Synergistic interaction between hydrocolloids and kinnow peel biowaste for the development of edible fillers using response surface methodology.

Rapidly rising societal awareness about the planet sustainability through developing environmentally friendly and biodegradable alternatives to current impact of plastics waste represents an emerging need for establishing a circular bioeconomy of cleaner, safer, greener, and sustainable future. Until now, no investigation has been done on edible tableware made from leftover fruit peels. Presently, Kinnow mandarin is the most commercially farmed citrus fruit commodity, with the highest production, productivity, and popularity among all horticulture crops worldwide, generating vast quantity of peels ending up as putrefying biowaste that impacts ecosystem health. Sustainable efforts are therefore required in the circular economy to develop a creative and comprehensive strategy to address aforementioned issues, raising profitability, enhancing processing efficiency, and exploring "taste over waste," which contributes to overall sustainability. Therefore, in the current study, we established an approach for transforming the citrus peels biowaste into food-related end products by creating edible fillers, which is a sustainable material in terms of its functional, physical, and microbiological qualities for holding of confectionery products. The optimum level of independent variables with maximum desirability were found to be 0.75% calcium chloride concentration, 1.01% agar concentration, and 10% moisture content. A significant (p < 0.05) effect of process parameters was found in all responses. Model validation revealed that the model developed was accurate, with a prediction error ranging from - 9.96 to 3.28%. The technology developed for biowaste-based biofillers is still in a nascent stage, and it is expected that significant advancements will be made in the bio-refinery industries that can make edible fillers a reality in the future and might be helpful in contributing towards sustainable development. This research also demonstrated an efficient and novel approach towards a "zero-waste."

Development of yeast and microalgae consortium biofilm growth system for biofuel production.

Background: The current study aimed to develop a laboratory-scale biofilm photobioreactor system for biofuel production. Scope & Approach: During the investigation, Jute was discovered to be the best, cheap, hairy, open-pored supporting material for biofilm formation. Microalgae & yeast consortium was used in this study for biofilm formation. Conclusion: The study identified microalgae and yeast consortium as a promising choice and ideal partners for biofilm formation with the highest biomass yield (47.63 ± 0.93 g/m2), biomass productivity (4.39 ± 0.29 to 7.77 ± 0.05 g/m2/day) and lipid content (36%) over 28 days cultivation period, resulting in a more sustainable and environmentally benign fuel that could become a reality in the near future.

Enhancing the bio-prospective of microalgae by different light systems and photoperiods.

Microalgae are a source of highly valuable bioactive metabolites and a high-potential feedstock for environmentally friendly and sustainable biofuel production. Recent research has shown that microalgae benefit the environment using less water than conventional crops while increasing oxygen production and lowering CO2 emissions. Microalgae are an excellent source of value-added compounds, such as proteins, pigments, lipids, and polysaccharides, as well as a high-potential feedstock for environmentally friendly and sustainable biofuel production. Various factors, such as nutrient concentration, temperature, light, pH, and cultivation method, effect the biomass cultivation and accumulation of high-value-added compounds in microalgae. Among the aforementioned factors, light is a key and essential factor for microalgae growth. Since photoautotrophic microalgae rely on light to absorb energy and transform it into chemical energy, light has a significant impact on algal growth. During micro-algal culture, spectral quality may be tailored to improve biomass composition for use in downstream bio-refineries and boost production. The light regime, which includes changes in intensity and photoperiod, has an impact on the growth and metabolic composition of microalgae. In this review, we investigate the effects of red, blue, and UV light wavelengths, different photoperiod, and different lighting systems on micro-algal growth and their valuable compounds. It also focuses on different micro-algal growth, photosynthesis systems, cultivation methods, and current market shares.

A phyco-nanobionics biohybrid system for increased carotenoid accumulation in C. sorokiniana UUIND6.

Recent advancements in "phyco-nanobionics" have sparked considerable interest in the ability of microalgae to synthesize high-value natural bioactive compounds such as carotenoid pigments, which have been highlighted as an emergent and vital bioactive compound from both industrial and scientific perspectives. Such bioactive compounds are often synthesized by either altering the biogenetic processes existing in living microorganisms or using synthetic techniques derived from petroleum-based chemical sources. A bio-hybrid light-driven cell factory system was established herein by using harmful macroalgal bloom extract (HMBE) and efficient light-harvesting silver nanoparticles (AgNPs) to synthesize HMBE-AgNPs and integrating the synthesized HMBE-AgNPs in various concentrations (1, 2.5, 5 and 10 ppm) into the microalgae C. sorokiniana UUIND6 to improve the overall solar-to-chemical conversion efficiency in carotenoid pigment synthesis in microalgae. The current study findings found high biocompatibility of 5 ppm HMBE-AgNP concentration that can serve as a built-in photo-sensitizer and significantly improve ROS levels in microalgae (6.75 ± 0.25 µmol H2O2 g-1), thus elevating total photosynthesis resulting in a two-fold increase in carotenoids (457.5 ± 2.5 µg mL-1) over the native microalgae without compromising biomass yield. NMR spectroscopy was additionally applied to acquire a better understanding of pure carotenoids derived from microalgae, which indicated similar peaks in both spectra when compared to ß-carotene. Thus, this well-planned bio-hybrid system offers a potential option for the cost-effective and long-term supply of these natural carotenoid bio-products.

Heavy metal tolerance in microalgae: Detoxification mechanisms and applications.

The proficiency of microalgae to resist heavy metals has potential to be beneficial in resolving various environmental challenges. Global situations such as the need for cost-effective and ecological ways of remediation of contaminated water and for the development of bioenergy sources could employ microalgae. In a medium with the presence of heavy metals, microalgae utilize different mechanisms to uptake the metal and further detoxify it. Biosorption and the next process of bioaccumulation are two such major steps and they also include the assistance of different transporters at different stages of heavy metal tolerance. This capability has also proved to be efficient in eradicating many heavy metals like Chromium, Copper, Lead, Arsenic, Mercury, Nickel and Cadmium from the environment they are present in. This indicates the possibility of the application of microalgae as a biological way of remediating contaminated water. Heavy metal resistance quality also allows various microalgal species to contribute in the generation of biofuels like biodiesel and biohydrogen. Many research works have also explored the capacity of microalgae in nanotechnology for the formation of nanoparticles due to its relevant characteristics. Various studies have also revealed that biochar deduced from microalgae or a combination of biochar and microalgae can have wide applications specially in deprivation of heavy metals from an environment. This review focuses on the strategies adopted by microalgae, various transporters involved in the process of tolerating heavy metals and the applications where microalgae can participate owing to its ability to resist metals.

Impact of irradiation on physico-chemical and nutritional properties of fruits and vegetables: A mini review.

Background: Fruits and vegetables are healthy because they contain good nutrients and secondary metabolites that keep the body healthy and disease-free. Post-harvest losses of fresh fruits and vegetables limit access and availability as a result of foodborne infections and poor storage technologies. The selection of fruits and vegetables depend on the starting microbial load, the size of fruits and vegetables, and the type of infrastructure. Scope and approach: Despite the positive impacts of conventional thermal (roasting, boiling, blanching) and some non-thermal processing techniques such as High Pressure Processing (HPP), Pulse Electric Field (PEF), Cold Plasma Technology (CPT) on shelf-life extension, their use is commonly associated with a number of negative consequences on product quality such as cold plasma treatment increases the acidity and rate of lipid oxidation and further decrease the colour intensity and firmness of products. Similarly, in high pressure processing and pulse electric field there is no spore inactivation and they further limit their application to semi-moist and liquid foods. On that account, food irradiation, a non-thermal technique, is currently being used for post-harvest preservation, which could be very useful in retaining the keeping quality of various fresh and dehydrated products without negatively affecting their versatility and physico-chemical, nutritional and sensory properties. Conclusion: Existing studies have communicated the effective influence of irradiation technology on nutritional, sensory, and physico-chemical properties of multiple fruits and vegetables accompanying consequential deduction in microbial load throughout the storage period. Food irradiation can be recognized as a prevalent, safe and promising technology however, still is not fully exploited on a magnified scale. The consumer acceptance of processed products has always been a significant challenge for innovative food processing technologies such as food irradiation. Therefore, owing to current review, additional scientific evidences and efforts are still demanded for increasing its technological request.

Edible hydrocolloids as sustainable substitute for non-biodegradable materials.

Edible hydrocolloid polymers have created significant deliberation in modern eons due to their numerous advantages of being used as edible materials over synthetic materials, which could be helpful to the food industry as well as toward environmental sustainability. In the current scenario, where biopolymers have replaced petroleum-based materials, natural edible hydrocolloids are now in demand to combat the harmful impacts of non-biodegradable materials. This review addresses the importance of natural edible hydrocolloids, materials that can be used to form hydrocolloid gel, their properties, synergistic interactions of hydrocolloids and various applications in food and biomedical fields.

Cold plasma technology: advanced and sustainable approach for wastewater treatment.

Cold plasma has been a potent energy-efficient and eco-friendly advanced oxidation technology which has gained attention in recent decades as a non-thermal approach in diverse forms of applications. This review highlights a comprehensive account of the implementation of this technology in the field of wastewater treatment to resolve certain issues regarding the degradation of numerous aqueous pollutants and water-borne pathogenic microorganisms including viruses up to a significant level. The paper addresses plasma chemistry sources and mechanisms on wastewater treatment and impact on various physical, chemical, and biological characteristics of treated water. Furthermore, studies have revealed that this emerging technology is effective in inactivating SARS-CoV-2 or coronavirus, which serves as a transmission channel for this lethal virus in wastewater. Despite these benefits, the development of cold plasma as a wastewater treatment technique is still hampered by a lack of information like capital investment, proficient application, liveability, and operating cost, thus necessitating additional research for its booming commercialization, as this can be an emerging approach to solving water crises and meeting the demand for fresh or potable water resources.

The potential of nuclear magnetic resonance (NMR) in metabolomics and lipidomics of microalgae- a review.

Microalgae biotechnology has made it possible to derive secondary bioactive metabolites from microalgae strains that have opened up their entire potential to uncover a wide range of novel metabolic capabilities and turn these into bio-products for the development of sustainable bio-refineries. Nuclear Magnetic Resonance Technology (NMR) has been one of the most successful and functional research technology over the past two decades to analyse the composition, structure and functionality of distinct metabolites in the different microalgae strains. This technology offers qualitative as well as quantitative knowledge about the endogenous metabolites and lipids of low molecular mass to offer a good picture of the physiological state of biological samples in metabolomics and lipidomics studies. Henceforth, this review is aimed at introducing the metabolomics and lipidomics studies into the field of NMR technology and also highlights the protocols for the isolation and metabolic measurements of metabolites from microalgae that should be redirected to resource recovery and value-added products with a systematic and holistic approach for scalability or sustainability.