[Humeral fracture due to arm wrestling - an indirect fracture in a doped athlete]. / Oberarmschaftfraktur beim Armdrücken - ein indirekter Bruch bei einem gedopten Sportler.

BACKGROUND: Humeral shaft fractures due to recreational arm wrestling are reported from time to time. CASE REPORT: We report the case of a 19 year old, anabolic steroids up taking, recreational arm wrestler who sustained a spiral fracture of the right humeral bone and secondary radial nerve palsy. We discuss the surgical treatment and the biomechanics of this rare injury mechanism. CONCLUSION: Every professional or recreational arm wrestler has to be aware of dangerous sports injuries.

Investigations on prophylactic effects of coarse feed structure and/or potassium diformate on the microflora in the digestive tract of weaned piglets experimentally infected with Salmonella Derby.

The purpose of this study was to examine potential prophylactic effects of a coarse feed structure and/or potassium diformate (KDF as feed additive) addition to diets on the microflora of the digestive tract in reared piglets experimentally infected with Salmonella Derby. The results show that coarse grinding as well as KDF addition are able to influence positively the intestinal flora and are capable to reduce Salmonella excretion of infected piglets. Coarse grinding of main ingredients (e.g. cereals) led to an increased number of lactobacilli as well as to higher counts of Gram-positive coccoid bacteria in the colon chyme, while KDF resulted in a tendency towards lower counts of Escherichia coli within the digestive tract. Moreover, a combination of both treatments influenced the composition of the gastrointestinal flora quite positively. Furthermore, the combination of these dietetic measures resulted in a reduced Salmonella excretion rate, shorter Salmonella shedding period and a reduced translocation of Salmonella within the infected piglets. The positive effects of combining both treatments led to a significantly reduced spreading of Salmonella within the group of pigs, which might be used to diminish Salmonella prevalence in pig production.

[The prevalence of Actinobaculum suis in boars of breeding herds in the Omsk region (Russian Federation) by indirect immunofluorescence technique]. / Untersuchung zur Verbreitung von Actinobaculum suis unter den Ebern in den Schweinezuchtbetrieben des Gebiets Omsk (Russische Föderation) unter Verwendung der indirekten Immunofluoreszenz-Technik.

Actinobaculum suis (Corynebacterium suis, Eubacterium suis, Actinomyces suis) was detected in the preputial diverticulum of 64,8% of 162 boars investigated in 8 districts of the region Omsk (Russian Federation) by indirect immunofluorescent technique. Until yet no informations were available about the prevalence of Actinobaculum (A.) suis in swine herds of the Russian Federation. The study shows that A. suis, as a main aetiological factor of cystitis and pyelonephritis in sows, is widely spread among the boars of the region Omsk. Prevalence of A. suis was not influenced by housing conditions, age or breed of investigated boars. Indirect immunofluorescent technique for detection of A. suis provides a good method for screening investigations with high numbers of samples.

[Chronic-idiopathic enteropathy in cats--a case control study]. / Die chronisch-idiopathische Enteropathie bei der Katze--Eine Fall-Kontroll-Studie.

Aim of this study is to clinically, microbiologically and histopathologically characterize inflammatory bowel disease (IBD) in the cat. Nine cats with chronic persistent or intermitent vomitus and diarrhea were examined between 1998 and 2001. All cats had a thuorough diagnostic workup performed. Full thickness biopsies from stomach, duodenum, jejunum, ileum and colon were surgically obtained for histopathological examination. Duodenal juice was obtained by direct aspiration for microbiologic qualitative and semiquantitative examination. Seven cats euthanized for other medical reasons were used as controls. Six cats had a lymphoplasmacytic IBD and three an eosinophilic IBD. Four cats with IBD had additional diseases diagnosed. Three cats with IBD had elevated bacterial counts. Retrospectively no correlation could be found between clinical symptoms and histopathological results. Serum TLI was not able to differentiate chronic pancreatitis and IBD. Serum folic acid and cobalamin did not correlate with the distribution of lesions in the gastrointestinal tract. Finally, no correlation was found between bacterial counts in the proximal duodenum and IBD.

Laparoscopic-endoscopic rendezvous resection of gastric tumors.

BACKGROUND: Submucosal and mucosal tumors of the stomach display a wide spectrum of histopathologic and prognostic characteristics. Biopsies obtained using endoscopic techniques often do not provide the representative histologic sample needed for further therapeutic decisions. METHODS: From 1999 to 2002, 18 patients with gastric tumors underwent a combined endoscopic-laparoscopic local resection of the tumors using two different procedures and were prospectively analyzed. Tumors of the posterior wall were resected using laparoscopic intragastral resection (LIR). Tumors located in the anterior wall were resected using lesion-lifting or the laparoscopic wedge resection (LWR) approach. RESULTS: Laparoscopic resections were performed in 18 patients. The mean age of the patients was 64.4 years (range, 38-81 years). Preoperative preparation included endoscopy with biopsies and histologic examination, ultrasound examination, computed tomography scan, and endoscopic ultrasonography. We performed the LWR on 10 patients and the LIR on 8 patients. After resection, the final immunohistologic examination of the specimens showed gastrointestinal stroma cell tumors in nine cases, neurinomas or benign neurofibrotic tumors in four cases, and one leiomyoma. Four additional patients with mucosal early gastric cancer and high comorbidity risks also underwent a limited full-thickness wedge resection. In all the patients, the surgical margins were tumor free, and no lymphatic or venous invasion was encountered in pathologic specimens. Method-specific complications occurred in one case (perforation of the stomach wall). No fatal outcome had to be registered. CONCLUSIONS: When selected properly, the laparoscopic-endoscopic approach is considered to be curative and minimally invasive for resection of localized gastric tumors. In cases of histopathologically unknown tumors preoperatively, definitive examination of the complete specimen provides the basis for further therapeutic decisions.

[Diagnosis of swine dysentery and spirochaetal diarrhea. III. Results of cultural and biochemical differentiation of intestinal Brachyspira species by routine culture from 1997 to 1999]. / Zur Diagnostik von Schweinedysenterie und Spirochaetendiarrhoe. 3. Mitteilung: Ergebnisse kulturell-biochemischer Differenzierung intestinaler Brachyspiren in der Routinediagnostik der Jahre 1997 bis 1999.

A survey is given on the occurrence and distribution of different Brachyspira species in pigs, in the northwest of Germany. In total 2975 specimen (feces, fecal swabs, colon) were taken and sent for laboratory analysis during the years 1997 to 1999. 1218 Brachyspira (B.) strains were found by cultural analysis. 1757 samples (59%) were negative. The cultural and biochemical differentiation revealed 720 (59.1%) strains B. hyodysenteriae (77.5% were indole negative), 22 (1.8%) B. pilosicoli, 29 (2.4%) B. intermedia, 167 (3.7%) B. innocens and 114 (9.4%) B. murdochii. 166 (13.6%) strains could not be identified. These strains could either not be compared with any of the described species by the methods used or it was impossible to achieve a pure culture from these isolates. The results demonstrate the wide spread of B. hyodysenteriae in pig herds in the northwest of Germany with a very high prevalence of indole negative strains. The most frequent strain was B. hyodysenteriae. B. pilosicoli which causes spirochaetal diarrhoea was rarely isolated and seems not to play an important role in Germany. Experience from routine cultures for Brachyspira give evidence that it is more useful to examine faeces from single pigs instead of pooled samples from a herd. It is recommended to use special transport media for the transport of the specimen.

[Investigations on Brachyspira--diagnostic and therapeutic strategies in swine dysentery]. / Untersuchungen zur Brachyspira--Diagnostik und Behandlungsstrategie bei der Schweinedysenterie.

The infectious agent of swine dysentery, Brachyspira (Br.) hyodysenteriae, seems to be widespread in German pig herds. Due to different reasons the eradication is increasingly difficult. Not only the success of therapeutic procedures but also the possibilities of diagnostics are unsatisfactory. Although only the bacteriological investigation of faeces or intestinal probes by culture techniques allows the typing of Brachyspira strains and the testing of drug resistance, however, the rate of false negative results is relatively high. In comparison with the cultural method an easy, prompt and cheap immunofluorescent test (IFT) resulted in a good sensitivity (90%). The higher rate of negative results by culture techniques can not be attributed to a lower specificity of the IFT, but to an insufficient transport of samples to the laboratory. The IFT therefore has to be considered as a valuable supplement to the cultural diagnostic of Br. hyodysenteriae. It is absolutely necessary to establish strategies in eradication of swine dysentery which result in pig breeding herds free of Br. hyodysenteriae. Only weaner pigs which are reliable free of this germ guarantee a fattening period sufficiently free of swine dysentery. The principles of different measures in effective eradication are described.

[Diagnosis of swine dysentery and spirochaetal diarrhea. 2. Effort of macrorestriction analysis for differentiation of intestinal Serpulina relative to determination by culture-biochemical markers following species classification]. / Zur Diagnostik von Schweinedysenterie und Spirochätendiarrhoe.

Genotypic differentiation by means of macrorestriction fragment profile analysis using Mlul restriction enzyme was carried out differentiating 41 Serpulina field strains from swine (38), dog (2) and a rat as well as ten type and reference strains into 40 electrophoretic types. A dendrogram was created using the average linkage between groups method. At a level of 50% similarity the patterns could be divided into six groups that roughly corresponded to the results yielded by cultural and biochemical methods formerly (FELTRUP et al. 1999). Five of these clusters corresponded to the five known porcine Serpulina species, one cluster contained the S. pilosicoli isolates from dog and rat included in this study. Interestingly all nine investigated indole negative, strongly haemolytic isolates were clustered together in one group with the S. hyodysenteriae strains, so that incidence of indole negative variants of S. hyodysenteriae was confirmed. Because of being grouped together with two S. intermedia isolates, the suitability of B 256 as S. innocens type strain is--in accord to investigations carried out by PETTERSSON et al (1996)--called in question.

[The diagnosis of swine dysentery and spirochaete diarrhea. 1. Cultural-biochemical differentiation of intestinal Serpulina in routine diagnosis]. / Zur Diagnostik von Schweinedysenterie und Spirochätendiarrhoe. 1. Mitteilung: Kulturell-biochemische Differenzierung intestinaler Serpulinen in der Routinediagnostik.

Frequent incidence of Serpulina strains showing all cultural and biochemical characteristics of Serpulina (S.) hyodysenteriae except of being indole negative, and alpha-galactosidase positive isolates showing strong haemolysis on Columbia agar with 5% sheep blood and trypticase soy agar with 5% ox blood, respectively, was the cause to evaluate common biochemical and cultural methods in Serpulina routine diagnostics. To this purpose ten type and reference strains as well as 47 field strains were examined for their ability to produce indole, haemolysis, hippurate cleavage, alpha-galactosidase, alpha- and beta-glucosidase activity. Two four-hour identification-systems were used, RapID ANA II and Rosco diagnostic tablets. The ability to produce indole was determined by different methods. All investigations were carried out at least two times. For the investigation of haemolytic patterns trypticase soy agar with 10% ox blood proved to be most effective. Results received using this agar could always be confirmed by the ring phenomenon. Determining the ability to produce indole by adding p-dimethylaminocinnamaldehyde to bacterial growth collected on a cotton swab was confirmed to be more sensitive than other methods. Both four-hour-systems were shown to be useful in Serpulina diagnostics, though in the RapID ANA II only four of 18 available reactions could be used and the hippurate cleavage reaction has to be carried out additionally. Using cultural and biochemical methods, it was possible to assign the type and reference strains to the correct species, as well as 46 of 47 field isolates could be identified including all five known intestinal Serpulina species from swine. 27 strains were determined as S. hyodysenteriae, nine of these isolates atypically being indole negative. In contrast one canine S. pilosicoli strain was atypical showing indole production. Therefore incidence of indole negative variants of S. hyodysenteriae as well as indole positive S. pilosicoli isolates must be taken into consideration.

[Investigations into the occurrence and the antibiotic susceptibility of gram negative anaerobes of the genera Bacteroides, Prevotella, Porphyromonas and Fusobacterium in specimens obtained from diseased animals]. / Untersuchungen zum Vorkommen und zur Antibiotikaresistenz gramnegativer Anaerobier der Gattungen Bacteroides, Prevotella, Porphyromonas und Fusobacterium im Untersuchungsmaterial erkrankter Tiere.

From different samples of 247 diseased animals (cattle, sheep, goat, horse, pig, dog, cat, rodent, zoo-animals), 410 strains of gram-negative anaerobes were cultured. 297 isolates (72.4%) could be differentiated to the species level by using cultural-biochemical methods, gaschromatography and cell-wall-lipidanalysis. They belonged to 29 different species. For an additional 113 strains (27.6%) only the genus could be determined. Bacteria belonging to the genus Fusobacterium occurred with the highest isolation rates (36% of all strains) in the samples examined, followed by Bacteroides spp. (26.1%), Prevotella spp. (19.9%) and Prophyromonas spp. (17.8%). Fusobacterium necrophorum was the single species isolated most frequently. Antibiotic susceptibility tests by E-test were performed on 100 strains belonging to the the above mentioned genera. Of these strains 18% were resistant to penicillin and 20% to tetracycline. The resistant strains belonged mainly to the Bacteroides fragilis-group. Resistant rates to most other antimicrobial agents tested were Amoxicillin in combination with clavulanic acid: 1%, Chloramphenicol: 3%, Clindamycin:8%. All 100 selected strains proved to be susceptible to Metronidazol.

[Demonstration of pneumonia in swine as a constant problem: culture and immunofluorescence microscopic studies of bronchioalveolar lavage (BAL) and serological findings]. / Nachweis von Pneumonieerregern bei Schweinen aus Problembeständen: Kulturelle und immunfluoreszenzmikroskopische Untersuchungen der bronchoalveolären Lavage (BAL) und serologische Befunde.

Bronchoalveolar lavage was performed in 128 pigs from five fattening units showing acute pneumonia (48 animals), subclinical purulent pneumonia (17 animals), and chronic purulent pneumonia (63 animals). These samples were investigated for bacteria. Additionally immunofluorescence microscopy as well as serological investigations were performed to detect antibodies against several bacteria and viruses. Pasteurella multocida could be detected in more than a half of the samples of pigs with acute pneumonia. Bordetella bronchiseptica and mycoplasmas were isolated in a lower amount. Probably these bacteria infected the pigs of at least one herd after a primary infection with influenza virus because (i) influenza virus could be detected in three of four animals investigated for influenza virus by culture methods, (ii) the virus could be detected in one third of the animals investigated for by immunofluorescence microscopy, and (iii) antibodies against influenza virus could be detected in almost all animals. From pigs with subclinical purulent pneumonia Bordetella bronchiseptica as the only bacterial lung pathogen could be isolated exclusively from nearly each sample. From the samples of pig suffering from chronic purulent pneumonia first of all Bordetella bronchiseptica, Pasteurella multocida and different mycoplasma species could be detected. Using cultural methods Actinobacillus pleuropneumoniae could be isolated from six samples only, in contrast to frequent positive reactions against Actinobacillus pleuropneumoniae antigens obtained by immunofluorescence microscopy and CFT.

[Serologic examinations for the detection of antibodies against Eubacterium suis in swine]. / Serologische Untersuchungen zum Nachweis von Antikörpern gegen Eubacterium suis beim Schwein.

Determination of antibodies against Eubacterium suis (E. suis) in the serum of pigs was carried out by an indirect immunofluorescence test. Therefore 35 sows with urinary tract infections as well as 43 healthy control sows were investigated. E. suis could be detected in the urine of 19 sows. Serological results were compared with the bacteriological examinations. To reach a specificity of 100% for detection of animals actually infected with E. suis, titres from 1:16++ can be accepted as positive. Therefore sensitivity gave a level of 78.9%. Seroconversion and development of antibody titers were studied on 15 sows, which were experimentally infected with E. suis. Antibodies could be shown 3 weeks after infection at the earliest, they were preferably demonstrated in sows with a haemorrhagic cystitis. No correlation existed between the affection of the kidneys and the development and the level of titres respectively. Serological investigations can turn out negative in spite of E. suis infection, if immunological response still has not taken place or development of antibodies has failed to appear after short-time infection of the bladder.

Characterization of Staphylococcus intermedius from healthy dogs and cases of superficial pyoderma by DNA restriction endonuclease patterns.

In order to study the possible clonal relation of Staphyloccocus (S.) intermedius from canine superficial pyoderma and from healthy carriers, isolates from pustular swabs and from vaginal, nasal and normal skin sabs were typed using macrorestriction analysis with Sma I and pulsed-field gel electrophoresis. From the size of the resulting fragments the size of the chromosome of S. intermedius could be determined to be roughly 1500 +/- 200 kb on the average. The fingerprints were very heterogeneous though characteristically distinct from patterns of (human) S. aureus as published by others. Strains from superficial pyoderma were not found to be more similar to each other than strains from healthy carriers. Therefore it was concluded that strains from this type of skin infection probably did not belong to a certain subpopulation of S. intermedius, which might have indicated a higher virulence of these strains.

[The occurrence and significance of enterotoxin-producing Clostridium perfringens strains in the intestinal tract of horses]. / Untersuchungen zum Vorkommen und zur Bedeutung von enterotoxinbildenden Clostridium-perfringens-Stämmen im Darmkanal von Pferden.

100 faecal samples from clinically healthy horses of different age groups and feeding habits, 50 samples of faeces from horses suffering from enteropathy accompanied by diarrhoea and small and/or large intestine from 25 horses that had died after an intestinal disease were examined for the presence of Clostridium (Cl.) perfringens. The frequency with which Cl. perfringens was detected was 22% in clinically healthy horses, 32% in horses with diarrhoea and 52% in the dead horses. In two faecal samples from the horses with diarrhoea the microbial count of Cl. perfringens was ca. 10(6) cfu/g faeces. The occurrence of Cl. perfringens in clinically healthy horses was not influenced by age. In animals fed exclusively on grass silage Cl. perfringens was found more frequently. The enterotoxin of Cl. perfringens was identified in one of 36 faecal samples from horses with diarrhoea. 54 Cl. perfringens strains isolated from material examined were studied for their ability to sporulate and to produce enterotoxin. 98% of the strains formed spores. Enterotoxin production was negative in all cases. Experimental investigations with enterotoxin producing Cl. perfringens strains on four ponies did not show any signs of a possible durable colonization of the intestinal tract by this organism. The results of these investigations do not suggest any pathogenic relevance of enterotoxin producing Cl. perfringens strains to the development of enteropathy in horses.

[The diagnosis of listeria encephalitis in ruminants using cultural and immunohistologic methods. II. Immunohistologic studies in formalin-fixed paraffin sections]. / Untersuchungen zur Diagnostik der Listerienenzephalitis bei Wiederkäuern unter Anwendung kultureller und immunhistologischer Verfahren. II. Mitteilung: Immunhistologische Unterschungen an formalinfixierten Paraffinschnitten.

The unlabelled peroxidase-antiperoxidase (PAP)-technique was compared with the avidin-biotin-peroxidase-complex (ABC)-technique in the identification of Listeria antigen in formalin-fixed paraffin sections of 58 ruminant brains, 44 of which showed histopathological lesions typical for listeric encephalitis. Rabbit hyperimmune serum, obtained by immunization with a Listeria monocytogenes strain of serotype 1/2 a, served as primary serum in the immunohistochemical investigations. The antiserum was tested for specificity using formalin-fixed smears of various bacterial species. Listeria antigen was demonstrated in 40 of the 44 brains with histopathological CNS alterations, as seen in listeriosis, using the ABC method, whereas their identification with the PAP method only succeeded in 34 of the brains. Despite the higher dilution of the primary antibody, the ABC method distinguished itself further, in comparison with the PAP method, through more intense immunohistochemical staining of Listeria antigen. Listeria spp, could be isolated from 46 of 52 brains, which were also examined bacteriologically. They could be isolated from 14 brains, which showed no histopathological lesions indicative of listeriosis. In contrast to this, Listeria antigen was only detected immunohistologically in brains with typical histological listeric CNS alterations. In three cases the presumptive histological diagnosis could not be confirmed immunohistologically.

[The diagnosis of Listeria encephalitis in ruminants using cultural and immunohistological techniques. I. Comparison of different selective media and culture techniques for the detection of Listeria from ruminant brains]. / Untersuchungen zur Diagnostik der Listerienenzephalitis bei Wiederkäuern unter Anwendung kultureller und immunhistologischer Verfahren. I. Mitteilung: Vergleich verschiedener Selektivnährmedien und Kulturverfahren beim Nachweis von Listerien aus Wiederkäuergehirnen.

The selective L-PALCAMY differential enrichment broth, the Listeria enrichment broth of the International Dairy Federation, Oxford Listeria selective agar, and PALCAM Listeria selective agar were comparatively examined in the cultural isolation of Listeria spp. from ten ruminant brains. The L-PALCAMY medium proved to be superior to the IDF broth in both selectivity and productivity for Listeria spp. in the brain samples, which were also contaminated with other bacteria. The Oxford and PALCAM agars corresponded in their productivity for Listeria spp. The latter, however, was more selective than the Oxford agar. Bacterial counts of up to 1.2 x 10(9) CFU/g of brain stem sample were made from Listeria monocytogenes (L.m.), and up to 6.2 x 10(4) CFU/g from Listeria innocua. A total of 164 brains from ruminants showing CNS disturbances and/or pathoanatomical CNS alterations were examined using L-PALCAMY medium, and Oxford and PALCAM agar. L.m. could be isolated from 29 of the brains, and Listeria innocua from five. Cultural isolation of both Listeria spp. occurred in one brain. Of 27 brains containing L.m., which were also examined using cold enrichment, L.m. was isolated in 59.3% of the cases with direct culture, in 81.5% of the cases using selective warm enrichment, and in 77.8% of the cases by means of selective cold enrichment. Five cases each were identified solely by cold or warm enrichment, respectively. In investigations of further 69 ruminant brains the number of brains shown to contain L.m. could be increased from seven to 13 by means of selective cold enrichment for three months.

[The effect of bacterial interference phenomena in experimental Staphylococcus hyicus infections of gnotobiotic piglets]. / Untersuchungen über die Wirkung bakterieller Interferenzphänomene bei der experimentellen Staphylococcus-hyicus-Infektion gnotobiotischer Ferkel.

The prevention of exudative epidermitis could be confirmed in experimental investigations with gnotobiotic piglets when the skin first was colonized with avirulent strains of Staphylococcus (Staph.) hyicus and subsequently exposed to virulent strains of Staph. hyicus. However, locally restricted cutaneous lesions in the area of application corresponding to exudative epidermitis were seen in five of nine piglets. Using the strain Staph. sciuri the spread of virulent Staph. hyicus could not be suppressed. Such infected two piglets developed generalized exudative epidermitis. In another experiment with four piglets it could be shown, that the relative protective mechanism correlating to bacterial interference on the one hand can be influenced by the virulence of causative organisms. On the other hand it even can be abolished when skin lesions are involved. For that reason probably the utilization of bacterial interference in prevention of exudative epidermitis under field conditions is considerably limited.

[Bacteriologic studies of the occurrence of Chlamydia psittaci in organs of swine and in aborted swine fetuses]. / Bakteriologische Untersuchungen zum Vorkommen von Chlamydia psittaci in Organen von Schweinen und in abortierten Schweinefeten.

By inoculation of embryonating chicken eggs via the yolk sac route Chlamydia psittaci was grown from 11 lungs of 45 pigs with pneumonia (24.4%). From the lungs of 55 pigs with other diseases the organism was isolated in five cases (9.1%). Chlamydiae were not detectable by cultural methods in the uterine mucosa of 87 sows, arthritic joints of 30 store pigs and in aborted fetuses. A commercial available enzyme amplified immunoassay indicated the presence of chlamydial antigen in mucosal scrapings from the uterus of two sows and in the fetal membranes as well as fetal organs in one case of porcine abortion.

[The occurrence of enterotoxigenic Clostridium perfringens strains in the feces of dogs and cats]. / Zum Vorkommen enterotoxinbildender Clostridium-perfringens-Stämme im Kot von Hunden und Katzen.

A total of 147 faecal specimen of dogs and cats was examined with cultural method for the occurrence of Cl. perfringens and its enterotoxin by using a reversed passive latex agglutination test (Pet-RPLA). Cl. perfringens could be detected in 77.9% of the samples of dogs (n = 68) and 65.6% of cats (n = 32) with diarrhoea in germ counts of 10(4)-10(10) cfu/g faeces. In the group of non diarrhoeic dogs (n = 39) and cats (n = 8) Cl. perfringens was found in 53.9% and 50% of the samples, the germ counts revealed 10(4)-10(8) cfu/g faeces. The enterotoxin of Cl. perfringens was detected in 48.5% of the samples of dogs and 28.1% of cats with diarrhoea. On the other hand this toxin could not be detected in any faecal specimen of non diarrhoeic animals. The in vitro detection of Cl. perfringens enterotoxin was successful at 65% of 20 strains, that had been isolated from faecal samples with enterotoxin. Also 2 Cl. perfringens strains isolated from faeces of non diarrhoeic dogs proved to be enterotoxigenic. The present test results given reason to believe that enterotoxigenic Cl. perfringens strains are involved in the complex of enteric disease of dogs and cats.