RESUMO
Surfactant protein A (SP-A) and Surfactant protein D (SP-D) glycoproteins play a crucial role in maintaining lung homeostasis and lung host defense. Interestingly, these proteins are also expressed in extra-pulmonary tissues, including the female genital tract. The ovarian tissue, where SP-A and SP-D expression increases with follicular development, may serve as the primary site of defense for this tissue. However, their functions in these tissues are not well understood and are currently an active area of research. Therefore, the objective of this study is to investigate the expression of SP-A and SP-D in the ovine ovary throughout the ovarian cycle using immunohistochemistry by semiquantitative intensity classification and Western blotting techniques. These findings revealed the presence of SP-A and SP-D in various compartments of the ovary, such as the follicular epithelium, granulosa cells, cumulus cells, theca cells, oocyte I, follicular fluid, and luteal cells of Graafian follicles, excluding the corpus albicans. SP-A and SP-D likely act as a first line of defense against potential pathogens that infiltrate the ovaries. Further investigation of the differential expression of SP-A and SP-D proteins in ovarian follicles will provide a basis for understanding their interactions with key proteins involved in oogenesis.
Assuntos
Folículo Ovariano , Ovário , Proteína A Associada a Surfactante Pulmonar , Proteína D Associada a Surfactante Pulmonar , Animais , Feminino , Ovinos , Proteína A Associada a Surfactante Pulmonar/metabolismo , Proteína A Associada a Surfactante Pulmonar/genética , Folículo Ovariano/metabolismo , Proteína D Associada a Surfactante Pulmonar/metabolismo , Proteína D Associada a Surfactante Pulmonar/genética , Ovário/metabolismo , Imuno-Histoquímica/veterináriaRESUMO
This study aimed to assess the possible association between cognitive impairment and two important biochemical biomarkers of oxidative stress, thiol-disulfide homeostasis (TDH), and ischemia-modified albumin (IMA) in patients with multiple sclerosis (MS). This study included 85 patients with MS (38 treatment-naïve relapsing-remitting MS (RRMS), 31 RRMS on fingolimod therapy, and 16 secondary progressive MS (SPMS)) and 33 healthy controls. Cognitive evaluation was carried out by applying the Brief International Cognitive Assessment for Multiple Sclerosis (BICAMS) test battery and the scores were adjusted for age and years of education. Plasma TDH was assessed using an automated method and plasma IMA levels were determined using the cobalt-albumin binding assay. Plasma native thiol and total thiol levels were significantly decreased in patients with SPMS when compared with the naïve patients and healthy controls. Cognitive impairment was detected in 47.4% of naïve patients, 64.5% of patients on fingolimod therapy, and 80% of patients with SPMS. Naïve patients or patients on fingolimod therapy who were cognitively impaired had significantly decreased levels of native thiol and total thiol compared to the cognitively normal patients. Logistic regression analysis revealed total thiol and native thiol to be significantly associated with cognitive impairment in naïve patients and patients on fingolimod therapy. Significant correlations were determined between BICAMS scores, TDH, IMA, clinical indices of disease severity (EDSS and MSSS), and magnetic resonance imaging parameters. This study has shown for the first time that plasma TDH parameters are associated with cognitive impairment in MS.
Assuntos
Esclerose Múltipla , Humanos , Esclerose Múltipla/complicações , Biomarcadores , Cloridrato de Fingolimode , Dissulfetos , Compostos de Sulfidrila , Albumina Sérica , Homeostase , Testes NeuropsicológicosRESUMO
This study investigated the short-term storage of Honamli and Hair buck semen based on the relationships among the biochemical constituents of the seminal plasma. In total, 12 Honamli and Hair goat bucks (six of each breed) were used. Collected individual semen samples were divided into two parts, one placed in short-term storage and the other centrifuged to determine the biochemical constituents of the seminal plasma. Diluted semen samples were cooled to +4°C; the samples were stored at this temperature; the spermatological parameters (i.e., motility, plasma membrane and acrosome integrity [PMAI], viability and high mitochondrial membrane potential) were assessed at 0, 24, 48, 72 and 144 h. A significant positive correlation was determined between the PMAI, motility and viability with globulin, chlorine, alanine aminotransferase (AST) and aspartate aminotransferase (ALT). A positive correlation was detected between the PMAI and viability with total protein. In contrast, a negative correlation was determined between the motility and viability with sodium values in buck semen (p Ë .05). Also, it was evaluated the Hair buck samples became inviable across all parameters at 72 h, while the Honamli buck samples continued to be viable at 144 h. In conclusion, the seminal plasma biochemical parameters of total protein chlorine, globulin, albumin, sodium, AST and ALT correlated with spermatological parameters in buck semen. The factors of animal (p Ë .05) and time (p Ë .001) affected the spermatological parameters, but there was no interaction between the two factors (p > .05) during the short-term storage period. Also, it was determined that Honamli buck semen was more resistant to cold injury than Hair buck semen against cold injury during storage.
Assuntos
Lesão por Frio , Doenças das Cabras , Preservação do Sêmen , Masculino , Animais , Sêmen , Espermatozoides , Preservação do Sêmen/veterinária , Cabras , Cloro , Motilidade dos Espermatozoides , Lesão por Frio/veterináriaRESUMO
The present study investigated the protective effect of dried white Mulberry extract (DWME) against carmustine (Crm) induced biochemical alterations and spermatological, histopathological, and fertility damage in Wistar albino rats. Male rats were divided into four groups (control, Crm, Crm + DWME, and DWME group). It was found that Crm decreased the motility. Crm decreased the concentration (not different from control group) compared to DWME groups. Total blood MDA levels were reduced during the recovery period. Also, the recovery period reduced the MDA levels in the Crm group/testicular tissue. The GSH levels in the Crm + DWME group were the highest among all groups in the testicular tissue/experiment period. In the immunohistochemical evaluation of the testicular tissue, a high level of caspase-3 was observed in the cells that underwent meiosis in the Crm group. The most pronounced DNA damage was also detected in the Crm group. The Crm + DWME group showed the highest number of offspring born during recovery period. In conclusion, dried white mulberry extract protects against the spermatological damages caused by carmustine. Moreover, recovery period played a positive effect on spermatological parameters and fertility.
Assuntos
Morus , Testículo , Animais , Antioxidantes/farmacologia , Carmustina/metabolismo , Carmustina/toxicidade , Fertilidade , Masculino , Estresse Oxidativo , Extratos Vegetais/metabolismo , Extratos Vegetais/farmacologia , Ratos , Ratos Wistar , EspermatozoidesRESUMO
The aim of this study was to determine the effects of gallic acid (GA) on frozen-thawed goat spermatozoa. Four Honamli goat bucks were used at their breeding season, and ejaculates were collected by an electroejaculator. Mixed semen was divided into the following four groups: control (0 mM), low (L; 1 mM), medium (M; 2 mM), and high (H; 4 mM) concentration of GA. All the groups were frozen and thawed in a water bath for spermatological evaluation. The lowest motility was observed in the control group (47.60 ± 5.70%) (P < 0.05), while the highest viability (62.45 ± 1.68%), plasma membrane and acrosome integrity (44.81 ± 4.57%), and high mitochondrial membrane potential (35.96 ± 2.50%) were observed in the low GA group (P < 0.05). Also, the lowest hypo-osmotic swelling test (HOS +) value was found in the high GA group (47.60 ± 4.82%) (P < 0.05). In conclusion, supplementing a low concentration (1 mM) of GA to the Tris-based semen extender had a positive effect on spermatological parameters after freeze-thawing of Honamli goat semen. Further studies should be continued in other species with different doses and combinations using commercial and/or homemade semen extenders.
Assuntos
Preservação do Sêmen , Sêmen , Animais , Criopreservação/veterinária , Ácido Gálico/farmacologia , Cabras , Masculino , Potencial da Membrana Mitocondrial , Preservação do Sêmen/veterinária , Motilidade dos Espermatozoides , EspermatozoidesRESUMO
The aim of the present study was to determine the possible relationship between bovine viral diarrhoea (BVD) virus infection and the appearance of cervical mucous discharge (CMD) and the reproductive performance of cows in oestrus. For this purpose, CMD from 97 Holstein cows in oestrus was evaluated visually before artificial insemination (AI). Cows in oestrus were inseminated with frozen semen free from BVD virus (BVDV). Blood samples were tested by enzyme-linked immunoassay (ELISA) for antigen (Ag) and antibodies (Ab) of BVDV. The presence of the BVDV genome in cervical mucus samples was tested by reverse transcriptase-polymerase chain reaction (RT-PCR). The presence of BVDV Ab, Ag or genome was not associated with abnormal cervical mucous discharge (A-CMD). However, the presence of BVDV Ag (but not of the BVDV Ab) in blood samples was associated with a lower first service conception rate (FSCR; 27.8 vs. 70.9%; P < 0.01), indicating that BVDV viraemia at the time of AI has a negative effect on the fertility of cows.
Assuntos
Anticorpos Antivirais , Doença das Mucosas por Vírus da Diarreia Viral Bovina , Animais , Anticorpos Antivirais/sangue , Bovinos , Diarreia , Vírus da Diarreia Viral Bovina , Ensaio de Imunoadsorção Enzimática/veterinária , Feminino , FertilidadeRESUMO
This study has been carried out in the Western Mediterranean Region of Turkey on seven different Honamli goat flocks. The aim of this study was to determine the growth characters, survival rate (till weaning, 105th day), some reproductive characteristics and marketable milk yield. The data were collected from 516 does and 210 kids (125 buckling and 85 doelings). The results indicate that the numbers of multiple birth and litter size were 32.8 % and 1.35 respectively. Survival rates of kids at the 30th, 90th, and 105th days were 98.5, 97.2, and 97.2 %, respectively. The average birth weight for the buckling and doelings was 4.1 and 3.7 kg, respectively. The average weaning weights for buckling and doelings were 26.9 and 23.4 kg, respectively. The effects of dams' age, on sex and birth type on the growth of Honamli kids were statistically significant (p < 0.05, p < 0.01, and p < 0.001). While the diameters of erythrocytes of Honamli goats are smaller, the density of hemoglobin in the erythrocytes is more than Asian, Indian, and American caprine breeds. The average lactation length and marketable milk yield was assessed to be at 210.3 days and was 89 L, respectively. The growth characteristics of Honamli kids at weaning age were assessed to be higher than most of the other goat breeds in Turkey. The results further indicated that there is a significant variation in the production traits among the different Honamli goat flocks. Production traits of Honamli goats at different breeding conditions should further be investigated to come to a definite conclusion.
Assuntos
Cruzamento , Cabras/fisiologia , Leite/metabolismo , Animais , Peso ao Nascer , Peso Corporal , Feminino , Cabras/genética , Cabras/crescimento & desenvolvimento , Testes Hematológicos/veterinária , Lactação , Tamanho da Ninhada de Vivíparos , Masculino , Reprodução , Turquia , DesmameRESUMO
In this study, blood serum and leukocyte samples were collected from 400 Holstein heifers, all of which appeared to be healthy. Antibodies (Ab) against bovine viral diarrhea virus (BVDV) were detected in 57 serum samples, and BVDV antigen (Ag) was detected in 38 leukocyte samples. There were statistically important differences between the average first insemination ages (FIT) of the BVDV (Ag-/Ab+) heifers (p<0.0001) (pregnant p<0.05, nonpregnant p<0.0001) and BVDV (Ag-/Ab-) heifers. The average conception rates (CR) of BVDV (Ag-/Ab+) heifers and BVDV (Ag-/Ab-) heifers were not significant statistically. There were statistically important differences in average FIT between persistent infected (PI) BVDV (Ag+/Ab-) heifers (p<0.0001; PI pregnant p<0.05, PI nonpregnant p<0.0001) and BVDV (Ag-/Ab-) heifers. No significant differences in average CR between PI BVDV (Ag+/Ab-) heifers and BVDV (Ag-/Ab-) heifers were found. The differences in average FIT between BVDV (Ag+/Ab+; p<0.0001; nonpregnant p<0.0001) and BVDV (Ag-/Ab-) heifers were important statistically. Although there were no BVDV (Ag+/Ab+) pregnant heifers, the differences in average CR between BVDV (Ag+/Ab+) pregnant heifers and BVDV (Ag-/Ab-) heifers were found to be statistically important (p<0.0001). We conclude that fertility is affected in heifers with BVDV (Ag-/Ab+, Ag+/Ab- and Ag+/Ab+).
Assuntos
Doença das Mucosas por Vírus da Diarreia Viral Bovina/complicações , Vírus da Diarreia Viral Bovina , Infertilidade Feminina/veterinária , Animais , Anticorpos Antivirais/sangue , Doença das Mucosas por Vírus da Diarreia Viral Bovina/sangue , Doença das Mucosas por Vírus da Diarreia Viral Bovina/imunologia , Doença das Mucosas por Vírus da Diarreia Viral Bovina/virologia , Bovinos , Feminino , Infertilidade Feminina/sangue , Infertilidade Feminina/imunologia , Infertilidade Feminina/virologia , GravidezRESUMO
OBJECTIVE: To investigate the presence of surfactant protein-A (SP-A); molecular weight 34 kDa and surfactant protein-D (SP-D); and molecular weight 43 kDa in human spermatozoa. DESIGN: Prospective, research study. SETTING: Two universities in Turkey. PATIENT(S): Semen specimens (n = 10) were obtained from normozoospermic donors. INTERVENTION(S): Human sperm were exposed to an anti-human SP-A polyclonal antibody, and monoclonal antibody, to human SP-D protein. MAIN OUTCOME MEASURE(S): Presence of SP-A and SP-D proteins in human beings. RESULT(S): Indirect immunofluorescence assays of human sperm indicated the presence of SP-A in the mid piece, the tail, and sometimes at the equatorial region of spermatozoa. A brilliant green light detected SP-D in the tails and acrosome of some sperm. The anti-SP-A antibody detected a single band corresponding to the molecular weight values of 34 kDa in spermatozoa, whereas no band was observed in the negative control. The anti-SP-D antibody showed the expected band at 43 kDa in spermatozoa. CONCLUSION(S): This is the first report and a novel finding of the presence of surfactant glycoproteins on human spermatozoa.
Assuntos
Proteína A Associada a Surfactante Pulmonar/análise , Proteína D Associada a Surfactante Pulmonar/análise , Espermatozoides/química , Acrossomo/química , Western Blotting , Técnica Indireta de Fluorescência para Anticorpo , Humanos , Masculino , Estudos Prospectivos , Peça Intermédia do Espermatozoide/química , Cauda do Espermatozoide/química , TurquiaRESUMO
The presence of surface-active material in the lung alveolus has been known for several decades as being essential for normal lung function. Surfactant is essential for reducing the surface tension at the alveolar air-liquid interface. Pulmonary surfactant is composed of 90% lipids and 10% proteins. There are four non-serum proteins surfactant protein-A (SP-A), surfactant protein-B (SP-B), surfactant protein-C (SP-C) and surfactant protein-D (SP-D) named in chronologic order of discovery. Lung SP-A and SP-D belong to a family of collagen-containing C-type lectin family called collectins. The host defence and controlling inflammatory processes of the lung are the major functions of SP-A and SP-D. SP-A and SP-D were originally demonstrated in alveolar type II cells, but recent studies have shown extrapulmonary expression of SP-A and SP-D indicating systemic roles of these proteins. Present study describes the presence of SP-A and SP-D in the mare genital tract, vulva, vagina, ovarium, uterus and tuba uterina using immunohistochemistry and Western blotting. The aim of this study was to characterize surfactant proteins in terms of: (i) whether surfactant proteins were present in the various structures of the mare genital system, (ii) if so, identifying and locating the surfactant proteins and finally (iii) determining the differences from those previously characterized for the lung. Although beyond the scope of this report, it is recognized that there are also some potential implications for better defining the reproductive defence mechanisms in mare. Therefore, genital system organs and tissues from mares were examined. We were able to show that proteins reactive with surfactant-specific antibodies were present in the mare genital tract. Thus, surfactant proteins are present not in just lamellar bodies associated with lung, but also genital system of mare.
Assuntos
Genitália Feminina/química , Cavalos/metabolismo , Proteína A Associada a Surfactante Pulmonar/análise , Proteína D Associada a Surfactante Pulmonar/análise , Animais , Western Blotting , Eletroforese em Gel Bidimensional , Feminino , ImunoquímicaRESUMO
The presence of surface-active material in the lung alveolus has been known for several decades as being essential for normal lung function. The host defense and controlling inflammatory processes of the lung are the major functions of SP-A and SP-D. SP-A and SP-D were originally demonstrated in alveolar type II cells, but recent studies have shown extrapulmonary expression of SP-A and SP-D indicating systemic roles of these proteins. Present study describes the presence of SP-A and SP-D in the stallion genital tract, prepuce, prostate, testis, and seminal vesicle using Western blotting and immunohistochemistry. This paper presents the first evidence for the existence of SP-A and SP-D glycoproteins in the stallion genital tract. We examined genital system organs and tissues from stallion and were able to show that surfactant protein A and D reactive with surfactant-specific antibodies were present in the stallion genital tract tissues and organs. On the basis of results, it can be postulated that surfactant proteins in the stallion reproductive tract contribute to the immune surveillance and to active barrier defense mechanism.