RESUMO
This study aimed to develop chitosan films incorporating natural antioxidants from peanut skin (EPS) and pink pepper residue (EPP) extracts, as well as to evaluate their effects on lipid oxidation, pH, color, and microbial counts of a restructured chicken product. EPS had higher phenolic content and antioxidant activity compared to EPP. When both extracts were applied to chicken meat and the chitosan films, there were no differences for color, pH and total mesophilic counts compared to control at the end of the storage period. For lipid oxidation (peroxide value and thiobarbituric acid reactive substances), both extracts proved to be as effective as butylated hydroxytoluene to maintain the oxidative stability of the chicken product. The microbial counts of psychrotrophic microorganisms were significantly lower for treatments with active films. Chitosan active films with residue extracts may maintain the quality of chicken products due to their antioxidant and antimicrobial potential.
Assuntos
Antioxidantes/química , Quitosana/química , Embalagem de Alimentos/métodos , Conservação de Alimentos/métodos , Conservantes de Alimentos/química , Resíduos Industriais , Indústria de Embalagem de Carne/métodos , Produtos Avícolas/análise , Anti-Infecciosos/química , Anti-Infecciosos/isolamento & purificação , Antioxidantes/isolamento & purificação , Arachis , Bactérias/efeitos dos fármacos , Bactérias/crescimento & desenvolvimento , Cor , Microbiologia de Alimentos/métodos , Conservantes de Alimentos/isolamento & purificação , Concentração de Íons de Hidrogênio , Peroxidação de Lipídeos , Lipídeos/química , Nozes , Oxirredução , Piper , Produtos Avícolas/microbiologia , Substâncias Reativas com Ácido Tiobarbitúrico/química , Fatores de TempoRESUMO
BACKGROUND: Guava pomace is an example of the processing waste generated after the manufacturing process from the juice industry that could be a source of bioactives. Thus, the present investigation was carried out in order to evaluate the anti-inflammatory and antinociceptive potential and determinate the main phenolic compounds of a guava pomace extract (GPE). METHODS: The anti-inflammatory activity was evaluated by carrageenan, dextran, serotonin, histamine-induced paw edema and neutrophils migration in the peritoneal cavity models. Acetic acid-induced abdominal writhing and formalin test were performed to investigate the antinociceptive effects. In addition, the content of total phenolic and of individual phenolic compounds was determined by GC/MS. RESULTS: GPE showed anti-inflammatory activity by carrageenan, dextran, serotonin, histamine-induced paw edema and neutrophils migration in the peritoneal cavity models (p < 0.05). GPE also demonstrated antinociceptive activity by acetic acid-induced abdominal writhing and formalin test (p < 0.05). The total phenolic value was 3.40 ± 0.09 mg GAE/g and epicatechin, quercetin, myricetin, isovanilic and gallic acids were identified by GC/MS analysis. CONCLUSIONS: The presence of bioactive phenolic compounds as well as important effects demonstrated in animal models suggest that guava pomace could be an interesting source of anti-inflammatory and analgesic substances.
Assuntos
Analgésicos/farmacologia , Anti-Inflamatórios/farmacologia , Cavidade Peritoneal/citologia , Extratos Vegetais/farmacologia , Psidium/química , Analgésicos/química , Animais , Anti-Inflamatórios/química , Comportamento Animal/efeitos dos fármacos , Formaldeído , Resíduos Industriais , Masculino , Camundongos , Camundongos Endogâmicos BALB C , Neutrófilos , Medição da Dor , Extratos Vegetais/químicaRESUMO
Vegetables are widely consumed in Brazil and exported to several countries. This study was performed to evaluate the phenolic content and antioxidant activity of vegetables commonly consumed in Brazil using five different methods, namely DPPH and ABTS free radical, ß-carotene bleaching, reduction of Fe(3+) (FRAP), oxidative stability in Rancimat, and the chemical composition using gas chromatography-mass spectrometry (GC-MS). The content of phenolic compounds ranged from 1.2 mg GA/g (carrot) to 16.9 mg GA/g (lettuce). Vegetables presenting the highest antioxidant activity were lettuce (77.2 µmol Trolox/g DPPH(â¢); 447.1 µmol F(2+)/g FRAP), turmeric (118.6 µmol Trolox/g ABTS(â¢+); 92.8% ß-carotene), watercress and broccoli (protective factor 1.29-Rancimat method). Artichoke, spinach, broccoli, and asparagus also showed considerable antioxidant activity. The most frequent phenolic compounds identified by GC-MS were ferulic, caffeic, p-coumaric, 2-dihydroxybenzoic, 2,5-dihydroxybenzoic acids, and quercetin. We observed antioxidant activity in several vegetables and our results point out their importance in the diet.