Metabolomic approaches to explore chemodiversity in seeds of guaraná (Paullinia cupana) using UPLC-QTOF-MSE and NMR analysis.

The growing interest in health and well-being has spurred the evolution of functional foods, which provide enhanced health benefits beyond basic nutrition. Guaraná seeds (Paullinia cupana) have been widely studied and used as a functional food due to their richness in caffeine, phenolic compounds, amino acids, and other nutrients. This has established guaraná as a significant food supplement, with Brazil being the largest producer of the world. This study aims to propose a set of analytical methods to chemically evaluate fifty-six different guaraná clones, from the Guaraná Germplasm Active Bank, to accommodate the diverse requirements of the food industry. Metabolomic approaches were employed, in which a non-target metabolomic analysis via UPLC-QTOF-MSE led to the annotation of nineteen specialized metabolites. Furthermore, targeted metabolomics was also used, leading to the identification and quantification of metabolites by NMR. The extensive data generated were subjected to multivariate analysis, elucidating the similarities and differences between the evaluated guaraná seeds, particularly concerning the varying concentration levels of the metabolites. The metabolomics approach based on the combination of UPLC-QTOF-MSE, NMR and chemometric tools provided sensitivity, precision and accuracy to establish the chemical profiles of guaraná seeds. In conclusion, evaluating and determining the metabolic specificities of different guarana clones allow for their application in the development of products with different levels of specific metabolites, such as caffeine. This caters to various purposes within the food industry. Moreover, the recognized pharmacological properties of the annotated specialized metabolites affirm the use of guarana clones as an excellent nutritional source.

Associação entre características da parte aérea e do sistema radicular em mudas de guaranazeiro por correlações canônicas / Canonical correlations among characteristics of shoots and roots system in guarana seedlings

Este trabalho teve como objetivo quantificar o grau de asociação entre variáveis de parte aérea e de raízes em mudas clonadas de guaranazeiro, utilizando-se correlações canônicas, a fim de aperfeiçoar o procedimento de seleção de mudas para garantir o aumento da porcentagem de sobrevivência das mudas após o plantio. Foram avaliados dois grupos de variáveis em mudas aptas ao plantio definitivo de 36 clones de guaranazeiro. O delineamento usado foi o aleatorizado em blocos com cinco repetições e 10 plantas por parcela, sob condições de viveiro. Os caracteres avaliados foram submetidos à análise de correlações canônicas. Utilizou-se a análise de correlações canônicas. O grupo de variáveis da parte aérea não se mostrou independente do grupo de variáveis do sistema radicular. Através de seleção baseada em variáveis da parte aérea pode-se melhorar o sistema radicular, principalmente através do maior comprimento do ramo (CRA). A seleção de clones de guaraná para maior peso de raiz pode ser efetuada de forma indireta, realizando-se mensurações do comprimento dos ramos, o que evita a necessidade de se destruir as mudas.

This study aimed to quantify the degree of association between variables of shoots and roots system of seedlings cloned from guarana, using canonical correlations, in order to improve the procedure of selection of seedlings to ensure increased survival percentage of seedlings after planting. Two groups of variables suitable for final planting seedlings in 36 guarana clones. The experimental design was a randomized complete block with five replications and 10 plants per plot, under nursery conditions. We used the canonical correlation analysis. The group of variables of shoot is not independent of variable group of root system. Through selection based on variables from the air, you can improve the root system, mainly through the greater length of the branch (CRA). Can practice the selection of clones of guarana for increased root weight without the need to destroy the seedlings carrying only measurements of the length of the branches.

Guaraná's Journey from Regional Tonic to Aphrodisiac and Global Energy Drink.

Guaraná (Paullinia cupana H.B.K., Sapindaceae) is a rainforest vine that was domesticated in the Amazon for its caffeine-rich fruits. Guaraná has long been used as a tonic and to treat various disorders in Brazil and abroad and became a national soda in Brazil about a century ago. In the last two decades or so, guaraná has emerged as a key ingredient in various 'sports' and energy drinks as well as concoctions that allegedly boost one's libido. For some time, guaraná's high caffeine content was thought to be a detriment because of health concerns about excessive intake of caffeine-rich drinks. But it is precisely this quality, and the fact that it has a mysterious name and comes from an exotic land, that has propelled guaraná into a global beverage.

Fertilization and cover crop effects on soil nitrogen and plant nutrition in a young guarana plantation / Efeitos da fertilização mineral e da cobertura do solo sobre a dinâmica do Nitrogênio e na nutrição de plantas jovens de guaraná

Fruit tree production is gaining an increasing importance in the central Amazon and elsewhere in the humid tropics, but very little is known about the nutrient dynamics in the soil-plant system. The present study quantified the effects of fertilization and cover cropping with a legume (Pueraria phaseoloides (Roxb.) Benth.) on soil nitrogen (N) dynamics and plant nutrition in a young guarana plantation (Paullinia cupana Kunth. (H.B. and K.) var. sorbilis (Mart.) Ducke) on a highly weathered Xanthic Ferralsol. Large subsoil nitrate (NO3-) accumulation at 0.3-3 m below the guarana plantation indicated N leaching from the topsoil. The NO3- contents to a depth of 2 m were 2.4 times greater between the trees than underneath unfertilized trees (P<0.05). The legume cover crop between the trees increased soil N availability as shown by elevated aerobic N mineralization and lower N immobilization in microbial biomass. The guarana N nutrition and yield did not benefit from the N input by biological fixation of atmospheric N2 by the legume cover (P>0.05). Even without a legume intercrop, large amounts of NO3- were found in the subsoil between unfertilized trees. Subsoil NO3- between the trees could be utilized, however, by fertilized guarana. This can be explained by a more vigorous growth of fertilized trees which had a larger nutrient demand and exploited a larger soil volume. With a legume cover crop, however, more mineral N was available at the topsoil which was leached into the subsoil and consequently accumulated at 0.3-3 m depth. Fertilizer additions of P and K were needed to increase subsoil NO3- use between trees.

A produção de fruteiras está ganhando grande importância na Amazônia Central e em outras partes dos trópicos úmidos mas, muito pouco ainda é conhecido sobre a dinâmica de nutrientes no sistema solo-planta. O presente estudo quantificou os efeitos da fertilização mineral e da cobertura do solo com uma leguminosa (Pueraria phaseoloides (Roxb) Benth.) sobre a dinâmica do N no solo e sobre a nutrição de plantas jovens de guaraná (Paullinia cupana Kunth. (H.B. and K.) var. sorbilis (Mart.) Ducke), em um Latossolo Amarelo muito argiloso. Grande acúmulo de nitrato (NO3-) encontrado na profundidade de 0,3 - 3,0m abaixo do plantio de guaraná é um indicativo da lixiviação de N da camada superficial. Os teores de NO3- na profundidade de 2m era 2,4 vezes maior entre as plantas do que na entrelinha que não recebeu fertilização (P<0,05). A leguminosa de cobertura, entre as plantas de guaraná, aumentou a disponibilidade de N, conforme é indicado pela elevada mineralização aeróbica e baixa imobilização de N na massa microbiana. A nutrição nitrogenada e a produção do guaraná não foram beneficiados pela adição de N, via fixação biológica do N2 da atmosfera pela leguminosa de cobertura (P<0,05). Mesmo sem leguminosa nas entrelinhas de plantio, grandes quantidades de NO3- foram encontradas no subsolo, entre plantas não adubadas. O NO3- do subsolo entre as plantas pode, entretanto, ter sido utilizado pelo guaraná fertilizado. Isso pode ser explicado pelo crescimento mais vigoroso das plantas fertilizadas as quais têm uma grande demanda por nutrientes e exploraram maior volume de solo. Com uma leguminosa de cobertura, contudo, mais N mineral foi disponibilizado na camada superficial, o qual foi lixiviado para o subsolo e, consequentemente, acumulado na camada de 0,3 a 3,0m de profundidade. Adições suplementares de P e K foram necessárias para aumentar a utilização do NO3- entre as plantas.