Anthropozoonotic significance, risk factors and spatial distribution of Giardia spp. infections in quenda (Isoodon obesulus) in the greater Perth region, Western Australia.

Giardia spp. infections in wildlife populations have been linked to anthropogenic sources of infection and public health risk in a diversity of wildlife species and ecological locations worldwide. Quenda (Isoodon obesulus) remain in many urbanised areas of Perth, Western Australia, and can be gregarious in their interactions with humans and domestic animals. In a previous study, a high prevalence of Giardia spp. infection was identified amongst quenda trapped in urbanised environments and bushland in Perth, Western Australia. This study aimed to expand on that finding, by: identifying and estimating the prevalence of particular species of Giardia infecting quenda, and thus clarifying their anthropozoonotic/public health significance; identifying risk factors for Giardia spp. infection; and investigating putative associations between infection and indicators of ill health. Giardia spp. infections in Perth quenda are overwhelmingly of the host-adapted, non-zoonotic Giardia peramelis (apparent prevalence 22.2%; 95% CI 17.7-27.4%), indicating that quenda are not a substantial veterinary public health risk regarding this parasite genus. However, one case each of Giardia duodenalis and Giardia canis genotype D were identified in quenda trapped in urbanised environments (apparent prevalences 0.4%; 95% CI 0.1-1.9%). In quenda, Giardia spp. infection is associated with Cryptosporidium infection and flea infection intensity, which may reflect host population density, or regarding Cryptosporidium spp., similar transmission pathways or synergistic interactions between these taxa within the host. Giardia spp. infection is not associated with the measured indicators of ill health in Perth quenda, but this finding is representative of Giardia peramelis only, given the apparent rarity of other Giardia sp. infections in this study.

Validation of various parasite detection tests for use in the Australian marsupials quenda ( Isoodon obesulus) and brushtail possums ( Trichosurus vulpecula).

We aimed to validate the use of 1) the modified agglutination test and a polymerase chain reaction (PCR) protocol in detecting Toxoplasma gondii infection in quenda ( Isoodon obesulus) and brushtail possums ( Trichosurus vulpecula); 2) immunofluorescence microscopy of feces and a PCR and sequencing protocol in detecting Giardia spp. infection in quenda; and 3) a fecal flotation protocol in detecting gastrointestinal helminth infections of quenda. Quenda and brushtail possum carcasses, and samples from trapped quenda, were tested with 2 parasite detection tests per parasite, and results were modeled using Bayesian latent class analysis to estimate test sensitivity and specificity. The modified agglutination test and the PCR protocol were highly specific at detecting T. gondii infections in quenda and brushtail possums (≥93%); however, data were insufficient to assess sensitivity with adequate precision. Immunofluorescence microscopy and the PCR and sequencing protocol were both highly specific at detecting Giardia spp. in quenda (≥96%), but the PCR and sequencing protocol was relatively insensitive (69%, 95% credible interval [CrI]: 60-77%) compared to the highly sensitive immunofluorescence microscopy (98%, 95% CrI: 93-99%). The fecal flotation protocol was generally highly specific in the detection of gastrointestinal helminth infections (≥94%, with the exception of Trichuris spp. (88%, 95% CrI: 71-99%). The fecal flotation protocol was moderately to highly sensitive (≥74%) in the detection of strongyles, Labiobulura spp., Linstowinema spp., and Trichuris spp. Sensitivity was poor for detection of the cestode genus Potorolepis (36%, 95% CrI: 14-67%).