The Role of MicroRNA-206 in the Regulation of Diabetic Wound Healing via Hypoxia-Inducible Factor 1-Alpha.

Successful wound healing in diabetic patients is hindered by dysregulated miRNA expression. This study aimed to investigate the abnormal expression of miRNAs in diabetic wound healing and the potential therapeutic role of modulating the miR-206/HIF-1α pathway. MicroRNA assays were used to identify differentially expressed miRNAs in diabetic wound sites and adjacent areas. In vitro models and a rat diabetic model were established to evaluate the effects of miR-206 on HIF-1α regulation and wound healing. The study revealed differential expression of miR-206 in diabetic wound tissues, its interaction with HIF-1α, and the inhibitory effect of miR-206 on cell growth under high glucose conditions. Modulating the miR-206/HIF-1α pathway using miR-206 antagomir promoted HIF-1α, CD34, and VEGF expression, ultimately enhancing diabetic wound healing.

Discovery and structure characteristics of the intermediate-state conformation of poly(9,9-dioctylfluorene) (PFO) in the dynamic process of conformation transformation and its effects on carrier mobility.

Good solution processability is a prerequisite for fabricating polymer optoelectronic devices. In this research, a new PFO chain conformation called "intermediate-state conformation" was found through UV-vis absorption spectroscopy, photoluminescence spectroscopy (PL) and Raman spectroscopy in the transition process of α conformation towards ß conformation. The intermediate-state conformation not only remedies the defect of film-forming caused by large aggregation of ß-conformation but also gains an equivalent carrier mobility similar to that of ß conformation. Simultaneously, it was found that the film with the intermediate-state conformation had a smooth surface morphology compared to the film with ß-conformation, which indicates that the intermediate-state conformation has good solution processability; thus, it is more suitable for the fabrication of photoelectric films with high carrier mobility. The results of high-resolution transmission electron microscopy (HR-TEM) measurements showed that there were obvious lattice fringes in the films with the intermediate-state conformation and ß conformation; this reveals that the intermediate-state conformation has a more planar conformation with extended conjugation length than the ß conformation, which is very beneficial to enhance carrier mobility. The research significantly reveals the dynamic evolution of polymer structures based on conjugated polymer physics. The conclusions enrich the understanding of the structure evolution and dynamic process of conjugated polymers and present broad application prospects for photoelectric and other functional devices due to the good film-forming properties of the intermediate-state conformation.

A transformation process and mechanism between the α-conformation and ß-conformation of conjugated polymer PFO in precursor solution.

In this work, the solvent field and temperature are used to explore the mutual transformation dynamic process and mechanism between the α-conformation and ß-conformation in poly(9,9-dioctylfluorene) (PFO) precursor solution. The conformational transformation of the PFO chain is researched by UV-vis absorption spectra and the proportions of the ß-conformation are quantitatively calculated. The corresponding variation trend of the aggregation structure is researched using a static and dynamic light scattering (SLS/DLS) method. It is found that the mutual transformation processes between the α-conformation and ß-conformation are reversible in essence. Especially in the transformation processes, the complicated relationship between the ß-conformation and the aggregation structure is understood, that is the aggregation structure promotes formation of the ß-conformation under solvent field, then the conformational transformation of the ß-conformation promotes the dissociation of the aggregation structure under temperature. The above results give an insight into the ß-conformation and the aggregation structure of PFO in theory. Furthermore, under the temperature, we find that both two transformation steps have good linear correlations, which indicates that using temperature can be considered as a good method to accurately control the proportion of ß-conformation in actual applications, and it will help us to get the desired proportion of the ß-conformation in PFO precursor solution so as to make the charge carrier mobility of optoelectronic films increased and device performance better.