Evaluation of gases emission and enzyme dynamics in sheep manure compost occupying with peach shell biochar.

The effect of peach shell biochar (PSB) amendment on sheep manure (SM) composting was investigated. Five different ratios of PSB were applied (0%, 2.5%, 5%, 7.5%, and 10% PSB), and named T1 to T5, and run 50 days of composting experiment. It was found that PSB (especially 7.5% and 10%) could improve the compost environment, regulate the activity of microorganisms and related enzymes, and promote the decomposition of compost. 7.5% and 10% PSB advanced the heap into the thermophilic stage and increased the maximum temperature, while also increasing the germination index by 1.40 and 1.39 times compared to control. Importantly, 10% PSB effectively retained more than 60% of carbon and 55% of nitrogen by inhibiting the excess release of NH3 and greenhouse gases. High proportion PSB amendment increased the activity of dehydrogenase and cellulase, but inhibited protease and urease. The correlation results indicated that PSB changed the key bacterial genus, and there was a stronger association with environmental factors at 7.5% and 10%. Therefore, 7.5% and 10% peach shell biochar can be used as appropriate proportions to improve composting conditions.

Facile green synthesis of novel tantalum doped tungsten trioxide for photocatalytic degradation: Correlation with COMSOL simulation.

The facilegreen synthesis techniqueis becoming more and more important, and it has been proposed as a potential substitute for chemical techniques. The current study describes a low-cost, environmentally friendly method for producing tungsten trioxide (WO3) and tantalum (Ta) doped WO3nanoparticles that uses 15 % (w/v) Azadirachta indica (Neem) leaf extract and different concentrations of Ta dopant (1 to 5 %) due to its well-matched ionic radius with WO3. Various techniques FESEM, TEM, EDX, BET, UV-Vis and PL, XRD, and FTIR were used to illustrate the morphological, elemental, optical, structural, and vibrational analysis of the synthesized nanoparticles respectively. Interestingly, the band gap was significantly reduced to 1.88 eV by the addition of a dopant element. For 3 % Ta/WO3, the average particle size was also reduced to 31.6 nm. The synthesized WO3nanoparticles employed in the current study have been used for photocatalytic activitypurposes. Methylene blue (MB), one of the principal water pollutants, was degraded more quickly by the synthesized Ta/WO3nanoparticles when exposed to UV radiation. Among them, 3 % Ta/WO3 gives significantly higher photodegradation 89 % attributed to the Burstein-Moss effect. The significant output of optimized nano-photocatalyst has been observed from the trapping experiment and reusability test. Furthermore, Zeta potential and TOC analysis have been taken to check the stability and mineralization performance. Additionally, the results of the simulation that was carried out using the finite element analysis approach in the RF module of COMSOL Multiphysics 5.3a are quite similar to the experimental findings. This simulation method made it easier for readers to understand the numerous aspects of the photocatalytic process that has been discussed here.

Enhancing drought tolerance in cauliflower (Brassica oleracea var. botrytis) by targeting LFY transcription factor modulation via the ethylene precursor, ACCA: an innovative computational approach.

Background: Brassica oleracea var. botrytis is an annual or biennial herbaceous vegetable plant in the Brassicaceae family notable for its edible blossom head. A lot of effort has gone into finding defense-associated proteins in order to better understand how cauliflower and pathogens interact. Endophytes are organisms that live within the host plant and reproduce. Endophytes are bacteria and fungi that reside in plant tissues and can either help or harm the plant. Several species have aided molecular biologists and plant biotechnologists in various ways. Water is essential for a healthy cauliflower bloom. When the weather is hot, this plant dries up, and nitrogen scarcity can be detrimental to cauliflower growth. Objective: The study sought to discern plant growth promoting (PGP) compounds that can amplify drought resilience and boost productivity in cauliflower. Methods: Investigations were centered on endophytes, microorganisms existing within plant tissues. The dual role of beneficial and detrimental Agrobacterium was scrutinized, particularly emphasizing the ethylene precursor compound, 1-amino-cyclopropane-1-carboxylic acid (ACCA). Results: ACCA possessed salient PGP traits, particularly demonstrating a pronounced enhancement of drought resistance in cauliflower plants. Specifically, during the pivotal marketable curd maturity phase, which necessitates defense against various threats, ACCA showcased a binding energy of -8.74 kcal/mol. Conclusion: ACCA holds a significant promise in agricultural productivity, with its potential to boost drought resistance and cauliflower yield. This could be particularly impactful for regions grappling with high temperatures and possible nitrogen shortages. Future research should explore ACCA's performance under diverse environmental settings and its applicability in other crops.

ADME Study, Molecular Docking, Elucidating the Selectivities and the Mechanism of [4 + 2] Cycloaddition Reaction Between (E)-N ((dimethylamino)methylene)benzothioamide and (S)-3-acryloyl-4-phenyloxazolidin-2-one.

The molecular electron density theory (MEDT) was employed to examine the [4 + 2] cycloaddition reaction between (E)-N-((dimethylamino)methylene)benzothioamide (1) and (S)-3-acryloyl-4-phenyloxazolidin-2-one (2) at the B3LYP/6-311++G(d,p) design level. Parr functions and energy studies clearly show that this reaction is regio- and stereoselective, in perfect agreement with experimental results. By evaluating the chemical mechanism in terms of bond evolution theory (BET) and electron localization function (ELF), which divulges a variety of variations in the electron density along the reaction path, a single-step mechanism with highly asynchronous transition states structures was revealed. Additionally, we conducted a docking study on compounds P1, P2, P3, and P4 in the SARS-CoV-2 main protease (6LU7) in comparison to Nirmatrelvir. Our findings provide confirmation that product P4 may serve as a potent antiviral drug.

Deciphering Multi-target Pharmacological Mechanism of Cucurbita pepo Seeds against Kidney Stones: Network Pharmacology and Molecular Docking Approach.

BACKGROUND: Urolithiasis is a prevalent condition with significant morbidity and economic implications. The economic burden associated with urolithiasis primarily stems from medical expenses. Previous literature suggests that herbal plants, including Cucurbita pepo, have lithotriptic capabilities. C. pepo is an annual, herbaceous, widely grown, and monoecious vegetative plant known for its antioxidants, fibers, and fatty acids. Recent studies on C. pepo seeds have shown therapeutic potential in reducing bladder stones and urodynamic illnesses, like kidney stones. However, the precise molecular and pharmacological mechanisms are unclear. OBJECTIVE: In this research, we employed network pharmacology and molecular docking to examine the active compounds and biological mechanisms of Cucurbita pepo against kidney stones. METHODS: Active constituents were obtained from previous studies and the IMPPAT database, with their targets predicted using Swiss target prediction. Kidney stone-associated genes were collected from DisGeNET and GeneCards. The active constituent-target-pathway network was constructed using Cytoscape, and the target protein-protein interaction network was generated using the STRING database. Gene enrichment analysis of C. pepo core targets was conducted using DAVID. Molecular docking was performed to identify potential kidney stone-fighting agents. RESULTS: The findings revealed that Cucurbita pepo contains 18 active components and has 192 potential gene targets, including AR, EGFR, ESR1, AKT1, MAPK3, SRC, and MTOR. Network analysis demonstrated that C. pepo seeds may prevent kidney stones by influencing disease-related signaling pathways. Molecular docking indicated that key kidney stone targets (mTOR, EGFR, AR, and ESR1) effectively bind with active constituents of C. pepo. CONCLUSION: These findings provide insight into the anti-kidney stone effects of Cucurbita pepo at a molecular level. In conclusion, this study contributes to understanding the potential of Cucurbita pepo in combating kidney stones and lays the foundation for further research.

Differential capacity of phragmites ecotypes in remediation of inorganic contaminants in coastal ecosystems: Implications for climate change.

Remediating inorganic pollutants is an important part of protecting coastal ecosystems, which are especially at risk from the effects of climate change. Different Phragmites karka (Retz) Trin. ex Steud ecotypes were gathered from a variety of environments, and their abilities to remove inorganic contaminants from coastal wetlands were assessed. The goal is to learn how these ecotypes process innovation might help reduce the negative impacts of climate change on coastal environments. The Phragmites karka ecotype E1, found in a coastal environment in Ichkera that was impacted by residential wastewater, has higher biomass production and photosynthetic pigment content than the Phragmites karka ecotypes E2 (Kalsh) and E3 (Gatwala). Osmoprotectant accumulation was similar across ecotypes, suggesting that all were able to successfully adapt to polluted marine environments. The levels of both total soluble sugars and proteins were highest in E2. The amount of glycine betaine (GB) rose across the board, with the highest levels being found in the E3 ecotype. The study also demonstrated that differing coastal habitats significantly influenced the antioxidant activity of all ecotypes, with E1 displaying the lowest superoxide dismutase (SOD) activity, while E2 exhibited the lowest peroxidase (POD) and catalase (CAT) activities. Significant morphological changes were evident in E3, such as an expansion of the phloem, vascular bundle, and metaxylem cell areas. When compared to the E3 ecotype, the E1 and E2 ecotypes showed striking improvements across the board in leaf anatomy. Mechanistic links between architectural and physio-biochemical alterations are crucial to the ecological survival of different ecotypes of Phragmites karka in coastal environments affected by climate change. Their robustness and capacity to reduce pollution can help coastal ecosystems endure in the face of persistent climate change.

Extracellular synthesis of silver nanoparticle using yeast extracts: antibacterial and seed priming applicationss.

The evolution and rapid spread of multidrug-resistant (MDR) bacterial pathogens have become a major concern for human health and demand the development of alternative antimicrobial agents to combat this emergent threat. Conventional intracellular methods for producing metal nanoparticles (NPs) using whole-cell microorganisms have limitations, including binding of NPs to cellular components, potential product loss, and environmental contamination. In contrast, this study introduces a green, extracellular, and sustainable methodology for the bio-materialization of silver NPs (AgNPs) using renewable resource cell-free yeast extract. These extracts serve as a sustainable, biogenic route for both reducing the metal precursor and stabilizing the surface of AgNPs. This method offers several advantages such as cost-effectiveness, environment-friendliness, ease of synthesis, and scalability. HR-TEM imaging of the biosynthesized AgNPs revealed an isotropic growth route, resulting in an average size of about ~ 18 nm and shapes ranging from spherical to oval. Further characterization by FTIR and XPS results revealed various functional groups, including carboxyl, hydroxyl, and amide contribute to enhanced colloidal stability. AgNPs exhibited potent antibacterial activity against tested MDR strains, showing particularly high efficacy against Gram-negative bacteria. These findings suggest their potential role in developing alternative treatments to address the growing threat of antimicrobial resistance. Additionally, seed priming experiments demonstrated that pre-sowing treatment with AgNPs improves both the germination rate and survival of Sorghum jowar and Zea mays seedlings. KEY POINTS: •Yeast extract enables efficient, cost-effective, and eco-friendly AgNP synthesis. •Biosynthesized AgNPs showed strong antibacterial activity against MDR bacteria. •AgNPs boost seed germination and protect against seed-borne diseases.

Alginic acid-functionalized silver nanoparticles: A rapid monitoring tool for detecting the technology-critical element tellurium.

The increasing global demand for tellurium, driven by its critical role in alloys, photovoltaic devices, and electronics, has raised concerns about its environmental pollution and neurotoxicity. In response, the potential of alginic acid (AA), a renewable, low-cost, and sustainable biopolymer, was explored for the biosynthesis of ultra-small silver nanoparticles (AgNPs) and their application in the detection of tellurium (Te(IV)). The effect of key synthesis parameters on desired physicochemical properties and yield of AgNPs was established to ensure high specificity and sensitivity towards Te(IV). The purified AgNPs with AA surface ligands were utilized to demonstrate a ratiometric absorbance sensor that exhibits excellent linearity and nanomolar-level affinity. This approach achieved a high correlation coefficient of ∼ 0.982, with a low detection limit of about 22 nM. Further investigations into the effect of pH, ionic strength, and organic molecules were conducted to elucidate detection performance and molecular understanding. The detection mechanism relies on the coordination between Te(IV) ions and the carboxylate groups of AA, which initiates aggregation-induced plasmon coupling in adjacent AgNPs. The capability of this analytical method to monitor Te(IV) in real-world water samples features its rapidity, user-friendliness, and suitability for point-of-care monitoring, making it a promising alternative to more complex techniques.

Regulation of fungal communities during pig manure composting.

The role of protein shell (PS) amendment in altering the fungal community during pig manure (PM) composting was investigated. Six different dosages of PS based on the dry weight of PM (0 %, 2.5 %, 5 %, 7.5 %, 10 %, and 12 %; T1-T6, respectively) were mixed with wheat straw to make the initial feedstock and composted for 42 days. The results showed that Ascomycota, Basidiomycota, and Giomeromycota were the most abundant phyla in all treatments. However, the relative abundance of Giomeromycota was the highest in the control treatment, although a substantially greater population was observed in all treatments. Genus abundance declined steadily from T1 to T6; however, T4 and T6 had smaller populations. Correlation analysis also suggested that T6 amendment increased the overall fungal dynamics and organic matter degradation. Thus, T6 was more efficient to enhance the overall fungal population and dynamics with considerable network connections among all the analyzed parameters.

Date seed waste derived nanocatalyst and its application in production of hydrolytic enzyme, fermentative sugars and biohydrogen.

Biofuel production from cellulosic biomass is a promising approach; however, the cost-intensive utilization of cellulolytic enzymes is a major roadblock to economic production. This study reports the preparation of a nanocatalyst using date seed and evaluates the impact of nanocatalysts on cellulolytic enzyme production using solid-state fermentation of date pulp waste through bacterial co-cultivation. Under optimized conditions, 30 IU/gds filter paper activity is produced in the presence of 2 mg of nanocatalyst. Cellulase showed thermal stability at 50 °C and pH 7 up to 10 h in the presence of nanocatalyst, and it produced 32.31 g/L glucose through the hydrolysis of acidic-pretreated date seeds in 24 h. Subsequently, 1788 mL/L of cumulative H2 in 24 h through cocultured dark fermentation could be produced. The approach presented in this study can be effective for multiple value additions, including nanocatalyst preparation, cellulase enzyme, and biohydrogen production.

Assessing phytotoxicity and cyto-genotoxicity of two insecticides using a battery of in-vitro biological assays.

Intensive use of chemical pesticides in agriculture poses environmental risks and may have negative impacts on agricultural productivity. The potential phytotoxicity of two chemical pesticides, chlorpyrifos (CPS) and fensulfothion (FSN), were evaluated using Cicer arietinum and Allium cepa as model crops. Different concentrations (0-100 µgmL-1) of both CPS and FSN decreased germination and biological attributes of C. arietinum. High pesticide doses significantly (p ≤ 0.05) caused membrane damage by producing thiobarbituric acid reactive substances (TBARS) and increasing proline (Pro) content. Pesticides elevated ROS levels and substantially increased the superoxide anions and H2O2 concentrations, thus aggravating cell injury. Plants exposed to high pesticide dosages displayed significantly higher antioxidant levels to combat pesticide-induced oxidative stress. Ascorbate peroxidase (APX), guaiacol peroxidase (GPX), catalase (CAT), peroxidase (POD), and superoxide dismutase (SOD) increased by 48%, 93%, 71%, 52% and 94%, respectively, in C. arietinum roots exposed to 100 µgFSNmL-1. Under CLSM, pesticide-exposed C. arietinum and 2',7'-dichlorodihydrofluorescein diacetate (2'7'-DCF) and 3,3'-diaminobenzidine stained roots exhibited increased ROS production in a concentration-dependent manner. Additionally, enhanced Rhodamine 123 (Rhd 123) and Evan's blue fluorescence in roots, as well as changes in mitochondrial membrane potential (ΔΨm) and cellular apoptosis, were both associated with high pesticide dose. Allium cepa chromosomal aberration (CAs) assay showed a clear reduction in mitotic index (MI) and numerous chromosomal anomalies in root meristematic cells. Additionally, a-dose-dependent increase in DNA damage in root meristematic cells of A. cepa and conversion of the super-coiled form of DNA to open circular in pBR322 plasmid revealed the genotoxic potential of pesticides. The application of CPS and FSN suggests phytotoxic and cyto-genotoxic effects that emphasize the importance of careful monitoring of current pesticide level in soil before application and addition at optimal levels to soil-plant system. It is appropriate to prepare both target-specific and slow-release agrochemical formulations for crop protection with concurrent safeguarding of agroecosystems.

2-Oxoquinoline-based-thiosemicarbazones as multitargeting neurotherapeutics against Alzheimer's disease: In vitro and in silico studies of MAO and ChE inhibitors.

Alzheimer's disease (AD) presents a multifactorial neurological disorder with multiple enzyme involvement in its onset. Conventional monotherapies fall short in providing long-term relief, necessitating the exploration of alternative multitargeting approaches to address the complexity of AD. Therefore, the design, synthesis, and in vitro and in silico evaluation of 2-oxoquinoline-based thiosemicarbazones 9a-r as multipotent analogs, able to simultaneously inhibit the cholinesterase (ChE) and monoamine oxidase (MAO) enzymes for the potential treatment of AD, are reported. In the in vitro experimental evaluation of MAO and ChE inhibition, all tested compounds demonstrated remarkable potency exhibiting nonselective inhibition of both MAO-A and MAO-B, and selective inhibition of acetylcholinesterase (AChE) over butyrylcholinesterase (BChE), with 9d, 9j, and 9m evolving as lead compounds for MAO-A, MAO-B, and AChE, displaying IC50 values of 0.35 ± 0.92, 0.50 ± 0.02, and 0.25 ± 0.13 µM, respectively. Moreover, the kinetic studies revealed that all tested compounds inhibited all three enzymes through a competitive mode of inhibition. Furthermore, the molecular docking studies of the most active compounds revealed several crucial interactions, particularly hydrogen bonding interactions. These interactions were observed between the nitrogen and sulfur atoms of thiosemicarbazone and the nitrogen and oxygen atoms of the quinoline ring with various amino acids, suggesting the strong interactions of these compounds with the enzymes.

Exploring carbon conversion and balance with magnetite-amended during pig manure composting.

This study was designed to explore the magnetite in maturation and humification during pig manure (PM) and wolfberry branch fillings (BF) composting. Different proportions of magnetite (T1, 0%; T2, 2.5%; T3, 5%; T4, 7.5%;) were blended with PM for 50 days of composting. The findings indicated magnetite amendment has no influence on the maturity, and the 5% ratio significantly promoted humic acid (HA) formation and fulvic acid (FA) decomposition compared to other treatments. Compared to T1, magnetite addition significantly increased CO2 and CH4 emissions by 106.39%-191.69% and 6.88-13.72 times. The further analysis suggested that magnetite improved Ruminofilibacter activity were significantly positively associated with HA, and C emissions. The further PICRUSt 2 analysis showed membrane transport may enhance environmental information processing by magnetite. Overall, these results demonstrated higher organic matter (OM) degradation and HA formation with an additional increase in microbial activity highlighted advantages of using magnetite during PM composting.

Exploring the efficacy of 1-amino-cyclopropane-1-carboxylic acid (ACCA) as a natural compound in strengthening maize resistance against biotic and abiotic stressors: an empirical computational study.

Objective: This study aims to understand plant-bacteria interactions that enhance plant resistance to environmental stressors, with a focus on maize (Zea mays L.) and its vulnerability to various pathogenic organisms. We examine the potential of 1-amino-cyclopropane-1-carboxylic acid (ACCA) as a compound to boost maize's resilience against stressors and pathogens. Background: With the growing global population and increased food demand, the study of endophytes, comprising bacteria and fungi, becomes crucial. They reside within plant tissues, affecting their hosts either beneficially or detrimentally. Agrobacteria are of specific interest due to their potential to contribute to developing strategies for plant resistance enhancement. Methods: We conducted exhaustive research on the defense-related proteins and mechanisms involved in maize-pathogen interactions. The efficacy of ACCA as a natural-compound that could enhance maize's resistance was examined. Results: Our research indicates that ACCA, having a binding energy of -9.98 kcal/mol, successfully strengthens maize resistance against pathogenic assaults and drought stress. It plays a crucial protective role in maize plants as they mature, outperforming other ligands in its effectiveness to improve productivity and increase yield. Conclusion: Applying ACCA to maize plants has considerable potential in enhancing their resilience and tolerance to stress, proving to be an effective strategy to boost crop yield and productivity. This could help address the increasing global food demand. However, more research is needed to optimize ACCA application methods and to gain a comprehensive understanding of its long-term effects on maize cultivations and the environment.

The efficient solution to decline the greenhouses emission and enrich the bacterial community during pig manure composting: Regulating the particle size of cornstalk.

In present study, four lengths of chopped cornstalks were amended with pig manure respectively for 100 days aerobic fermentation, which aimed to evaluate the impact of different length of agricultural solid wastes on gaseous emission and dominating bacterial community succession and connection. The result revealed that the maximum ammonia volatilization was observed in 5 cm of straw samples attributed to the prominent mineralization, which was opposite to the emission of CH4 and N2O. As for global warming potential, the minimum value was detected in 5 cm of straw samples, which decreased by 5.03-24.75% compared with other samples. Additionally, the strongest correlation and complexity of bacterial community could be detected in 5 cm of straw treatment, representing the most vigorous bacterial metabolic ability could be recorded by optimizing the microbial habitat. Therefore, in order to decline the greenhouse effect in livestock manure composting, the 5 cm of corn straw was recommended.

Explaining the selectivities and the mechanism of [3+2] cycloloaddition reaction between isoalantolactone and diazocyclopropane.

CONTEXT: [3+2] cycloaddition processes between isoalantolactone (ISALL) and diazocyclopropane (DCYP), have been surveyed exercising the MEDT, reactivity indices, reactions, and activation energies, are computed. In an investigation of conceptual DFT indices, DCYP behaves as a nucleophile in this reaction, whereas ISALL acts as an electrophile. This cyclization is stereo-, chemo-, and regiospecific, as demonstrated by the activation and reaction energies, in clear agreement with the experiment's results. The mechanism for this [3+2] cycloaddition is occurring in two steps, according to ELF analysis. METHODS: For the purposes of this investigation, all computations were performed using the Gaussian 09 program. The optimization was completed using Berny's computational gradient optimization approach with the basis set 6-311G(d,p) and wB97XD functional. Frequency computations were utilized to characterize and locate stationary points where the transition phases have just one imaginary frequency and all frequencies for the reactants and products are positive. After evaluating the effect of dichloromethane (DCM) as a reaction solvent, the stationary point optimization was updated using the polarizable continuum model (PCM) developed by the Tomasi team. The electron localization function (ELF) has been examined within the context of topological investigations using Multiwfn software with a 0.05 grid step.

Influence of biochar on succession of fungal communities during food waste composting.

This study aims to examine the effects of biochar on fungal dynamics during food waste composting. The different dosage of wheat straw biochar from 0 to 15% (0%, 2.5%, 5%, 7.5%, 10%, and 15%) were used as an additive to composting and examined for 42 days. The results showed that Ascomycota (94.64%) and Basidiomycota (5.36%) were the most dominant phyla. The most common fungal genera were Kluyveromyces (3.76%), Candida (5.34%), Trichoderma (2.30%), Fusarium (0.46%), Mycothermus-thermophilus (5.67%), Trametes (0.46%), and Trichosporon (3.38%). The average number of operational taxonomic units were 469, with the greatest abundance seen in the 7.5% and 10% treatments. Redundancy analysis revealed that different concentrations of biochar applied treatments have significantly distinct fungal communities. Additionally, correlation analyses of fungal interactions with environmental elements, performed through a heatmap, also indicate a distinct difference among the treatments. The study clearly demonstrates that 15% of biochar has a positive impact on fungal diversity and improves the food waste composting.

Evaluation of fungal dynamics during sheep manure composting employing peach shell biochar.

In this study, explored the influence of different proportion (0%, 2.5%, 5%, 7.5%, and 10%) peach shell biochar (PSB) with microbial agents (EM) on the carbon transformation, humification process and fungal community dynamics during sheep manure (SM) composting. And no additives were used as control. The results manifested that the CO2 and CH4 emissions were effectively reduced 8.23%∼13.10% and 17.92%∼33.71%. The degradation rate of fulvic acid increased by 17.12%∼23.08% and the humic acid contents were enhanced by 27.27%∼33.97% so that accelerated the composting. Besides, the dominant fungal phylum was Ascomycota (31.43%∼52.54%), Basidiomycota (3.12%∼13.85%), Mucoromycota (0.40%∼7.61%) and Mortierellomycota (0.97%∼2.39%). Pearson correlation analysis and network indicated that there were different correlations between physicochemical indexes and fungal community under different additive concentrations. In brief, the two modifiers application promoted the SM degradation and affected the fungal community structure.

Microbial dynamics and nitrogen retention during sheep manure composting employing peach shell biochar.

In this study, the effects of peach shell biochar (PSB) and microbial agent (EM) amendment on nitrogen conservation and bacterial dynamics during sheep manure (SM) composting were examined. Six treatments were performed including T1 (control with no addition), T2 (EM), T3 (EM + 2.5 %PSB), T4 (EM + 5 %PSB), T5 (EM + 7.5 %PSB), and T6 (EM + 10 %PSB). The results showed that the additives amendment reduced NH3 emissions by 6.12%∼32.88% and N2O emissions by 10.96%∼19.76%, while increased total Kjeldahl nitrogen (TKN) content by 8.15-9.13 g/kg. Meanwhile, Firmicutes were the dominant flora in the thermophilic stages, while Proteobacteria, Actinobacteriota, and Bacteroidota were the dominant flora in the maturation stages. The abundance of Bacteroidota and Actinobacteriota were increased by 17.49%∼32.51% and 2.31%∼12.60%, respectively, which can accelerate the degradable organic materials decomposition. Additionally, redundancy analysis showed that Proteobacteria, Actinobacteriota, and Bacteroidota were positively correlated with NO3--N, TKN, and N2O, but a negative correlation with NH3 and NH4+-N. Finally, results confirmed that (EM + 10 %PSB) additives were more effective to reduce nitrogen loss and improve bacterial dynamics.

Effects of magnesite on nitrogen conversion and bacterial community during pig manure composting.

The objective of this research was to elucidate the effect of varying proportions of magnesite (MS) addition - 0% (T1), 2.5% (T2), 5% (T3), 7.5% (T4), and 10% (T5) - on nitrogen transformation and bacterial community dynamics during pig manure composting. In comparison to T1 (control), MS treatments amplified the abundance of Firmicutes, Actinobacteriota, and Halanaerobiaeota, bolstered the metabolic functionality of associated microorganisms, and enhanced the nitrogenous substance metabolic pathway. A complementary effect in core bacillus species played a key role in nitrogen preservation. Compared to T1, 10% MS demonstrated the most substantial influence on composting because Total Kjeldahl Nitrogen increased by 58.31% and NH3 emission decreased by 41.52%. In conclusion, 10% MS appears to be optimal for pig manure composting, as it can augment microbial abundance and mitigate nitrogen loss. This study offers a more ecologically sound and economically viable method for curtailing nitrogen loss during composting.