Dentinal Microcracks after Root Canal Preparation in Primary Root: An In Vitro Evaluation of ProTaper Gold and Kedo-S Rotary File Systems.

Context: There is a possibility of dentinal crack formation in primary teeth after root canal preparation using rotary files. Aims: To evaluate and compare the effect of ProTaper-Gold and Kedo-S rotary files on crack formation after root canal preparation in primary molars. Settings and design: A total of 120 freshly extracted mandibular primary molars (6-9 years) were randomly divided into three groups of 40 each: ProTaper-Gold, Kedo-S, and Hand H-files, respectively. Materials and methods: The roots were covered with a snuggly fitting surgical glove and stabilized in the teeth slot of a silicone mold of mandibular mixed dentition. Dental casts were obtained in a mixture of plaster of paris and sawdust. A screw system was incorporated in the cast for stabilization of the cast into the phantom head. All the root canals were instrumented in a standard operating position till 1 mm short of the radiographic apex. All roots were then stained and sectioned perpendicular to the long axis at the furcation level and 2 mm below the furcation to obtain one section per tooth. Sections were examined under a stereomicroscope at 25× magnification for any crack formations and recorded. Data were analyzed using Wilcoxon signed-rank and Kruskal-Wallis tests (p = 0.05). Results: The total number of cracks in terms of percentage following the use of ProTaper Gold, Kedo-S, and H-files were 35, 10, and 0%, respectively, on the upper surface and 15, 5, and 0% on the lower surface. Within the group, there was a statistically significant difference in ProTaper-Gold (p = 0.001). Conclusion: The use of ProTaper-Gold resulted in a greater number of dentinal cracks compared to Kedo-S and H-files. How to cite this article: Patil MB, Mandroli PS, Jalannavar P, et al. Dentinal Microcracks after Root Canal Preparation in Primary Root: An In Vitro Evaluation of ProTaper Gold and Kedo-S Rotary File Systems. Int J Clin Pediatr Dent 2023;16(5):692-697.

Toward a Tripartite Model and Measurement of Proactive Personality.

Although proactive personality (Bateman & Crant, 1993), as a relatively stable disposition to bring about change in one's environment, is a central construct in the management and organizational behavior literature, questions have arisen about its definition and structure. To answer these questions, we present a tripartite model of proactive personality consisting of 3 dimensions: perception (i.e., perceiving opportunities for change), implementation (i.e., planning and executing change), and perseverance (i.e., persisting until the change is realized). Exploratory factor analysis informed the construction of a 14-item scale, and confirmatory factor analyses supported the validity of the scale. The new scale demonstrated satisfactory reliability and accounted for significant variance in task performance above that of original proactive personality, personal initiative, conscientiousness, and social desirability. Tripartite proactive personality might provide a more nuanced view of dispositional proactivity and greater predictive power than the original unidimensional proactive personality.

Synaptic Phospholipid Signaling Modulates Axon Outgrowth via Glutamate-dependent Ca2+-mediated Molecular Pathways.

Altered synaptic bioactive lipid signaling has been recently shown to augment neuronal excitation in the hippocampus of adult animals by activation of presynaptic LPA2-receptors leading to increased presynaptic glutamate release. Here, we show that this results in higher postsynaptic Ca2+ levels and in premature onset of spontaneous neuronal activity in the developing entorhinal cortex. Interestingly, increased synchronized neuronal activity led to reduced axon growth velocity of entorhinal neurons which project via the perforant path to the hippocampus. This was due to Ca2+-dependent molecular signaling to the axon affecting stabilization of the actin cytoskeleton. The spontaneous activity affected the entire entorhinal cortical network and thus led to reduced overall axon fiber numbers in the mature perforant path that is known to be important for specific memory functions. Our data show that precise regulation of early cortical activity by bioactive lipids is of critical importance for proper circuit formation.

Evaluation of Culture, Antigen Detection and Polymerase Chain Reaction for Detection of Vaginal Colonization of Group B Streptococcus (GBS) in Pregnant Women.

BACKGROUND AND OBJECTIVE: Group B Streptococcal infection is an important cause of neonatal morbidity and mortality. Early detection of perinatal vagino-rectal (VR) carriage of Group B Streptococcus (GBS) is important in the management of newborn infections. The objective of the study was to evaluate Culture, antigen detection and Polymerase chain reaction for detection of GBS in Pregnant women. SETTINGS AND DESIGN: Observational descriptive study was done in a tertiary care hospital in Southern India. MATERIALS AND METHODS: VR swabs were collected from 50 women at 35 to 37 weeks of gestation. Culture in a selective Lim enrichment broth with subsequent culture on 5% sheep blood agar, Conventional PCR assay and antigen detection method were performed. The performance of antigen detection and PCR methods were compared with culture. STATISTICAL ANALYSIS: STATISTICAL ANALYSIS was performed by Chi-Square test. RESULTS: GBS cultures were positive for 16% of the specimen (8 out of 50). Considering culture as a gold standard, Sensitivity, Specificity, Positive predictive value and Negative predictive value of antigen detection was 100%, 92.86%, 72.73%, 100% and similarly for that of PCR was 100%, 45.23%, 25.80%, 100%, respectively. CONCLUSION: Antigen detection method was the rapid, sensitive and specific test for the detection of GBS colonizers during pregnancy.

Comparison of various culture methods for isolation of group B streptococcus from intrapartum vaginal colonization.

AIMS: Group B Streptococcus (GBS) is one of the most common causes of neonatal sepsis throughout the world. Reports of vaginal colonization of GBS in India are few and variable. A study was conducted on pregnant women in a tertiary care hospital to compare various methods for isolation of GBS, to study the prevalence of GBS in pregnant women in third trimester, and to determine risk factors for GBS colonization. SETTINGS AND DESIGN: Observational descriptive study. MATERIALS AND METHODS: High vaginal swabs from 150 pregnant women in their third trimester were used to compare three methods for isolation of GBS viz. direct culture on 5% Sheep Blood agar, direct culture on selective Columbia Blood Agar and culture in LIM enrichment broth with subsequent culture on 5% Sheep Blood agar. A history of associated risk factors was also taken. STATISTICAL ANALYSIS USED: Statistical analysis was performed by Chi-square test. RESULTS: Isolation was best from LIM enrichment broth with subsequent culture on 5% Sheep Blood Agar. Prevalence of GBS colonization by using culture method was 12.67%. Most frequently associated risk factor was intrapartum fever (42.11%). CONCLUSIONS: Standard Culture Method using LIM enrichment should be adopted as standard practice for isolation of GBS from vaginal swabs.

Potential of Vitex negundo roots in the treatment of ulcerative colitis in mice.

CONTEXT: Vitex negundo Linn. (Verbenaceae) is an indigenous tree species in India. This tree species has been of interest to researchers because traditionally its roots are reported in the treatment of ulcer and colic pain. OBJECTIVE: The present work was undertaken to validate its folk use in the treatment of ulcerative colitis (UC) by using the method of acetic acid-induced colitis in mice. MATERIALS AND METHODS: Ethanol and aqueous extracts of roots of V. negundo (100 mg/kg) were screened for use in the treatment of UC by the method of acetic acid-induced UC in mice. Macroscopical study of the colon, level of myeloperoxidase (MPO), malondialdehyde (MDA) in colon tissue and blood and histopathology of the colon tissue were studied for the assessment. RESULTS: Ethanol extract (100 mg/kg) reduced the level of MPO in blood from 355 ± 0.39 to 240 ± 0.36 U/mL and from 385 ± 0.35 to 257 ± 0.36 U/mg in tissue. Similarly, it reduced the level of MDA in blood from 9.40 ± 0.42 to 6.10 ± 0.36 nmol/mL and from 9.38 ± 0.56 to 5.89 ± 0.56 U/mg in tissue. Both the results are comparable with the standard drug, prednisolone (5 mg/kg). This preventive effect was observed by morphological and histopathological study. DISCUSSION AND CONCLUSION: Results showed that ethanol extract of V. negundo root is effective in the treatment of UC and results are comparable with the standard drug, prednisolone, and thus possessing a great potential in the treatment of UC.

Anti-implantation effect of a carbamate fungicide mancozeb in albino mice.

Mancozeb, an organocarbamate fungicide, was administered to examine the effect on implantation at doses of 18, 24, 30 and 36 mg/kg body weight/d to normal virgin swiss albino mice for 8 days to pregnant mice. The vaginal smear and body weight of the mice were recorded daily and mice were sacrificed on 9th day of pregnancy. There was a complete inhibition of implantation in 36 mg mancozeb treated mice with 100% pre-implantation loss. There was a partial inhibition of implantation in 24 and 30 mg mancozeb treated mice with 53.44 and 90.16% pre-implantation loss respectively. However, implantation was not affected in 18 mg mancozeb treated mice with 4.92% pre-implantation loss when compared to oil treated controls. To study the temporal effect, the effective dose of 36 mg/kg body weight/d mancozeb was administered orally for 3 and 5 days and on day 3 only. There was a complete inhibition of implantation in 5 days treated mice with 100% pre-implantation loss and partial inhibition of implantation of 3 days treated mice with 75% pre-implantation loss. However, implantation was not affected in mice treated on day 3 only with 1.63% pre-implantation loss when compared to control mice. There was a significant decrease in the diestrus phase with the result there was a concomitant increase in the estrus phase and there was a significant decrease in the uterus weight with 24, 30 and 36 mg and for 3 and 5 days with 36 mg mancozeb treatment. Inhibition of implantation by mancozeb may be due to hormonal imbalance or its toxic effects.

Giardiasis in the adult population of Dakshina Kannada district of south India.

A 10-year study of Giardia intestinalis infections in adults was conducted in the Dakshina Kannada District of Karnataka State, South India. Coproparasitological investigations were made on 10,000 stool specimens collected from selected patients. The infection rate was 37.1% with a male disease rate of 91.4%. The highest incidence rate was recorded in the age group 15-26 years. A possible reason for this preponderance of giardiasis in these males, is discussed. The optimum time for transmission appeared to be during the warmer months of the year (May to October) which coincided with the greatest amount of rainfall. The most common symptoms were abdominal discomfort, flatulence, diarrhoea, weight loss and anorexia. The predominating symptoms in our series varied with socio-economic level. There appeared to be some correlation between the clinical manifestations and the immune status of the individual.

Extrarenal Wilms' tumor.

A rare case of retroperitoneal extrarenal Wilms' tumor with an unusual presentation is reported. The tumor contained a large cystic component and mimicked ascites clinically.