Results of the 2018 Rapid DNA Maturity Assessment.

Three commercially available integrated rapid DNA instruments were tested as a part of a rapid DNA maturity assessment in July of 2018. The assessment was conducted with sets of blinded single-source reference samples provided to participants for testing on the individual rapid platforms within their laboratories. The data were returned to the National Institute of Standards and Technology (NIST) for review and analysis. Both FBI-defined automated review (Rapid DNA Analysis) and manual review (Modified Rapid DNA Analysis) of the datasets were conducted to assess the success of genotyping the 20 Combined DNA Index System (CODIS) core STR loci and full profiles generated by the instruments. Genotype results from the multiple platforms, participating laboratories, and STR typing chemistries were combined into a single analysis. The Rapid DNA Analysis resulted in a success rate of 80% for full profiles (85% for the 20 CODIS core loci) with automated analysis. Modified Rapid DNA Analysis resulted in a success rate of 90% for both the CODIS 20 core loci and full profiles (all attempted loci per chemistry). An analysis of the peak height ratios demonstrated that 95% of all heterozygous alleles were above 59% heterozygote balance. For base-pair sizing precision, the precision was below the standard 0.5 bp deviation for both the ANDE 6C System and the RapidHIT 200.

Efecto del consumo agudo de cafeína sobre la fuerza máxima y los niveles de lactato en sangre en jóvenes sedentarios: Ensayo clínico aleatorizado / Effect of acute caffeine consumption on the maximum force and blood lactate levels in sedentary young: Randomized clinical trial

Objetivo: El propósito del estudio fue demostrar el efecto de la cafeína en consumo agudo sobre la fuerza máxima y los niveles de lactato. Material y métodos: Ensayo clínico aleatorizado controlado de grupos paralelos ciego simple (participante). Se seleccionaron 92 sujetos, 43 fueron asignados aleatoriamente al grupo de ingesta de cafeína (1,3 mg/Kg) o el control con placebo. Se utilizó el test de 1RM para medir la fuerza máxima y el lactato se determinó mediante espectrofotometría (Kit comercial Sigma) antes y después de 1 hora de la ingestión de cafeína (1,3 mg/Kg) o placebo. El análisis estadístico se realizó con la prueba t-student, el cambio porcentual y el tamaño del efecto con la d-Cohens, p ≤ 0,05. Resultados: Se produjo una mejoraría significativa en la fuerza máxima de miembro superior de 1RM 8,3 ± 6,0 kg con un tamaño efecto medio (26,2%; d= 0,64; t= -4,36; gl=41; p <0,01; IC: -16,9 a -6,2); y de miembro inferior de 1RM 9,2 ± 4,9 kg con un tamaño del efecto medio (25,8%; d= 0,51; t= -3,16; gl=41; p <0,01; IC: -17,0 a -3,7). Para los niveles de lactato se observó un aumento menor en el grupo experimental 0,96 ± 0,10 mmol/L con un tamaño del efecto pequeño (64,9%; d=0,39; t= 10,8; gl=41; p <0,01; IC: 0,55 a 0,81). Conclusiones: La cafeína en dosis baja produce un incremento superior en la fuerza máxima de miembro superior, seguido por la fuerza máxima de miembro inferior y favorece un incremento menor de los niveles de lactato.

Objective: The aim of this study was to demonstrate the effect of caffeine in acute consumption on the maximum force and lactate levels. Material and methods: A randomized controlled trial of single-blind parallel group (participant) was done where 92 subjects were selected, 43 were assigned randomly to the caffeine intake group (1, 3 mg/Kg) or placebo control. The 1RM test was used to measure the maximum force and the lactate was determined by spectrophotometry (Sigma Kit commercial) before and after an hour of caffeine intake (1, 3 mg/Kg) or placebo. The statistical analysis was performed using the student t- test, and the percentage change and the effect size with Cohen's d-p ≤ 0.05. Results: There was a significant improve in the maximum force of upper limb of 1RM 8.3 ± 6.0 kg with an average effect size (26.2%; d = 0.64, t = -4.36, df = 41, p <0.01; CI: -16.9 to -6.2); and lower limb 1RM 9.2 ± 4.9 kg with a medium effect size (25.8%; d = 0.51, t = -3.16, df = 41, p <0.01, CI : -17.0 to -3.7). An increase in the lactate levels was observed in the experimental group 0.96 ± 0.10 mmol/L with a small effect size (64.9%; d = 0, 39; t = 10.8; gl = 41; p < 0.01;) CI: 0.55 to 0.81). Conclusions: Caffeine in low doses produces a higher increase in the maximum strength of upper limb, followed by the maximum force of lower limb and favors a smaller increase in lactate levels.