Your browser doesn't support javascript.
loading
Mostrar: 20 | 50 | 100
Resultados 1 - 1 de 1
Filtrar
Mais filtros

Base de dados
Ano de publicação
Tipo de documento
Intervalo de ano de publicação
1.
Sci Rep ; 8(1): 10952, 2018 Jul 19.
Artigo em Inglês | MEDLINE | ID: mdl-30026575

RESUMO

Rapid evaporative ionisation mass spectrometry (REIMS) is a novel technique for the real-time analysis of biological material. It works by conducting an electrical current through a sample, causing it to rapidly heat and evaporate, with the analyte containing vapour channelled to a mass spectrometer. It was used to characterise the metabolome of 45 Pseudomonas aeruginosa (P. aeruginosa) isolates from cystic fibrosis (CF) patients and compared to 80 non-CF P. aeruginosa. Phospholipids gave the highest signal intensity; 17 rhamnolipids and 18 quorum sensing molecules were detected, demonstrating that REIMS has potential for the study of virulence-related metabolites. P. aeruginosa isolates obtained from respiratory samples showed a higher diversity, which was attributed to the chronic nature of most respiratory infections. The analytical sensitivity of REIMS allowed the detection of a metabolome that could be used to classify individual P. aeruginosa isolates after repeated culturing with 81% accuracy, and an average 83% concordance with multilocus sequence typing. This study underpins the capacities of REIMS as a tool with clinical applications, such as metabolic phenotyping of the important CF pathogen P. aeruginosa, and highlights the potential of metabolic fingerprinting for fine scale characterisation at a sub-species level.


Assuntos
Fibrose Cística/microbiologia , Metabolômica/métodos , Infecções por Pseudomonas/microbiologia , Pseudomonas aeruginosa/classificação , Técnicas de Tipagem Bacteriana , Humanos , Tipagem de Sequências Multilocus , Fenótipo , Pseudomonas aeruginosa/isolamento & purificação , Pseudomonas aeruginosa/metabolismo , Pseudomonas aeruginosa/patogenicidade , Percepção de Quorum , Sensibilidade e Especificidade , Espectrometria de Massas por Ionização por Electrospray , Virulência
SELEÇÃO DE REFERÊNCIAS
DETALHE DA PESQUISA