Nutrition and Health in Arab Adolescents (NaHAR): Study protocol for the determination of ethnic-specific body fat and anthropometric cut-offs to identify metabolic syndrome.

The prevalence of adolescent obesity in the Middle-East is considered among the highest in the world. Obesity in adolescents is associated with several cardiometabolic abnormalities, the constellation of which is referred to as the metabolic syndrome (MetS). This multi-country cross-sectional study aims to determine the optimal cut-off values for body fat (BF); body mass index (BMI) z-score; waist circumference (WC) percentile, and mid-upper arm circumference (MUAC) for the prediction of MetS among adolescents from Kingdom of Saudi-Arabia (KSA), Kuwait, Jordan, Lebanon and Syria. A secondary objective is to examine the validity of Bioelectrical Impendence Vector Analysis (BIVA) in estimating BF against the deuterium dilution technique (DDL). In each country, a sample of 210 adolescents will be recruited. Data collection will include demographics, socioeconomic, lifestyle and dietary data using a multi-component questionnaire; anthropometric measurements will be obtained and body composition will be assessed using the DDL and BIVA; blood pressure and biochemical assessment will be performed for the identification of the MetS. Receiver operating characteristic analyses will be undertaken to determine optimal cut-off values of BMI, WC, MUAC and BF in identifying those with MetS. Odds ratios (OR) and their respective 95% confidence interval (CI) for the association of the anthropometric measurements with MetS will be computed based on multiple logistic regression analysis models. The Bland and Altman approach will be adopted to compare BIVA against the reference DDL method for the determination of body composition parameters. This study responds to the need for ethnic-specific anthropometric cut-offs for the identification of excess adiposity and associated cardiometabolic risks in the adolescent population. The adoption of the generated cut-offs may assist policy makers, public health professionals and clinical practitioners in providing ethnic-specific preventive and curative strategies tailored to adolescents in the region.

Impact of Ramadan Fasting on Cardiometabolic and Inflammatory Biomarkers among Saudi Adults with Diabetes.

BACKGROUND AND AIMS: Diabetes is prevalent in Saudi Arabia. As dietary concerns are central in patients with diabetes, the impact of fasting on health during Ramadan is potentially significant. Decreased meal frequency and changes in food type and amount affect body weight and metabolic profiles. This study aimed to investigate the association between Ramadan fasting and anthropometric measurements and cardiometabolic and inflammatory biomarkers in Saudi adults with diabetes. METHODS: Sixty-eight Saudi adults with diabetes, admitted at the University Diabetes Centre in Riyadh, Saudi Arabia, were enrolled in this study. Participants were evaluated at the following time points: six weeks before Ramadan (pre-fasting) and at least 15 days after the initiation of Ramadan (peri-fasting). Anthropometric measurements and cardiometabolic and inflammatory biomarker levels were assessed during both visits. RESULTS: After accounting for confounding factors, Ramadan fasting was found to be associated with reduced body weight (kg), waist circumference (cm), and hip circumference (cm). Increased log high-density lipoprotein-cholesterol and decreased log interleukin 6 and C-reactive protein levels were observed. CONCLUSIONS: Ramadan fasting was associated with increased HDL-C levels and decreased anthropometric measurements and inflammatory cytokine levels. These results support the beneficial role of Ramadan fasting in reducing cardiometabolic abnormalities and inflammation in adults with diabetes.

Translation, validity, and reliability of an Arabic version of the dietary questionnaire on nutrition knowledge, self-efficacy, and practice among Arab young adults.

BACKGROUND AND OBJECTIVES: Assessing knowledge, self-efficacy, and practice among a given population us-ing a validated and reliable questionnaire is crucial. The aim of this study was to translate, validate, and test the reliability of the knowledge, self-efficacy, and practice in the Arabic population. Methods and Study De-sign: A previously published knowledge, self-efficacy, and practice nutrition questionnaire was translated and validated into Arabic. A panel of translation and nutrition experts from Arab countries participated in translation and testing validity. A convenience sampling technique was used to recruit participants across 22 Arab countries. An online self-administered questionnaire was completed twice with an interval of 2 weeks. Tests of validity (face and content) and reliability (consistency and test-retest reliability) were used. RESULTS: A total of 96 participants had a mean age of 21.5 years, 68.7% were female, and 80.2% were students. The mean expert proportional content validity index scale was 0.95, and intra class correlation values ranged from 0.59 to 0.76; all of these values were highly statistically significant at retest. CONCLUSIONS: The Arabic version of the questionnaire provided valid and reliable results for assessing knowledge, self-efficacy, and practice among Arab adolescents and young adults. This tool could assess nutritional education programs in a community setting and educational institutions targeting this population in Arab countries.

The influence of a diabetes awareness program on diabetes knowledge, risk perception, and practices among university students.

AIM: To compare diabetes mellitus (DM) knowledge, risk perception, and health practices before and after a mass awareness program among Princess Nourah bint Abdulrahman University students, Riyadh, Saudi Arabia. METHODS: A DM awareness program was conducted between November 2020 and February 2021 in three phases (total 13 weeks). The program comprised educational messages, short videos, virtual lectures, and peer-to-peer education. A total of 558 participants (before group, n = 360; after group, n = 198) were included in the study. RESULTS: Majority of the students had normal body mass index (BMI). The knowledge scores were significantly higher after the program (median 16, quartile range 13-18) than before (median 14, quartile range 12-16) (p < 0.001). No significant differences were observed in risk perception between the two groups (p > 0.05). In addition, increased moderate practice score and decreased poor practice score (p < 0.001) were observed. Factors influencing DM knowledge were higher education and awareness. Factors influencing health practices were higher BMI and knowledge after the program. CONCLUSION: DM awareness program improved DM knowledge and health practices among university students.

Dietary patterns among Saudis with type 2 diabetes mellitus in Riyadh: A cross-sectional study.

We investigated dietary patterns and their associations with sociodemographic and lifestyle factors in Saudi adults with type 2 diabetes mellitus (T2DM). A total of 297 participants (154 men and 143 women) with a mean age (standard deviation) of 54.0 (7.0) years were enrolled in the current study. Self-reported information on sociodemographic and lifestyle characteristics, eating behavior, and frequency of consumption was collected from all participants. Principal component analysis was used to determine dietary patterns. Multiple linear regressions were used to examine the associations between dietary patterns and sociodemographic and lifestyle factors. We identified five dietary patterns: "Vegetables and olive oil," "Refined grains and sweets," "Dairy products and legumes," "Dates and beverages," and "Fruit." Age was inversely associated with the pattern "Refined grains and sweets" (ß = -0.036, p <0.001). Females were more likely to have higher scores with the "Fruit" pattern (ß = 0.410, p = 0.011). Smoking was associated with "Vegetables and olive oil" (ß = 0.919, p = 0.001) and "Dates and beverages" (ß = -0.947, p = 0.001) patterns. The level of physical activity was associated with "Dates and beverages" (ß = 0.104, p = 0.048) and "Vegetables and olive oil" (ß = -0.102, p = 0.048) patterns. The number of snacks consumed was associated with "Dates and beverages" pattern (ß = -0.241, p = 0.005), and the frequency of meals eaten in fast food locations and restaurants per month was associated with "Vegetables and olive oil" pattern (ß = -0.043, p = 0.034). Each dietary pattern was associated with different sociodemographic and lifestyle factors and eating behaviors. This study provides insights into the underlying nutritional habits of Saudi Arabian patients with T2DM. Future studies are necessary to assess these associations in representative samples.

Evaluation of carbohydrate counting knowledge among individuals with type 1 diabetes mellitus in Saudi Arabia: a cross-sectional study.

Introduction: Carbohydrate counting (CC) is an important nutritional strategy to improve glycaemic outcomes among patients with diabetes. Few studies have investigated CC knowledge among individuals with type 1 diabetes mellitus (T1DM) in Saudi Arabia. Therefore, we aimed to evaluate CC knowledge in Saudi adults with T1DM. Study design and methods: A cross-sectional study was conducted between December 2021 and February 2022, including 224 patients with T1DM from the University Diabetes Center, Riyadh. Adults aged ≥18 years, diagnosed with T1DM for >1 year, and residing in Saudi Arabia were included. CC knowledge was assessed using a previously well-studied tool (AdultCarbQuiz), which was translated into Arabic and tested for validity by a group of dieticians. Descriptive statistics were used for data analysis, and bivariate and regression analyses were conducted. Results: The AdultCarbQuiz questionnaire-Arabic version had good validity and reliability (Cronbach's α: 0.87). The CC method was used by 54% of the participants. The mean CC knowledge score was 23.01±7.31. A significant negative linear relationship between the participants' CC knowledge scores, and age and glycated haemoglobin (HbA1c) levels, was revealed by simple regression analysis. Furthermore, significant independent variables related to CC knowledge scores were CC use, HbA1c levels, being taught about CC (>5 times), insulin pump usage and DM duration (≤15 years). Conclusions: Approximately half of the patients used the CC method. The mean CC knowledge scores were better in patients who used the CC method, were more frequently taught about CC, were treated using an insulin pump, and had a shorter DM duration than their counterparts. Therefore, designing and implementing a well-structured nutrition education programme tailored to individuals with diabetes is crucial to provide them with up-to-date dietary information, as well as the necessary knowledge and skills, to improve their outcomes and manage their condition.

Anthropometric cutoff values for predicting metabolic syndrome in a Saudi community: from the SAUDI-DM study.

BACKGROUND: The prevalence of metabolic syndrome varies widely by ethnicity and by the criteria used in its definition. OBJECTIVE: To identify the optimal cutoff values for waist circumference (WC), waist-to-hip ratio (WHR) and body mass index (BMI) for identifying metabolic syndrome among the Saudi population. DESIGN: Nationwide household cross-sectional population-based survey. SETTING: Thirteen health sectors in Saudi Arabia. SUBJECTS AND METHODS: We used data for subjects in the Saudi Abnormal Glucose Metabolism and Diabetes Impact Study (SAUDI-DM), which was conducted from 2007 to 2009. Using International Diabetes Federation (IDF) criteria, metabolic syndrome and its different components were assessed using anthropometric measurements, blood pressure, fasting plasma glucose, triglycerides and HDL cholesterol. Receiver operating characteristic (ROC) curves were generated to assess sensitivity and specificity for different cutoff values of WC, WHR, and BMI. The Youden index was used to calculate the optimal cutoff value for each anthropometric measurement. MAIN OUTCOME MEASURE(S): Optimal cutoff value for WC, WHR, and BMI for identifying the risk of metabolic syndrome. RESULTS: The prevalence of two or more risk factors for metabolic syndrome was observed in 43.42% of the total cohort of 12126 study participants >=18 years of age. The presence of two or more risk factors were significantly higher among men (46.81%) than women (40.53%) (P < .001). The optimal cutoff values for WC, WHR, and BMI were 92 cm, 0.89, and 25 kg/m2 for men and 87 cm, 0.81 and 28 kg/m2 for women for identifying the risk of metabolic syndrome. The prevalence of elevated triglycerides, blood pressure, and fasting plasma glucose significantly increased with age for both genders. CONCLUSIONS: The proposed WC cutoff values were better than WHR and BMI in predicting metabolic syndrome and could be used for screening people at high risk for metabolic syndrome in the Saudi population. LIMITATIONS: No direct measure of body fatness and fat distribution, cross-sectional design.

Vitamin B12 insufficiency induces cholesterol biosynthesis by limiting s-adenosylmethionine and modulating the methylation of SREBF1 and LDLR genes.

BACKGROUND: The dietary supply of methyl donors such as folate, vitamin B12, betaine, methionine, and choline is essential for normal growth, development, and physiological functions through the life course. Both human and animal studies have shown that vitamin B12 deficiency is associated with altered lipid profile and play an important role in the prediction of metabolic risk, however, as of yet, no direct mechanism has been investigated to confirm this. RESULTS: Three independent clinical studies of women (i) non-pregnant at child-bearing age, (ii) in early pregnancy, and (iii) at delivery showed that low vitamin B12 status was associated with higher total cholesterol, LDL cholesterol, and cholesterol-to-HDL ratio. These results guided the investigation into the cellular mechanisms of induced cholesterol biosynthesis due to vitamin B12 deficiency, using human adipocytes as a model system. Adipocytes cultured in low or no vitamin B12 conditions had increased cholesterol and homocysteine levels compared to control. The induction of cholesterol biosynthesis was associated with reduced s-adenosylmethionine (AdoMet)-to-s-adenosylhomocysteine (AdoHcy) ratio, also known as methylation potential (MP). We therefore studied whether reduced MP could lead to hypomethylation of genes involved in the regulation of cholesterol biosynthesis. Genome-wide and targeted DNA methylation analysis identified that the promoter regions of SREBF1 and LDLR, two key regulators of cholesterol biosynthesis, were hypomethylated under vitamin B12-deficient conditions, and as a result, their expressions and cholesterol biosynthesis were also significantly increased. This finding was further confirmed by the addition of the methylation inhibitor, 5-aza-2'-deoxycytidine, which resulted in increased SREBF1 and LDLR expressions and cholesterol accumulation in vitamin B12-sufficient conditions. Finally, we observed that the expression of SREBF1, LDLR, and cholesterol biosynthesis genes were increased in adipose tissue of vitamin B12 deficient mothers compared to control group. CONCLUSIONS: Clinical data suggests that vitamin B12 deficiency is an important metabolic risk factor. Regulation of AdoMet-to-AdoHcy levels by vitamin B12 could be an important mechanism by which it can influence cholesterol biosynthesis pathway in human adipocytes.

Variation in macro and trace elements in progression of type 2 diabetes.

Macro elements are the minerals of which the body needs more amounts and are more important than any other elements. Trace elements constitute a minute part of the living tissues and have various metabolic characteristics and functions. Trace elements participate in tissue and cellular and subcellular functions; these include immune regulation by humoral and cellular mechanisms, nerve conduction, muscle contractions, membrane potential regulations, and mitochondrial activity and enzyme reactions. The status of micronutrients such as iron and vanadium is higher in type 2 diabetes. The calcium, magnesium, sodium, chromium, cobalt, iodine, iron, selenium, manganese, and zinc seem to be low in type 2 diabetes while elements such as potassium and copper have no effect. In this review, we emphasized the status of macro and trace elements in type 2 diabetes and its advantages or disadvantages; this helps to understand the mechanism, progression, and prevention of type 2 diabetes due to the lack and deficiency of different macro and trace elements.

Sleep duration and quality associated with obesity among Arab children.

The link between sleep duration and obesity has been well established in adults, but several epidemiological studies revealed inconsistent findings in adolescents and younger children. This study aimed to investigate the relationship between sleep length and obesity in Saudi students. A total of 5,877 Saudi students, boys (55.2%) and girls (44.8%), aged between 10 and 19 years were randomly selected from elementary, intermediate, and secondary schools in different regions of Riyadh. A questionnaire on sleep behaviors was given. Anthropometry included BMI and waist and hip circumferences. Sleeping