB using a convection oven and a flat-top grill. The objectives were 1) to develop and validate a descriptive texture attribute panel with 6 trained panelists using a 16-point scale and 2) to evaluate the instrumental texture profile analysis (TPA) using a texture analyzer. Sixty-four NOR and SWB were obtained from a commercial facility. Fillet color (L*, a*, b*) and pH were measured before marination. In each of 2 trials, the breast muscles were injected in bulk with 15% brine (0.48 STPP, 0.55% NaCl, final concentration), and marinade retention was determined after 20 minutes. The meat was vacuum packaged, stored at -20°C (7 d sensory; 29 d TPA) and then thawed (4°C, 24 h). Fillets were cooked to 73°C on a flat-top grill (176°C) or in an oven (176°C), and cook loss % was determined. Panelist samples (2 × 2 cm) and TPA samples (4 × 2 cm) were cut into 3 pieces. Color and pH were higher for SWB than NOR fillets (P < 0.05). Marinade retention was 83.21% for NOR meat and 59.23% for SWB meat. The flat-top grill method resulted in higher cook loss than oven (P < 0.05). SWB had higher cook loss when compared to NOR (P < 0.05). Sensory texture descriptors springiness, hardness, denseness, cohesiveness, fracturability, fibrousness, crunchiness, and chewiness were higher for SWB than NOR fillets (P < 0.05). TPA attributes also showed higher values for SWB compared to NOR (P < 0.05). No differences in texture were found between the grill and oven for sensory and TPA attributes. In summary, marinated SWB has significant texture differences when compared to NOR, regardless of cooking method.

Utilizing original XPC™ in feed to reduce stress susceptibility of broilers.

Reduction of stress is an important factor in improving poultry welfare, especially during periods of heat stress. A study was conducted to evaluate the effects of feeding the functional metabolites of Diamond V Original XPCTM to broilers reared under ambient or heat stress temperatures. Dietary treatments included: control feed (CON) and Original XPC fed continuously at 1.25 kg/MT (XPC). Half the birds in each dietary treatment were subjected to either no heat stress (24°C constant) or heat stress (35°C:24°C for 18:6 h daily) from 28 to 42 d. At the end of the heat stress period, blood was collected from 40 birds/treatment. Blood was analyzed for plasma corticosterone (CORT), plasma HSP70 (HSP70), and heterophil/lymphocyte ratios (H/L). At 42 d, bilateral metatarsal traits were also measured in 40 birds/treatment to assess physical asymmetry. Birds fed XPC had significantly lower CORT levels than CON (P < 0.001; 5,129 ± 617 vs. 8,433 ± 730, respectively). Physical asymmetry scores were also significantly higher in CON compared to XPC fed broilers (P < 0.001; 1.50 ± 0.13 vs. 0.54 ± 0.05, respectively). H/L ratios were significantly greater in CON than for XPC birds (P = 0.01; 0.81 ± 0.05 mm vs. 0.62 ± 0.05 mm, respectively). No differences were observed between CON and XPC fed broilers in HSP70. However, heat stress did increase (P < 0.0001) HSP70 compared to no heat stress birds (5.65 ± 0.12 vs. 4.78 ± 0.11 pg/mL, for heat stress and no heat stress, respectively). Feeding XPC to broiler chickens improved animal welfare via reduced stress indicators while under heat stress or no heat stress conditions. These results indicate that feeding XPC may improve poultry welfare by reducing heat stress susceptibility.