Identification of novel bioactive proteins and their produced oligopeptides from Torreya grandis nuts using proteomic based prediction.

Torreya grandis nut is a chief functional food in China consumed for centuries. Besides its rich protein composition, increasing studies are now focusing on T. grandis functional proteins that have not yet identified. In this study, liquid chromatography coupled with mass spectrometry detection of smaller and major proteins, revealed that the major peptide was 36935.00 Da. Proteome sequencing annotated 142 proteins in total. Bioactive proteins such as defensin 4 was annotated and its anti-microbial function was verified. Finally, functional oligopeptides were predicted by searching sequences of digested peptides in databases. Ten group of oligopeptides were suggested to exhibit antioxidant, Angiotensin-converting enzyme inhibition, anti-inflammatory. The predicted antioxidant activity was experimentally validated. It is interesting that a peptide GYCVSDNN digested from defensin 4 showed antioxidant activity. This study reports novel functional peptides from T. grandis nuts that have not been isolated and/or included as functional ingredients in nutraceuticals and in food industry.

Application of deep eutectic solvents in protein extraction and purification.

Deep eutectic solvents (DESs) are a mixture of hydrogen bond donor (HBD) and hydrogen bond acceptor (HBA) molecules that can consist, respectively, of natural plant metabolites such as sugars, carboxylic acids, amino acids, and ionic molecules, which are for the vast majority ammonium salts. Media such as DESs are modular tools of sustainability that can be pointed toward the extraction of bioactive molecules due to their excellent physicochemical properties, their relatively low price, and accessibility. The present review focuses on the application of DESs for protein extraction and purification. The in-depth effects and principles that apply to DES-mediated extraction using various renewable biomasses will be discussed as well. One of the most important observations being made is that DESs have a clear ability to maintain the biological and/or functional activity of the extracted proteins, as well as increase their stability compared to traditional solvents. They demonstrate true potential for a reproducible but more importantly, scalable protein extraction and purification compared to traditional methods while enabling waste valorization in some particular cases.

Preparation of N,S-codoped magnetic bagasse biochar and adsorption characteristics for tetracycline.

Agricultural waste disposal and purification of polluted water are always the key issues of environmental restoration. In this work, thiourea-functionalized magnetic bagasse biochar (MFeBC) was prepared for tetracycline (TC) removal from aqueous solutions. Firstly, MFeBC was prepared by a combined impregnation and chemical coprecipitation method. Furthermore, MFeBC was characterized by Brunauer-Emmett-Teller surface area analysis, Fourier transform infrared spectrometry, X-ray diffraction analysis, scanning electron microscopy, X-ray photoelectron spectroscopy and the magnetic hysteresis curves. For the TC adsorption, the effects of different solution pH level, adsorbent dosage, initial TC concentration and temperature on the adsorption performance were studied respectively. Moreover, the results indicated that the Freundlich isotherm models appropriately described the adsorption process. The kinetic data were better fitted by the pseudo-second-order kinetic model. The maximum TC adsorption capacity of MFeBC reached 69.26 mg g-1. Hydrogen bonding and Π-Π interactions played a dominant role in the adsorption process. Therefore, MFeBC can be used as an effective adsorbent for tetracycline removal from aqueous solution.