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1.
Plant Physiol ; 164(4): 1952-66, 2014 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-24532604

RESUMO

KNOTTED1-LIKE HOMEOBOX (KNOX) genes are important regulators of meristem function, and a complex network of transcription factors ensures tight control of their expression. Here, we show that members of the GROWTH-REGULATING FACTOR (GRF) family act as players in this network. A yeast (Saccharomyces cerevisiae) one-hybrid screen with the upstream sequence of the KNOX gene Oskn2 from rice (Oryza sativa) resulted in isolation of OsGRF3 and OsGRF10. Specific binding to a region in the untranslated leader sequence of Oskn2 was confirmed by yeast and in vitro binding assays. ProOskn2:ß-glucuronidase reporter expression was down-regulated by OsGRF3 and OsGRF10 in vivo, suggesting that these proteins function as transcriptional repressors. Likewise, we found that the GRF protein BGRF1 from barley (Hordeum vulgare) could act as a repressor on an intron sequence in the KNOX gene Hooded/Barley Knotted3 (Bkn3) and that AtGRF4, AtGRF5, and AtGRF6 from Arabidopsis (Arabidopsis thaliana) could repress KNOTTED-LIKE FROM ARABIDOPSIS THALIANA2 (KNAT2) promoter activity. OsGRF overexpression phenotypes in rice were consistent with aberrant meristematic activity, showing reduced formation of tillers and internodes and extensive adventitious root/shoot formation on nodes. These effects were associated with down-regulation of endogenous Oskn2 expression by OsGRF3. Conversely, RNA interference silencing of OsGRF3, OsGRF4, and OsGRF5 resulted in dwarfism, delayed growth and inflorescence formation, and up-regulation of Oskn2. These data demonstrate conserved interactions between the GRF and KNOX families of transcription factors in both monocot and dicot plants.


Assuntos
Arabidopsis/metabolismo , Hordeum/metabolismo , Oryza/metabolismo , Proteínas de Plantas/metabolismo , Fatores de Transcrição/metabolismo , DNA de Plantas/metabolismo , Regulação para Baixo/genética , Regulação da Expressão Gênica de Plantas , Inativação Gênica , Glucuronidase/metabolismo , Especificidade de Órgãos/genética , Oryza/genética , Oryza/ultraestrutura , Fenótipo , Folhas de Planta/metabolismo , Folhas de Planta/ultraestrutura , Proteínas de Plantas/química , Raízes de Plantas/crescimento & desenvolvimento , Brotos de Planta/crescimento & desenvolvimento , Regiões Promotoras Genéticas/genética , Ligação Proteica , Estrutura Terciária de Proteína , Saccharomyces cerevisiae/metabolismo , Fatores de Transcrição/genética , Técnicas do Sistema de Duplo-Híbrido , Regulação para Cima
2.
Toxicol Sci ; 125(1): 285-98, 2012 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-22003191

RESUMO

People are exposed to an ever-increasing number of chemical compounds that are developed by industry for a wide range of applications. These compounds may harmfully react with different cellular components and activate specific defense mechanisms that provide protection against the toxic, mutagenic, and possibly oncogenic consequences of exposure. Monitoring the activation of specific cellular signaling pathways upon exposure may therefore allow reliable and mechanism-based assessment of potential (geno)toxic properties of chemicals, while providing insight into their primary mode of toxicity. By whole-genome transcription profiling of mouse embryonic stem cells, we identified genes that were transcriptionally activated upon exposure to either genotoxic compounds or pro-oxidants. For selected biomarker genes, we constructed reporters encoding C-terminal green fluorescent protein (GFP)-tagged fusion proteins. GFP reporter genes were located on bacterial artificial chromosomes, thereby enabling transcriptional regulation of the reporters by their own physiological promoter. The Bscl2-GFP reporter is selectively activated after exposure to genotoxic agents and its induction is associated with inhibition of DNA replication and activation of the ataxia telangiectasia and Rad3-related protein signaling pathway. The Srxn1-GFP reporter is preferentially induced upon oxidative stress and is part of the nuclear factor (erythroid-derived 2)-like 2-antioxidant response pathway. The novel (geno)toxicity assay (ToxTracker) that utilize the differential responsiveness of various reporter cell lines will enable prediction of the primary reactive properties of known and unknown chemicals.


Assuntos
Dano ao DNA , Células-Tronco Embrionárias , Genes Reporter , Proteínas de Fluorescência Verde/genética , Testes de Mutagenicidade/métodos , Mutagênicos/toxicidade , Animais , Biomarcadores , Western Blotting , Linhagem Celular , Citometria de Fluxo , Subunidades gama da Proteína de Ligação ao GTP , Perfilação da Expressão Gênica , Proteínas Heterotriméricas de Ligação ao GTP/genética , Camundongos , Microscopia de Fluorescência , Mutagênicos/química , Estresse Oxidativo/efeitos dos fármacos , Estresse Oxidativo/genética , Oxirredutases atuantes sobre Doadores de Grupo Enxofre/genética , Reação em Cadeia da Polimerase em Tempo Real , Proteínas Recombinantes de Fusão/genética , Reprodutibilidade dos Testes , Transfecção
3.
Mutat Res ; 709-710: 49-59, 2011 May 10.
Artigo em Inglês | MEDLINE | ID: mdl-21382384

RESUMO

Various in vitro test systems have been developed for genotoxic risk assessment in early drug development. However, these genotoxicity tests often show limited specificity, and provide limited insights into the mode of toxicity of the tested compounds. To identify genes that could serve as specific biomarkers for genotoxicity or oxidative stress, we exposed mouse embryonic stem (ES) cells to various genotoxic and oxidative stress-inducing compounds and performed genome-wide expression profiling. Differentially expressed genes were classified based on the fold-change of expression and their specificity for either genotoxic or oxidative stress. Promoter regions of four selected genes (Ephx1, Btg2, Cbr3 and Perp) were fused to a DsRed fluorescent reporter gene and stably integrated in mouse ES cells. Established stable reporter cell lines displayed significant induction of DsRed expression upon exposure to different classes of genotoxic and oxidative stress-inducing compounds. In contrast, exposure to non-genotoxic carcinogenic compounds did not induce DsRed expression even at cytotoxic doses. Expression of the Cbr3-DsRed reporter was more responsive to compounds that induce oxidative stress while the other three DsRed reporters reacted more specific to direct-acting genotoxic agents. Therefore, the differential response of the Btg2- and Cbr3-DsRed reporters can serve as indicator for the main action mechanism of genotoxic and oxidative stress-inducing compounds. In addition, we provide evidence that inhibition of DNA replication results in preferential activation of the Btg2-DsRed genotoxicity reporter. In conclusion, we have generated sensitive mouse ES cell reporter systems that allow detection of genotoxic and oxidative stress-inducing properties of chemical compounds and can be used in high-throughput assays.


Assuntos
Dano ao DNA , Células-Tronco Embrionárias , Genes Reporter , Proteínas Luminescentes/genética , Testes de Mutagenicidade/métodos , Estresse Oxidativo , Animais , Biomarcadores , Carcinógenos/toxicidade , Linhagem Celular , Replicação do DNA , Perfilação da Expressão Gênica , Camundongos , Camundongos Endogâmicos C57BL , Mutagênicos/toxicidade , Medição de Risco
4.
Plant Physiol ; 147(3): 1347-57, 2008 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-18467450

RESUMO

Plant defense against pathogens depends on the action of several endogenously produced hormones, including jasmonic acid (JA) and ethylene. In certain defense responses, JA and ethylene signaling pathways synergize to activate a specific set of defense genes. Here, we describe the role of the Arabidopsis (Arabidopsis thaliana) APETALA2/ETHYLENE RESPONSE FACTOR (AP2/ERF) domain transcription factor ORA59 in JA and ethylene signaling and in defense. JA- and ethylene-responsive expression of several defense genes, including PLANT DEFENSIN1.2 (PDF1.2), depended on ORA59. As a result, overexpression of ORA59 caused increased resistance against the fungus Botrytis cinerea, whereas ORA59-silenced plants were more susceptible. Several AP2/ERF domain transcription factors have been suggested to be positive regulators of PDF1.2 gene expression based on overexpression in stably transformed plants. Using two different transient overexpression approaches, we found that only ORA59 and ERF1 were able to activate PDF1.2 gene expression, in contrast to the related proteins AtERF1 and AtERF2. Our results demonstrate that ORA59 is an essential integrator of the JA and ethylene signal transduction pathways and thereby provide new insight into the nature of the molecular components involved in the cross talk between these two hormones.


Assuntos
Proteínas de Arabidopsis/metabolismo , Arabidopsis/imunologia , Ciclopentanos/metabolismo , Etilenos/metabolismo , Imunidade Inata , Oxilipinas/metabolismo , Fatores de Transcrição/metabolismo , Arabidopsis/metabolismo , Arabidopsis/microbiologia , Botrytis/fisiologia , Defensinas/metabolismo , Regulação da Expressão Gênica de Plantas , Genoma de Planta , Interações Hospedeiro-Patógeno , Doenças das Plantas , Interferência de RNA , Transdução de Sinais
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