Comparison of methods to measure body fat in 7-to-10-year-old children: a systematic review.

OBJECTIVE: To investigate methodological aspects in body fat (BF) measurements in 7-to-10-year-old children. STUDY DESIGN: Systematic review of the literature. METHODS: The studies were chosen from the PubMed and Scielo databases according to a protocol that defined: inclusion criteria; a search and quality-assessment strategy; and information extraction. RESULTS: 27 studies published from 2004 to 2014 were included. The literature describes skinfold measurements and dual energy X-ray absorptiometry (DEXA) as being the reference methods most widely used in the assessment of the ability of methods to identify BF. The most commonly-used statistical analyses were the Pearson correlation coefficient, and sensitivity and specificity performance analyses. The comparison between the tested methods and the references showed that body mass index (BMI) and waist circumference (WC) are strongly correlated to BF as calculated by bioelectrical impedance or skinfolds, and that there is a moderate positive correlation with percent body fat as calculated by DEXA, air-displacement plethysmography (ADP) or isotope dilution. There was a moderate positive correlation between waist-to-height ratio (WHtR) and BF, as estimated by ADP and skinfolds. Performance studies suggest that BMI and WC are very specific but less sensitive methods. CONCLUSIONS: The results of this systematic review show favourable evidence for the use of anthropometric indicators - above all BMI and WC- in the measurement of BF, when more accurate techniques such as DEXA and ADP are not feasible. They also demonstrate features that make them advantageous for epidemiological studies in a child population, since they are easy and safe to obtain and well tolerated by the children.

Prevalence of Neospora caninum antibodies and factors associated with their presence in dairy cattle of the north of Paraná state, Brazil.

To determine the prevalence of Neospora caninum antibodies and associated factors, blood sera from 623 female dairy cattle from 23 farms in the north of the state of Paraná, Brazil, were analyzed by means of the indirect immunofluorescent-antibody test (IFAT > or = 25). Serum samples from 134 dogs living on the same farms also were tested for N. caninum antibodies (IFAT > or = 50), and the presence of dogs was associated with the prevalence of N. caninum antibodies in cattle. The overall seroprevalence in cattle was 14.3%, mainly in animals over 24 months of age. Seroprevalence in Holsteins (15.1% of 558) was greater than in mixed-breed cattle (7.7% of 65). Age (> or =24 months) of cattle, feeding silage and/or concentrate produced on the farm were associated; antibodies were found in 21.6% of dogs; and the presence of dogs was associated with the prevalence of N. caninum antibodies in cattle.

Occurrence of anti-Toxoplasma gondii antibodies in dogs in the urban area of Monte Negro, Rondônia, Brazil.

The prevalence of anti-Toxoplasma gondii antibodies in dogs in an urban area of the municipality of Monte Negro, Rondônia, Brazil, was evaluated using an indirect immunofluorescent antibody test (IFAT). Blood samples were taken from 157 dogs living in 85 of the 94 blocks of the city. A seropositivity of 76.4% (120/157) was found and associations between the prevalence and the variables sex, age, type of raising and food were studied. The prevalence tended to increase with age (p < 0.05); dogs over 24 months old had 85.5% (100/117) positivity, compared with 50% (20/40) in dogs less than 24 months old, showing postnatal exposure to the agent. It was also observed that dogs with access to the streets showed greater prevalence (84.9%) than companion animals (58.8%). There was no association between sex or the type of food (home-made or commercial) and anti-T. gondii antibodies.

Prevalence of antibodies to Neospora caninum in dogs from Amazon, Brazil.

Neospora caninum is an important cause of abortion in dairy cattle worldwide. Dogs are important in the epidemiology of this parasite because they are the only hosts known to excrete N. caninum oocysts. Antibodies to N. caninum were assayed in serum samples from 157 dogs from Monte Negro, Rondônia, Amazon, Brazil using the indirect fluorescent antibody test. Antibodies to N. caninum were found in 13 (8.3%) of dogs in titers of 1:50 in 1, 1:100 in 2, 1:200 in 5, 1:800 in 1, 1:1600 in 2, and 1:3200 in 2 dogs. These data indicate that N. caninum infection is prevalent even in remote areas of the Amazon.

[Comparative study between larval surveys and ovitraps to monitor populations of Aedes aegypti]. / Comparação entre pesquisa larvária e armadilha de oviposição, para detecção de Aedes aegypti.

This study compares two techniques for detecting the presence of Aedes aegypti: larval surveys and the oviposition trap. In two areas of the municipality of Salvador, Bahia, Brazil were investigated 5,026 households. Larval surveys and oviposition traps were used simultaneously in these households. Different positivity levels (larvae and/or eggs) were detected between and within the two areas. However, only the use of the oviposition trap detected a significant statistical difference between the areas (z = 9,520, p < 0.001). Comparison of the Breteau, Household and Oviposition Indices reveals greater power of detection of positivity in the oviposition trap. There were prevalence ratios of positivity for oviposition trap of 3.4 and 2.1 (for areas 1 and 2 respectively) when compared with larval surveys. The oviposition trap proved to be an economical and operationally viable method, and the most effective in the surveillance of this species.

Reproducibility of alkaline antigens of Leishmania major-like and Leishmania (V.) braziliensis evaluated by IgG-ELISA. Comparison of antigens added of a protein inhibitor (PMSF) or not.

This paper deals with the analysis of 10 batches of L.major-like and L. (V.) braziliensis antigens added or not of a proteases inhibitor evaluated by means of an IgG-ELISA on three consecutive days using positive standard sera from patients with diagnosis of American Leishmaniasis previously tested for the presence of IgG antibodies by means of ELISA. The statistical analysis showed that for L. (V.) braziliensis the PMSF-containing antigen did not show any difference among batches or days of testing; the L. (V.) braziliensis antigen without PMSF showed statistical significance for differences among batches and a two-way ANOVA showed significant differences between antigens. L.major-like antigen prepared with or without PMSF showed differences among batches; all 3 days of testing displayed differences for the PMSF antigen but only for days 1 and 2 for the antigen without inhibitor. A two-way ANOVA showed differences among batches of the antigens but not for antigens with and without the protein inhibitor. According to the statistical analysis the L.major-like antigen added or not of PMSF has shown that it is the choice antigen for mucocutaneous leishmaniasis serology.