Synaptophysin is a selective marker for axons in human cutaneous end organ complexes.

BACKGROUND: Small clear synaptic-like vesicles fill axon terminals of mechanoreceptors. Their functional significance is controversial and probably includes release of neurotransmitters from afferent axon terminals. Synaptophysin, a major protein of the synaptic vesicle membrane, is present in presynaptic endings of the central and peripheral nervous systems. It is also expressed in mechanosensory neurons which extend into skin forming sensory corpuscles. Nevertheless, synaptophysin occurrence in these structures has never been investigated. METHODS: Here we used immunohistochemistry to detect synaptophysin in adult human dorsal root ganglia, cutaneous Meissner and Pacinian corpuscles and Merkel cell-neurite complexes from foetal to elderly period. Moreover, we analyzed whether synaptophysin co-localizes with the mechano-gated protein PIEZO2. RESULTS: Synaptophysin immunoreactivity was observed in primary sensory neurons (36 ± 6%) covering the entire soma size ranges. Axons of Meissner's and Pacinian corpuscles were positive for synaptophysin from 36 and 12 weeks of estimated gestational age respectively, to 72 years old. Synaptophysin was also detected in Merkel cells (from 14 weeks of estimated gestational age to old age). Additionally in adult skin, synaptophysin and PIEZO2 co-localized in the axon of Meissner and Pacinian corpuscles, Merkel cells as well as in some axons of Merkel cell-neurite complexes. CONCLUSION: Present results demonstrate that a subpopulation of primary sensory neurons and their axon terminals forming cutaneous sensory corpuscles contain synaptophysin, a typical presynaptic vesicle protein. Although the functional relevance of these findings is unknown it might be related to neurotransmission mechanisms linked to mechanotransduction.

Prevalence of Premolars with Dens Evaginatus in a Taiwanese and Spanish Population and Related Complications of the Fracture of its Tubercle.

OBJECTIVE: Dens evaginatus (DE) is an odontogenic developmental anomaly that can be defined as a supernumerary tubercle structure that extends from the occlusal surface of the affected tooth. Tubercular fracture or attrition of the tubercle, invaded by pulp tissue, may cause various pulpal diseases, such as pulpitis, pulp necrosis, and periapical periodontitis. This study aimed to determine the prevalence of premolars with DE in Taiwanese and Spanish study populations and to report associated dental complications. METHODS: This study was based on the clinical examination of volunteers of Spanish and Taiwanese origin, recruited among the undergraduate dentistry students at CEU-Cardenal Herrera University, Valencia, Spain. Informed consents were obtained from students identified with DE. Additional examinations were performed, including vitality test, percussion, palpation, and radiographs, to diagnosis the status of the pulp and periapical tissue. RESULTS: The prevalence of DE among Taiwanese students was 4.08%, compared to 0% in the Spanish Caucasian group. In the Taiwanese group, the occurrence of DE in premolars was more common in the mandibular (78.9%) compared to the maxillar region (21.1%). In 84% of the cases, DE-affected teeth were found bilaterally. The mandibular second premolar was the most commonly involved tooth (57.8%). When investigating the complications related to DE among six affected Taiwanese students, it was found that two teeth (10.5%) had received pulp treatment, one of them prior to complete root formation. CONCLUSION: DE appears to be more prevalent in people of Mongoloid origin and rare in those of Caucasian origin. The wear or fracture of DE may affect pulpal tissue, leading to incomplete root growth.

Comparative study of root canals instrumented manually and mechanically, with and without Er:YAG laser.

OBJECTIVE: The aim of this study was to compare the scanning electron microscopic (SEM) morphology of root canal walls instrumented manually and mechanically with and without the application of Er:YAG laser. METHODS: Single-rooted, human, extracted teeth were cut at the cementoenamel junction. The teeth were divided into four groups: teeth that were simply instrumented manually, those that were instrumented manually and laser treated, a group that was mechanically instrumented, and a fourth in which the teeth were mechanically instrumented and laser treated. After instrumentation, the teeth were split longitudinally to facilitate their evaluation by SEM. RESULTS: The results show that the use of the Er:YAG laser is effective in removing the smear layer from root canal walls. CONCLUSIONS: The removal of the smear layer is essential to the success of endodontic treatment; the use of the Er:YAG laser combined with rotary and manual techniques improves the cleanliness of root canals.

The influence of a manual glide path on the separation rate of NiTi rotary instruments.

The dual purpose of this study was, to evaluate the fracture rate of Ni-Ti rotary instruments when following a manual glide path and using stainless steel hand files before carrying out instrumentation by means of rotary files and, to compare the results in this study with those obtained in two previous analyses, in which the glide path technique was not used. A total of 208 canals obtained from a pool of freshly extracted human mandibular and maxillary molars was divided into three groups corresponding to; K3, ProFile and ProTaper. The coronal 2/3 of each tooth were used. In all three groups the apical portion of the samples was prepared with size 10-20 stainless steel K-type hand files. The apical stops were prepared using K3, ProFile and ProTaper rotary instruments. Logistic regression model analysis indicated that breakage was significantly associated with the angle of curvature of the canal (OR = 1.078; 95% CI = 1.032-1.12; p = 0.001), and with the number of clinical uses (files used more than eight times broke more frequently that those used 1 or 2 times; OR: 22.686; 95% CI: 2.6-191.3; p = 0.004). The breakage rate obtained in the present study is significantly lower than in our previous studies, in which the angle of curvature was also greater than 30 degrees and rotational speed a constant 350 rpm, but in which the canals were not first prepared with hand files (25/205 = 12% versus 16/61 = 26%, p = 0.007). Based on the results of this study, we recommend the use of stainless steel hand files to prepare the apical 1/3 of curved canals before introducing rotary files.

Cephalometrics in children with Down's syndrome.

AIMS: To describe the craniofacial morphology of a group of patients with Down's syndrome using a cephalometric analysis of the lateral skull radiograph. MATERIALS AND METHODS: The studied sample consisted of 39 patients with Down's syndrome (24 boys, 15 girls) ranging from 7 to 18 years of age. The computerized cephalometric study of the lateral skull radiograph of each patient was carried out using the method described by Ricketts. RESULTS: Anterior cross-bite was observed in 38.4% of patients and diminished interincisal angle in 77%. Skeletal parameters matched the clinical norm, indicating mesofacial biotype, i.e., normal maxillomandibular growth. The lower incisors protruded in 84.6% of the individuals studied and were proinclined in 77%; upper incisors were protruded in 77% of the sample. The lower lip protruded in 84.6%. Analysis of craniofacial parameters showed average values within the clinical norm. Analysis of the inner cranium demonstrated normal inclination of the cranial base, while the length of the anterior skull base was diminished in 53.8%. CONCLUSIONS: From the skeletal perspective, patients with Down's syndrome who are in a period of growth demonstrate a reduction of the anterior skull base. From the dentoalveolar perspective, they show protrusion and proinclination of lower incisors, which is related to a tendency to anterior cross-bite and, to a lesser extent, to diminished overbite. Likewise, the lower lip protrusion observed in this study is related to the position of the lower incisor.