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We present finite-difference time domain simulations and optical characterizations via micro-photoluminescence measurements of InP-based L4/3 photonic crystal cavities with embedded quantum dots (QDs) and designed for the M1 ground mode to be emitting at telecom C-band wavelengths. The simulated M1 Q-factor values exceed 106, while the M1 mode volume is found to be 0.33 × (λ/n)3, which is less than half the value of the M1 mode volume of a comparable L3 cavity. Low-temperature micro-photoluminescence measurements revealed experimental M1 Q-factor values on the order of 104 with emission wavelengths around 1.55 µm. Weak coupling behavior of the QD exciton line and the M1 ground mode was achieved via temperature-tuning experiments.
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We investigate the optical modes in a coupled pair of semiconductor microdisks in symmetric and asymmetric configurations both experimentally and theoretically. While the quality factors of coupled first- and second-order whispering gallery modes (WGMs) show a conventional crossing, the quality factors of the same-order WGMs reveal an interesting splitting behavior, leading to the formation of high- and low-quality supermodes. Our results are reproduced by numerical simulations, and an explanation based on optical interference is suggested. Quality-factor splitting is a subtle phenomenon that might help to design microarchitectures for efficient optical coupling in cavity quantum electrodynamic experiments.
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We study the effect of an external biaxial stress on the light emission of single InGaAs/GaAs(001) quantum dots placed onto piezoelectric actuators. With increasing compression, the emission blueshifts and the binding energies of the positive trion (X+) and biexciton (XX) relative to the neutral exciton (X) show a monotonic increase. This phenomenon is mainly ascribed to changes in electron and hole localization and it provides a robust method to achieve color coincidence in the emission of X and XX, which is a prerequisite for the possible generation of entangled photon pairs via the recently proposed "time reordering" scheme.
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We have studied the emission properties of single CdTe/ZnTe quantum dots (QDs) grown on Si(001) substrates by using molecular beam epitaxy and atomic layer epitaxy. The good quality of the QDs is attested by the resolution-limited emission, negligible background and absence of measurable spectral jitter or blinking. Power-dependent, polarization-dependent, and temperature-dependent microphotoluminescence spectroscopy measurements were performed to identify the exciton, the biexciton, and two oppositely charged excitons in the emission spectra of single QDs.
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We have studied some features of K+ accumulation by glycolysing Mycoplasma mycoides var. Capri cells. We report that when Na+ is absent from the external medium, K+ accumulates up to the level predicted by the amplitude of the transmembrane electrical potential, delta psi m, measured by Rb+ and methyltriphenylphosphonium cation (TPMP+) distribution. Therefore, under these experimental conditions, the coupling mechanism of K+ uptake consists of a delta psi m-driven uniport. More important, when Na+ is present in the external medium, the level of K+ accumulation by glycolysing Mycoplasma cells is far too steep to be equilibrium with delta psi m (-120 mV for delta muK+ compared with -90mV for delta muRb+ or delta muTPMP+). Our results clearly indicate the presence in Mycoplasma of an active K+-transport system specifically stimulated by Na+. Furthermore, by controlling the amplitude of the energy-dependent delta muH+, we obtain strong evidence that this specific Na+-stimulated K+ transport is modulated by the transmembrane electrical potential. Finally, we show that ATP is consumed when such a transport system is in activity.
Assuntos
Mycoplasma mycoides/metabolismo , Potássio/metabolismo , Sódio/farmacologia , Trifosfato de Adenosina/metabolismo , Transporte Biológico Ativo/efeitos dos fármacos , Cátions Monovalentes/farmacologia , Potenciais da Membrana/efeitos dos fármacos , Mycoplasma mycoides/citologia , Mycoplasma mycoides/efeitos dos fármacos , Ácidos Fosfóricos/farmacologiaRESUMO
We have studied the links between the mechanisms of Na(+), K(+) and H(+) movements in glycolysing Mycoplasma mycoides var. Capri cells. In the light of the results reported in the preceding paper [Benyoucef, Rigaud & Leblanc (1982) Biochem. J.208, 529-538], we investigated certain properties of the membrane-bound ATPase of Mycoplasma cells, with special reference to its ionic requirements and sensitivity to specific inhibitors. Our findings show, first, that, although Na(+) stimulated ATPase activity, K(+) did not affect it, and, secondly, that NN'-dicyclocarboidi-imide and 7-chloro-4-nitrobenzo-2-oxa-1,3-diazole (NBD) were potent inhibitors of the basal ATPase activity, which was unaffected by vanadate and ouabain. We also investigated the movements of Na(+) and H(+) under the experimental conditions applied to the study of the K(+) uptake reported in the preceding paper, and found that when ;Na(+)-loaded cells' previously equilibrated with (22)Na(+) were diluted in a sodium-free medium, addition of glucose induced a rapid efflux of (22)Na(+). This energy-dependent efflux was independent of the presence of KCl in the medium. Studies of the changes in internal pH by 9-aminoacridine fluorescence or [(14)C]methylamine distribution indicated that the movement of Na(+) was coupled to that of protons moving in the opposite direction, a finding that supports the presence of an Na(+)/H(+) antiport. When Na(+)-loaded cells are diluted in an Na(+)-rich medium the Na(+)/H(+) antiport is still active, but cannot decrease the intracellular Na(+) concentration. Under such conditions, net (22)Na(+) extrusion is specifically dependent on the presence of K(+) in the medium. The present results and those derived from the study of K(+) accumulation (the preceding paper) can be rationalized by assuming that Mycoplasma mycoides var. Capri cells contain two transport systems for Na(+) extrusion: an Na(+)/H(+) antiport and an ATP-consuming Na(+)/K(+)-exchange system.
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Mycoplasma mycoides/metabolismo , Potássio/metabolismo , Sódio/metabolismo , Adenosina Trifosfatases/metabolismo , Transporte Biológico Ativo/efeitos dos fármacos , Cátions Monovalentes/farmacologia , Membrana Celular/efeitos dos fármacos , Membrana Celular/metabolismo , Metabolismo Energético/efeitos dos fármacos , Modelos Biológicos , Mycoplasma mycoides/citologia , Mycoplasma mycoides/efeitos dos fármacosRESUMO
The electrochemical proton gradient, delta mu H+ generated upon glycolysis by Mycoplasma mycoides var. Capri cells has been determined. The components, the transmembrane pH gradient, delta pH, and the membrane potential, delta psi, were measured using several methods. The determination of the delta pH was conducted by measuring the transmembrane distribution of weak acids (acetate and butyrate) and of a weak base (methylamine), using flow dialysis and filtration techniques. The transmembrane electrical potential was determined from the distribution of the lipophilic cation Ph3MeP+ and of Rb+ or K+ in the presence of valinomycin. At extra-cellular pH 7.2, glycolyzing Mycoplasma cells maintain an internal pH more alkaline (0.5 pH unit) than that of the milieu and an electrical potential of - 85 mV, interior negative. The delta mu H+ in M. mycoides var. Capri cells is thus about - 115 mV. When the external pH was altered from 7.7 to 5.7 delta psi decreased from - 90 mV to - 60 mV. On other hand although the internal pH decreased, delta pH was found to increase from 0.2 to 1.0 pH unit. Since the changes in delta psi were largely compensated by the changes in delta pH, delta mu H+ remained practically constant at about - 115 mV throughout the pH range tested. Finally, inhibition of delta pH by N,N'-dicyclohexylcarbodiimide, carbonylcyanide-p-trifluoromethoxyphenylhydrazone or nigericin confirmed that chemiosmotic phenomena contribute to energy transduction across the membranes of M. mycoides var. Capri cells.
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Mycoplasma mycoides/metabolismo , Transporte Biológico Ativo/efeitos dos fármacos , Carbonil Cianeto p-Trifluormetoxifenil Hidrazona/farmacologia , Dicicloexilcarbodi-Imida/farmacologia , Eletroquímica , Concentração de Íons de Hidrogênio , Potenciais da Membrana , Nigericina/farmacologia , Potássio/metabolismo , Prótons , Rubídio/metabolismoRESUMO
The results presented show that in Mycoplasma mycoides var. Capri, regulation of glucose uptake by its non-metabolizable analogue methyl alpha-D-glucoside, can be used to control intracellular ATP content. This in turn leads to a control of the rate of proton extrusion catalysed by the Mg2+-dependent ATPase (phi (cHxN)2C H+) and the respective amplitudes of the components of delta mu H+. When Mycoplasma cells are incubated with 10 mM methyl alpha-D-glucoside, the amplitude of phi (cHxN)2C H+, of the electrical potential delta psi and of the chemical gradient delta pH become continuous functions of external glucose concentration within the limits of the non-energized and fully energized states. Analysis of the relationships between graduated amplitudes of delta psi, delta pH and phi (cHxN) 2C H+ show that the primary form of energy stored by a delta mu H+ generator is the electrical potential.
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Mycoplasma mycoides/metabolismo , Trifosfato de Adenosina/metabolismo , Transporte Biológico Ativo , Eletroquímica , Metabolismo Energético , Glucose/metabolismo , Glucose/farmacologia , Concentração de Íons de Hidrogênio , Potenciais da Membrana/efeitos dos fármacos , Metilglucosídeos/farmacologia , PrótonsRESUMO
The diffusion coefficients D(cm2.s-1) of the sodium salts of a series of hydrophilic mono- and dicarboxylic acids, have been measured in the hydrophilic layers of phosphatidylcholine-water lamellar phases, as a function of phase hydration. At pH 9.0, the diffusion rates of the anionic (RCOO-) form of the acid exhibit a prominent increase within a narrow range of water content, specific to each anion. This high diffusion rate seems to occur when the Stokes diameter of an anion is equal to the thickness of the aqueous layer between the two planes formed by the quaternary ammonium groups of the choline phosphate dipoles of two facing layers of phosphatidylcholine molecules. This phenomenon demonstrates the importance of the spatial organization of successive binding sites in the rate constant of diffusional processes in hydrophilic channels.