Vitamin E ameliorates testicular histological features and androgen binding protein levels in testicle of rats induced by allethrin.

OBJECTIVE: This study aims to determine the ameliorative effect of vitamin E (vit E) on histological features and androgen binding protein (ABP) levels in rats induced by allethrin. MATERIALS AND METHODS: Thirty sexually mature male Wistar rats weighing between 200 and 300 gm, and aging 3 months were taken for this study and were divided into three groups: negative control (NC), positive control (PC), and treatment (T) groups. The PC and T groups were induced by allethrin 12 h per day for 31 days; however, only the T group was given vit E orally at 1 ml/gm body weight (BW) each day for 14 days. The paraffin block method was used to measure tubules' diameter, thickness of the seminiferous epithelial layer, and Sertoli cell number. The ABP levels were measured by enzyme-linked immunosorbent assay. RESULTS: The results showed that vit E gave significant effect (p < 0.05) on tubular diameter at NC 123.67 ± 12.77, PC 147.16 ± 10.64, and T 130.08 ± 10.00; tubular epithelial thickness at NC 33.55 ± 3.21, PC 30.02 ± 1.53, and T 32.96 ± 2.81; Sertoli cells number at NC 55.48 ± 5.9, PC 43.84 ± 3.77, and T 53.44 ± 4.26; and ABP levels at NC 72.35 ± 39.06, PC 38, 48 ± 18.78, and T 86.10 ± 35.77, respectively. CONCLUSION: This study concludes that vit E has an ameliorative effect against the toxic effects of allethrin at testicular histological features and ABP levels.

The Comparison of RhoC and PI3K Gene Expression on the Breast Cancer Tissue and Benign Tumour Tissue.

BACKGROUND: The expression of a gene is a process that conveys information of genes to synthesise gene product is functional. Alterations of the molecular biology in breast cancer are very complex because of many factors play a role in the tumorigenesis. RhoC is a prometastases gene. The PI3K gene is crucial in the regulation of multiple cellular functions, including cell growth, proliferation, metabolism and angiogenesis. AIM: This study aims to compare of RhoC and PI3K gene expression on the breast cancer tissue and benign tumour tissue. MATERIAL AND METHODS: Expression of the RhoC and PI3K genes was carried out with qPCR. The absolute quantification method was using breast cancer tissue. As a comparison, benign tumours (FATs) tissue was carried out. The standard curves were obtained from cloning target genes, which were inserted into the gGEMT-easy vector from E. coli. The gene expression data was carried out by t-test to see the mean difference between the expression of breast cancer tissue and benign tumours (FATs) with a value of p ≤ 0.005 in RhoC and PI3K gene expression. And the relationship between expressions was done by Pearson correlation test. RESULTS: The results showed that it was found that PI3K gene expression on breast cancer tissue was higher than the number in a benign tumour (fibroadenoma mammae) as an endogenous control. And also, the expression of RhoC is much lower on breast cancer tissue compared with a benign tumour. CONCLUSION: This study concluded that expression of RhoC affects the expression of PI3K so that the thing this is what causes the proliferation and began to provide support aggressive cancer cells in the breast.

Evaluation of MiR-21 and MiR-10b Expression of Human Breast Cancer in West Sumatera.

BACKGROUND: Abnormal expression of several microRNAs (miRNAs) has been demonstrated in many types of cancer tumor tissue. The miR-10b and miR-21 are an oncogenic miRNAs which play role in proliferation and invasion of Breast Cancer (BC) tumorigenesis. The aim of this study was to evaluate the miR-21 and miR-10b expression in BC in West Sumatran women, Indonesia. MATERIALS AND METHODS: A total of 40 samples, consisting of 30 samples of breast cancer tissues (BC) and 10 samples of fibroadenoma tissues (FATs) as control and non-cancerous were analyzed. The miR-21 and miR-10b expression of each sample were investigated by using realtime PCR, followed by universal Reverse Transcription (RT) then real-time PCR amplification with specific primers. Hsa-miR-16-5p LNA PCR primer was used as an endogenous control. RESULTS: The results showed that the expression level of miR-21 was more than 4 times higher in BC than in FATs. The expression level of miR-10b was lower in BC than FATs, by a factor of 3.34 fold. Both these differences were statistically different (p = 0.001). CONCLUSION: In this study it was concluded that for this sample of West Sumatran Women miR-21 expression in BC was higher than in FATs, whereas miR-10b was lower in BC than in FATs.