[Observation on the distribution of nerve fibers and neural cells morphology in Aspidogaster conchiola].

OBJECTIVE: To understand the distribution of nerve fibers and the types of neural cells in Aspidogaster conchiola. METHODS: Whole worms were subjected to silver staining, histochemical staining and hematoxylin-eosin (HE) staining, and the nervous systems of the worms were observed. RESULTS: There were 3 types of neural cells in the worm head near the cerebral ganglion, including unipolar, bipolar and multipolar neurons, which were divided into 7 types according to the morphology. There was a nerve network on the surface of pharynx and intestinal tract, as well as the reproductive organ, including testis, ovary, lower uterus and penis sac. The nerve network was consisted of circular and longitudinal nerve fibers, and the structure of the nerve network around the mouth was similar to central nerve. CONCLUSIONS: The structure of the A. conchiola central nervous system is very complicated, and the neural networks may be associated with the physiologic activity of the worm. Different neural cells may have diverse functions.

[microRNA-98 and microRNA-143 in nasal and paranasal sinus carcinomas and its clinical significance].

Objective:To investigate the role and clinical significance of the expression of microRNA-98(miRNA-98)and microRNA-143(miRNA-143)in development and progression of nasal and paranasal sinus carcinomas.Method:The expression miRNA-98 and miRNA-143 was detected by Real time PCR metheod in the 53 nasal and paranasal sinus carcinomas and 50 nasal polyp tissues and 20 cases of normal muscosa.The expression of miRNA-98 was analyzed in nasal and paranasal sinus carcinomas with different clinicopathogical parameters.Result:The expression of miRNA-98 in nasal and paranasal sinus carcinomas was obvious higher than that in inflammation of the nasal polyp tissues and normal muscosa,which had statistical significant difference(P<0.05).There was a positive correlation between miRNA-98 expression and TNM staging ,lymph node metastasis;but not pathological grade.The expression of miRNA-143 in nasal and paranasal sinus carcinomas was obvious lower than that in nasal polyp tissues and normal muscosa,which had statistical significant difference(P<0.05).Conculusion:miRNA-98 highly expressed in nasal and paranasal sinus carcinomas,miRNA-143 lowly expressed in nasal and paranasal sinus carcinomas.

Phosalone applied to cotton (Gossypium hirsutum L.): its deposition and persistence on foliages, soils and final residues in seeds.

Phosalone, O,O-diethyl-S-(6-chloro-1,3-benzoxazol-2(3H)-onyl)methyl phosphorodithioate, was field-applied by ground equipment to cotton (Gossypium hirsutum L.) at the rates of 1050 and 2100 g a.i./ha, respectively, to determine its dissipation on leaves and soils and the residues in seeds at harvest. The insecticide concentrations on cotton leaves and soils were measured periodically for 14 days following its application. It was found that the half lives of the insecticide on cotton leaves at the dosages of 1050 and 2100 g a.i./ha were 6.8 and 6.3 days, respectively. And the half lives on soils for the 2 dosages were 7 and 5.8 days, respectively. The residues remaining in soils at harvest time were 0.072 and 0.121 mg/kg 14 days post-application and the residues in cotton seeds were relatively low (less than 0.02-0.12 mg/kg).

Improved cleanup for gas chromatographic determination of propiconazole residues in soil, wheat grain, straw, and leaves.

A simple and sensitive method is described for determination of propiconazole, a new type of broad-spectrum systemic fungicide, in soil, wheat grain, straw, and leaves. Pesticide residues in or on grain and green plant materials are extracted with methanol (or a mixture of methanol and water (4 + 1), for soil), partitioned into methylene chloride, and cleaned up on an alumina column for grain and soil or an activated charcoal column for green plant materials. The amount of residue is quantitatively measured by gas chromatography using an alkali flame ionization detector in the nitrogen-sensitive mode. Recoveries from soil, grain, and green plant materials fortified at 0.1-5 mg/kg are better than 80%. The practical detection limits of this method are 0.01 mg/kg in grain and soil and 0.02 mg/kg in green plant materials.