An Assessment of Blood Vessel Remodeling of Nanofibrous Poly(ε-Caprolactone) Vascular Grafts in a Rat Animal Model.

The development of an ideal vascular prosthesis represents an important challenge in terms of the treatment of cardiovascular diseases with respect to which new materials are being considered that have produced promising results following testing in animal models. This study focuses on nanofibrous polycaprolactone-based grafts assessed by means of histological techniques 10 days and 6 months following suturing as a replacement for the rat aorta. A novel stereological approach for the assessment of cellular distribution within the graft thickness was developed. The cellularization of the thickness of the graft was found to be homogeneous after 10 days and to have changed after 6 months, at which time the majority of cells was discovered in the inner layer where the regeneration of the vessel wall was found to have occurred. Six months following implantation, the endothelialization of the graft lumen was complete, and no vasa vasorum were found to be present. Newly formed tissue resembling native elastic arteries with concentric layers composed of smooth muscle cells, collagen, and elastin was found in the implanted polycaprolactone-based grafts. Moreover, the inner layer of the graft was seen to have developed structural similarities to the regular aortic wall. The grafts appeared to be well tolerated, and no severe adverse reaction was recorded with the exception of one case of cartilaginous metaplasia close to the junctional suture.

Cyclic Voltammetry Study of Noble Metals and Their Alloys for Use in Implantable Electrodes.

Innovation in the application and miniaturization of implantable electrodes has caused a spike in new electrode material research; however, few robust studies are available that compare different metal electrodes in biologically relevant media. Herein, cyclic voltammetry has been employed to compare platinum, palladium, and gold-based electrodes' potentiometric scans and their corresponding charge storage capacities (CSCs). Ten different noble metals and alloys in these families were tested under pseudophysiological conditions in phosphate-buffered saline (pH 7.4) at 37 °C. Charge storage capacity values (mC/cm2) were calculated for the oxide reduction, hydrogen adsorption, hydrogen desorption, and oxide formation peaks. Five scan rates spanning 2 orders of magnitude (10, 50, 100, 500, and 1000 mV/s) in both sparged and aerated environments were evaluated. Materials have been ranked by their charge storage capacities, reversibility, and trends discussed. Palladium-based alloys outperformed platinum-based alloys in the sparged condition and were ranked equally as high in the aerated condition. The Paliney 1100 (Pd-Re) alloy gave the highest observed calculated CSC value of 0.64 ± 0.02 mC/cm2 in the aerated condition, demonstrating 73 ± 5% reversibility. Trends between metal electrode families elicited in this study can afford valuable insight into future engineering of high performing implantable electrode materials.

Ultra-flat, low-noise, and linearly polarized fiber supercontinuum source covering 670-1390 nm: publisher's note.

This publisher's note contains a correction to Opt. Lett.46, 1820 (2021)10.1364/OL.420676.

Ultra-flat, low-noise, and linearly polarized fiber supercontinuum source covering 670-1390 nm.

We report an octave-spanning coherent supercontinuum (SC) fiber laser with excellent noise and polarization properties. This was achieved by pumping a highly birefringent all-normal dispersion photonic crystal fiber with a compact high-power ytterbium femtosecond laser at 1049 nm. This system generates an ultra-flat SC spectrum from 670 to 1390 nm with a power spectral density higher than 0.4 mW/nm and a polarization extinction ratio of 17 dB across the entire bandwidth. An average pulse-to-pulse relative intensity noise down to 0.54% from 700 to 1100 nm was measured and found to be in good agreement with numerical simulations. This highly stable broadband source could find strong potential applications in biomedical imaging and spectroscopy where an improved signal-to-noise ratio is essential.

Low-noise tunable deep-ultraviolet supercontinuum laser.

The realization of a table-top tunable deep-ultraviolet (UV) laser source with excellent noise properties would significantly benefit the scientific community, particularly within imaging and spectroscopic applications, where source noise has a crucial role. Here we provide a thorough characterization of the pulse-to-pulse relative intensity noise (RIN) of such a deep-UV source based on an argon (Ar)-filled anti-resonant hollow-core (AR HC) fiber. Suitable pump pulses are produced using a compact commercially available laser centered at 1030 nm with a pulse duration of 400 fs, followed by a nonlinear compression stage that generates pulses with 30 fs duration, 24.2 µJ energy at 100 kHz repetition rate and a RIN of < 1%. Pump pulses coupled into the AR HC fiber undergo extreme spectral broadening creating a supercontinuum, leading to efficient energy transfer to a phase-matched resonant dispersive wave (RDW) in the deep-UV spectral region. The center wavelength of the RDW could be tuned between 236 and 377 nm by adjusting the Ar pressure in a 140 mm length of fiber. Under optimal pump conditions the RIN properties were demonstrated to be exceptionally good, with a value as low as 1.9% at ~ 282 nm. The RIN is resolved spectrally for the pump pulses, the generated RDW and the broadband supercontinuum. These results constitute the first broadband RIN characterization of such a deep-UV source and provide a significant step forward towards a stable, compact and tunable laser source for applications in the deep-UV spectral region.

Influence of pulse duration and repetition rate on mid-infrared cascaded supercontinuum.

We experimentally investigate the influence of varying pulse parameters on the spectral broadening, power spectral density, and relative intensity noise of mid-infrared (mid-IR) in-amplifier cascaded supercontinuum generation (SCG) by varying the pulse duration (35 ps, 1 ns, 3 ns) and repetition rate (100, 500, 1000 kHz). The system is characterized at the output of the erbium-ytterbium-doped in-amplifier SCG stage, the thulium/germanium power redistribution stage, and the passive ZBLAN fiber stage. In doing so, we demonstrate that the output of the later stages depends critically on the in-amplifier stage, and relate this to the onset of modulation instability.

Cross-phase modulation instability in PM ANDi fiber-based supercontinuum generation.

We demonstrate broadband supercontinuum generation in an all-normal dispersion polarization-maintaining photonic crystal fiber and report the observation of a cross-phase modulation instability sideband generated outside of the supercontinuum bandwidth. We demonstrate that this sideband is polarized on the slow axis and can be suppressed by pumping on the fiber's fast axis. We theoretically confirm and model this nonlinear process using phase-matching conditions and numerical simulations, obtaining good agreement with the measured data.

In-amplifier and cascaded mid-infrared supercontinuum sources with low noise through gain-induced soliton spectral alignment.

The pulse-to-pulse relative intensity noise (RIN) of near-infrared (near-IR) in-amplifier supercontinuum (SC) sources and mid-IR cascaded SC sources was experimentally and numerically investigated and shown to have significantly lowered noise due to the fundamental effect of gain-induced soliton-spectral alignment. The mid-IR SC source is based on a near-IR in-amplifier SC pumping a cascade of thulium-doped and ZBLAN fibers. We demonstrate that the active thulium-doped fiber not only extend the spectrum, but also to significantly reduce the RIN by up to 22% in the long wavelength region above 2 µm. Using numerical simulations, we demonstrate that the noise reduction is the result of an interplay between absorption-emission processes and nonlinear soliton dynamics leading to the soliton-spectral alignment. In the same way we show that the RIN of the near-IR in-amplifier SC source is already significantly reduced because the spectral broadening takes place in an active fiber that also introduces soliton-spectral alignment. We further show that the low noise properties are transferred to the subsequent fluoride SC, which has a RIN lower than 10% (5%) in a broad region from 1.1-3.6 µm (1.4-3.0 µm). The demonstrated low noise significantly improves the applicability of these broadband sources for mid-IR imaging and spectroscopy.

All-fibre supercontinuum laser for in vivo multispectral photoacoustic microscopy of lipids in the extended near-infrared region.

Among the numerous endogenous biological molecules, information on lipids is highly coveted for understanding both aspects of developmental biology and research in fatal chronic diseases. Due to the pronounced absorption features of lipids in the extended near-infrared region (1650-1850 nm), visualisation and identification of lipids become possible using multi-spectral photoacoustic (optoacoustic) microscopy. However, the spectroscopic studies in this spectral region require lasers that can produce high pulse energies over a broad spectral bandwidth to efficiently excite strong photoacoustic signals. The most well-known laser sources capable of satisfying the multi-spectral photoacoustic microscopy requirements (tunability and pulse energy) are tunable nanosecond optical parametric oscillators. However, these lasers have an inherently large footprint, thus preventing their use in compact microscopy systems. Besides, they exhibit low-repetition rates. Here, we demonstrate a compact all-fibre, high pulse energy supercontinuum laser that covers a spectral range from 1440 to 1870 nm with a 7 ns pulse duration and total energy of 18.3 µJ at a repetition rate of 100 kHz. Using the developed high-pulse energy source, we perform multi-spectral photoacoustic microscopy imaging of lipids, both ex vivo on adipose tissue and in vivo to study the development of Xenopus laevis tadpoles, using six different excitation bands over the first overtone transition of C-H vibration bonds (1650-1850 nm).

Ultra-low-noise supercontinuum generation with a flat near-zero normal dispersion fiber.

A pure silica photonic crystal fiber with a group velocity dispersion (ß2) of 4 ps2/km at 1.55 µm and less than 7 ps2/km from 1.32 µm to the zero dispersion wavelength (ZDW) 1.80 µm was designed and fabricated. The dispersion of the fiber was measured experimentally and found to agree with the fiber design, which also provides low loss below 1.83 µm due to eight outer rings with increased hole diameters. The fiber was pumped with a 1.55 µm, 125 fs laser and, at the maximum in-coupled peak power (P0) of 9 kW, a 1.34-1.82 µm low-noise spectrum with a relative intensity noise below 2.2% was measured. The numerical modeling agreed very well with the experiments and showed that P0 could be increased to 26 kW before noise from solitons above the ZDW started to influence the spectrum by pushing high-noise dispersive waves through the spectrum.

Novel high-strength, low-alloys Zn-Mg (<0.1wt% Mg) and their arterial biodegradation.

It is still an open challenge to find a biodegradable metallic material exhibiting sufficient mechanical properties and degradation behavior to serve as an arterial stent. In this study, Zn-Mg alloys of 0.002 (Zn-002Mg), 0.005 (Zn-005Mg) and 0.08wt% Mg (Zn-08Mg) content were cast, extruded and drawn to 0.25mm diameter, and evaluated as potential biodegradable stent materials. Structural analysis confirmed formation of Mg2Zn11 intermetallic in all three alloys with the average grain size decreasing with increasing Mg content. Tensile testing, fractography analysis and micro hardness measurements showed the best integration of strength, ductility and hardness for the Zn-08Mg alloy. Yield strength, tensile strength, and elongation to failure values of >200-300MPa, >300-400MPa, and >30% respectively, were recorded for Zn-08Mg. This metal appears to be the first formulated biodegradable material that satisfies benchmark values desirable for endovascular stenting. Unfortunately, the alloy reveals signs of age hardening and strain rate sensitivity, which need to be addressed before using this metal for stenting. The explants of Zn-08Mg alloy residing in the abdominal aorta of adult male Sprague-Dawley rats for 1.5, 3, 4.5, 6 and 11months demonstrated similar, yet slightly elevated inflammation and neointimal activation for the alloy relative to what was recently reported for pure zinc.

Clarification of surface modes of a periodic nanopatch metasurface.

We study the angle-dependent optical reflectance spectrum of a metasurface consisting of a periodic array of film-coupled plasmonic nanopatch particles. The nanopatch metasurface exhibits a strong, angle-independent absorption resonance at a wavelength defined by the nanopatch geometry and relative density. When the nanopatches are arranged in a regular lattice, a second, sharp absorption dip is present that varies strongly as a function of the incidence angle. This second resonance is a collective effect involving the excitation of surface plasmon modes and relates to a Wood's anomaly. Using an analytical model, we compute the surface modes of the structure and confirm details about the various mechanisms that contribute to the reflection spectra. The measured reflectance spectra are in excellent agreement with both analytical calculations and full-wave numerical simulations.

Evaluation of wrought Zn-Al alloys (1, 3, and 5 wt % Al) through mechanical and in vivo testing for stent applications.

Special high grade zinc and wrought zinc-aluminum (Zn-Al) alloys containing up to 5.5 wt % Al were processed, characterized, and implanted in rats in search of a new family of alloys with possible applications as bioabsorbable endovascular stents. These materials retained roll-induced texture with an anisotropic distribution of the second-phase Al precipitates following hot-rolling, and changes in lattice parameters were observed with respect to Al content. Mechanical properties for the alloys fell roughly in line with strength (190-240 MPa yield strength; 220-300 MPa ultimate tensile strength) and elongation (15-30%) benchmarks, and favorable elastic ranges (0.19-0.27%) were observed. Intergranular corrosion was observed during residence of Zn-Al alloys in the murine aorta, suggesting a different corrosion mechanism than that of pure zinc. This mode of failure needs to be avoided for stent applications because the intergranular corrosion caused cracking and fragmentation of the implants, although the composition of corrosion products was roughly identical between non- and Al-containing materials. In spite of differences in corrosion mechanisms, the cross-sectional reduction of metals in murine aorta was nearly identical at 30-40% and 40-50% after 4.5 and 6 months, respectively, for pure Zn and Zn-Al alloys. Histopathological analysis and evaluation of arterial tissue compatibility around Zn-Al alloys failed to identify areas of necrosis, though both chronic and acute inflammatory indications were present. © 2017 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 106B: 245-258, 2018.

Biocompatible curcumin loaded PMMA-PEG/ZnO nanocomposite induce apoptosis and cytotoxicity in human gastric cancer cells.

Although curcumin is efficient in killing cancer cells, its poor water solubility and assocaited inadequate bioavailability remain major limitations to its therapeutic application. The formulation of curcumin micellar nanoparticles (NPs) encapsulated with a biodegradable polymer promises to significantly improve curcumin's solubility, stability, and bioavailability. The past decade has witnessed the development of nanoscale curcumin delivery systems: curcumin-loaded liposomes or nanoparticles, self-microemulsifying drug delivery systems (SMEDDS), cyclodextrin inclusions, solid dispersions, nanodisks, and nanotubes. The intention of the present investigation was to enhance the bioavailability and ultimately the efficacy of curcumin by developing a curcumin loaded PMMA-PEG/ZnO bionanocomposite utilizing insoluble curcumin and poorly soluble ZnO nanoparticles. Here, the drug (curcumin) may be carry and deliver the biomolecule(s) by polymer-encapsulated ZnO NPs. Physical characteristics of these novel nanomaterials have been studied with transmission electron microscopy (TEM) and powder X-ray diffraction (XRD) in conjunction with spectral techniques. Aqueous solubility of curcumin was augmented upon conjugation with the polymer-stabilized ZnO NPs. A narrow nanocomposite particle size distribution with an average value of 40 to 90nm was found via TEM. Most importantly, the pH-responsive release of curcumin from the nano-vehicle ensures safer, more controlled delivery of the drug at physiological pH. Cytotoxic potential and cellular uptake of curcumin loaded ZnO NPs were assessed by) cell viability assay, cell cycle assays along with the cell imaging studies have been done in addition to MTT using AGS cancer cells. Hence, these studies demonstrate that the clinical potential of the Curcumin Loaded PMMA-PEG/ZnO can induce the apoptosis of cancer cells through a cell cycle mediated apoptosis corridor, which raises its probability to cure gastric cancer cells.

In Vitro Cytotoxicity, Adhesion, and Proliferation of Human Vascular Cells Exposed to Zinc.

Zinc (Zn) and its alloys have recently been introduced as a new class of biodegradable metals with potential application in biodegradable vascular stents. Although an in vivo feasibility study pointed to outstanding biocompatibility of Zn-based implants in vascular environments, a thorough understanding of how Zn and Zn2+ affect surrounding cells is lacking. In this comparative study, three vascular cell types-human endothelial cells (HAEC), human aortic smooth muscle cells (AoSMC), and human dermal fibroblasts (hDF)-were studied to advance the understanding of Zn/Zn2+-cell interactions. Aqueous cytotoxicity using a Zn2+ insult assay resulted in LD50 values of 50 µM for hDF, 70 µM for AoSMC, and 265 µM for HAEC. Direct cell contact with the metallic Zn surface resulted initially in cell attachment, but was quickly followed by cell death. After modification of the Zn surface using a layer of gelatin-intended to mimic a protein layer seen in vivo-the cells were able to attach and proliferate on the Zn surface. Further experiments demonstrated a Zn dose-dependent effect on cell spreading and migration, suggesting that both adhesion and cell mobility may be hindered by free Zn2+.

Mode-locked Yb-doped fiber laser emitting broadband pulses at ultralow repetition rates.

We report on an environmentally stable, Yb-doped, all-normal dispersion, mode-locked fiber laser that is capable of creating broadband pulses with ultralow repetition rates. Specifically, through careful positioning of fiber sections in an all-PM-fiber cavity mode-locked with a nonlinear amplifying loop mirror, we achieve stable pulse trains with repetition rates as low as 506 kHz. The pulses have several nanojules of energy and are compressible down to ultrashort (<500 fs) durations.

Biodegradable Metals for Cardiovascular Stents: from Clinical Concerns to Recent Zn-Alloys.

Metallic stents are used to promote revascularization and maintain patency of plaqued or damaged arteries following balloon angioplasty. To mitigate the long-term side effects associated with corrosion-resistant stents (i.e., chronic inflammation and late stage thrombosis), a new generation of so-called "bioabsorbable" stents is currently being developed. The bioabsorbable coronary stents will corrode and be absorbed by the artery after completing their task as vascular scaffolding. Research spanning the last two decades has focused on biodegradable polymeric, iron-based, and magnesium-based stent materials. The inherent mechanical and surface properties of metals make them more attractive stent material candidates than their polymeric counterparts. A third class of metallic bioabsorbable materials that are based on zinc has been introduced in the last few years. This new zinc-based class of materials demonstrates the potential for an absorbable metallic stent with the mechanical and biodegradation characteristics required for optimal stent performance. This review compares bioabsorbable materials and summarizes progress towards bioabsorbable stents. It emphasizes the current understanding of physiological and biological benefits of zinc and its biocompatibility. Finally, the review provides an outlook on challenges in designing zinc-based stents of optimal mechanical properties and biodegradation rate.

Corrosion Characteristics Dictate the Long-Term Inflammatory Profile of Degradable Zinc Arterial Implants.

There has been considerable recent interest to develop a feasible bioresorbable stent (BRS) metal. Although zinc and its alloys have many potential advantages, the inflammatory response has not been carefully examined. Using a modified wire implantation model, we characterize the inflammatory response elicited by zinc at high purity (4N) [99.99%], special high grade (SHG)[∼99.7%], and alloyed with 1 wt % (Zn-1Al), 3% (Zn-3Al), and 5.5% (Zn-5Al) aluminum. We found that inflammatory cells were able to penetrate the thick and porous corrosion layer that quickly formed around SHG, Zn-1Al, Zn-3Al, and Zn-5Al implants. In contrast, a delayed entrance of inflammatory cells into the corrosion layer around 4N zinc due to a significantly lower corrosion rate was associated with greater fibrous encapsulation, appearance of necrotic regions, and increased macrophage labeling. Interestingly, cell viability at the interface decreased from SHG, to Zn-1Al, and then Zn-3Al, a trend associated with an increased CD68 and CD11b labeling and capsule thickness. Potentially, the shift to intergranular corrosion due to the aluminum addition increased the activity of macrophages. We conclude that the ability of macrophages to penetrate and remain viable within the corrosion layer may be of fundamental importance for eliciting biocompatible inflammatory responses around corrodible metals.

Influence of Oxygen Concentration on the Performance of Ultra-Thin RF Magnetron Sputter Deposited Indium Tin Oxide Films as a Top Electrode for Photovoltaic Devices.

The opportunity for substantial efficiency enhancements of thin film hydrogenated amorphous silicon (a-Si:H) solar photovoltaic (PV) cells using plasmonic absorbers requires ultra-thin transparent conducting oxide top electrodes with low resistivity and high transmittances in the visible range of the electromagnetic spectrum. Fabricating ultra-thin indium tin oxide (ITO) films (sub-50 nm) using conventional methods has presented a number of challenges; however, a novel method involving chemical shaving of thicker (greater than 80 nm) RF sputter deposited high-quality ITO films has been demonstrated. This study investigates the effect of oxygen concentration on the etch rates of RF sputter deposited ITO films to provide a detailed understanding of the interaction of all critical experimental parameters to help create even thinner layers to allow for more finely tune plasmonic resonances. ITO films were deposited on silicon substrates with a 98-nm, thermally grown oxide using RF magnetron sputtering with oxygen concentrations of 0, 0.4 and 1.0 sccm and annealed at 300 °C air ambient. Then the films were etched using a combination of water and hydrochloric and nitric acids for 1, 3, 5 and 8 min at room temperature. In-between each etching process cycle, the films were characterized by X-ray diffraction, atomic force microscopy, Raman Spectroscopy, 4-point probe (electrical conductivity), and variable angle spectroscopic ellipsometry. All the films were polycrystalline in nature and highly oriented along the (222) reflection. Ultra-thin ITO films with record low resistivity values (as low as 5.83 × 10-4 Ω·cm) were obtained and high optical transparency is exhibited in the 300-1000 nm wavelength region for all the ITO films. The etch rate, preferred crystal lattice growth plane, d-spacing and lattice distortion were also observed to be highly dependent on the nature of growth environment for RF sputter deposited ITO films. The structural, electrical, and optical properties of the ITO films are discussed with respect to the oxygen ambient nature and etching time in detail to provide guidance for plasmonic enhanced a-Si:H solar PV cell fabrication.

Large-Area Metasurface Perfect Absorbers from Visible to Near-Infrared.

An absorptive metasurface based on film-coupled colloidal silver nanocubes is demonstrated. The metasurfaces are fabricated using simple dip-coating methods and can be deposited over large areas and on arbitrarily shaped objects. The surfaces show nearly complete absorption, good off-angle performance, and the resonance can be tuned from the visible to the near-infrared.