Step-feeding food waste fermentation liquid as supplementary carbon source for low C/N municipal wastewater treatment: Bench scale performance and response of microbial community.

Municipal wastewater treatment often lacks carbon source, while carbon-rich organics in food waste are deficiently utilized. In this study, the food waste fermentation liquid (FWFL) was step-fed into a bench-scale step-feed three-stage anoxic/aerobic system (SFTS-A/O), to investigate its performance in nutrients removal and the response of microbial community as a supplementary carbon source. The results showed that the total nitrogen (TN) removal rate increased by 21.8-109.3% after step-feeding FWFL. However, the biomass of the SFTS-A/O system was increased by 14.6% and 11.9% in the two phases of the experiment, respectively. Proteobacteria was found to be the dominant functional phyla induced by FWFL, and the increase of its abundance attributed to the enrichment of denitrifying bacteria and carbohydrate-metabolizing bacteria was responsible for the biomass increase. Azospira belonged to Proteobacteria phylum was the dominant denitrifying genera when step-fed with FWFL, its abundance was increased from 2.7% in series 1 (S1) to 18.6% in series 2 (S2) and became the keystone species in the microbial networks. Metagenomics analysis revealed that step-feeding FWFL enhanced the abundance of denitrification and carbohydrates-metabolism genes, which were encode mainly by Proteobacteria. This study constitutes a key step towards the application of FWFL as a supplementary carbon source for low C/N municipal wastewater treatment.

Biomanipulation as a strategy for minimizing ecological risks in river supplied with reclaimed water.

Reclaimed water is an effective method for addressing water pollution and shortages. However, its use may contribute to the collapse of receiving water (algal blooms and eutrophication) owing to its unique characteristics. A three-year biomanipulation project was conducted in Beijing to investigate the structural changes, stability, and potential risks to aquatic ecosystems associated with the reuse of reclaimed water in rivers. During the biomanipulation, the proportion of Cyanophyta in the community structure of phytoplankton density in river supplied with reclaimed water decreased, and the community composition shifted from Cyanophyta and Chlorophyta to Chlorophyta and Bacillariophyta. The biomanipulation project increased the number of zoobenthos and fish species and significantly increased fish density. Despite the significant difference in aquatic organisms community structure, diversity index and community stability of aquatic organisms remained stable during the biomanipulation. Our study provides a strategy for minimizing the hazards of reclaimed water through biomanipulation by reconstructing the community structure of reclaimed water, thereby making it safe for large-scale reuse in rivers.

Long-period D-fiber grating based robust and efficient bidirectional coupler for whispering gallery mode excitation.

A robust and efficient bidirectional coupler for whispering gallery mode (WGM) excitation based on a long-period grating (LPG) inscribed in D-fiber is theoretically and experimentally demonstrated. The LPG coupling the fundamental core mode to the forward propagating cladding modes according to the phase-matching condition not only enhances the evanescent field of the fiber but also selectively excites the WGM in a wavelength band of interest. Experimental results show that a maximum resonance contrast as high as 10.5 dB and a quality factor (Q-factor) on the order of 104 can be achieved in an LPG coupled spherical silica WGM resonator with a diameter of 242 µm, where the LPG with a pitch of 680 µm is fabricated by arc-discharging in a side-polished D-fiber with a maximum polishing depth of 56 µm. In addition to high robustness and efficiency, such an LPG-based WGM coupler also demonstrates bidirectionality, i.e., it is independent of the injection direction of the input light, which provides a reliable and flexible fiber coupler for the WGM resonator based practical applications.

Microbial mechanisms of refractory organics degradation in old landfill leachate by a combined process of UASB-A/O-USSB.

A combined process of anaerobic digestion (UASB), shortcut nitrification-denitrification (A/O), and semi-anoxic co-metabolism (operated by an up-flow semi-anoxic sludge bed; USSB) was constructed for the treatment of old landfill leachate (>10 years). The performance and mechanism of refractory organics degradation by the combined process (UASB-A/O-USSB) were investigated. The results showed that the semi-anoxic co-metabolism contributes 57 % of the totally degraded refractory organics. Specific microorganisms and their corresponding metabolic functions drive the degradation of refractory organics in each unit of the UASB-A/O-USSB process. In detail, organics with simple molecular structures were preferentially degraded by anaerobic digestion and shortcut denitrification, whereas those with complex structures were subsequently degraded in the oxic tanks and USSB reactor by shortcut nitrification and semi-anoxic co-metabolism. The structural equation model showed that the combined process of shortcut nitrification and semi-anoxic co-metabolism had a better effect on the degradation of recalcitrant organics than the single process. These findings provide information on how refractory organics are metabolically degraded in a combined process.

Control of polarization switching in a VCSEL via resonant feedback from a whispering-gallery-mode cavity.

We report a method for flexibly switching the dominant polarization of a vertical-cavity surface-emitting laser (VCSEL) by introducing polarization-resolved resonant optical feedback from a whispering-gallery-mode (WGM) cavity to the lasing cavity. Switching between the originally dominant mode and a side mode is experimentally demonstrated under different bias currents once one of them is locked to the resonance mode of the WGM cavity. In addition to a controllable polarization state, the reported VCSEL also demonstrates a linewidth as narrow as tens of kilohertz, which is highly desirable for many applications, including high-speed data communication, light detection and ranging (lidar), and absorption spectroscopy.

Simultaneous self-injection locking of two VCSELs to a single whispering-gallery-mode microcavity.

Simultaneous self-injection locking of two vertical-cavity surface-emitting lasers (VCSELs) to a single whispering-gallery-mode (WGM) microcavity is experimentally demonstrated. The linewidths of the two VCSELs are compressed from 3.5 MHz and 5 MHz to 20.9 kHz and 24.1 kHz, which is on the same order of magnitude as that of locking each VCSEL to the microcavity separately. Moreover, the frequency noises of the two simultaneously locked VCSELs are suppressed by more than 60 dB below the offset frequency of 100 kHz compared to that of the free-running VCSELs. The method demonstrated here might be used in the multi-wavelength laser array with low phase and frequency noises, especially the VCSELs with the unique architecture of a two-dimensional array.