RESUMO
The effects of co-administration of a cassava rich diet and alcohol in rats were investigated. The animals were divided into four groups (1) Control, (2) Alcohol, (3) Cassava and (4) Alcohol + Cassava. Consumption of alcohol along with cassava reduced the alcohol induced toxicity which was evidenced by the lower activities of GOT, GPT, GGT, acid phosphatase and alkaline phosphatase in the liver and serum of co-administered group. The pyruvate content in the blood increased while the lactate content, lactate/pyruvate ratio and the activity of LDH decreased in the blood due to co-administration. The blood cyanide content, serum thiocyanate content and the activities of rhodanase and beta-glucuronidase increased on co-administration. The histopathological studies also revealed that co-administration reduced the alcohol induced toxicity.
Assuntos
Dieta , Etanol/farmacologia , Manihot , Fosfatase Ácida/sangue , Alanina Transaminase/sangue , Fosfatase Alcalina/sangue , Animais , Aspartato Aminotransferases/sangue , Cianetos/análise , Cianetos/sangue , Glucuronidase/sangue , L-Lactato Desidrogenase/sangue , Ácido Láctico/sangue , Masculino , Manihot/química , Ácido Pirúvico/sangue , Ratos , Ratos Sprague-Dawley , Tiocianatos/sangue , gama-Glutamiltransferase/sangueRESUMO
The effects of feeding a cassava (Manihot esculenta) rich diet on alcohol induced peroxidative damages were investigated in male albino rats. Rats were divided into four groups and maintained for 60 days as follows. (1) CONTROL GROUP: cassava free diet, (2) alcohol group: cassava free diet+ethanol (4 g/kg body wt/day), (3) cassava group: cassava diet and (4) alcohol+cassava group: cassava diet+ethanol (4 g/kg body wt/day). Results revealed that alcohol induced significant lipid peroxidation, since the lipid peroxidation products malondialdehyde (MDA), hydroperoxides and conjugated dienes were elevated in the liver. The activities of free radical scavenging enzymes such as superoxide dismutase (SOD), catalase and glutathione reductase were reduced and glutathione content was decreased in the liver. But the co-administration of a cassava rich diet increased the activity of free radical scavenging enzymes and glutathione content. The level of lipid peroxides in the liver was also decreased on co-administration of cassava. But the oxidative damage caused by cassava was potentiated by alcohol administration. These studies suggested that consumption of alcohol along with cassava offered some protection against the alcohol induced oxidative stress. So we isolated the cyanoglycoside rich fraction from cassava and its impact on rats administered alcohol was also investigated. The results revealed that alcohol induced oxidative stress was potentiated by the co-administration of cyanoglycoside rich fraction. These studies suggested that the fiber and antioxidant vitamins present in the cassava may be playing a protective role against the alcohol induced oxidative stress.
Assuntos
Etanol/farmacologia , Glicosídeos/farmacologia , Peroxidação de Lipídeos/efeitos dos fármacos , Manihot/metabolismo , Estresse Oxidativo/efeitos dos fármacos , Extratos Vegetais/farmacologia , Animais , Antioxidantes/farmacologia , Depressores do Sistema Nervoso Central/farmacologia , Interações Medicamentosas , Peróxidos Lipídicos/metabolismo , Fígado/efeitos dos fármacos , Fígado/metabolismo , Masculino , Malondialdeído/metabolismo , Manihot/química , Ratos , Ratos Sprague-DawleyRESUMO
The effect of the administration of blackgram fiber (Phaseolus mungo) on the metabolism of carbohydrates was studied in rats fed 30% NDF (neutral detergent fiber) diet. The experimental group showed a significant increase in liver glycogen level and a significant decrease in blood glucose. Significant increases in the activities of glycogen phosphorylase, hexokinase, fructose-1, 6-diphosphatase, glucose-6-phosphate dehydrogenase and isocitrate dehydrogenase were observed in the experimental group. The activities of phosphoglucomutase and glucose-6-phosphatase were significantly lower in rats fed the fiber diet. The study showed that blackgram fiber exhibits significant hypoglycemic action in experimental animals.
Assuntos
Metabolismo dos Carboidratos , Fibras na Dieta/metabolismo , Hipoglicemiantes/metabolismo , Fígado/enzimologia , Phaseolus , Animais , Glicemia/análise , Detergentes , Fibras na Dieta/administração & dosagem , Índice Glicêmico , Hipoglicemiantes/administração & dosagem , Fígado/metabolismo , Glicogênio Hepático/análise , Masculino , Phaseolus/química , Distribuição Aleatória , Ratos , Ratos Sprague-DawleyRESUMO
Feeding 50% ethanolic extract of A. aspera to male rats resulted in reduced sperm counts, weight of epididymis, serum level of testosterone and testicular activity of 3beta-hydroxysteroid dehydrogenase, while motility of the sperm and activity of the HMG CoA reductase were not affected. Cholesterol level in the testis, incorporation of labelled acetate into cholesterol, 17-ketosteroids in urine and hepatic and fecal bile acids were increased. The results suggest that ethanolic extract of A. aspera caused reproductive toxicity in male rats and the action may be by suppressing the synthesis of androgen.