[application of the analytical transmission electron microscopy techniques for detection, identification and visualization of localization of nanoparticles of titanium and cerium oxides in mammalian cells].

This work represents the results of the study on applicability of the modern methods of analytical transmission electron microscopy for detection, identification and visualization of localization of nanoparticles of titanium and cerium oxides in A549 cell, human lung adenocarcinoma cell line. A comparative analysis of images of the nanoparticles in the cells obtained in the bright field mode of transmission electron microscopy, under dark-field scanning transmission electron microscopy and high-angle annular dark field scanning transmission electron was performed. For identification of nanoparticles in the cells the analytical techniques, energy-dispersive X-ray spectroscopy and electron energy loss spectroscopy, were compared when used in the mode of obtaining energy spectrum from different particles and element mapping. It was shown that the method for electron tomography is applicable to confirm that nanoparticles are localized in the sample but not coated by contamination. The possibilities and fields of utilizing different techniques for analytical transmission electron microscopy for detection, visualization and identification of nanoparticles in the biological samples are discussed.

[Follow-up results of surgical correction of ocular ischemic syndrome]. / Otdalennye rezul'taty khirugicheskoi korrektsii glaznogo ishemicheskogo sindroma.

The influence of surgeries in the carotid arteries produced on the visual functions and ocular blood circulation was studied in patients with ocular ischemic syndrome (OIS) during the remote postoperative period. A total of 180 patients with OIS (including 104 patients with an acute OIS clinical course and 76 patients with primary chronic clinical course) and with a pronounced stenosis of the carotid arteries were examined preoperatively and postoperatively within 1 or 2 years. A reliably improved visual acuity (preoperatively -0.37 +/- 0.05; and postoperatively -0.52 +/- 0.07; p < 0.01), positive dynamics in the electric sensitivity threshold and in a lability of the optic nerve were observed in patients with the acute OIS clinical course after reconstructive surgeries in the carotid arteries. An increase in contrast sensitivity of the visual analyzer was detected in 28.8% of patients with the acute clinical course and in 10.5% of patients with the chronic OIS clinical course. An improved blood circulation through the ocular artery was stated in patients of both groups. Reconstructive surgeries in the carotid arteries are most effective in correcting the acute OIS clinical variation.

[Clinical picture of open-angle glaucoma in patients with critical stenosis of the internal carotid artery]. / Osobennosti kliniki otkrytougol'noi glaukomy u patsientov s kriticheskim stenozom vnutrennei sonnoi arterii.

Clinical picture of primary open-angle glaucoma (POAG) was studied in 32 patients (57 eyes) with pronounced stenosis of the extracranial segment of the internal carotid artery (ICA) on the side of the glaucomatous eye. Major clinical signs were detected: conjunctival angiopathy, pronounced diffuse dystrophy of the iris, absence of pigmented iridal rim, pseudoexfoliations, pale optic disk with saucer-like excavation of different severity. Intraocular pressure (IOP) was 26-34 mm Hg and could not be normalized by drug therapy or antiglaucomatous surgery. Ultrasonic methods for examination of the eyeball vessels showed pronounced decrease of the bloodflow velocity and a significant increase of resistance index in the orbital and central retinal arteries. Reconstructive operations on the carotid arteries performed in POAG patients for critical stenosis of ICA improved the eyeball hemoperfusion and decreased IOP.

[Doppler ultrasonography of penile vessels in combination with various methods of erectile stimulation in diagnosis of erectile dysfunction]. / Ul'trazvukovaia dopplerografiia sosudov polovogo chlena v sochetanii s razlichnymi sposobami stimuliatsii érektsii v diagnostike érektil'noi disfunktsii.

Ultrasonic dopplerographic investigation (Ultramark-9, USA) was made in 227 males. 50 males with normal erection served controls. Stimulation of erection with an individual LOD-device is a low-invasive, convenient to standardize technique in ultrasonic dopplerographic examination of penile vessels for diagnosis of erectile dysfunction. Erection stimulation by different methods in the same patient during penile vessel dopplerography raises diagnostic value of this method.

[Blood flow in ocular vessels in 2 types of ocular ischemic syndrome]. / Krovotok v sosudakh glaza pri dvukh tipakh techeniia glaznogo ishemicheskogo sindroma.

Ultrasonic triplex scanning of the orbit (gray scale two-dimensional ultrasonic examination, color Doppler mapping (CDM), and dopplerography) was carried out in 148 patients with ocular ischemic syndrome (OIS) aged 35-70 years, 104 of these with acute and 44 with primary chronic course. Signs of disordered bloodflow in the orbital and central retinal arteries were detected in patients with acute disease: 2-fold decreased maximum systolic bloodflow velocity, 5-fold decreased end diastolic bloodflow velocity, and 1.5 times increased resistance index in comparison with the norm. Chronic course of OIS was characterized by decreased maximum systolic velocity (by 1.5 times) and end diastolic bloodflow velocity (3-fold) in comparison with the norm in both arteries. Studies of ocular bloodflow by CDM timely detected angiological disorders in OIS and help define (in cooperation with angiosurgeon) the indications for angioreconstructive operations.