Effect of educational intervention on preventive behaviors of brucellosis among health volunteers in Rafsanjan city: Application of health belief model.

BACKGROUND: Human brucellosis can be a source of problems that affect public health, social, and economic well-being of the world's population. This study was conducted with the aim of determining the effect of Educational Intervention (EI) based on Health Belief Model (HBM) on preventive behaviors against brucellosis in Health Volunteers (HVs) in Rafsanjan. MATERIALS AND METHODS: Randomly, 104 HVs, in the intervention and control group, participated in a quasi-randomized, controlled experimental study. Variables were evaluated before and 1 month after intervention. In the intervention group, the educational program was conducted with lecture, group discussion, showing movies and related photos, booklets, and pamphlets. The program included five 45-min sessions that developed regarding the beliefs and constructs of HBM about brucellosis and its prevention methods. Data regarding HBM constructs and preventive behavior were collected using the questionnaire with 100 items by the self-report method. Finally, the data were entered into the SPSS software version 16.0, and statistical tests such as Chi-square, independent and paired t-test, Mann-Whitney, and Wilcoxon test were used for the data analysis at the significant level of 0.05. RESULTS: Prior to the intervention, the mean score of the HBM constructs and preventive behaviors between the two groups did not differ significantly, but 1 month later, in the intervention group increased significantly compared to the control group (P < 0.05). CONCLUSION: We recommend to health authorities and health-care providers to use HBM in EIs to create susceptibility, increase perceived severity and benefits, promote self-efficacy, uses cue to action, as well as reduce behavioral barriers, and ultimately adopt health-promoting behaviors.

Design, implementation, and evaluation of a PRECEDE-PROCEED model-based intervention for oral and dental health among primary school students of Rafsanjan city: a mixed method study.

BACKGROUND: Oral and dental health has a significant impact on public health as well as the quality of life among individuals and families. This study aims to design, implement, and evaluate an intervention based on the PRECEDE-PROCEED model for oral and dental health among primary school students in Rafsanjan city, Southern Iran. METHODS: According to the nature of the model and with the focus group discussion and interview methods, in phases 1 to 4 (PRECEDE), predisposing, enabling, and reinforcing factors related to oral health were identified. The training program was designed and consisted of six sessions for students (250 students) with a brushing session, three sessions for parents, and two sessions for teachers. Process evaluation and the effect of the program on behavioral and factors affecting oral health were evaluated (PROCEED). RESULTS: In the qualitative section, seven predisposing factors, five enabling factors, and two reinforcing factors were identified. A training program based on the PRECEDE-PROCEED model was found to be effective in increasing the mean scores of the above constructs and the students' brushing behavior (P < 0.001). CONCLUSION: Based on the model planning phases, the factors affecting the brushing behavior of children aged 6-12 years were identified. The educational program has had a significant effect on improving the scores of predisposing, enabling and reinforcing factors and children's brushing behavior.

The Effect of Integrated Intervention Based on Protection Motivation Theory and Implementation Intention to Promote Physical Activity and Physiological Indicators of Patients with Type 2 Diabetes.

Despite benefits of physical activity, the level of physical activity is not desirable in patients with type 2 diabetes. The aim of this study is the using of integration of intervention based on the theory of protection motivation and implementation intention in order to improve the level of activity in patients with diabetes. This field trial study has been performed on 125 patients with type 2 diabetes. Samples have been randomly selected, and they are divided into two intervention and control groups. In the intervention group, training sessions were conducted based on the protection motivation theory and implementation intention. Physical activity levels, VO2 max, and hemoglobin A1C were measured before and three months after the intervention in the two groups. Data were analyzed by using SPSS 18, and independent t-test, paired t-test, and equivalent nonparametric tests were used for analyzing abnormal data. The results of this study showed that the level of physical activity was higher in the intervention group (p = 0.02). Also, the amount of hemoglobin A1c in the intervention group has been decreased significantly three months later (p < 0.001). In this study, VO2 max and blood lipids were not significantly different in the two groups. However, there was higher VO2 max compared to before the intervention in the intervention group. The present study showed that combining motivational interventions and implementing intention intervention can be effective in promoting the physical activity of patients with type 2 diabetes.

Detection and characterization of the Isospora lunaris infection from different finch hosts in southern Iran.

This study was conducted to investigate the Isosporoid protozoan infections in finch types. Fecal samples were collected from marketed domestic Java sparrows (Lonchura oryzivora), colored and white Zebra finch (Taeniopygia guttata), and European goldfinch (Carduelis carduelis) in southern Iran. The coccidial oocysts were recovered and investigated according to the morphological features and the ribosomal gene markers. Additionally, a challenge infection was conducted with 5 × 104 and 5 × 103 sporulated oocysts in four java sparrows to estimate the clinical manifestations. Based on the morphology, the oocysts of Isospora lunaris were identified in all sampled bird types; however, the molecular method revealed the isolates had considerable similarities with some of Isospora and systemic Isospora-like organisms named as Atoxoplasma. Phylogenetic data also constructed an Atoxoplasma/Isospora clade with high sequence identities. High dose of the challenge with the parasite led to severe depression and sudden death, but it did not coincide with remarkable lesions and parasitic invasion in visceral organs. Contrary to molecular results, this feature is consistent with the common Isospora infections in passerines and differs from those described for Atoxoplasma species. Because of the prevalence, possibility of transmission, and clinical consequences, preventive measures are necessary to avoid outbreaks of isosporoid infections among finch type birds.

Molecular Characterization of Fasciola spp. from a Donkey (Equus asinus) Using Partial Sequencing of cox1 and nad1.

BACKGROUND: Fasciola hepatica as an important parasite affects health of humans and animals in some tropical and subtropical areas of the world, including Iran. Little is known about the molecular diversity of Fasciola in Equidae. Therefore, this study aimed to characterize the genetic polymorphisms among parasites. METHODS: Eight adult Fasciola spp. isolates were collected from a working donkey after necropsy in Shiraz, southwestern Iran, in 2018. Primarily, various parameters were measured morphologically. Subsequently, DNA was extracted from each fluke and molecular markers of cytochrome C oxidase (cox1) and NADH dehydrogenase 1(nad1) from individual Fasciola isolates were amplified using PCR assay and sequence data were employed for molecular and phylogenetic analysis. Genetic diversity between isolates was evaluated by comparing the sequences of these two mitochondrial regions. RESULTS: Based on the morphological and analyzed mitochondrial sequences, all of eight donkey isolates (100%) were identified as F. hepatica. Moreover, nine and five nucleotide polymorphisms were identified in the cox1and nad1 region sequences, respectively. CONCLUSION: Accordingly, phylogenetic data revealed five and four haplotypes among donkey isolates based on the cox1and nad1 markers. Similarly, some of these haplotypes have been previously reported from different host species in Iran as well as all around the world.

Determiners of fast-food consumption in Iranian university students: Application of prototype/willingness model.

BACKGROUND: Today, marked by advanced scientific approach, urbanism, and changing life style, there is an ever-growing rate of fast-food consumption, which has significantly affected public health. Hence, the present research aimed to predict factors involved in fast-food consumption in light of the prototype/willingness model among the students of Rafsanjan University of Medical Sciences. MATERIALS AND METHODS: In the present descriptive/analytical research conducted in 2018-2019, 350 students in Rafsanjan University of Medical Sciences were selected through proportionate stratified random sampling. The required data were collected using a self-administrated questionnaire in light of the constructs within the prototype/willingness model and fast-food consumption behavior. The data were analyzed in SPSS version 16.0 using descriptive and inferential statistics including mean scores, standard deviation, correlation coefficient, and linear regression. RESULTS: Behavioral intention of fast-food consumption showed to be positively correlated with positive willingness, positive prototype, positive subjective norms, and positive attitude toward fast-food consumption. Furthermore, the correlation between positive behavioral intention of consuming fast food and positive subjective norms and positive attitude toward fast-food consumption was statistically significant (P < 0.05). The underlying constructs of prototype/willingness model altogether managed to explain 13% of the total variance of fast-food consumption behavior. Behavioral intention showed to be the strongest predictor of the behavior (ß = 1.613). CONCLUSION: It is suggested that the preventive measures aimed to reduce fast-food consumption among the youth focus more on strengthening negative attitudes and subjective norms concerning fast-food consumption.

Effect of education based on health belief model on observation of standard precautions by dental students in Rafsanjan in 2019.

INTRODUCTION: It is vital importance to observe standard precautions (SPs) in dentistry to prevent the transfer and spread of blood-borne diseases in the community. The aim of the present study was to evaluate the effect of educational intervention using health belief model (HBM) on the observation of SPs by dental students in 2019. MATERIALS AND METHODS: Eighty-seven dental preclinical students were included in the present quasi-experimental study. The students were randomly assigned to two groups, and data were collected using a questionnaire designed based on HBM. Educational intervention was carried out after the pretest analysis in four 60-min sessions. Data were analyzed with independent t-test and paired t-test using SPSS 16 at a significance level of P < 0.05. RESULTS: After the educational intervention, the mean score of awareness (P = 0.001), perceived susceptibility (P = 0.01), perceived severity (P = 0.02), perceived benefits (P < 0.001), cues to action (P = 0.006), self-efficacy (P = 0.002), and behavior (0.03) in the intervention group was significantly increased. CONCLUSION: Despite the effect of education on increasing the students' scores in HBM constructs and observation of SPs, the most important barriers to the adoption of precautionary behaviors were deficiencies in protective tools and aids.

Phylogenetic analysis of goat warble fly (Przhevalskiana silenus) based on mitochondrial COI gene.

The larvae of the genus Przhevalskiana (Diptera: Oestridae) are the causative agents of subcutaneous myiasis in goats. Several species have been grouped under this genus based on the morphology of different larval stages, albeit with a lot of uncertainties. Thus, application of genetic tools seems to be helpful for taxonomy. During this study, the cytochrome oxidase I (COI) gene was targeted for the characterization of larval stages of goat warble fly. Fragments of 606 bp were amplified for all the specimens. Based on the COI gene analysis, all the recovered specimens were identified as larvae of Przhevalskiana silenus. Molecular data on the genus is relatively rare but present isolates revealed about 87-89% identity with previous isolates of P. silenus. According to the phylogenetic data, the present isolates branched (as a sister group) with a number of Hypoderma spp. including H. bovis, H. diana, H. lineatum and H. sinense. The present findings confirmed that the COI gene could be a suitable marker for genetic characterization and identification of larvae up to the species level.

A Laboratory Strain of Leishmania major: Protective Effects on Experimental Leishmaniasis.

PURPOSE: Leishmaniasis, as one of the most important vector-borne and zoonotic diseases, can be seen in different forms and is more prevalent in developing countries worldwide. Due to the absence of effective strategies in its prevention, treatment, and control, investigation of effective control strategies against the disease is necessary. In this research, we evaluated the immunogenicity of a cold-adapted laboratory strain of Leishmania major (LMC) in the mouse model. METHODS: Twenty BALB/c mice were divided into two groups. LMC group received 4 × 106 of LMC strain in 0.5 ml DMEM, and VLM group, as the control group, received 0.5 ml Dulbecco's modified Eagle's medium. Both groups were challenged with virulent L. major 3 weeks after inoculation. RESULTS: The data obtained from the analysis of immune responses and histopathological changes interestingly revealed protection against L. major in immunized mice. Compared with the VLM group, the mice immunized with LMC strain of L. major in the LMC group showed a significant increase in IFN-γ and IgG2a levels (P < 0.05) which are important indexes for Th1-related immune responses. Additionally, significant differences in concentration of IgG1 and IgG total before and after the challenge was observed in LMC group (P < 0.05). Furthermore, the immunized mice showed a significant reduction in mean sizes of skin lesion and liver damage compared to the VLM group. CONCLUSION: Based on the present findings on immunogenicity of LMC strain, it seems this strain is able to induce both humoral and cellular immunity and a significant protection against L. major in the mouse model.

Study On Social Support for Exercise And Its Impact on the Level of Physical Activity of Patients with Type 2 Diabetes.

BACKGROUND: Physical activity is one of the most important self-care approaches to controlling complications of type 2 diabetes. According to Bandura's social theory, factors such as social support are effective factors in the incidence of the behaviour. AIM: This study aims to determine the level of physical activity, social support and their determinants. METHODS: This descriptive study was performed on 250 patients with type 2 diabetes by Cluster-Random Sampling method in Rafsanjan City. Data were collected using the International Physical Activity Questionnaire and Social Support Questionnaire for Sport, that their validity and reliability were confirmed. The results were analysed by t-test, ANOVA and logistic regression. RESULTS: This study showed that 46.8% of the patients were in the inactive group. Social support score for exercise was low in this group. The results indicated that social support and gender are predictors of physical activity, and with an increase in the social support score, the odds of having minimal physical activity increased 1.17 fold (OR = 1.167) and men were 4.18 times more likely to have minimal physical activity (OR = 4.183). CONCLUSION: Considering the low level of physical activity and social support in diabetic patients, and the effect of social support on the prediction of physical activity, interventions are recommended to increase social support in this group.

Prevalence and molecular characterization of Cryptosporidium spp. and Giardia intestinalis in household dogs and cats from Shiraz, Southwestern Iran.

In the present study, a total of 615 fecal samples from veterinary clinics were screened by microscopy for the presence of Cryptosporidium and Giardia oocysts. Molecular genotyping of Cryptosporidium and Giardia were carried out using PCR and sequence analysis. Overall, Cryptosporidium and Giardia oocysts were detected in the 0.6% (2/315) and 1.9% (6/315) of dogs and in the 0.7% (2/300) and 1.3% (4/300) of cats, respectively. Sequencing revealed the presence of C. canis (n = 2) in dogs and C. felis (n = 2) in cats. Moreover, G. intestinalisassemblage D (n = 2), C (n = 3) and A, sub-assemblage AII (n = 1) were identified in dogs; G. intestinalis assemblage F (n = 3) and assemblage A, sub-assemblage AI (n = 1) were identified in cats. The highest prevalence of Giardia was observed in dogs younger than one year (6/315), and in those with diarrhea (p < 0.05). Data of the study suggest that dogs and cats play a minor role in the zoonotic transmission of cryptosporidiosis and giardiasis in Southwestern Iran.

Curcumin: A promising treatment for Cryptosporidium parvum infection in immunosuppressed BALB/c mice.

Members of the genus Cryptosporidium are frequent protozoan pathogens in humans and a wide range of animals. There is no consistently effective treatment against cryptosporidiosis, especially in immunodeficient patients. The present study was carried out to study the therapeutic effects of curcumin against cryptosporidiosis in immunosuppressed BALB/c mice. Mice were divided into five groups and immunosuppressed by dexamethasone. Three groups were inoculated with C. parvum oocysts, administered with curcumin, paromomycin, and without treatment. The reminders were regarded as controls. The oocysts in the fecal smear were counted daily. At days 0, 3, 7, and 11 post-treatment, the mice were sacrificed, and the efficacy of drugs was evaluated by comparing the histopathological alterations in jejunum and ileum, measuring the total antioxidant capacity, and malondialdehyde in the affected tissues. The infection was completely eliminated in the curcumin-treated group, and oocyst shedding stopped with no recurrence after drug withdrawal. On the contrary, paromomycin was unable to eliminate C. parvum infection completely, and oocyst shedding continued even 10 days after the drug withdrawal. Based on these findings, curcumin can be a trustworthy compound for the elimination of infection in immunosuppressed hosts. Further evaluation to find its accurate mechanism of action should be considered.

Influence of hydatid disease on the pregnancy outcomes: An experimental study.

AIM: Hydatid disease during pregnancy may lead to various complications in both mother and child. Accordingly, enough knowledge is required to manage these complications. This study was done to evaluate the interaction between hydatid disease and pregnancy in laboratory mice. METHODS: Twenty female BALB/c mice (Mus musculus) were divided into test and control groups, 10 animal in each. The mice of the test group were infected intraperitoneally by injection of 1000 protoescolices. Six months after infection, the mice of test and control groups were mated with male mice for a week. After parturition, the size and weight of babies were measured and compared between two groups. RESULTS: While all the mice of control group delivered healthy babies, 3 out of 10 mice of test group showed no pregnancy. The infected mice delivered lower number of babies than the mice of control group. The mean size of fetuses or babies obtained from the infected mice were statistically lower than those obtained from the mice of control group (P = 0.000). The weight of babies delivered by the infected mice not only at the time of birth (P = 0.005) but also 10 days after birth (P = 0.021), were significantly lower than those delivered by the mice of control group. The level of progesterone and estradiol in the mice of test group were significantly lower (P = 0.009) and higher (P = 0.043), respectively, in comparison to the mice of control group. CONCLUSION: Hydatid disease during pregnancy may considerably affect the pregnancy outcomes.

The Predictive Effects of Protection Motivation Theory on Intention and Behaviour of Physical Activity in Patients with Type 2 Diabetes.

INTRODUCTION: Theory-based education tailored to target behaviour and group can be effective in promoting physical activity. AIM: The purpose of this study was to examine the predictive power of Protection Motivation Theory on intent and behaviour of Physical Activity in Patients with Type 2 Diabetes. METHODS: This descriptive study was conducted on 250 patients in Rafsanjan, Iran. To examine the scores of protection motivation theory structures, a researcher-made questionnaire was used. Its validity and reliability were confirmed. The level of physical activity was also measured by the International Short - form Physical Activity Inventory. Its validity and reliability were also approved. Data were analysed by statistical tests including correlation coefficient, chi-square, logistic regression and linear regression. RESULTS: The results revealed that there was a significant correlation between all the protection motivation theory constructs and the intention to do physical activity. The results showed that the Theory structures were able to predict 60% of the variance of physical activity intention. The results of logistic regression demonstrated that increase in the score of physical activity intent and self - efficacy increased the chance of higher level of physical activity by 3.4 and 1.5 times, respectively OR = (3.39, 1.54). CONCLUSION: Considering the ability of protection motivation theory structures to explain the physical activity behaviour, interventional designs are suggested based on the structures of this theory, especially to improve self -efficacy as the most powerful factor in predicting physical activity intention and behaviour.

The field efficacy of Nigella sativa and Berberis vulgaris methanolic extracts against Haemoproteus columbae.

OBJECTIVE: The methanolic extracts of Nigella sativaL. seeds (MENS) and Berberis vulgaris L.(MEBV) were investigated for treatment of Haemoproteus columbae-infected pigeons (Columba livia domestica). MATERIALS AND METHODS: One hundred twenty naturally-infected pigeons were randomly divided into four groups of thirty each. Two groups were treated separately with the extracts, while the positive and negative control groups were given buparvaquone (Butalex®) and distilled water, respectively. The parasitaemia rate was calculated in all groups before and after the experiment at four-day intervals for16 days. RESULTS: The results showed a high therapeutic effect for MENS with a progressive decrease in average parasitaemia rate from 18.17% before treatment to 0.73% at the end of treatment (p<0.05), while Butalex® was able to suppress the parasitemia rate from 18.90% before treatment to 0.23% at the end of experiment (p<0.05). However, no significant changes in parasitemia rate were evident in groups treated with MEBV (p>0.05). CONCLUSION: Methanolic extracts of N. sativa showed therapeutic effects against H. columbae and may be regarded as a suitable choice for further studies to develop new drugs against blood parasites, in both animals and human beings.

Comparative efficacy of curcumin and paromomycin against Cryptosporidium parvum infection in a BALB/c model.

Cryptosporidium is a ubiquitous protozoan parasite causing gastrointestinal disorder in various hosts worldwide. The disease is self-limiting in the immunocompetent but life-threatening in immunodeficient individuals. Investigations to find an effective drug for the complete elimination of the Cryptosporidium infection are ongoing and urgently needed. The current study was undertaken to examine the anti-cryptosporidial efficacy of curcumin in experimentally infected mice compared with that of paromomycin. Oocysts were isolated from a pre-weaned dairy calf and identified as Cryptosporidium parvum using a nested- polymerase chain reaction (PCR) on Small subunit ribosomal ribonucleic acid (SSU rRNA) gene and sequencing analysis. One hundred and ten female BALB/c mice were divided into five groups. Group 1 was infected and treated with curcumin; Group 2 infected and treated with paromomycin; Group 3 infected without treatment; Group 4 included uninfected mice treated with curcumin, and Group 5 included uninfected mice treated with distilled water for 11 successive days, starting on the first day of oocyst shedding. The oocyst shedding was recorded daily. At days 0, 3, 7, and 11 of post treatments, five mice from each group were killed humanly; jejunum and ileum tissue samples were processed for histopathological evaluation and counting of oocyst on villi, simultaneously. Furthermore, total antioxidant capacity (TAC) and malondialdehyde (MDA) concentrations in affected tissues were also measured in different groups. By treatments, tissue lesions and the number of oocyst on villi of both jejunum and ileum were decreased with a time-dependent manner. In comparison with Group 3, oocyst shedding was stopped at the end of treatment period in both groups 1 and 2 without recurrence at 10days after drug withdrawal. Also, TAC was increased and the MDA concentrations were decreased in Group 1. Moreover, paromomycin showed acceptable treatment outcomes during experiment and its anti-cryptosporidial activity was faster than curcumin. The results confirmed the anti-cryptosporidial and antioxidant activity of curcumin against C. parvum and further evaluation of immunosuppressed animal models needs to be carried out.

Baseline Susceptibility of Filarial Vector Culex quinquefasciatus (Diptera: Culicidae) to Five Insecticides with Different Modes of Action in Southeast of Iran.

BACKGROUND: Culex quinquefasciatus (Diptera: Culicidae) is an important vector for many human diseases. The aim of this study was to evaluate the susceptibility level of larval and adult stages of Cu. quinquefasciatus to different groups of WHO recommended insecticides for vector control. METHODS: Larval stages of the Culex mosquitoes were collected from their natural habitats in Rafsanjan County at Kerman Province, southeast of Iran in 2016. Insecticide susceptibility status of adult female Cx. quinquefasciatus against DDT (4%), deltamethrin (0.05%), malathion 5%, and bendiocarb (0.1%) were determined using WHO standard insecticide susceptibility test. Additional test was carried out to determine the susceptibility status of larvae of Cx. quinquefasciatus to temephos. Bioassay data were analyzed by Probit program. RESULTS: Cx. quinquefasciatus adults showed resistance to all four groups of the tested insecticides according to the WHO criteria for resistance evaluation. The lethal concentrations for 50% mortality (LC50) and 90% mortality (LC90) of temephos against Cx. quinquefasciatus larvae were 0.18mg/l and 0.78mg/l, respectively. This finding also confirms resistance to temephos based on the WHO recommended instructions for resistance evaluation. CONCLUSION: Resistance to all groups of the tested insecticides should be considered for future vector control investigations in the study area.

The Attitude of Medical Students Toward Death: A Cross-Sectional Study in Rafsanjan.

AIM: Attitude toward death is one of the most important factors that can influence the behavior related to the health profession. It is thought that physicians are afraid of death more than other groups of specialist. Therefore, this study aimed to evaluate the attitudes of the medical students of Rafsanjan University of Medical Sciences toward death. MATERIALS AND METHODS: This study is a cross-sectional study on 308 medical students of Rafsanjan University of Medical Sciences in the academic year of 2015. Attitudes were assessed through the questionnaire of death attitude profile-revised. The collected data were analyzed upon arrival to a computer with SPSS version 14, and descriptive and inferential statistical methods. RESULTS: Attitude toward death was investigated in the 5 dimensions including the fear of death, death avoidance, approach acceptance, neutral acceptance, and escape acceptance. The results showed that the mean and standard deviations of fear of death, death avoidance, natural acceptance, approach acceptance, and escape acceptance were 3.76 ± 1.15, 3.54 ± 1.33, 5.14 ± 0.86, 4.66 ± 0.95, and 3.73 ± 1.25, respectively. It was found that people who have had the experience in dealing with death had less escape of the death attitude. CONCLUSION: Totally, the results of this study demonstrated that the medical students had good attitudes through 5 dimensions of attitudes toward death. This is probably due to the religious beliefs and also dealing with dying patients. However, it is recommended that training programs should be provided for students in the field of attitudes toward death.

The field efficacy of Lepidium latifolium and Zataria multiflora methanolic extracts against Varroa destructor.

Varroa destructor is the most serious pest of honeybee (Apis mellifera), causing high economic losses in the beekeeping industry worldwide. The intensive utilization of many chemical substances against the mites resulted in resistance development. One of the applicable and alternative treatments being used for their control is plant-derived products (PDSS). The aim of this study was to evaluate the acaricidal activity of Lepidium latifolium and Zataria multiflora leaf extracts on V. destructor in field conditions. Four different concentrations (100, 200, 400, and 500 ppm) of the methanolic extracts were sprayed to treat each colony. The efficacy and side effects on adult bees were compared to Apistan chemical strips (ACSS). The acaricidal activity was the highest (100%) for L. latifolium extract at 500 ppm after 12 days and 86.26% for Z. multiflora. The infestation rate was decreased to 0.0% with L. latifolium and to 13.74% with Z. multiflora. The highest reduction was observed with L. latifolium followed by Z. multiflora extract at 500 ppm concentration. Both of the extracts showed negligible effect on bees, and it can be concluded that these PDSS as biodegradable agents could be used for V. destructor control in honeybee colonies.

Study on small ruminant lungworms and associated risk factors in northeastern Iran.

OBJECTIVE: To determine the prevalence, identify the species involved and assess possible risk factors of lungworm infection in small ruminant slaughtered in abattoir of Mashhad in the northeast of Iran from October 2010-August 2011. METHODS: Faecal and post mortem examination were conducted on 350 and 2 500 animals, respectively. RESULTS: The overall prevalence of lungworm infection was 10.85% and 3.80% in coproscopic and post mortem examination respectively, and this difference was found to be significant. Higher prevalence of lungworm infection was recorded in post mortem examination in sheep (4.1%) than in goats (0.5%) (P< 0.05). The proportion of infection with Dictyocaulus filaria, Protostrongylus rufescens and mixed infection were 3.7%, 0.1% and 0.2% in sheep while in goats, the infection was reported with Dictyocaulus filaria (0.5%) only. The seasonal dynamics of lungworm infection showed that prevalence was highest in winter (7.8%) with a remarkable decline during the dry time (summer) (0.8%) which the difference was significant (P<0.001). The animals of less than one year old showed greater infection in post mortem examination than older animals significantly (P<0.001). Also, the infection rate between male and female animals showed significant difference (P<0.05) with prevalence rate of 4.6% and 2.0%, respectively. CONCLUSIONS: Due to its impact on production, emphasis should be given for the control and prevention of lungworm infection in this area.