Groundwater quality and hydrochemical characteristics in the upper Seybouse sub-basin, Northeast Algeria.

The present study aims to evaluate the groundwater quality in an area characterized by significant human anthropic activities within the upper Seybouse. In order to assess the quality, a total of 20 samples were analyzed to identify the chemical and bacteriological composition of the water, its variations, and their potential impacts on the environment and human health. The results revealed concentrations of the chemical and bacteriological elements exceeding the WHO standards, with high levels of electrical conductivity (EC) (peak = 4210 µS/cm), Ca2+ (peak = 340.68 mg/L), Na+ (peak = 360 mg/L), HCO3- (peak = 287 mg/L), Cl- (peak = 542 mg/L), SO42- (peak = 687 mg/L), NO3- (pek = 65.91 mg/L), fecal coliforms (FC) (peak = 160 UFC/mL), fecal Streptococcus (FS) (peak = 43 UFC/mL), and Clostridium perfringens (CP) (peak = 29 UFC/mL). Within the basin, two different facies have been identified: Cl-SO4-Na type and Cl-SO4-Ca type. The calculated Water Quality Index (WQI) indicates that none of the groundwater samples are suitable for drinking or human consumption. The detection of pathogenic microorganisms through diverse molecular methods has revealed the existence of eight distinct species, encompassing pathogenic strains that can affect human health. Moreover, the dissolution of geologic formations can influence the water's chemistry. In this region, groundwater pollution seems to be influenced by anthropogenic and agricultural factors such as fertilizer application, irrigation practices, and the release of domestic sewage.

Alternative Approaches to Feeding Small Ruminants and Their Potential Benefits.

Small ruminants, such as sheep (Ovisaries) and goats (Capra hircus), contribute to approximately 475 million metric tons of carbon dioxide equivalent (MtCO2e) greenhouse gas (GHG) emissions, accounting for approximately 6.5% of the global emissions in the agriculture sector. Crop residues, silage, grasses, hay, browse, plant leaves, shrubs, agro-industrial by-products, poultry litter, and other alternative feed sources are frequently utilized for small ruminant production. The use of these valuable alternative feeds can significantly improve animal productivity and reduce carbon footprints and GHG fluxes, making it both environmentally friendly and cost-effective. Additionally, these alternative feeds possess antioxidant, antimicrobial, and antiseptic properties that can enhance the quality of the meat and milk produced. By impacting the bacteria involved in ruminal biohydrogenation, alternative feeds can reduce methane emissions and contribute to a decrease in the carbon footprint. Overall, the use of alternative feed sources for small ruminants generally improves their apparent nutrient digestibility and productivity, and has an impact on the production of greenhouse gases, especially methane. Finally, this review recommends evaluating the economic analysis of reducing methane emissions in small ruminants by utilizing different feed sources and feeding techniques.

Trace elements in tea in Ouargla, Algeria and health risk assessment.

Tea is one of the most common drinks, consumed for its pleasant flavour and several medicinal values. The present study aimed to determine the levels of trace elements in tea products marketed in the Saharan region of Ouargla, Algeria and to evaluate the health risks associated with its regular consumption in adults and infants. To this aim, 78 tea samples were analysed by Inductively Coupled Plasma - Mass Spectrometry. Tea samples appeared to be contaminated by lead (0.73 ± 0.08 µg g-1) and aluminium (0.22 ± 0.02 µg g-1). Cadmium, arsenic, mercury, cobalt, manganese, nickel, chromium, zinc and copper were also detected. General linear model analysis indicated that black tea samples were the most contaminated. Tea samples packed in tea bags were the most contaminated with arsenic, aluminium and manganese. The hazard index was 0.28 and 1.33 for adults and for infants, respectively, indicating adverse non-carcinogenic effects in infants.

Evaluation of the adulteration of camel milk by non-camel milk using multispectral image, fluorescence and infrared spectroscopy.

In the present study, the focus was to evaluate the potential of three spectroscopic techniques (Mid Infrared -MIR-, fluorescence, and multispectral imaging -MSI-) to check the level of adulteration in camel milk with goat, cow, and ewe milks. Camel milk was adulterated with goat, ewe, and cow milks, respectively, at 6 different levels viz. 0.5, 1, 2, 5, 10, and 15%. After preprocessing the data with standard normal variate (SNV), multiplicative scattering correction (MSC), and normalization (area under spectrum = 1), partial least squares regression (PLSR) and partial least squares discriminant analysis (PLSDA) were used to predict the adulteration level and their belonging group, respectively. The PLSR and PLSDA models, validated using external data, highlighted that fluorescence spectroscopy was the most accurate technique giving a R2p ranging between 0.63 and 0.96 and an accuracy ranging between 67 and 83%. However, no technique has allowed the construction of robust PLSR and PLSDA models for the simultaneous prediction of contamination of camel milk by the three milks.

Determination of Heavy Metal Levels and Health Risk Assessment of Raw Cow Milk in Guelma Region, Algeria.

During the recent decades, adverse effects of unexpected contaminants, such as heavy metals on raw cow milk quality, have threatened human health. The objective of this study was to determine heavy metal levels in raw milk collected from autochthonous bovine breeds in the eastern region of Algeria. Eighty-eight pooled milk samples were analyzed using atomic absorption spectrometry for Pb, Cd, Cr, Cu, Ni, Fe, and Zn, and dietary risks were estimated for infants, children, and adults with minimum, average, and maximum milk consumption scenarios. Results revealed that Pb (0.94 ± 0.49 mg/kg), Cd (0.03 ± 0.01 mg/kg), and Cu (0.14 ± 0.08 mg/kg) levels in all analyzed samples were higher than their corresponding maximum residue levels (MRLs). The task hazard quotient (THQ) values suggest potential risk for infants in the three scenarios from Pb, Cd, and Cr; for children in the three scenarios from Pb and in the high scenario from Cr; and for adults in the medium and high scenarios from Pb. The hazard index (HI) values were higher than 1, and the contributions of each metal to the overall HI followed a descending order of Pb, Cr, Cd, Ni, Zn, Cu, and Fe with values of 68.19%, 15.39%, 6.91%, 4.94%, 3.42%, 0.88%, and 0.28%, respectively. Our results indicated that there may be a potential risk of heavy metals, especially Pb, for infants through raw cow milk consumption. Moreover, data actualization and continuous monitoring are necessary and recommended to evaluate heavy metal effects in future studies.

Relationship between endocrine disruptors and obesity with a focus on bisphenol A: a narrative review.

Introduction: Scientific data suggest that early exposure to endocrine-disrupting chemicals (EDCs) affect -repro, -neuro, -metabolic systems, to which are added other notions such as mixtures, window and duration of exposure, trans-generational effects, and epigenetic mechanisms. Methods: In the present narrative review, we studied the relationship between exposure to EDCs with the appearance and development of obesity. Results: Exposure to EDCs like Bisphenol A during the early stages of development has been shown to lead to weight gain and obesity. EDCs can interfere with endocrine signaling, affect adipocytes differentiation and endocrine function and disrupt metabolic processes, especially if exposure occurs at very low doses, in the mixture, during early development stages for several generations. Conclusion: Exposure to EDCs is positively associated with obesity development. Moreover, the use of integrative approaches which mimicking environmental conditions are necessary and recommended to evaluate EDCs' effects in future studies.

Use of multivariate analysis as a tool in the morphological characterization of the main indigenous bovine ecotypes in northeastern Algeria.

Sustainability in livestock farming requires monitoring of autochthonous breeds which are well adapted to the local environment. The morphometric measurements seem to be the first approach which can provide useful information on the suitability of animal genetic resources for selection. In this work, thirteen morphometric variables were used for the phenotypic characterization of 130 adult autochthones cattle randomly selected from 30 local farms in Guelma. There were cases from four commonly accepted and traditional ecotypes: Guelmois, Cheurfa, Sétifien and Fawn. The results showed several and significant positive correlations between the different variables. Correlations were analyzed using Varimax orthogonal rotation PCA and three factors were extracted, which explain more than 75% of the total variation in the four ecotypes. Stepwise discriminant analysis showed that 6 of the 13 variables had discriminatory power to define the phenotypic profile of the ecotypes. Canonical discriminant analysis indicated that the Sétifien ecotype is separate from the other three ecotypes. Mahalanobis distances were significant between the different ecotypes except for the distance between the Guelmois and Fawn ecotypes. The cross-validation procedure assigned 91.42% of the Sétifien animals to their genetic group, while the percentages of animals assigned to the Cheurfa, Guelmois and Fawn ecotypes were 80.00%, 65.71% and 53.33% respectively. The multivariate approach has proven to be effective in differentiating the four ecotypes, with clear morphological differences from the Sétifien ecotype that may benefit from a genetic improvement program for more sustainable genetic resources preservation.

Heavy metals levels in raw cow milk and health risk assessment across the globe: A systematic review.

This systematic review presents the potential toxicity of heavy metals such as lead (Pb), mercury (Hg), cadmium (Cd), iron (Fe), nickel (Ni), aluminum (Al), and copper (Cu) in raw cow milk, focusing on their contamination sources and on the assessment of the related human health risk. Multiple keywords such as "raw cow milk, heavy metals, and human health" were used to search in related databases. A total of 60 original articles published since 2010 reporting the levels of these metals in raw cow's milk across the world were reviewed. Data showed that the highest levels of Ni (833 mg/L), Pb (60 mg/L), Cu (36 mg/L) were noticed in raw cow milk collected in area consists of granites and granite gneisses in India, while the highest level of Cd (12 mg/L) was reported in barite mining area in India. Fe values in raw cow milk samples were above the WHO maximum limit (0.37 mg/L) with highest values (37.02 mg/L) recorded in India. The highest Al level was (22.50 mg/L) reported for raw cow's milk collected close to food producing plants region in Turkey. The Target Hazard Quotients (THQ) values of Hg were below 1 suggesting that milk consumers are not at a non-carcinogenic risk except in Faisalabad province (Pakistan) where THQ values = 7.7. For the other heavy metals, the THQ values were >1 for Pb (10 regions out of 70), for Cd (6 regions out of 59), for Ni (3 out of 29), and for Cu (3 out of 54). Exposure to heavy metals is positively associated with diseases developments. Moreover, data actualization and continuous monitoring are necessary and recommended to evaluate heavy metals effects in future studies.

The Effect of Mixing Milk of Different Species on Chemical, Physicochemical, and Sensory Features of Cheeses: A Review.

The yield and quality of cheese are associated with the composition, physicochemical, sensory, rheological, and microbiological properties of milk and with the technology applied to the milk before and/or during cheese processing. This review describes the most important research on cheeses obtained from processing mixtures of different milk species and discusses the effect of milk mixtures (i.e., species and mixture ratios) on composition, physicochemical, sensory, rheological, and microbiological properties of cheeses. More specifically, the present review paper will gather and focus only on studies that have provided a clear comparison between cheeses produced from a mixture of two milk species to cheeses produced from only one species.

Male rat exposure to low dose of di(2-ethylhexyl) phthalate during pre-pubertal, pubertal and post-pubertal periods: Impact on sperm count, gonad histology and testosterone secretion.

Di (2-ethylhexyl) phthalate (DEHP) is the most ubiquitous endocrine disruptor in the environment. The present study aimed to investigate the low dose effects of DEHP on the male reproductive system of rats exposed during the pre-pubertal, pubertal and post-pubertal periods. Male Wistar rats were daily gavaged by DEHP from postnatal day (PND) 21 to PND 120 with 0.5, 50 and 5000µg/kgBW/d. A decrease in sperm count of 41%, 24% and 46% was observed at 0.5, 50 and 5000µg/kgBW/d respectively. A decrease of Sertoli cells number was observed at 50 and 5000µg/kgBW/d (22% and 42%, respectively). Non-monotonic dose-response was observed for testosterone levels with a significant increase at 50µg/kgBW/d associated to a notable enhancement of Leydig cells number (35%). In conclusion, our results showed that postnatal exposure to low doses of DEHP affects sperm count, Sertoli and Leydig cells number and testosterone level.

Early endocrine disruptors exposure acts on 3T3-L1 differentiation and endocrine activity.

Introduction: Data from last years suggested that early exposure to endocrine disruptors (EDs) can predispose newborns to endocrine dysfunction of adipocytes, obesity, and associated disorders. The implication of EDs at low doses on adipocyte development has been poorly investigated. For instance, vinclozolin (V) is a dicarboximide fungicide widely used in agriculture since the 90's, alone or in mixture with genistein (G), an isoflavonoid from Leguminosae. This study aims to identify the effect of vinclozolin alone or with genistein, on adipose tissue properties using cell culture. Methods: In steroid-free conditions, 3T3-L1 pre-adipocytes were induced to differentiate in the presence of EDs, singularly or in mixtures, for 2 days. DNA and triglyceride (TG) levels were measured on days 0, 2 and 8 of differentiation. Leptin secretion was measured only on the eighth day. Results: We show that low doses of G (25 µM) and V (0.1 µM) inhibit pre-adipocytes differentiation. This inhibition has been represented by a decreasing in DNA content (µg/well) and decreasing in TG accumulation (mg/mL) in 3T3-L1 cells. Nevertheless, V increased the anti-adipogenic properties of G. Conclusion: This study confirms that EDs singularly or in mixtures, introduced during early stages of life, could affect the differentiation and the endocrine activity of adipocytes, and can act as potential factors for obesity.

Enamel hypomineralization due to endocrine disruptors.

There has been increasing concerns over last 20 years about the potential adverse effects of endocrine disruptors (EDs). Bisphenol A (BPA), genistein (G) and vinclozolin (V) are three widely used EDs having similar effects. Tooth enamel has recently been found to be an additional target of BPA that may be a causal agent of molar incisor hypomineralization (MIH). However, populations are exposed to many diverse EDs simultaneously. The purpose of this study was therefore to assess the effects of the combination of G, V and BPA on tooth enamel. Rats were exposed daily in utero and after birth to low doses of EDs mimicking human exposure during the critical fetal and suckling periods when amelogenesis takes place. The proportion of rats presenting opaque areas of enamel hypomineralization was higher when rats were treated with BPA alone than with a combination of EDs. The levels of mRNAs encoding the main enamel proteins varied with BPA treatment alone and did not differ significantly between controls and combined treatment groups. In vitro, rat ameloblastic HAT-7 cells were treated with the three EDs. BPA induced enamelin and reduced klk4 expression, G had no such effects and V reduced enamelin expression. These findings suggest that combinations of EDs may affect enamel less severely than BPA alone, and indicate that enamel hypomineralization may differ according to the characteristics of the ED exposure.

A multi-generational study on low-dose BPA exposure in Wistar rats: effects on maternal behavior, flavor intake and development.

Bisphenol A (BPA) is a common endocrine disruptor found as an environmental and food contaminant. It exerts both developmental and behavioral effects, mainly when exposure occurs in early life. The aim of this study was to determine the multi-generational effects of chronic, human-relevant low-dose exposure to BPA on development, maternal behavior and flavor preference in Wistar rats. BPA was orally administered at a daily dose of 5 µg/kg body weight to F0 pregnant dams from the first day of gestation (GD 1) until the last day of lactation (LD 21), and then to F1 offspring from weaning (PND 21) to adulthood (PND 100). F2 offspring were not exposed. Development and clinical signs of toxicity were assessed daily. Maternal behavior was evaluated by observing nursing and pup-caring actions, as well as "non-maternal" behaviors in F0 and F1 dams from parturition until LD 8. The flavor preferences of F1 and F2 offspring were evaluated based on the intake of sweet, salt and fat solutions using the two-bottle choice test on PND 21-34 and PND 86-99. BPA exposure: 1) decreased maternal behavior in F1 dams, 2) caused developmental defects in both F1 and F2 offspring, with a noticeable decrease in anogenital distance in male rats, and 3) did not affect flavored solution intake in F1, but induced changes in sweet preference in F2 juveniles and in salt and fat solution intakes in F2 adults, and 4) induced a body weight increase in the F2 generation only, whereas food intake and water consumption did not change. Taken as a whole, our findings showed that both gestational (F0) and lifelong (F1) exposures to a human-relevant dose of BPA could induce multi-generational effects on both development and behavior. These results suggest possible selective neuroendocrine defects and/or epigenetic changes caused by BPA exposure.

Sub-NOAEL amounts of vinclozolin and xenoestrogens target rat chondrogenesis in vivo.

Several endocrine disrupting compounds (EDC) elicit skeletal dysgenesis at pharmacological doses. We have investigated the impact of doses below the "No Observed Adverse Effect" (NOAEL) for vinclozolin (V), an anti-androgenic fungicide, alone or associated with xenoestrogens (Genistein, G and bisphenol-A, BPA). V, G, BPA and their combinations were administered orally to female Wistar rats during gestation and lactation. F1 and F2 offspring were investigated for skeletal anomalies at post-natal days 30, 110 (d30, d110). Skeletal development was monitored by measuring caudal vertebrae and long bones dimensions by X-ray micro-CT-scan. A significant increase in Inter Transverse Apophysis (ITA) distance at the upper head of caudal vertebrae, associated with a reduction in vertebral body height was observed in treated F1 females, but not males. Histometrical analysis of vertebral body growth plate cartilage was performed on serial sections of caudal vertebrae. F1 females but not males showed a diminution in growth plate thickness, with greater impact on the hypertrophic zone. All effects were maximal at d30. Effects on ITA width persisted until d110 while effects on growth plate disappeared. These effects were essentially vinclozolin or BPA-dependent. F2 animals were not affected. Our data suggest that vinclozolin and xenoestrogens act as cartilage developmental disruptors. We suggest that present NOAEL values for these compounds, and EDC at large, might be reconsidered using gestational exposure models. Finally, micro CT-scan appears a valuable non-invasive technique to detect EDC effects on live fauna.

Enamel defects reflect perinatal exposure to bisphenol A.

Endocrine-disrupting chemicals (EDCs), including bisphenol A (BPA), are environmental ubiquitous pollutants and associated with a growing health concern. Anecdotally, molar incisor hypomineralization (MIH) is increasing concurrently with EDC-related conditions, which has led us to investigate the effect of BPA on amelogenesis. Rats were exposed daily to BPA from conception until day 30 or 100. At day 30, BPA-affected enamel exhibited hypomineralization similar to human MIH. Scanning electron microscopy and elemental analysis revealed an abnormal accumulation of organic material in erupted enamel. BPA-affected enamel had an abnormal accumulation of exogenous albumin in the maturation stage. Quantitative real-time PCR, Western blotting, and luciferase reporter assays revealed increased expression of enamelin but decreased expression of kallikrein 4 (protease essential for removing enamel proteins) via transcriptional regulation. Data suggest that BPA exerts its effects on amelogenesis by disrupting normal protein removal from the enamel matrix. Interestingly, in 100-day-old rats, erupting incisor enamel was normal, suggesting amelogenesis is only sensitive to MIH-causing agents during a specific time window during development (as reported for human MIH). The present work documents the first experimental model that replicates MIH and presents BPA as a potential causative agent of MIH. Because human enamel defects are irreversible, MIH may provide an easily accessible marker for reporting early EDC exposure in humans.

Oral homeostasis disruption by medical plasticizer component bisphenol A in adult male rats.

OBJECTIVES/HYPOTHESIS: Bisphenol A (BPA) is a synthetic estrogen-like chemical mimetic widely used in the manufacture of polycarbonate plastics and epoxy resins found in numerous consumer products including food packaging, medical devices, and dental sealants. Because it is recovered in fluids and it can reach high levels in saliva, this study aimed to evaluate its safety on oral homeostasis by examining its effects on salivary glands, mouth epithelium, water consumption, and salt preference, each parameter being estrogen sensitive. STUDY DESIGN: Randomized controlled trial involving rats. METHODS: A dose-response study was conducted in adult Wistar rats randomized into five groups (n = 12). BPA was administered over 6 weeks via drinking water to obtain daily dose exposures of 0 µg/kg, 5 µg/kg, 50 µg/kg, 5 mg/kg, and 12.5 mg/kg of body weight. To evaluate salt preference, 1% NaCl solution and pure water intakes were measured for 3 days by offering two-bottle choices. The rats were then killed; oral biopsies were done and submandibular glands were removed for histologic and morphometric analysis. RESULTS: According to the dose-response curve, BPA decreased total drinking but increased salt preference, which was inversely proportional to water consumption (Kruskal-Wallis, P < .01). It also causes oral dryness and histologic changes in the acinar structures of the submandibular glands at the lowest doses (Kruskal-Wallis, P < .01). CONCLUSIONS: This study shows that oral exposure to BPA in the rat disrupts thirst and buccal homeostasis and raises questions about the salivary gland secretions.