RESUMO
Aujeszky disease (AD) or pseudorabies is a viral disease of domestic and wild animals caused by the Suid alphaherpesvirus 1. In wild boar infection usually undergo latent phase but under certain conditions reactivation of the virus can result in a disease. Seroprevalence in wild boars ranges from 0.8 to 100%, and is among other influenced by region, type of management, age and sex of the studied animals. In this study we analyzed blood, lungs, olfactory bulbs and spleen from 222 free-living wild boars from different localities in Croatia and compared results obtained by ELISA with PCR, sex, age and locality. Total seroprevalence was 33.78%, ranging from 25.26% in males to 40.15% in females (p = 0.0346; χ2 = 4.47). According to the age categories prevalence was 10% in offspring, 27.53% in subadults, and 66.75% in adults. Seroprevalence in adult males (66.66%) and females (65.30%) was almost identical. In males, significantly lower seroprevalence was detected in offspring compared to subadults (χ2 = 4.07, p < 0.05) and adults (χ2 = 31.04; p < 0.05), and in subadults compared to adults (χ2 = 15.13; p < 0.0001). Among females, adults had a significantly higher prevalence compared to offspring (χ2 = 19.27; p < 0.0001) and subadults (χ2 = 8.62; p < 0.01). Analysis between counties revealed Sisacko-moslavacka county as a hot-spot for AD. None of the samples was positive for ADV antigens. The observed trend in prevalence points to the fact that the main transmission occurs during one part of the year (most probably the mating season). Also, triggers for virus reactivation might be more complex than previously thought, since none of our samples, collected during the mating and hunting season, was PCR positive. Finally, we can conclude that adult males represent the main transmission link between different wild boar groups.