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3.
Artigo em Inglês | MEDLINE | ID: mdl-8081540

RESUMO

Africanized honey bees and the wasp Polistes versicolor are common insects in Brazil; their venoms are composed of a complex mixture of components which present several biological activities. Stinging accidents are very frequent and are generally followed by important clinical reactions, and even deaths are not uncommon. In the present study, venom was extracted from Africanized honey bees and P. versicolor, and it was biochemically characterized and the antigenic cross-reactivity was investigated by Western blot analysis and specific IgE determination by ELISA in the sera of subjects allergic to each venom. The honey bee venom presented higher phospholipase A2 and hyaluronidase activities than P. versicolor venom, which in turn presented higher lipase, acid phosphatase and esterase activities. A high incidence of false-negatives was also observed during determinations of specific IgE for P. versicolor venom when the kits with venoms from wasps of temperate climates were used, suggesting that the diagnosis of allergy to neotropical wasp venom must take into consideration the clinical history and skin tests.


Assuntos
Venenos de Abelha/imunologia , Venenos de Vespas/imunologia , Adolescente , Adulto , Venenos de Abelha/análise , Criança , Reações Cruzadas , Feminino , Humanos , Imunoglobulina E/sangue , Masculino , Pessoa de Meia-Idade , Peso Molecular , Venenos de Vespas/análise
5.
J Chromatogr ; 368(1): 145-51, 1986 Oct 10.
Artigo em Inglês | MEDLINE | ID: mdl-3782362

RESUMO

The quantitative determination of triacylglycerols separated according to acyl carbon numbers and level of unsaturation was studied on two 10-m immobilised SE-54 columns. The fat samples were silylated before on-column injection. The 0.2 mm I.D. column showed higher resolving power and the 0.32 mm I.D. column better repeatability. The application to real quantitations was investigated with analyses of butter-fat solid and liquid fractions and of untreated and interesterified 70-30 mixtures of butter-fat solid fraction and hydrogenated rapeseed oil. Changes in the triacylglycerol composition induced by interesterification and fractionation are so pronounced that analysis on capillary columns can be recommended, in spite of only fair repeatability.


Assuntos
Triglicerídeos/análise , Animais , Brassica/análise , Manteiga/análise , Bovinos , Cromatografia Gasosa
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