[EXISTENTIAL ORGANIZATION OF BRAIN BIOELECTRICAL ACTIVITY OF THE OFFSPRING OF RATS AFTER VITAL STRESS].

An association was found between severity of behavioral disturbances in the offspring of rats that survived the stress, and changing spatial-temporal organization of the ECOG. The proportion of synchronous activity in the right frontal and left occipital regions with respect to the right nape, and between the frontal leads increases with the minimum deviations of behavior. The proportion of ahead activity increased in frontal leads; reduced the share of lagging and an increasing proportion of synchronous bioelectrical activity in relation to the right occipital region in case of serious disorders of behavior.

[Influence of maternal factor on motor behavior and lipid turnover in rats exposed to severe psychoemotional stress].

Adverse condition of rat development during the prenatal period (rearing by mother survived brain injury) or during the early post-natal period (cross fostering), causes impairment of motor behavior and a lipid turnover in adult rats. Such rats under severe stress showed decrease of adaptation (as expressed or low indexes of motor impellent behavior and lipid metabolism) in comparison with rats that were grown up by own mother. The psychoemotional stress caused the most expressed deficient behavior in males that were grown up by mothers with brain injury (decrease in serum level of HC and HDL and depressively - similar behavior).

[Features of an existential organization of a bioelectric activity of the brain at posterity from rats with a lateral injury of the brain].

Features of the existential organization of a bioelectric activity of a brain at posterity from rats with a lateral injury of the brain were investigated. The EEG analysis revealed in one month animals significantly increased the proportion of advanced and lagging decrease in the activity of the right frontal derivation towards the right occipital region. Change between structural ties in terms of the rats were correlated with the severity of behavioral disorders in the test "open field" (OF). The most significant change in the existential organization of a bioelectrical activity (the reduction of synchronous communications between the frontal derivations) recorded in rats with severe conduct disorder compared with animals of the same groups, in which the behavior was not significantly different from the norm. The violation of the behavior in the test OF was accompanied by a decrease in synchronous activity between the left frontal and right occipital region or the decline in the anticipatory activity in the right frontal region in relation to the right occipital region. By maintaining the integrity of the behavior of these parameters were increased in comparison with an intact control.

[Peculiarities of behavior of offspring of rats-ambidexters after surviving vital stress].

Psychogenic trauma inflicted to mothers (Wistar rats) during pregnancy is more tragic for establishment of psychoemotional functions in offspring than in the rat pups whose mothers survived prior to pregnancy the stress connected with threat of life. The "antenatal stress" causes in the one-month-old offspring the depression-like behavior, while the stress one month prior to conception--the increased anxiety. Disturbances of the integral behavior in the test "open field" can be caused both by the acute psychogenic trauma of mothers and by its delayed consequences. The sharp impoverishment of behavior and the more pronounced psychoemotional disturbances are realized in female individuals regardless of the terms of the action. In the male offspring the vital stress during their mothers' pregnancy produces the rougher behavioral disturbances than stress prior to conception.

[Polyunsaturated fatty acids prevent the development of locomotor disturbances caused by changes in the neurohumoral background in rat pups].

The present study shows the effectiveness of using polyunsaturated fatty acids in pregnant rats in order to prevent behavioral alterations in neonatal rats caused by the action of the pathogenic peptide factors of blood serum with perinatal damage of the central nervous system.

[The influence of the rat maternal environment on the behavior of offspring].

In Wistar's rats, the postnatal influence of maternal behaviour on the motor and emotional state and the anxiety level of the posterity born from the intact rat-mothers-ambidexter but reared by the intact mothers-ambidexter with the motor deficit, were investigated. From the first day of life, the rat-mothers with the removed part of the sense-motor cortex (right or left) nursed and reared the litter. It was discovered that the reduction of motor activity and increase of negative emotional responses occurred in experimental litter at the age of 1 month as compared with the control group (intact litter reared by the ersatz healthy rat-mothers). In the test of elevated cruciform maze, a high level of anxiety was discovered. The experimental posterity showed an emotional tension as the dominant state. Left-side lesion of the sense-motor cortex of the ersatz mother resulted in a rougher disorder in the emotional state and movement than after right-side lesion.

[Unilateral injury of sensorimotor cortex of the dominant or subdominant hemispheres of rats-mothers and emotional behavior of their offsprings in the "open field"]. / Unilateral'noe povrezhdenie sensomotornoi kory dominantnogo i subdominantnogo polusharii mozga krys-materei i povedenie potomstva v "otkrytom pole".

Neurohumoral disorders in mothers caused by brain injury, infection, hypoxia, and other pathological factors result in motor and psychoemotional disorders in children. Emotional behavior of 30-day-old offsprings of female rats with unilateral sensorimotor brain injury was studied in the "open field". Individual behavior was estimated (the probability of certain acts and significant transfers between them). Behavioral disorders in rat offsprings depended on the side (left of right) of mother's brain injury and "handedness". The right-side mother's injury turned to be more deleterious. Behavioral alterations were stronger in offsprings of ambidextral and left-handed females than in those born by right-handed males with the same sensorimotor injury.

Lateralization of brain trauma in female Wistar rats determines the immune and neurological status of offspring.

Unilateral trauma to the sensorimotor cortex in ambidextrous maternal female rats decreased the resistance of their offspring, as indicated by a reduction in the functional activity of natural killer cells. Offspring rats showed T-cell immunodeficiency regardless of the lateralization of the trauma in their mothers; this was more severe after right-sided trauma (both mature and immature T-lymphocytes were affected). The EMG pattern of evoked responses in offspring rats changed according to the lesions evoked by unilateral trauma in their mothers (in the muscles responsible for postural asymmetry). Cruder movement disturbances were also seen in offspring after right-sided trauma in mothers. Right-sided organic damage to the sensorimotor cortex in mothers evoked marked increases in negative emotionality and decreases in the motivation of orientational-investigative behavior in one-month-old offspring rats in the "open field" test.

[Lateralisation of the brain injury in female Wistar rats determines the immune and neurological status in offspring]. / Lateralizatsiia travmy mozga u krys-samok (Wistar) opredeliaet immunnyi i nevrologicheskii status potomstva.

The T-cell immune deficiency was found to be more obvious in offspring of rats with a right-side brain injury. The EMG pattern changed in accordance with the brain injury lateralisation in the muscles responsible for posture asymmetry. The offspring EMG disorders were more obvious in cases of a right-side brain injury in mothers. The right-side brain injury in mothers led to a higher negative emotionality and a diminished tentative-research behaviour in "open-field" tests in 30-day old offspring.

[Prediction of potential motor disorders in babies from neonatal serum biological test findings]. / Prognozirovanie vozmozhnykh dvigatel'nykh narushenii u detei po rezul'tatam biotesta syvorotki krovi novorozhdennykh.

To predict motor activity in the newborn, electromyographic (on rats) and ultrastructural (on chick embryos) studies were performed after administration of donor babies' sera taken in the first days of life. Morphological and electromyographic parameters were compared with late clinical symptoms. A rating schedule was developed with regards to biological test results, which allows motor disorders to be detected in the newborn.

[The embryotoxic effect in rats exposed to the blood serum of newborn infants tested for the presence of transfer factors]. / Embriotoksicheskii éffekt u krys pri vozdeistvii syvorotkoi krovi novorozhdennykh detei, testiruemykh na nalichie transfernykh faktorov.

Pregnant rats (day 14 of gestation) received intraamniotic injections of newborn human serum. Transfer factors (TF) present in injected serum produced significant embryotoxic and teratogenic effects, as well as changes in coordination of motor activity (EMG) in newborn rats. The extent of embryological disturbances and the motor activity of 33- to 34-day old recipient rats depend on TF concentration (at serum dilution 1:2) and on the state of infant's motor functions ("normal" group and "risk" group). The results obtained allow to objectively determine the pathogenic effect of serum TF on embryotoxicity and formation of motor coordination in the donor infant.

[The locomotor and spatially oriented behavior of normal rat pups and in experimental pathology]. / Lokomotornoe i prostranstvenno-orientirovochnoe povedenie krysiat v norme i pri éksperimental'noi patologii.

Breach of locomotor functions and spatially oriented behaviour of rats' cubs after intraamniotic administration of the blood serum of children was shown to depend on the ontogenetic maturity and motor disturbances of donors. Injections were carried out on 14-th day of rats pregnancy.

[A neuromorphological analysis of the motor area of the cerebral cortex in rat pups subjected to prenatal exposure to normal and pathological blood serum]. / Neiromorfologicheskii analiz motornoi oblasti kory mozga krysiat, podvergshikhsia prenatal'nomu vozdeistviiu normal'noi i patologicheskoi syvorotki krovi.

Morphological quantitative and qualitative analysis of the pyramidal neurones in the motor cortex of rat puppies after administration of the blood serum from human donors with central motor disorders reveals the dependence of the quality and quantity of neurones from the effect of corresponding blood sera. Pathologic blood serum decreases the number and induces degradation of dendritic organization of the pyramidal neurones. The effect of the blood sera on quantitative and qualitative properties of the pyramidal neurones in rat puppies depends on the age of donor infants with central motor disorders. Blood serum from newborn babies with motor dysfunctions significantly decreases the number of labeled pyramidal neurones and results in degradation of dendritic organization, whereas sera from 11-13-year infants only insignificantly affect these characteristics.

[The effect of amniotic fluid taken from women in labor on the morphofunctional development of fetal and newborn rats]. / Vliianie amnioticheskoi zhidkosti, vziatoi u zhenshchin vo vremia rodov, na morfofunktsional'noe razvitie plodov i novorozhdennykh krys.

Amniotic fluid from women was injected intra-amniotically to rats at the 14th day of pregnancy in a dose 0.3 ml. Morphological and functional parameters of embryonic development of rats and EMG activity of rat puppies at the age of 30-34 days differed from control ones with respect to the degree of maturity and motor disturbances of newborn babies. The data obtained reveal not only evident but also masked forms of motor pathology which was observed in children later, namely to 6-9 months. Chronic motor pathology in rat puppies was unilateral with central motor disturbances in babies being directly related to the degree of these disturbances.

[Detection of movement disorders in newborn infants by using infants' blood serum in experimental models]. / Vyiavlenie dvigatel'nykh rasstroistv v period novorozhdennosti priemami biotestirovaniia syvorotki krovi detei na éksperi- mental'nykh modeliakh.

A method of early diagnosis of motor disorders of the central genesis in neonates within the first to the third days of life was elaborated in experimental models for estimating the status of the motor apparatus. The experiments were made on spinal (Th3-Th5) albino noninbred rats used as recipients, in which spontaneous and electric stimulation-induced EMG activity of the antagonists muscles of the hind limbs was recorded before and after intralumbar administration of donors' blood serum. The presence of motor pathology in a donor child is judged from the changes in the EMG parameters after administration of blood serum. The method enables revealing motor disorders in neonates even in cases where the clinical examination does not provide any conclusive answer.

[Afferent determination of transfer factor formation in the rat spinal cord]. / Afferentnaia determinatsiia obrazovaniia transfernogo faktora v spinnom mozge krys.

The mechanisms causing the appearance of transfer factor (TF) in the cerebrospinal tissue of rats were studied. Intracerebral TF injections to intact recipients were found to change asymmetrically the reflectory muscular responses. TF could be found in the extract of the rat spinal cord in rats with cut off n. tibialis sin. or with alcohol-novocaine blockade of posterior left tibial muscles, i.e., the reaction of the cerebral cells to the break in axon integrity is not responsible for TF formation. The appearance of TF in the cerebrospinal tissue less than two hours after alcohol-novocaine muscular blockade does not suggest that the axon integrity is the cause initiating TF formation. It is assumed that the deafferentation determines TF appearance in the cerebrospinal tissue.