Broiler short-term feed preferences measured with SRAbox, a new feed choice procedure.

Three dietary compositions and 3 methods of pellet preparation were used in different choice situations to improve our understanding of the factors perceived by broiler chickens in the physical structure of their diet. Within the same visual field, broiler chickens were offered 8 microfeeders containing different pelleted feeds that could be reached from the same position of the bird. Chickens were daily tested during 5-min tests following a 45-min period of feed withdrawal, after adaptation to individual cages and experimental feeders. Two feeds (4 microfeeders/feed in alternate positions) were weighed prior to and after the test. Behavior of the chickens was videotaped during the test to count the number of pecks given to each microfeeder. In a first experiment, 2 feeds of distinct composition were either steam or cold-pelleted. The tests of choice were repeated daily from 22 to 25 and from 35 to 38 d of age on 96 male broiler chickens. Significant preferences were measured from the first test. Similar preferences were measured when chickens received the same choice or alternating choices from one test to another. Differences of feed intake between 2 feeds during 5 min were significantly correlated to differences of number of pecks (R2 = 0.85). In most cases, preferences were established within the first minute of pecking. Steam pellets were significantly preferred to cold pellets for one feed composition but not for the other. Preferences for compositions were consistent whatever the pellet preparation and probably associated with the color of the feeds. A second experiment confirmed that 38-d-old broiler chickens seemed to prefer light colors. Short-term preferences of a chicken for a feed were rather influenced by physical characteristics of feed particles (size, color, hardness) than by the origin of raw materials. Feed pecking may change because of feed composition itself, because of its consequences on color or texture cues, or both.

Early-age thermal conditioning reduces uncoupling protein messenger RNA expression in pectoral muscle of broiler chicks at seven days of age.

Early-age thermal conditioning (TC) by exposing young chicks to 40 C for 24 h reduces body temperature (Tb) and has been showed by others to improve long-term resistance of broilers to heat stress. Uncoupling oxidative phosphorylation in pectoral muscle mitochondria might be related to heat production. Fertile eggs were hatched under video control, and 161 pedigree chicks froml2 sires and 22 dams were immediately allocated to two groups (T, a group composed of 81 chicks exposed to TC at 5 d of age, and N, a control group of 80 nonexposed chicks). Body weights and Tb were measured at 2 and 7 d of age. Five pairs (one N and one T) of full sib chicks from families that exhibited the largest difference of Tb variation from 2 to 7 d of age between the two treatments were chosen for pectoral muscle sampling. Avian uncoupling protein (avUCP) messenger RNA expression was measured by reverse transcript-PCR coupled to southern blot in the pectoral muscle of 7-d-old broiler chicks. At 7 d of age, there were no BW differences between treatments and Tb was significantly reduced by TC (-0.13 C on average). Heritability of Tb variation between 2 and 7 d was 0.38 +/- 0.20 (SE) for T chicks and 0.35 +/- 0.17 for N chicks without a significant genetic correlation between the two environments. Expression of avUCP mRNA was significantly (85%) lower in T chicks than in N chicks. Uncoupling protein mRNA expression in pectoral muscle and Tb are quickly adjusted in broiler chicks 24 h after early thermal conditioning.

A simple method for testing odor detection and discrimination in chicks.

A simple procedure for testing newly hatched chicks' responses to olfactory stimuli is described. Chicks that were hand held under a heatlamp became inactive and closed their eyes (as if asleep) within 2 min. Sleeping chicks displayed overt behavioral responses to an odorized q-tip (head shaking, beak clapping) that were not observed in awake chicks. Sleeping latencies were shorter for 1-day-old chicks that had been food deprived prior to testing than for chicks that had ad lib access to food and water. When airborne odors were presented by squeezing a soft plastic odor bottle near the beak, sleeping chicks' reactions to mint were of a greater magnitude than their responses to lavender or orange scents. Nonetheless, all three odors elicited more pronounced behavioral responses than did the water control stimulus. This method allows rapid testing of individual chicks for odor detection and discrimination.

Supernumerary ring chromosomes containing chromosome 17 sequences. A specific feature of dermatofibrosarcoma protuberans?

Mystery surrounds the mechanisms by which the uncommon cutaneous tumor dermatofibrosarcoma protuberans (DP) arises and progresses. A clue may be at hand in the form of extra abnormal chromosomes (one of three ring chromosomes per case with or without other chromosome abnormalities) seen in some 10 cases, including five cases in our experience. The specificity of the rings to DP would be enhanced if the rings were found to contain a contribution from a constant chromosome. We here report fluorescence in situ hybridization (FISH) results bearing on the chromosomal content of DP rings, together with clinical, pathologic, and cytogenetic documentation of our first two cases, which were briefly reported earlier, and three new DP cases. To dissect a translocation (in our 2nd case), we probed by FISH and discovered chromosome 17 sequences in the rings in all five DP cases. Nonfluorescent bands were seen on some rings painted with a whole chromosome 17 probe, indicating the presence in these rings of foreign chromosome sequences. The complexity of the rings was underscored by the detection in only one case of chromosome 17 centromeric sequences. The situation in DP seems to have parallels to that in well-differentiated liposarcoma, another tumor of intermediate malignancy with extra abnormal marker chromosome containing contributions from a constant chromosome and variable donors. In DP the supernumerary rings are clearly specific and significant.