Collagen membrane functionalized with magnesium oxide via room-temperature atomic layer deposition promotes osteopromotive and antimicrobial properties.

Artificial bone grafting materials such as collagen are gaining interest due to the ease of production and implantation. However, collagen must be supplemented with additional coating materials for improved osteointegration. Here, we report room-temperature atomic layer deposition (ALD) of MgO, a novel method to coat collagen membranes with MgO. Characterization techniques such as X-ray photoelectron spectroscopy, Raman spectroscopy, and electron beam dispersion mapping confirm the chemical nature of the film. Scanning electron and atomic force microscopies show the surface topography and morphology of the collagen fibers were not altered during the ALD of MgO. Slow release of magnesium ions promotes bone growth, and we show the deposited MgO film leaches trace amounts of Mg when incubated in phosphate-buffered saline at 37 °C. The coated collagen membrane had a superhydrophilic surface immediately after the deposition of MgO. The film was not toxic to human cells and demonstrated antibacterial properties against bacterial biofilms. Furthermore, in vivo studies performed on calvaria rats showed MgO-coated membranes (200 and 500 ALD) elicit a higher inflammatory response, leading to an increase in angiogenesis and a greater bone formation, mainly for Col-MgO500, compared to uncoated collagen. Based on the characterization of the MgO film and in vitro and in vivo data, the MgO-coated collagen membranes are excellent candidates for guided bone regeneration.

Effect of Nano Ceramic Coating on Color Perceptibility and Acceptability of Polymethylmethacrylate: In Vitro and Clinical Study.

The effect of a novel nano-ceramic coating (TiO2) using an atomic layer deposition (ALD) technique on the surface of polymethyl methacrylate (PMMA) material was investigated. The patients' and clinicians' perception and acceptance of the PMMA color with TiO2 coating were also examined. In vitro color measurement was performed on thirty specimens (light, original, and dark pink) before and after TiO2 coating. Patients' and clinicians' perception and acceptance of color changes on PMMA were measured and compared. Descriptive and analytic statistics were analyzed (a = 0.05). TiO2 films were successfully deposited on the PMMA specimen by the ALD technique. Color changes after TiO2 coating were observed on all three PMMA shades, significantly higher than the established 50:50% perceptibility threshold, but below the established 50:50% acceptability threshold. The percentage of patients that perceived a color difference after TiO2 coating were 83.3%, 63.9%, and 77.8% for light, original, and dark pink, respectively. The percentages of clinicians that were satisfied with the color difference were 96.4%, 80%, and 69.2% for light, original, and dark pink, respectively. Color changes after TiO2 coating were observed, but below the acceptable threshold. The clinical survey demonstrated that a color difference was perceived but was clinically acceptable. In general, laypeople have lower perception and higher acceptance of changes in PMMA color than clinicians.

Is atomic layer deposition of silver possible on N95 masks?

Due to the COVID19 outbreak, there has been increasing interest in tailoring, modifying and improving conventional personal protective equipment to increase their service life and make them more effective against viruses and bacteria. Here, atomic layer deposition (ALD) was used to functionalize the filter of N95 mask with nano-islands of silver. X-ray photoelectron spectroscopy and x-ray absorption fine structure were used for ALD silver characterization; microbiological assay was conducted to study the effectiveness of the deposited silver against the air-borne pathogen Staphylococcus aureus (S. aureus). Results showed that silver ALD successfully functionalized the N95 mask at 90 and 120 °C for two different numbers of ALD cycles (1100 and 1500 cycles). The deposited silver nano-islands were stable on the N95 filter media against washing. The leaching of silver nano-islands was studied using inductively coupled plasma mass spectrometry of phosphate-buffered saline solution after soaking the mask in it over predetermined times. <9% of Ag was removed after a maximum time of 4 h that was investigated. Antimicrobial tests showed that for samples functionalized with 1100 ALD cycles of Ag, 76% reduction in S. aureus colony-forming units content was observed after 24 h of biofilm development on the mask surfaces.