Antimicrobial effects of microwave-induced plasma torch (MiniMIP) treatment on Candida albicans biofilms.

The susceptibility of Candida albicans biofilms to a non-thermal plasma treatment has been investigated in terms of growth, survival and cell viability by a series of in vitro experiments. For different time periods, the C. albicans strain SC5314 was treated with a microwave-induced plasma torch (MiniMIP). The MiniMIP treatment had a strong effect (reduction factor (RF) = 2.97 after 50 s treatment) at a distance of 3 cm between the nozzle and the superior regions of the biofilms. In addition, a viability reduction of 77% after a 20 s plasma treatment and a metabolism reduction of 90% after a 40 s plasma treatment time were observed for C. albicans. After such a treatment, the biofilms revealed an altered morphology of their cells by atomic force microscopy (AFM). Additionally, fluorescence microscopy and confocal laser scanning microscopy (CLSM) analyses of plasma-treated biofilms showed that an inactivation of cells mainly appeared on the bottom side of the biofilms. Thus, the plasma inactivation of the overgrown surface reveals a new possibility to combat biofilms.

Fast mode-hop-free acousto-optically tuned laser: theoretical and experimental investigations.

We developed a theory that describes fast mode-hop-free tuning of an external cavity diode laser in Littrow configuration with two acousto-optic modulators (AOMs) inside the laser cavity. The theory is based on synchronous shifting of the external cavity modes and the Littrow grating selectivity. It allows calculating the driving signals of both AOMs in order to reach a desired temporal variation of the laser frequency, including particularly fast tuning as well as an arbitrary shape of the tuning function. Furthermore, we present a laser setup for which the needed signals for both AOMs are generated by two direct digital synthesizer circuits. Thereby we were able to verify the theoretical predictions experimentally, achieving, e.g., sinusoidal single-mode tuning of the laser frequency over 40 GHz at a repetition rate of 10 kHz and over 12 GHz at 25 kHz. Finally, the limitations of the theory are discussed.

Fast mode-hop-free acousto-optically tuned laser with a simple laser diode.

A mode-hop-free tunable external-cavity Littrow diode laser with intracavity acousto-optic modulators (AOMs) has been built. The modes of the red laser diode without a special antireflection coating are shifted by varying the injection current. The external resonator modes and the grating selectivity are independently electrically alterable by two AOMs. Thus, a tuning of the external resonator over up to 1900 GHz is possible. A precise computer control of laser diode and AOMs allowed a single-mode tuning of the whole laser with a tuning range of 225 GHz in 250 s. Additionally, we demonstrated fast tuning over 90 GHz in 190 micros and a repetition rate of 2.5 kHz.