1.
J Am Med Dir Assoc
; 18(12): 1089-1091, 2017 12 01.
Artigo
em Inglês
| MEDLINE
| ID: mdl-29169738
Assuntos
Demência/terapia , Casas de Saúde , Agitação Psicomotora/terapia , Terapias Sensoriais através das Artes/métodos , Idoso , Idoso de 80 Anos ou mais , Estudos Cross-Over , Terapia por Exercício , Feminino , Humanos , Masculino , Projetos Piloto , Estudos Prospectivos , Resultado do Tratamento
2.
Biotechniques
; 56(4): 186-93, 2014.
Artigo
em Inglês
| MEDLINE
| ID: mdl-24724844
RESUMO
Cell-free protein synthesis (CFPS) is a versatile tool for rapid recombinant protein production and engineering. One drawback of cell-free technology is the necessity to store the major components-cell extracts and energy systems-below freezing in bulky aqueous solutions. Here we describe simple methods for lyophilizing extracts and preparing powdered energy systems for CFPS. These techniques allow for high-density storage of cell-free systems that are more robust against temperature and bacterial degradation. Our methods have the potential to decrease storage expenses, allow for longer shelf-life of cell extracts at room temperature, and enable durable portable protein production technologies.