Comparación Placas de Titanio vs. Placas Reabsorbibles en Fracturas de Orbita: Revisión de literatura / Comparison of Titanium Plates vs. Resorbable Plates in Orbital Fractures: Literature Review

RESUMEN Introducción: Los tratamientos para fracturas de órbita se basan en la corrección del defecto de las lesiones orbitarias de tipo blow in y blow out (o de estallido), mediante diversas placas y mallas biocompatibles con el organismo, dándose una cicatrización de primera generación evitando un callo óseo y una fijación más rígida. Para el diagnóstico de este tipo de lesiones tenemos inflamación periorbitaria, enoftalmos, diplopía, equimosis, hemorragia subconjuntival. Existen diversos materiales reconstructivos siendo estos compuestos por distintas materias primas, como son los aloplásticos y autógenos; donde encontramos varios tipos como placas de titanio y las placas reabsorbibles siendo estas las más comunes y usadas actualmente, por su bajo estímulo a reabsorciones óseas y evitando efectos secundarios a largo plazo. Estas placas presentan diversos grados de ductilidad y resistencia. Se informó sobre varias complicaciones según el tipo de placas como es la cicatrización, las cirugías postquirúrgicas en caso de placas de titanio, etc. El objetivo de esta revisión es la evaluación de la eficacia las placas reabsorbibles versus placas de titanio en fracturas de órbita. Materiales y métodos: La investigación es de carácter documental, descriptivo y no experimental. En el cual se emplea una metodología de identificación e inclusión de artículos científicos tipo prisma. Resultados y conclusiones: Se verificaron las ventajas y desventajas tanto de las placas reabsorbibles como las de titanio siendo estas similares en la biocompatibilidad con el organismo humano, así como también varias diferencias como el soporte, fuerzas, resistencia de estas, concluyendo que es debatible el material ideal para tratar fracturas de órbita. Se seleccionaron artículos tomando en cuenta el título y objetivos; considerando estudios comparativos, revisiones sistemáticas, revisiones de literatura, los cuales comprendían criterios con respecto a fracturas de órbita y tratamientos quirúrgicos. La búsqueda arrojó 55 artículos en PubMed, 65 en Google, 4 en Scielo y 29 en Science direct, de los cuales se excluyeron libros, monografías, estudios experimentales, dando como resultado 21 artículos para el desarrollo de esta revisión bibliográfica. Y que fueron leídos y analizados en su totalidad, estudiando los objetivos, metodología y conclusión de cada uno de ellos para la posterior comparación.

ABSTRACT Introduction: Treatments for orbit fractures are based on the correction of the defect of blow in and blow out orbital injuries, by means of various plates and meshes biocompatible with the organism, giving a first-generation healing avoiding a bony callus and a more rigid fixation. For the diagnosis of this type of lesions we have periorbital inflammation, enophthalmos, diplopia, ecchymosis, subconjunctival hemorrhage. There are several reconstructive materials being these composed of different raw materials, such as alloplastic and autogenous, where we find several types such as titanium plates and resorbable plates being these the most common and currently used, for its low stimulus to bone resorption and avoiding long-term side effects. These plates have different degrees of ductility and resistance. Several complications have been reported depending on the type of plates, such as scarring, post-surgical surgeries in the case of titanium plates, etc. The objective of this review is to evaluate the efficacy of resorbable versus titanium plates in orbital fractures. Materials and methods: This research are a documentary, descriptive and non-experimental nature. A prism-type methodology of identification and inclusion of scientific articles was used. Results and conclusions: The advantages and disadvantages of both resorbable and titanium plates were verified, being these similar in biocompatibility with the human organism, as well as several differences such as support, forces, resistance of the same, concluding that it is debatable. The ideal material to treat orbital fractures. Articles were selected considering the title and objectives; considering comparative studies, systematic reviews, literature reviews, which included criteria regarding orbital fractures and surgical treatments. The search yielded 55 articles in PubMed, 65 in Google, 4 in Scielo and 29 in Science direct, from which books, monographs, experimental studies were excluded, resulting in 21 articles for the development of this bibliographic review. The 21 articles were read and analyzed in their entirety, studying the objectives, methodology and conclusion of each one of them for subsequent comparison.

Uso de elementos de protección personal en Unidad de Emergencia HBLT en contexto pandemia COVID-19 / Use of personal protection elements in the HBLT Emergency Unit in thecontext of the COVID-19 pandemic

Los elementos de protección personal (EPP) son el conjunto de equipamiento, componente de las precauciones estándares, destinados a proteger la piel y mucosas del operador evitando el contacto con agentes infecciosos. Los funcionarios de salud que tratan con pacientes COVID-19 se encuentran más expuestos a infectarse en comparación con el resto de los funcionarios de salud, razón por la cual deben utilizar de manera adecuada estos elementos de protección, para protegerse de una posible infección por COVID-19. El objetivo del estudio es deter-minar disponibilidad, manejo y uso EPP utilizados por los funcionarios del Servicio de Urgencia del Complejo Asistencial Barros Luco Trudeau. Este es un estudio transversal descriptivo realizado por medio de encuesta a los funcionarios de la Unidad de Emergencia HBLT a través de Google Forms®. Del total de funcionarios de la Unidad de Emergencia del Hospital Barros Luco Trudeau, un total de 123 funcionarios realizaron la encuesta cumpliendo con los criterios de inclusión, de los cuales 78 fueron mujeres y 45 hombres, con edades dentro del rango desde los 20 años a mayores de 61 años. Se observó que 39,0% de los funcionarios fue notificado como COVID-19 positivo. Un 60,2% de los funciona-rios tuvo una secuencia predeterminada para instalación de los EPP y un 68,3% para el retiro de los EPP. Los más utilizados fueron mascarillas quirúrgicas (95,9%), mascarilla KN 95 (88,6%) y guantes (90,2%). Este estudio contribuye a destacar la importancia en la correcta utilización de los EPP por parte de los funcionarios de la salud, para limitar los contagios y mejorar el cuidado de los equipos de salud durante la pandemia de COVID-19. Además, se describir un patrón de sintomatología más frecuente en los profesionales sanitarios del servicio de urgencia.

Personal protective equipment (PPE) is a set of equipment, a component of the standard precautions, intended to protect the skin and mucous membranes of the operator avoiding contact with infectious agents. Healthcare workers who deal with COVID-19 patients are at higher risk of being infected compared to other health professionals. Thus they must use them appropriately to protect themselves from possible COVID-19 infection. This study aimed to determine the presence, handling, and use of PPE by the personnel of the Emergency Service of the Barros Luco Trudeau Department. This descriptive cross-sectional study was carried out applying a survey to the staff of the HBLT Emergency Unit through the Google Forms® platform. A total of 123 staff of the Emergency Unit of the Barros Luco Trudeau Hospital that answered the survey met the inclusion criteria. Seventy-eight of them were women and 45 men, within the range of 20 to over 61 years old. Thirty-nine per cent of the personnel were notified as COVID-19 positive. Regarding PPE use, 60,2% of the personnel had a predetermined sequence for installation, and 68.3% had a correct sequence for removal of the PPE. The most used were surgical masks (95.9%), KN 95 masks (88.6%) and gloves (90.2%). Of those COVID-19 positive 46.3% were asymptomatic, and of those who had symptoms the most prevalent ones were headache (41.5%), tiredness (34.1%) and odynophagia (31.7%). This study highlights the im-portance of using the personal protective elements by health staff as self-care during the COVID-19 pandemic, in addition to describing the most frequent pattern of symptoms in health professionals in the emergency department.

Características del Trauma Maxilofacial de Tercio Medio Atendido en Unidad de Emergencia del Complejo Asistencial Barros Luco / Characteristics of Middle Face Trauma treated in an Emergency Unit of the Barros Luco Healthcare Unit

El Trauma Maxilofacial (TMF) es una patología que presenta alta frecuencia en la actualidad, constituyendo un problema global de salud para los centros de alta complejidad y los equipos multidisciplinarios involucrados en su manejo y resolución. (neurología, maxilofacial, otorrinolaringología, oftalmología). Su etiología y epidemiología varía significativamente en la literatura. El objetivo de este estudio es caracterizar a los pacientes atendidos en un hospital del s ector sur de Santiago, Chile, identificando sus factores sociales y clínicos, clasificando los principales tipos de fractura a nivel de tercio medio facial. Se realizó un estudio observacional retrospectivo con los Datos de Atención de Urgencia (DAU) de los pacientes atendidos en el Servicio Dental de la Unidad de Emergencia del Complejo Asistencial Barros Luco (CABL), analizando el sexo, edad, comuna de procedencia, etiología del trauma, categorización según el "Emergency Severity Index", horario de ingreso y evaluación, estructuras óseas comprometidas de los pacientes con diagnóstico final de Fractura en Tercio Medio Facial. Se utilizaron estimadores puntuales de porcentaje, media y desviación estándar para representar las variables. Se calculó la significancia de relación de variables independientes, con valor de p < 0,05 estadísticamente significativo por el tamaño muestral. Población principalmente afectada corresponden a hombres versus mujeres (2.4:1), y entre segunda y tercera década de vida. Principal etiología identificada fue accidentes en el hogar (n = 73), seguidos de agresiones (n = 55). La mayoría de los pacientes ingresan con un riesgo C4 (n = 107) y C3 (n = 64). Fractura más frecuentemente es la nasal (46 %), seguidas de complejo orbitario (11.3 %) y arco cigomático (7.9 %). Es necesario mantener actualizada la epidemiología del TMF considerando la complejidad de su manejo temprano, morbilidades al mediano y largo plazo, con el objetivo de tener un rol activo en su prevención y tratamiento.

Maxillofacial Trauma (TMF) is a frequent pathology nowadays and presents a global health problem for specialized trauma centers and the multidisciplinary teams involved in its management and treatment (neurology, maxillofacial, otolaryngology, ophthalmology). The objective of this study is to characterize the patients seen in a hospital in the southern sector of Santiago, Chile, identifying their social and clinical factors, classifying the main types of fracture at the level of the facial third middle. A retrospective observational study was conducted with the Emergency Care Data (DAU) of the patients attended in the Dental Service of the Emergency Unit of the Barros Luco Assistance Complex (CABL), extracting the sex, age, commune of Origin, etiology of the trauma, categorization according to the "Emergency Severity Index" evaluation time and compromised bone structures of patients with diagnosis of Fracture in the Third Facial Mid-Section. Point estimators of percentage, mean and standard deviation were used to represent the variables. The significance of the relationship of independent variables was calculated, with a value of p <0.05 statistically significant for the sample size. Mainly affected population corresponded to men versus women (2.4: 1), and between the second and third decade of life. Main etiology identified were accidents at home (n = 33), followed by assaults (n = 55). Most of the patients entered with a risk of C4 (n = 107) and C3 (n = 64). The most frequent fracture was nasal (46%), followed by an orbital complex (11.3 %) and a zygomatic arch (7.9 %). It is necessary to keep the epidemiology of TMF up to date considering the complexity of its early management and morbidities in the medium and long term.

Comparative analysis and characterization of soluble factors and exosomes from cultured adipose tissue and bone marrow mesenchymal stem cells in canine species.

The two main sources of mesenchymal stem cell (MSCs) in the canine species are bone marrow (cBM-MSCs) and adipose tissue (cAd-MSCs). The secretion of multitude bioactive molecules, included under the concept of secretome and found in the cultured medium, play a predominant role in the mechanism of action of these cells on tissue regeneration. Although certain features of its characterization are well documented, their secretory profiles remain unknown. We described and compared, for the first time, the secretory profile and exosomes characterization in standard monolayer culture of MSCs from both sources of the same donor as well as its immunomodulatory potential. We found that despite the similarity in surface immunophenotyping and trilineage differentiation, there are several differences in terms of proliferation rate and secretory profile. cAd-MSCs have advantages in proliferative capacity, whereas cBM-MSCs showed a significantly higher secretory production of some soluble factors (IL-10, IL-2, IL-6, IL-8, IL-12p40, IFN-γ, VEGF-A, NGF-ß, TGF-ß, NO and PGE2) and exosomes under the same standard culture conditions. Proteomics analysis confirm that cBM-MSCs exosomes have a greater number of characterized proteins involved in metabolic processes and in the regulation of biological processes compared to cAd-MSCs. On the other hand, secretome from both sources demonstrate similar immunomodulatory capacity when tested in mitogen stimulated lymphocyte reaction, but not in their exosomes at the dose used. Considering that the use of secretome open as a new therapeutic strategy for different diseases, without the need to implant cells, those biological differences should be considered, when choosing the MSCs source, for either cellular implantation or direct use of secretome for a specific clinical application.

PERMANENT GENETIC RESOURCES: Development of microsatellite markers for the ecosystem bioengineer mussel Perumytilus purpuratus and cross-priming testing in six Mytilinae genera.

Eight microsatellite markers have been characterized from the Perumytilus purpuratus genome. Their gene diversity ranged from 0.057 to 0.873 and significant interpopulation genic heterogeneity was observed between two populations of southeastern Pacific (Chile) and southwestern Atlantic (Argentine). Distinct cross-priming amplification rates were recovered on nine additional species belonging to six Mytilinae genera. The microsatellites developed herein would likely be a powerful intraspecific genetic tool to undertake fine population studies in the intertidal ecosystem bioengineer P. purpuratus along the South American shoreline.

Análisis de la interfase diente restauración en obturaciones con Ariston pHc / Analysis of the dentin restoration interphase in obturation with Ariston pHc

En el presente trabajo se analizó la interfase dentina-restauración en restauraciones con ariston PHC, un nuevo sistema de resina compuesta que se usa sin grabado ácido previo y que está indicado para dientes posteriores. En molares recientemente extraídos se confeccionaron 3 restauraciones, una siguiendo las instrucciones de uso del fabricante, otra agregando a esta técnica el uso de grabado ácido previo, y la tercera con grabado ácido y un adhesivo dentinario. Las restauraciones realizadas según esta técnica indicada por el fabricante presentaron brechas en las paredes pulpares y laterales de las cavidades, con su superficie interna rugosa e irregular, lo que sugiere la permanencia del barrio dentario después de la aplicación del liner. con el uso del grabado ácido total de la preparación cavitaria, las brechas laterales disminuyen de amplitud y en la pared pulpar no se desarrollan. En la superficie pulpar de las restauraciones aparecen estructuras de microrretención en forma de tags dentinarios y regiones de infiltración de resina en la dentina correspondiente a la zona híbrida. Los resultados sugieren que el grabado ácido total podría aumentar la retención de las restauraciones con ariston PHC mediante el desarrollo de estructuras de microrretención, aunque el posible uso clínico de tal procedimiento requiere descartar los posibles efectos tóxicos del liner ariston sobre la pulpa dentaria

[Ibopamine in the treatment of congestive heart failure. Multicenter follow-up study]. / A ibopamina no tratamento da insuficiência cardíaca congestiva. Estudo multicêntrico prolongado.

OBJECTIVE: To assess safety and efficacy of ibopamine, 200 mg TID, added to conventional treatment of congestive heart failure. DESIGN: A prospective, longterm, open study over two years (1986-88). A multicenter trial. SETTING: Outpatients of Departments of Internal Medicine of S. Francisco Xavier Hospital and Sta. Maria Hospital, and Departments of Cardiology of Sta. Cruz Hospital and Hospital Militar Principal, Lisbon. PATIENTS AND METHODOLOGY: 63 patients, 49 males from 34 to 80 years (m = 55.6 +/- 11.36) and 14 females from 41 to 80 years (m = 63 +/- 10.2), with congestive heart failure, NYHA class II in 52 patients (82.5%) and NYHA class III in 11 patients (17.5%) with a mean disease duration of 47.9 months entered into the study. Digoxin, diuretics, nitrates and antiarrhythmic drugs were allowed as concomitant therapy. Patients carried out clinical examination, ECG and laboratory tests monthly and X-Ray at the beginning and at the end of each year of the study. RESULTS: 42 patients completed one year of treatment and 20/42 continued for an additional year, 17 patients completed this second year of follow-up. From the 42 patients who completed the first year period, the NYHA class changed from II to I in 17/38 from II to III in 2/38 patients from III to II in 3/4 patients, and from III to IV in one patient. Twenty patients dropped during the first year of treatment. Six for non-compliance (less than 80% of the treatment). Two were submitted to cardiac valve surgery. Seven had cardiovascular clinical events: one ventricular tachycardia, one atrial fibrillation, one pulmonary edema, one patient had no therapeutic effect, two patients had anxiety and fatigue and one patient died suddenly. One diabetic patient had uncontrolled hyperglycemia. One patient had gastric ulcer. Two had nausea and vomiting. Dysrhythmia and nausea and vomiting were the only clinical events, considered, respectively, possibly related and related, to ibopamine. During the second year of treatment 9/11 patients were stabilized in NYHA class I and 6/9 in NYHA class II, one patient changed from class II to class I, and one patient changed from class I to class II of the NYHA. Three patients did not complete the second year of treatment; one due to abnormal creatininemia; one for probable pulmonary embolism with CHF worsening; the third died suddenly. None of these events was considered related to ibopamine. Heart rate, arterial pressure, laboratory values and cardiothoracic index did not vary over the two years of the study. CONCLUSIONS: This has been the first study with data from patients treated with 200 mg TID of ibopamine during two years. Ibopamine has been shown to be a safe and useful drug added to conventional treatment of cardiac heart failure. Clinical events were few and well controlled after ibopamine interruption.

True histiocytic lymphoma: histopathologic, immunophenotypic and genotypic analysis.

Five cases of true histiocytic lymphoma (THL) were analysed by immunophenotyping and immunoglobulin/T-cell receptor genotyping. These cases showed striking morphologic diversity but a strong degree of immunophenotypic homogeneity. The malignant cells reacted with multiple histiocytic markers including CD11c (Ki-M1, LeuM5), CD14, CD68 (Ki-M6) and Ki-M8; anti-HLA-DR and non-specific esterase staining was also found in all cases. The malignant cells did not express monoclonal immunoglobulin and did not react with the B- or T-cell monoclonal antibodies used except for those known to be cytoplasmically expressed in monocytes/histiocytes, such as CD4 and CD19; B- and T-cell staining was otherwise limited to background small lymphocytes. By genotypic analysis, three cases showed rearrangements: one with T beta, one with T beta and immunoglobulin heavy chain (JH) and one with both JH and light chain; the remaining two cases retained their immunoglobulin and T-cell receptor genes in germline configuration. The results not only suggest that certain subsets of the histiocyte/reticulum cell system may be capable of rearranging immunoglobulin or T beta genes while simultaneously expressing multiple histiocytic surface antigens but also demonstrate the necessity of using multiple histiocytic-specific monoclonal antibodies and cytochemical staining in diagnosing THL. Gene rearrangement studies must be interpreted in conjunction with immunophenotyping and morphology in the determination of cell lineage.

[Prevalence of anemia in a sample of school children in Durango City]. / Prevalencia de anemia en una muestra de escolares de la ciudad de Durango.

The circulatory levels of hemoglobin and the frequency of anemia were determined in a representative sample of children with a mean age of 6.7 years. A random sample of 500 children of the city of Durango was studied. Durango has an altitude of 1893 m above sea level. The mean value of hemoglobin was 12.57 g/100 ml, with a range of 9.4 to 15.0 and a SE of 0.05. There was no difference statistically significant between the sexes. Considering 12.0 as the lower normal limit for hemoglobin values, it was found that 16% of the children were anemic, 49% of the anemic children were iron deficient, with iron serum levels of less than 60 ug/100 ml. The relationship between anemia and number of living children, birth order and weight was established. The frequency of anemia was higher among children coming from families with 4 or more living children.