Comparison of cardiorespiratory parameters between 6-minute walk test and 1-minute sit to stand test in young adults with post-COVID-19: follow-up 3 months.

Background: The investigation of cardiorespiratory fitness in young adults post-coronavirus disease 2019 (COVID-19) is interesting because this information may help in understanding cardiorespiratory function in these populations. Moreover, it helps to know that these impairments possibly interfere with study, learning, and the activities of daily life in young adults post-COVID-19. This study aims to investigate and compare the cardiorespiratory parameters between 6-minute walk test (6MWT) and 1-minute sit-to-stand test (1-min-STST) in healthy young adults and post-COVID-19 and at a 3-month follow-up. Methods: Forty-six young adults were recruited and divided into two groups including healthy young adults in one group (n=23) and post-COVID-19 patients in the other group (n=23). The young adults were assessed for cardiorespiratory parameters including heart rate (HR), systolic blood pressure (SBP), diastolic blood pressure (DBP), pulse oxygen saturation (SpO2), rate of perceived exertion (RPE), and leg fatigue before and after performing a 6MWT and a 1-min STST at baseline and the 3-month follow-up. Test sequences were randomly assigned using the website randomizer.org. Results: Post-COVID-19 had significantly decreased post-HR, post-SBP, post-SpO2, post-RPE, post-leg fatigue, and increased the distance of 6MWT, and number of steps of 1-min-STST when compared with the baseline (P<0.05). However, all parameters of cardiorespiratory could recover and return to the values of healthy young adults by the follow-up at 3 months. Conclusions: Post-COVID-19 who recovered from mild-COVID-19 for about 6 months recovered their cardiorespiratory parameters to the values of healthy young adults.

Evaluating the Balancing Ability of Older Adults with Diabetes Mellitus Using the Multi-directional Reach Test.

BACKGROUND: The incidence of elderly people experiencing falls is currently increasing, which results in serious medical issues, such as fear of falling, limited physical activity, disability, and bone fractures, especially hip fractures. This study aimed to investigate balancing ability using a multi-directional reach test (MDRT) in older adults with and without diabetes mellitus. METHODS: 72 older adults with and without diabetes mellitus were recruited, and divided into two groups, including older adults without diabetes mellitus (n = 36) and older adults with diabetes mellitus (n = 36). All subjects completed all directions of the MDRT. RESULTS: There were no significant differences in MDRT scores in all directions between the two groups (p>0.05). Both groups achieved the highest MDRT scores in the forward direction. In contrast, the two groups had the lowest scores of MDRT in a backward direction. Furthermore, older adults with diabetes mellitus had lower MDRT scores in all directions than older adults without diabetes mellitus. CONCLUSION: The current study indicated that MDRT could be used to investigate the ability of balance in individuals with diabetes mellitus.

The Different Body Mass Index Does Not Impact Physical Health in Older Adults without Sedentary Behavior: A Cross-Sectional Study.

AIMS AND OBJECTIVE: This study aimed to compare and determine the correlation of BMI on physical health, including muscle strength, balance, and cardiorespiratory fitness in older adults without sedentary behavior. METHODS: A total of 65 older adults without sedentary behavior were recruited and divided into 3 groups on the basis of body mass index (BMI): normal weight (n= 22/group), overweight (n= 22/- group), and obese (n= 21/group). All the older adults were evaluated for upper limb muscle strength using the handgrip strength test, lower limb muscle strength using the sit-to-stand (STS10) test, balance ability using the time up and go (TUG) test, and cardiorespiratory fitness using the 6-minute walk test (6MWT). RESULTS: The handgrip strength test, STS10 test, and distance of 6MWT showed no difference among the groups (p> 0.05). However, overweight group had a significantly longer duration for the TUG test when compared to normal weight, while obese group had a significantly longer duration for the TUG test when compared with overweight and normal weight groups (p< 0.01). In addition, BMI was positively correlated with the duration of the TUG test. Whereas, the handgrip strength test, STS10 test, and distance of 6MWT were not correlated with BMI. CONCLUSION: The BMI does not impact upper and lower limb muscle strength or the cardiorespiratory fitness test, except for the ability to balance in older adults without sedentary behavior.

Lower extremity muscle strength equation of older adults assessed by Five Time Sit to Stand Test (FTSST).

Background: The decline in lower limb muscle strength, one of the risk factors for falling in the older adults, puts older persons at an increased risk of falling. The assessment of the lower limb muscle strength is very important. Objective: The purpose of this study was to construct the equation for predicting knee extensor muscle strength based on demographic data and the results of the Five-Time Sit-to-Stand Test (FTSST). Methods: A total of 121 healthy elders (mean age 68.00±7.26) were asked to complete the FTSST and submit the demographic information. By using a stationary push-pull dynamometer, the knee extensor strength of each participant was assessed. The multiple regression analysis was used to explore knee extensor strength prediction equation. Results: The findings demonstrated that the knee extensor strength equation was developed using variables obtained from gender, weight, and time to complete the FTSST. The equation was found to have a high correlation (r=0.838) and 70.1% estimation power. Its formula was as follows: Knee extensor strength=32.735+3.688 (gender; female=0 or male=1) + 0.189 (weight) - 2.617 (time to complete the FTSST). However, there was an estimating error in this equation of 4.72 kg. Conclusion: The determining factors influencing knee extensor strength, which can be utilized to estimate the strength in elderly individuals, are demographic variables including gender, weight, and the time taken to complete the FTSST.

Predicting Age of Independent Walking in Preterm Infants: A Longitudinal Study Using Neonatal Characteristics and Motor Development Variables.

OBJECTIVE: To formulate an equation estimating months to independent walking in moderate to late preterm infants based on neonatal characteristics and gross motor development from 7 months to independent walking. METHODS: Sixty infants born between 32 to 36 weeks were assessed using Alberta Infant Motor Scale (AIMS) for gross motor development. Neonatal characteristics were recorded at 7 months, and caregiver-reported independent walking onset. Pearson correlation analyzed age, AIMS scores, and neonatal factors. Multiple regression developed the prediction equation. RESULTS: The equation for independent walking onset, which included gestational age (GA) at birth, total AIMS score at 10 months of age (10th AIMS), and birth head circumference (BHC), exhibited a strong correlation (r=0.707) and had a predictive power of 50.0%. The equation is as follows: age onset of independent walking (months)=33.157, -0.296 (GA), -0.132 (10th AIMS), -0.196 (BHC), with an estimation error of 0.631 months. CONCLUSION: Neonatal characteristics, such as GA, 10th AIMS, and BHC, are key determinants in estimating the onset of independent walking in moderate to late preterm infants.

Overweight in elderly increases postural instability during sit-to-stand test: a Kinect-based assessment.

BACKGROUND: Being overweight increases the risk of falls and subsequent injuries. However, conventional functional balance tests may not be appropriate for this population as body weight can affect test performance. Thus, it is crucial to develop reliable methods for assessing balance ability in overweight individuals. AIM: The purpose of this study was to utilize a portable gaming device (Kinect Xbox 360) to measure the center of mass (CoM) of elderly individuals and compare the results between normal weight and overweight elderly women. METHODS: The study included 64 female elderly individuals who were divided into two groups: 32 normal weight and 32 overweight subjects, matched for age (matched pairs design). The study collected the body's CoM during the five-time sit-to-stand test (FTSST) using Kinect camera. Additionally, the participants underwent the time up and go test (TUGT) and one-leg standing balance (OLSB) tests. The Kinect Xbox 360 demonstrated high test-retest reliability for measuring body's center of mass sway, with ICC3,1 value of 0.982 in the vertical direction, 0.983 in the mediolateral (ML) direction, and 0.997 in the anteroposterior (AP) direction. Additionally, the technical error of measurement (TEM) was very low at 0.006, 0.002, and 0.004, respectively. The % coefficient of variation ranged from 1.31% to 5.68%, indicating good measurement consistency. RESULTS: Overweight individuals took longer to complete FTSST and TUGT compared to normal weight individuals, but no significant difference was observed in OLSB tests. Moreover, overweight elderly individuals had greater sway length in the ML and AP directions compared to normal weight elderly individuals. DISCUSSION AND CONCLUSION: Overweight elderly individuals had poorer balance than their normal weight counterparts when using Kinect camera. The researchers suggest that Kinect device can be a cost-effective alternative to measure balance performance among overweight elderly in the community-based setting.

Balance Ability and Quality of Life in Older Adult with Recovery from Mild COVID-19.

BACKGROUND: In this study, we aimed to assess the ability to balance and quality of life (QoL) among older adults without a history of coronavirus disease 2019 (COVID-19) and those who had recovered from mild COVID-19. METHODS: We recruited 80 older adults and categorized them into the following two groups based on their history of COVID-19: those without COVID-19 (n=40) and those who had recovered from mild COVID-19 (n=40). We assessed the participants' ability to balance using the multi-directional reach test and timed up and go (TUG) test, and evaluated their QoL using the Short Form-36. RESULTS: Compared with older adults without a history of COVID-19, those who had recovered from mild COVID-19 demonstrated no differences in the scores of the forward, backward, right, and left directions (p>0.05), but a significantly longer duration for the TUG test (p=0.02) and a reduced QoL. CONCLUSION: Our study results demonstrated decreased ability to balance and poor QoL among older adults who had recovered from mild COVID-19.

Predictive Ability of the 2-Minute Step Test for Functional Fitness in Older Individuals with Hypertension.

BACKGROUND: The 2-minute step test (2MST) is a simple and inexpensive functional test that measures an individual's ability to perform continuous stepping up and down on a step platform for two minutes. This study evaluated the 2MST as a tool for assessing functional fitness in older individuals with hypertension and determined the correlation between the 2MST and physical fitness tests. METHODS: A total of 91 older individuals with hypertension performed physical fitness tests, including the 2MST, 6-minute walk test (6MWT), five times sit-to-stand test (FTSST), grip strength and leg strength assessments, and timed up and go test (TUG) to collectively assess their physical fitness. RESULTS: A cutoff score of ≤60 steps in the 2MST had 87.50% sensitivity and 70.59% specificity in predicting functional exercise performance in older individuals with hypertension. Additionally, the number of steps in the 2MST was positively correlated with the distance covered in the 6MWT, isometric grip strength, and isometric leg strength and negatively correlated with the duration of the FTSST and TUG. CONCLUSIONS: A cutoff score of ≤60 steps in the 2MST predicted functional exercise performance in older individuals with hypertension with 87.50% sensitivity and 70.59% specificity and was correlated with other physical fitness tests, suggesting that the 2MST is a useful tool for assessing functional exercise performance.

The Influence of Knee Extensor and Ankle Plantar Flexor Strength on Single-Leg Standing Balance in Older Women.

Impaired balance is a significant risk factor for falls among older adults. The precise impact of lower-extremity muscles, including the proportion of muscle strength, on the performance of single-leg standing balance tests in older individuals is very interesting. The aim of this study is to examine the correlation between the knee extensor (KE), ankle plantar flexor (AP) muscle strength, and performance in single-leg standing balance tests in older females. Additionally, it aims to evaluate the combined proportion of KE and AP muscle strength in maintaining balance during single-leg standing. A total of 90 older females (mean age 67.83 ± 8.00 years) were recruited. All participants underwent maximum voluntary isometric contraction (MVIC) testing of the KE and AP muscles, as well as single-leg standing balance tests with eyes open (SSEO) and eyes closed (SSEC). To examine the influence of KE and AP muscle strength on balance performance, multiple regression analysis was conducted. Low correlations were found between SSEO and MVIC of KE and AP muscles, but moderate correlations were found with percentage of MVIC to body weight ratio (%MVIC/BW). The best model for SSEO included 0.99 times of the %MVIC/BW of AP and 0.66 times that of KE muscles as independent predictor variables (r = 0.682). In conclusion, AP muscle strength was found to have a greater impact on single-leg standing balance compared with KE muscle strength.

Comparison of the Ability of Static and Dynamic Balance Tests to Determine the Risk of Falls among Older Community-Dwelling Individuals.

This study aimed to examine the validity of balance tests and compare their diagnostic accuracy to determine the risk of falls among older community-dwelling individuals. Eighty-five older participants were assessed based on their demographics and fall data. They were then assessed for the ability to perform balance measures, including five times sit-to-stand tests (FTSSTs), timed up and go tests (TUGs), three times stand and walk tests (TTSWs), functional reach tests (FRTs), and single-leg stance tests (SLSs). The correlation between fall data and balance measures was found to be significant for all parameters (p < 0.05). The TTSW showed the highest level of ability to indicate the risk of falls among older community-dwelling adults with sensitivity = 92.68%, specificity = 84.09%, and AUC = 0.931 (95%CI = 0.860 to 1.000). These findings confirm the benefits of the practical functional balance measures to determine the risk of falls among older community-dwelling individuals.

Association between Stress and Physical Fitness of University Students Post-COVID-19 Pandemic.

Post-COVID-19 pandemic, most universities changed their educational model from online courses to onsite learning, allowing students to attend regular face-to-face classes. These changes can cause stress in students, which affects physical fitness. The aim of this study was to investigate the relationship between stress levels and physical fitness in female university students. The participants were 101 female university students, 18-23 years of age. All participants completed the Suan Prung Stress Test-60 (SPST-60). The physical fitness test included body composition, cardiorespiratory fitness, as well as musculoskeletal fitness. Multiple linear regression analysis was used to determine the associations between SPST-60 scores and physical fitness. A p-value < 0.05 was considered statistically significant. We found a negative correlation between the sources of stress scores, here environment, and maximal oxygen consumption (ß = -0.291; 95% CI, -0.551, -0.031). We also found that symptoms of stress scores in the parasympathetic and sympathetic nervous systems were positively associated with waist-hip circumference ratio (WHR) (ß = 0.010; 95% CI, 0.002, 0.017 and ß = 0.006; 95% CI, 0.000, 0.012, respectively). Moreover, the symptoms of stress, here emotion, were positively associated with the WHR (ß = 0.005; 95 %CI, 0.001, 0.009) and negatively associated with upper extremity muscle strength (ß = -0.005; 95% CI, -0.009, 0.000). The results of this study confirmed the associations between stress levels in the post-COVID-19 pandemic era and WHR, maximal oxygen consumption, and upper extremity muscle strength. As a result, stress reduction or prevention alternatives should be considered in order to maintain physical fitness and prevent stress disorders.

Impacts of surgical mask wearing on cardiopulmonary fitness using a six-minute walk test among older adults with hypertension.

Background: This study aims to investigate the impacts of surgical mask wearing on cardiopulmonary fitness using a six-minute walk test (6MWT) among older adults with hypertension. Methods: The 45 older adults with hypertension volunteers were recruited and randomized into a no mask wearing condition and a surgical mask wearing condition. All volunteers were investigated through submaximal exercise testing using a 6MWT. Cardiopulmonary parameters were measured before and after performing a 6MWT. Results: The results indicate post performance on the 6MWT for both the with and without surgical mask conditions significantly increased systolic blood pressure, heart rate, respiratory rate, and oxygen saturation (SpO2) when compared to before performing a 6MWT. However, surgical masks resulted in no differences to systolic blood pressure, diastolic blood pressure, heart rate, respiratory rate, SpO2, and 6MWT distance when compared to no masks. In contrast, the surgical mask condition significantly increased perceived exertion when compared with the no mask condition (p < 0.05). Conclusion: This study indicates that surgical masks did not have an impact on cardiopulmonary fitness in older adults with hypertension.

Effects of Wearing Face Masks on Cardiorespiratory Parameters at Rest and after Performing the Six-Minute Walk Test in Older Adults.

The effects of wearing cloth masks and surgical masks were investigated on respiratory rate, heart rate, blood pressure, oxygen saturation and perceived exertion at rest and after performing a six-minute walk test (6MWT) in older adults. Forty older adults were recruited and randomized into six groups including wearing no mask, cloth masks and surgical masks, at rest and during the 6MWT. At rest, all subjects sat quietly wearing no mask, a cloth mask or a surgical mask. All subjects performed a 6MWT by walking as fast as possible without running while wearing no mask, a cloth mask or a surgical mask. Respiratory rate, heart rate, blood pressure, oxygen saturation and perceived exertion were assessed before and after the rest and the 6MWT. Results showed that cloth masks and surgical masks did not impact cardiorespiratory parameters at rest or after performing a 6MWT, while an increase in perceived exertion was apparent in the groups wearing surgical masks and cloth masks after performing the 6MWT (p < 0.01). Cloth masks and surgical masks did not have an impact on cardiorespiratory fitness at rest and after performing the 6MWT in older adults.

PCSK9 inhibitor effectively attenuates cardiometabolic impairment in obese-insulin resistant rats.

Long-term high-fat diet consumption causes obese-insulin resistance and cardiac mitochondrial dysfunction, leading to impaired left ventricular (LV) function. Atorvastatin effectively improved lipid profiles in obese patients. However, inadequate reduction in low density lipoprotein cholesterol (LDL-C) level was found. Proprotein convertase subtilisin/kexin type 9 (PCSK9) inhibitor effectively reduced LDL-C levels. We hypothesized that this PCSK9 inhibitor has a greater efficacy in attenuating cardiometabolic impairments than atorvastatin in obese-insulin resistant rats. Female rats were fed with either a high fat or normal diet for 12 weeks. High fat diet fed rats (HFD) were then divided into 3 groups and were given vehicle, atorvastatin (40 mg/kg/day; s.c.), or PCSK9 inhibitor (4 mg/kg/day; s.c.) for additional 3 weeks. The metabolic parameters, cardiac and mitochondrial function and [Ca2+]i transients were determined. HFD rats developed obese-insulin resistance as indicated by increased plasma insulin and HOMA index. Although high-fat diet fed rats treated with vehicle (HFV) rats had markedly impaired LV function as indicated by reduced %LVFS, impaired cardiac mitochondrial function, and [Ca2+]i transient regulation, these impairments were attenuated in high-fat diet fed rats treated with atorvastatin (HFA) and high-fat diet fed rats treated with PCSK9 inhibitor (HFP) rats. However, these improvements were greater in HFP rats than HFA rats. Our findings indicated that the PCSK9 inhibitor exerted greater cardioprotection than atorvastatin through improved mitochondrial function in obese-insulin resistant rats.

PCSK9 inhibitor and atorvastatin reduce cardiac impairment in ovariectomized prediabetic rats via improved mitochondrial function and Ca2+ regulation.

Post-menopausal women have a higher risk of developing cardiometabolic dysfunction. Atorvastatin attenuates dyslipidaemia and cardiac dysfunction but it can have undesirable effects including increased risk of diabetes and myalgia. Currently, the proprotein convertase subtilisin/kexin type 9 (PCSK9) inhibitor efficiently reduces low-density lipoprotein cholesterol (LDL-C) levels more effectively than atorvastatin. We have been suggested that PCSK9 inhibitor attenuated cardiometabolic impairment more effectively than atorvastatin in ovariectomized prediabetic rats. Female Wistar rats (n = 48) were fed a normal diet (ND) or high-fat diet (HFD) for 12 weeks. Then, HFD rats were assigned to a sham-operated (Sham) or ovariectomized (OVX) group. Six weeks after surgery, the OVX group was subdivided into 4 treatment groups: vehicle (HFOV), atorvastatin (HFOA) (40 mg/kg/day; s.c.), PCSK9 inhibitor (HFOP) (4 mg/kg/day; s.c.) and oestrogen (HFOE2 ) (50 µg/kg/day; s.c.) for an additional 3 weeks. Metabolic parameters, cardiac and mitochondrial function, and [Ca2+ ]i transients were evaluated. All HFD rats became obese-insulin resistant. HFS rats had significantly impaired left ventricular (LV) function, cardiac mitochondrial function and [Ca2+ ]i transient dysregulation. Oestrogen deprivation (HFOV) aggravated all of these impairments. Our findings indicated that the atorvastatin, PCSK9 inhibitor and oestrogen shared similar efficacy in the attenuation in cardiometabolic impairment in ovariectomized prediabetic rats.

A proprotein convertase subtilisin/kexin type 9 inhibitor provides comparable efficacy with lower detriment than statins on mitochondria of oxidative muscle of obese estrogen-deprived rats.

OBJECTIVES: The aim of the study was to compare the effects of atorvastatin, a proprotein convertase subtilisin/kexin type 9 inhibitor (PCSK9i), and 17ß-estradiol on oxidative muscle mitochondria in a model of menopause with obesity. METHODS: Female Wistar rats consumed either a standard diet (n = 12) or a high-fat/calorie diet (HFCD: n = 60). At week 13, standard diet-fed rats underwent a sham operation, whereas HFCD-fed rats underwent either a sham operation (n = 12) or an ovariectomy (n = 48). At week 19, all sham-operated rats received vehicle, and ovariectomized HFCD-fed rats received either vehicle, 40 mg/kg/d of atorvastatin, 4 mg/kg/d of PCSK9i (SBC-115076), or 50 µg/kg/d of 17ß-estradiol for 3 weeks (n = 12/group). Metabolic parameters and soleus muscle physiology were investigated at the end of week 21. RESULTS: Sham-operated and ovariectomized HFCD-fed rats developed obesity, hyperlipidemia, and insulin resistance, also showing increased oxidative phosphorylation (OXPHOS) proteins, ratio of p-Drp1-to-total Drp1 protein, malondialdehyde level, mitochondrial reactive oxygen species, and mitochondrial membrane depolarization in soleus muscle. All drugs equally decreased insulin resistance, OXPHOS proteins, ratio of p-Drp1-to-total Drp1 protein, and malondialdehyde level in soleus muscle. Only atorvastatin and PCSK9i attenuated hypertriglyceridemia, whereas 17ß-estradiol had greater efficacy in preventing weight gain than the other two drugs. In addition, 17ß-estradiol decreased mitochondrial reactive oxygen species and mitochondrial membrane depolarization. Atorvastatin increased ratio of cleaved caspase 3,8-to-procaspase 3,8, and cytochrome C. CONCLUSIONS: 17ß-Estradiol exhibits the greatest efficacy on the attenuation of obesity with the least harmful effect on skeletal muscle in a model of menopause with obesity, yet its effect on the treatment of hyperlipidemia is inferior to those of standard lipid-lowering agents.

Proprotein convertase subtilisin/kexin type 9 (PCSK9) inhibitor exerts greater efficacy than atorvastatin on improvement of brain function and cognition in obese rats.

The accumulation of lipid as a result of long-term consumption of a high-fat diet (HFD) may lead to metabolic and brain dysfunction. Atorvastatin, a recommended first-line lipid-lowering agent, has shown beneficial effects on metabolic and brain functions in several models. Recently, proprotein convertase subtilisin/kexin type 9 (PCSK9) inhibitor was approved as an effective therapeutic drug for dyslipidemia patients. However, few studies have reported on the effect of this PCSK9 inhibitor on brain function. In addition, the comparative efficacy on the improvement of metabolic and brain functions between PCSK9 inhibitor and atorvastatin in obese models have not been elucidated. We hypothesized that PCSK9 inhibitor improves metabolic and brain functions in an obese model to a greater extent than atorvastatin. Thirty-two female rats were fed with either a normal diet (ND) or HFD for 15 weeks. At week 13, ND rats were given normal saline and HFD rats were given either normal saline, atorvastatin (40 mg/kg/day) or PCSK9 inhibitor (4 mg/kg/day) for 3 weeks. Oxidative stress, blood brain barrier breakdown, microglial hyperactivity, synaptic dysplasticity, apoptosis, amyloid proteins production in the hippocampus and cognitive decline were found in HFD-fed rats. Atorvastatin and PCSK9 inhibitor therapies equally attenuated hippocampal apoptosis and amyloid protein production in HFD-fed rats. Interestingly, PCSK9 inhibitor had the greater efficacy than atorvastatin on the amelioration of hippocampal oxidative stress, blood brain barrier breakdown, microglial hyperactivity, synaptic dysplasticity in the hippocampus and cognitive decline. These findings suggest that PCSK9 inhibitor may be another drug of choice for improving brain function in the obese condition with discontinued statin therapy.

The comparative effects of high dose atorvastatin and proprotein convertase subtilisin/kexin type 9 inhibitor on the mitochondria of oxidative muscle fibers in obese-insulin resistant female rats.

The present study aimed to compare the effects of high dose atorvastatin and a proprotein convertase subtilisin/kexin type 9 (PCSK9) inhibitor on the mitochondrial function in oxidative muscle fibers in obese female rats. Female Wistar rats were fed with either a normal diet (ND: n = 12) or a high-fat diet (HFD: n = 36) for a total of 15 weeks. At week 13, ND-fed rats received a vehicle, and HFD-fed rats were divided to three groups to receive either a vehicle, 40 mg/kg/day of atorvastatin, or 4 mg/kg/day of PCSK9 inhibitor (SBC-115076) for 3 weeks. Soleus muscles were investigated to assess mitochondrial ROS, membrane potential, swelling, mitochondrial-related protein expression, and level of malondialdehyde (MDA). The results showed that HFD-fed rats with vehicle developed obese-insulin resistance and dyslipidemia. Both atorvastatin and PCSK9 inhibitor reduced obesity and dyslipidemia, as well as improved insulin sensitivity in HFD-fed rats. However, the efficacy of PCSK9 inhibitor to increase weight loss and reduce dyslipidemia in HFD-fed rats was greater than those of atorvastatin. An increase in MDA level, ratio of p-Drp1ser616/total Drp1 protein, CPT1 protein, mitochondrial ROS, and membrane depolarization in the soleus muscle were observed in HFD-fed rats with vehicle. PCSK9 inhibitor enabled the restoration of all these parameters to normal levels. However, atorvastatin facilitated restoration of some parameters, including MDA level, p-Drp1ser616/total Drp1 ratio, and CPT1 protein expression. These findings suggest that PCSK9 inhibitor is superior to atorvastatin in instigating weight loss, cholesterol reduction, and attenuation of mitochondrial oxidative stress in oxidative muscle fibers of obese female rats.

DNA damage and adverse neurological outcomes among garlic farmers exposed to organophosphate pesticides.

Garlic farmers used organophosphate pesticides to control insects, resulting in an increased risk for adverse health outcomes. Thus, we investigated exposure to organophosphate pesticides, DNA damage, nerve conduction, and neurological symptoms among 134 garlic farmers. They were interviewed, measured nerve conduction, and collected blood and urine for determining DNA damage and dialkylphosphate metabolites. Total dialkylphosphate levels of farmers who began cultivating garlic in October were significantly higher than those of non-farmers and the farmers who began cultivating in November and December. Farmers showed significantly longer comet tail lengths and tail moments than non-farmers. However, their mean compound motor and sensory nerve action potential amplitudes of the median, ulnar, and common peroneal nerves were within normal ranges. Measurement of DNA damage is useful as a biomarker of long-term and low-level exposure to organophosphate pesticides; however, electromyography might be not sufficiently sensitive to detect nerve conduction effects in farmers exposed to pesticides.

The effects of proprotein convertase subtilisin/kexin type 9 inhibitors on lipid metabolism and cardiovascular function.

Low density lipoprotein cholesterol (LDL-C) is a well-established risk factor for cardiovascular disease. Although there are several developed lipid lowering drugs such as statins and fenofibrates, many patients do not achieve an adequate response. Recently, proprotein convertase subtilisin/kexin type 9 (PCSK9) inhibitors have been developed as a new therapeutic strategy for cholesterol regulation. PCSK9 binds to low density lipoprotein receptors (LDLR) and initiates LDLR degradation, elevating LDL-C. Therefore, PCSK9 inhibition could exert beneficial effects on cardiovascular disease outcomes. This review comprehensively summarizes and discusses the effects of PCSK9 inhibitors on lipid metabolism and cardiovascular function comparatively with current lipid lowering drugs. This review also details essential information regarding the cardiovascular benefits of PCSK9 inhibition which could encourage further clinical studies.