Pre-harvest sprouting resistance and haplotype variation of ThVp-1 gene in the collection of wheat-wheatgrass hybrids.

The germplasm collection of 87 wheat-wheatgrass hybrids developed in Tsitisin Main Botanical Garden (Russia, Moscow) was evaluated for resistance to pre-harvest sprouting (PHS) by spike sprouting (SS) and germination index (GI) assays as well as for spike and grain features. The PHS resistance variation and haplotype polymorphism of the wheatgrass ThVp-1 and wheat TaVp-1B genes orthologues of Vp-1 was revealed in the studied collection. Four haplotypes of ThVp-1 were revealed: ThVp-1a (41% of the entries), ThVp-1b (13%), ThVp-1c (29%), and ThVp-1d (15%). The association between the allelic state of ThVp-1 and PHS resistance in the wheat-wheatgrass hybrids was shown: haplotype ThVp-1d of the wheatgrass Vp-1 gene is significantly associated with reduced PHS in the wheat-wheatgrass hybrids (mean SS 0.33, mean GI 0.64). The resistant entries may be perspective as a source of PHS resistance in the development of commercial cultivars of perennial wheat.

[Distal reconstructions under critical lower limb ischemia in elderly patients].

Performong reconstructive operations on arteriae is the only way to avoid limb amputation in patients with critical limb ischemia. Whereas reconstructive manipulations on distal arteriae, especially in patients older then 75 years, are often entailed by thrombotic complications. High peripheric vessel resistance is considered to be the crucial factor of the bypass thrombosis. The conducted study demonstrated the efficacy of the use of vena saphena magna collateral tributaries as bypass unload collectors. Femoral distal autovenous in situ reconstruction decreases vessel resistance. Due to the arterial reconstruction with bypass unload using unligated autovenous tributaries the cumulative bypass patency was 75,1% a year after the procedure. 5-year cumulative bypass patency was 53,8%. 5 - year lethality was 28,6%.